Filtration efficiency of nonofiber cloth

V této bakalářské práci se zabývám filtrační účinností tkaniny z nanovlákna, která se stanoví pomocí počtu částic aerosolu naměřených před a za filtrem pomocí přístroje APS. Aerosol je generován přístrojem CMAG. Dále se v této práci zabývám rešeršní studií na téma filtrace částic mikrometrových rozměrů.In this bachelor thesis I deal with filtration efficiency of nanofiber cloth, which are determined by number of aerosol particles measured in front of the filter and behind the filter using APS. Aerosol particles are generated by CMAG. The next topic of this thesis is a search of particle filtration of micrometer size.

Multifunctional House

Úkolem této bakalářské práce je zpracovat částečnou dokumentaci pro provádění stavby dle vyhlášky č. 499/2006 Sb. (ve znění pozdějších předpisů) o dokumentaci staveb. Tato dokumentace je zpracovaná pro multifunkční dům, který navazuje na projekt zpracovávaný v předmětech Ateliérová tvorba III. (urbanistická studie), Ateliérovou tvorbu IV (architektonická studie) a Ateliérovou tvorbu Va (dokumentace pro stavební povolení). Hlavním cílem bylo navrhnout multifunkční objekt, který bude vstupní dominantou při vjezdu do Městského Obvodu Nová Ves – Statutárního města Ostravy.The task of this bachelor's thesis is the elaboration of partial documentation for the execution of the project according to regulation n. 499/2006 Coll. (as amended) on building documentation. This documentation is processed for a multifunctional house, which follows the project elaborated in the Studio work III. (urban studies), Studio work IV. (architectural study) and Studio work Va (building permit documentation). The main objective was to design a multifunctional building, which dominates the entrance at the entrance to the city District Nová Ves - Statutory city of Ostrava.226 - Katedra architekturyvelmi dobř

The Women in Science in the Countries of European Union

We live in a time, when few people concern of that, why is so few women in the science; although nowadays is coequality between men and women. We would not to forget the reality; that women could have to study on the universities only from the 19th century. That could be one of the reasons, why nowadays is so few women in science. Certainly, the other reason is the fact; that women in history didn´t signify more in the society. Their maximal success in their life was to perform the position of the mother and wife. Their role was the nascence children, so that the parentage would be well-preserved and would not threaten the decrease of population; and not last one reason was the care about the household. The resource to employ was the utopian soon. The man was the bread-winner. In 19th century were the women too, those attended the educational institutions, the private or the public too. They were the women; those had to perform the wife-position of some officer, higher positioned man, in the future. The thinkers of enlightenment performed the opinion, that women can´t study and to develop the science, because they are very emotional. This description belongs to antipoles in the science, those the scientists’ couldn´t have to know. The most important in the science is the rationality and the objectivity; and especially these descriptions have men better developed

Arabidopsis mutants reveal multiple singlet oxygen signaling pathways involved in stress response and development

Shortly after the release of singlet oxygen (1O2) in chloroplasts drastic changes in nuclear gene expression occur in the conditional flu mutant of Arabidopsis that reveal a rapid transfer of signals from the plastid to the nucleus. Factors involved in this retrograde signaling were identified by mutagenizing a transgenic flu line expressing a 1O2-responsive reporter gene. The reporter gene consisted of the luciferase open reading frame and the promoter of an AAA-ATPase gene (At3g28580) that was selectively activated by 1O2 but not by superoxide or hydrogen peroxide. A total of eight second-site mutants were identified that either constitutively activate the reporter gene and the endogenous AAA-ATPase irrespectively of whether 1O2 was generated or not (constitutive activators of AAA-ATPase, caa) or abrogated the 1O2-dependent up-regulation of these genes as seen in the transgenic parental flu line (non-activators of AAA-ATPase, naa). The characterization of the mutants strongly suggests that 1O2-signaling does not operate as an isolated linear pathway but rather forms an integral part of a signaling network that is modified by other signaling routes and impacts not only stress responses of plants but also their developmen

Kurz práce s informacemi

Skripta ke kurzu KPI11, která popisují všechna základní témata informačního vzdělávání – informační sebeřízení, definování informační potřeby, hledání informací, organisace poznatků, analýza, tvorba dokumentů, tvorba znalostí či komunikace

Phenotypic plasticity in haptoral structures of Ligophorus cephali (Monogenea: Dactylogyridae) on the flathead mullet (Mugil cephalus): A Geometric Morphometric Approach

Evaluating phenotypic plasticity in attachment organs of parasites can provide information on the capacity to colonise new hosts and illuminate evolutionary processes driving host specificity. We analysed the variability in shape and size of the dorsal and ventral anchors of Ligophorus cephali from Mugil cephalus by means of geometric morphometrics and multivariate statistics. We also assessed the morphological integration between anchors and between the roots and points in order to gain insight into their functional morphology. Dorsal and ventral anchors showed a similar gradient of overall shape variation, but the amount of localised changes was much higher in the former. Statistical models describing variations in shape and size revealed clear differences between anchors. The dorsal anchor/bar complex seems more mobile than the ventral one in Ligophorus, and these differences may reflect different functional roles in attachment to the gills. The lower residual variation associated with the ventral anchor models suggests a tighter control of their shape and size, perhaps because these anchors seem to be responsible for firmer attachment and their size and shape would allow more effective responses to characteristics of the microenvironment within the individual host. Despite these putative functional differences, the high level of morphological integration indicates a concerted action between anchors. In addition, we found a slight, although significant, morphological integration between roots and points in both anchors, which suggests that a large fraction of the observed phenotypic variation does not compromise the functional role of anchors as levers. Given the low level of genetic variation in our sample, it is likely that much of the morphological variation reflects host-driven plastic responses. This supports the hypothesis of monogenean specificity through host-switching and rapid speciation. The present study demonstrates the potential of geometric morphometrics to provide new and previously unexplored insights into the functional morphology of attachment and evolutionary processes of host¿parasite coevolution

A major invasion of transposable elements accounts for the large size of the Blumeria graminis f.sp. tritici genome

Powdery mildew of wheat (Triticum aestivum L.) is caused by the ascomycete fungus Blumeria graminis f.sp. tritici. Genomic approaches open new ways to study the biology of this obligate biotrophic pathogen. We started the analysis of the Bg tritici genome with the low-pass sequencing of its genome using the 454 technology and the construction of the first genomic bacterial artificial chromosome (BAC) library for this fungus. High-coverage contigs were assembled with the 454 reads. They allowed the characterization of 56 transposable elements and the establishment of the Blumeria repeat database. The BAC library contains 12,288 clones with an average insert size of 115kb, which represents a maximum of 7.5-fold genome coverage. Sequencing of the BAC ends generated 12.6Mb of random sequence representative of the genome. Analysis of BAC-end sequences revealed a massive invasion of transposable elements accounting for at least 85% of the genome. This explains the unusually large size of this genome which we estimate to be at least 174Mb, based on a large-scale physical map constructed through the fingerprinting of the BAC library. Our study represents a crucial step in the perspective of the determination and study of the whole Bg tritici genome sequenc

BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes

The assembly of a reference genome sequence of bread wheat is challenging due to its specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by the International Wheat Genome Sequencing Consortium as the key strategy, reduces problems caused by the genome complexity and polyploidy, but the repeat content still hampers the sequence assembly. Availability of a high-resolution genomic map to guide sequence scaffolding and validate physical map and sequence assemblies would be highly beneficial to obtaining an accurate and complete genome sequence. Here, we chose the short arm of chromosome 7D (7DS) as a model to demonstrate for the first time that it is possible to couple chromosome flow sorting with genome mapping in nanochannel arrays and create a de novo genome map of a wheat chromosome. We constructed a high-resolution chromosome map composed of 371 contigs with an N50 of 1.3 Mb. Long DNA molecules achieved by our approach facilitated chromosome-scale analysis of repetitive sequences and revealed a ~800-kb array of tandem repeats intractable to current DNA sequencing technologies. Anchoring 7DS sequence assemblies obtained by clone-by-clone sequencing to the 7DS genome map provided a valuable tool to improve the BAC-contig physical map and validate sequence assembly on a chromosome-arm scale. Our results indicate that creating genome maps for the whole wheat genome in a chromosome-by-chromosome manner is feasible and that they will be an affordable tool to support the production of improved pseudomolecules