Effects of astaxanthin-enriched yeast on mucosal IgA induction in the jejunum and ileum of weanling mice.

The present study was conducted to clarify the effects of astaxanthin-enriched yeast on the concentration of immunoglobulin A (IgA), the numbers of IgA antibody-secreting cells (ASC) and the messenger RNA (mRNA) expression of IgA C-region in the jejunum and ileum of weanling mice. Weanling mice were fed rodent feed or astaxanthin-enriched yeast-supplemented rodent feed for 7, 14 or 21 days. Supplemental astaxanthin-enriched yeast increased the numbers of IgA ASC in the jejunum and ileum after 7, 14 and 21 days of treatment. Supplemental astaxanthin-enriched yeast increased IgA concentrations in the jejunum after 21 days of treatment, but IgA concentrations in the ileum were not affected by the treatment. The mRNA expressions of IgA C-region in the jejunum after 14 and 21 days of treatment and the ileum after 14 days of treatment were enhanced by supplementation of astaxanthin-enriched yeast. These results indicate that supplementation of astaxanthin-enriched yeast is effective to enhance the numbers of IgA ASC in the jejunum and ileum and IgA concentrations in the ileum of weanling mice

Effects of Simulated Angler Capture and Live-Release Tournaments on Walleye Survival

We examined the effects of acclimation water temperature, live-well (LW) water temperature, and LW dissolved oxygen (DO) concentration on survival of adult Walleyes Sander vitreus subjected to simulated tournament conditions (angling, LW confinement, and weigh-in procedures) under controlled laboratory conditions. We tested three acclimation temperatures (12, 18, and 24◦C), and three LWtemperature differentials (Delta T=−4, 0, and +4◦C) were tested at each acclimation temperature. Survival was monitored after 8 h of LW confinement and during a 5-d retention period in 1,700-L tanks. None of the Walleyes that were acclimated to 24◦C and subjected to simulated tournament procedures survived the 5-d retention period; for fish subjected only to simulated angling at 24◦C, survival during the 5-d retention period was 29%. Five-day survival was generally over 70% at acclimation temperatures of 12◦C and 18◦C, and we observed a significant interaction between acclimation temperature and Delta T; survival was greatest in LWs at −4◦C Delta T for fish acclimated to 18◦C and in LWs at +4◦C Delta T for fish acclimated to 12◦C. Best survival of Walleyes subjected to the stress of angling and tournament procedures was obtained at temperatures 6–8◦Cbelow the optimum temperature for adult Walleyes (i.e., optimum = 20–22◦C). Five-day survival exceeded 70% when LW DO was 5 or 12–15 mg/L (at an acclimation and LW temperature of 18◦C), but survival was 0% when DO was 2 mg/L. Anglers may increase survival of Walleyes through careful manipulation of LW temperature and DO when ambient temperature is at or below 18◦C, but high mortality of angled and LW-retained Walleyes should be expected when ambient water temperatures are 24◦C or greater

Relationships between immunoglobulin and fat-soluble vitamins in colostrum of Japanese Black multiparous cows.

Data from 19 Japanese Black multiparous cows were collected to clarify the relationships among immunoglobulin (Ig) G, IgA, β-carotene, vitamin A and α-tocopherol contents in colostrum of cows in order to evaluate the role of fat-soluble vitamins on colostral IgG and IgA production. Mean colostral IgG was 141 mg/mL, ranging from 65 to 208 mg/mL, whereas mean colostral IgA was 8.7 mg/mL, ranging from 1.0 to 34.6 mg/mL. Colostral IgG increased with aging in multiparous cows. There were positive correlations between colostral IgG and colostral vitamin A or colostral α-tocopherol in cows, and the higher adjusted R(2) was obtained in the prediction model of colostral IgG from age and colostral vitamin A. Colostral vitamin A was positively correlated with colostral β-carotene or colostral α-tocopherol in cows, but there were no relationships between colostral IgA and colostral IgG or colostral fat-soluble vitamins. These results indicate that fat-soluble vitamin contents in colostrum of cows may change in similar patterns and high colostral vitamin A is related with high colostral IgG