Reconstructing the ancient urban landscape in a long-lived city: the Asculum Project, combining research, territorial planning and preventative archaeology

The Asculum Project started in 2012 by the Bologna University in agreement with the former Soprintendenza per iBeni Archeologici delle Marche and the Municipality of Ascoli Piceno, mainly as a project of urban archaeology and preventative archaeology in a city which has been inhabited for a very long period of time. A proper integrated methodology and the combination of a wide range of data, including that gathered from geophysical surveys, archaeological digs, historic cartography, bibliographic and archival data, allowed us to reconstruct the cityscape during the Roman Age and its development over the centuries. The understanding of the ancient urban landscape also included a detailed morphological study aimed at the reconstruction of the Roman paleosurface, carried out using data derived from coring samples and stratigraphic digs. In parallel, particular attention was directed to the modern 3D documentation of the historical buildings of the city, by means of laser scanner and the analysis of the stratigraphy of the surviving walls. The new surveys covered, in particular, the still extant Roman buildings, such as the temples incorporated by the churches of San Venanzio and San Gregorio Magno, as well as the Sostruzioni dell’Annunziata. These last acquisitions made it possible to reconstruct the overall layout and urban plan of the town during the Roman Age, as well as to shed new light on the conformation of the ancient landscape at the time of the oldest Piceni settlement. One of the most interesting aspects of the operating practices applied in the project was to reconcile the needs for preservation and research with the aim of a sustainable urban development

The Compound Muscle Action Potential as Neurophysiological Marker for Amyotrophic Lateral Sclerosis.

Objectives: To definite the peripheral nervous involvement in ALS through the repeated use of the compound motor action potential (CMAP) to test the progression of disease, to determine different change of phrenic CMAP and forced vital capacity (FVC) in spinal and bulbar onset, and to establish clinical and neurophysiological features of patients with poor prognosis. Material & Methods: CMAP from phrenic, ulnar, and medial plantar nerves, Medical Research Council (MRC) score, revised ALS functional rating scale (ALSFRS-R) and FVC were evaluated in 117 ALS patients every three months in one year-period. Results: Bulbar onset patients had lower FVC but similar amplitude of phrenic CMAP at baseline compared to spinal onset patients. The patients with poor prognosis had lower phrenic CMAP and FVC at baseline. CMAP values, when compared to the rate found in the previous visit, reduced significantly in both poor and good prognosis groups during the entire follow-up period, while the FVC reduced significantly only in the first three months. Conclusions: CMAP is a reproducible sensitive marker for motor neurons loss and collateral reinnervation in ALS also in a short period of time. The changes in CMAP, MRC, FVC and ALSFRS-R score resulted correlated, but CMAP is the only parameter with the advantage to demonstrate objectively the progression of disease in both patients with poor and good prognosis for the entire period of follow-up. It should be used as clinical outcome of ALS in clinical trials, taking advantage of its objectivity and selectivity for peripheral nervous system study

NGF-response of EGF-dependent progenitor cells obtained from human sympathetic ganglia

SIGNALLING molecules are thought to play a significant role in determining the fate of neural crest progenitor cells. The human sympathetic chain was identified at 6.5, 7.5, 8.2, 10.2 and 11.4 postconception (PC) weeks demonstrating low affinity nerve growth factor (NGF) receptors, and was processed for tissue culture. In the presence of epidermal growth factor (EGF), floating spheres of proliferating progenitor cells were developed in vitro. In the absence of EGF progenitor cells differentiated into tyrosine hydroxylase (TH)-immunoreactive neuronal and TH-negative flat cells. NGF treatment significantly increased neurite outgrowth and survival of TH-immunoreactive cells. The multipotent cells we isolated differ from previously reported sympathoadrenal progenitors in that they give rise to TH immunoreactive neurones precociously sensitive to NGF

Seeing into the past: integrating 3D documentation and non-invasive prospecting methods for the analysis, understanding and reconstruction of the ancient Pompeii. The case of the House of Obellio Firmo (IX, 14)

In 2015 the Department of History and Cultures of the Bologna University took part in the Grande Progetto Pompei - Piano della Conoscenza, with the task of providing a modern and complete documentation of the so-called Lotto 3 in Pompeii. The new survey was carried out by means of integrated innovative diagnostic survey techniques in order to provide a total documentary research of the whole sector. In 2016 a new project was started in agreement with the competent Superintendency, and focused on the study and preservation of the House of Obellio Firmo, included in the Lotto 3 of the Roman city. The new research contemplates an in-depth analysis of the building, employing systematic laser scanning and photogrammetry methods to generate an accurate 3D model of the house. This model is going to constitute the starting point for the further analysis of the wall stratigraphies and for the mapping and monitoring of the structures’ state of decay. The full-scale analytical documentation of the building also includes a detailed geophysical mapping of all the accessible domestic spaces, by using the ground penetrating radar technique. The preliminary results achieved by the non-invasive prospecting survey, integrated with the analysis of the surviving walls and building techniques, supply valid information for the archaeological interpretation of the house’s history. In order to allow the management and sharing of the information collected, the data are going to be organised within a building information model (BIM) with a triple objective: the reconstruction of a fragment of the ancient urban landscape in Pompeii during the oldest phase, with particular attention directed to the Samnitic period; the outlining of a precise strategy of intervention for the restoration and preservation of the House of Obellio Firmo; the re-opening of the building to sightseeing tours and its restitution to public use

Tharros - Capo San Marco in the Phoenician and Punic Age. Geophysical investigations and virtual rebuilding

The project described in this paper was started in 2012 and concerns the study of the relationship between the urban and suburban districts of the Phoenician and Punic city of Tharros (Cabras, Oristano). The structures of Phoenician and Punic Tharros have been largely cancelled by the Roman occupation. For this reason it is very difficult to determine the original function of many of the neighbourhoods during the Carthaginian period. The archaeological excavation primarily involved the southern necropolis of Capo San Marco. The cemetery must still be fully explored and understood under several aspects, mainly because of the devastation of the site caused by the repeated plundering of the ancient tombs which occurred during the 19th century. In addition to the new dig activities, a 3D topographical survey aimed at the complete documentation of the site and at the virtual rebuilding of the Phoenician and Punic funerary landscape was completed. Another goal of the project is the insertion of this sector of the promontory into the usual tourist route, in order to foster the public fruition of Capo San Marco, while continuing to adopt proper scientific methods and modern techniques. In this direction, geophysical prospecting surveys were carried out in the southern sector of the Capo San Marco, near the so-called ‘Rustic Temple’, in order to assess human presence in the farthest point of the Sinis peninsula (characterised by the presence of the Late Punic ruins of a probable light-house with sacred functions), and across the whole isthmus Sa Codriola towards the hill of San Giovanni, with the aim of analysing the northern boundary of the cemetery and its relationship to the city. The Punic-Roman settlement is now enclosed in the archaeological park, which is a fraction of what was supposed to be the administrative capital of Carthage in Sardinia. 3D modelling and virtual reconstructions were focused also on the residential Punic and Roman area inside the park. The integrated application of the most advanced topographical and geophysical techniques to the site greatly contributed to the recording and understanding of the ancient landscape

Empathic brain responses in insula are modulated by levels of alexithymia but not autism

Difficulties in social cognition are well recognized in individuals with autism spectrum conditions (henceforth ‘autism'). Here we focus on one crucial aspect of social cognition: the ability to empathize with the feelings of another. In contrast to theory of mind, a capacity that has often been observed to be impaired in individuals with autism, much less is known about the capacity of individuals with autism for affect sharing. Based on previous data suggesting that empathy deficits in autism are a function of interoceptive deficits related to alexithymia, we aimed to investigate empathic brain responses in autistic and control participants with high and low degrees of alexithymia. Using functional magnetic resonance imaging, we measured empathic brain responses with an ‘empathy for pain' paradigm assessing empathic brain responses in a real-life social setting that does not rely on attention to, or recognition of, facial affect cues. Confirming previous findings, empathic brain responses to the suffering of others were associated with increased activation in left anterior insula and the strength of this signal was predictive of the degree of alexithymia in both autistic and control groups but did not vary as a function of group. Importantly, there was no difference in the degree of empathy between autistic and control groups after accounting for alexithymia. These findings suggest that empathy deficits observed in autism may be due to the large comorbidity between alexithymic traits and autism, rather than representing a necessary feature of the social impairments in autis

A simple circular cell method for multilevel finite element analysis

A simple multiscale analysis framework for heterogeneous solids based on a computational homogenization technique is presented. The macroscopic strain is linked kinematically to the boundary displacement of a circular or spherical representative volume which contains the microscopic information of the material. The macroscopic stress is obtained from the energy principle between the macroscopic scale and the microscopic scale. This new method is applied to several standard examples to show its accuracy and consistency of the method proposed

Produção, teor e quantidade acumulada de nutrientes em rabanete cultivado em diferentes substratos.

O objetivo deste trabalho foi avaliar a produção, teor e quantidade acumulada de nutrientes em rabanete nos substratos areia, fibra de casca de coco (fcc) e mistura de areia com fibra de casca de coco.Resumo 963

NMR Metabolomics for Stem Cell type discrimination

Cell metabolism is a key determinant factor for the pluripotency and fate commitment of Stem Cells (SCs) during development, ageing, pathological onset and progression. We derived and cultured selected subpopulations of rodent fetal, postnatal, adult Neural SCs (NSCs) and postnatal glial progenitors, Olfactory Ensheathing Cells (OECs), respectively from the subventricular zone (SVZ) and the olfactory bulb (OB). Cell lysates were analyzed by proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy leading to metabolites identification and quantitation. Subsequent multivariate analysis of NMR data by Principal Component Analysis (PCA), and Partial Least Square Discriminant Analysis (PLS-DA) allowed data reduction and cluster analysis. This strategy ensures the definition of specific features in the metabolic content of phenotypically similar SCs sharing a common developmental origin. The metabolic fingerprints for selective metabolites or for the whole spectra demonstrated enhanced peculiarities among cell types. The key result of our work is a neat divergence between OECs and the remaining NSC cells. We also show that statistically significant differences for selective metabolites characterizes NSCs of different ages. Finally, the retrived metabolome in cell cultures correlates to the physiological SC features, thus allowing an integrated bioengineering approach for biologic fingerprints able to dissect the (neural) SC molecular specificitie

Inefficient skeletal muscle oxidative function flanks impaired motor neuron recruitment in Amyotrophic Lateral Sclerosis during exercise

This study aimed to evaluate muscle oxidative function during exercise in amyotrophic lateral sclerosis patients (pALS) with non-invasive methods in order to assess if determinants of reduced exercise tolerance might match ALS clinical heterogeneity. 17 pALS, who were followed for 4 months, were compared with 13 healthy controls (CTRL). Exercise tolerance was assessed by an incremental exercise test on cycle ergometer measuring peak O2 uptake ([Formula: see text]O2peak), vastus lateralis oxidative function by near infrared spectroscopy (NIRS) and breathing pattern ([Formula: see text]E peak). pALS displayed: (1) 44% lower [Formula: see text]O2peak vs. CTRL (p\u2009<\u20090.0001), paralleled by a 43% decreased peak skeletal muscle oxidative function (p\u2009<\u20090.01), with a linear regression between these two variables (r2\u2009=\u20090.64, p\u2009<\u20090.0001); (2) 46% reduced [Formula: see text]Epeak vs. CTRL (p\u2009<\u20090.0001), achieved by using an inefficient breathing pattern (increasing respiratory frequency) from the onset until the end of exercise. Inefficient skeletal muscle O2 function, when flanking the impaired motor units recruitment, is a major determinant of pALS clinical heterogeneity and working capacity exercise tolerance. CPET and NIRS are useful tools for detecting early stages of oxidative deficiency in skeletal muscles, disclosing individual impairments in the O2 transport and utilization chain