降低UF胶粘剂游离甲醛含量的研究

研究了甲醛与尿素的配比[n(F)/n(U)比值]、缩聚温度、时间与pH值,以及改性剂对以尿素、甲醛为主要原料制备脲醛(UF)树脂胶粘剂游离甲醛含量的影响,获得了确保其粘接强度不致下降,而游离甲醛含量只有0.25%的最佳制备工艺条件

一种采用固体多相催化剂的用于烯烃氢甲酰化反应的方法

本发明涉及一种用于烯烃氢甲酰化反应的方法，其特征在于，所述方法采用一种由金属组分和具有多级孔结构的有机配体聚合物组成的固体多相催化剂，其中所述金属组分是金属Rh、Ir或Co中的一种或几种，所述有机配体聚合物是含有P和烯烃基以及任选的N的有机配体单体经聚合后形成的聚合物，在所述固体多相催化剂中，所述金属组分与所述有机配体聚合物骨架中的P原子或N形成配位键并且以单原子分散的状态存在，所述方法包括在所述固体多相催化剂存在下使烯烃原料与CO／H2混合气在反应器中进行所述烯烃氢甲酰化反应。该方法使用新型固体多相催化剂，反应工艺及装置简单，催化剂的氢甲酰化性能稳定，活性和收率高，具有广阔的工业应用前景

一种乙烯氢甲酰化制丙醛的多相催化剂及使用其的方法

本发明涉及一种乙烯氢甲酰化制丙醛的多相催化剂及使用该催化剂的固定鼓泡床反应工艺。更具体地，所述多相催化剂通过有机配体聚合物载体自负载过渡金属组分形成，其中所述有机配体聚合物载体是通过溶剂热聚合法聚合含有P和任选的N并且含有烯基的有机配体单体形成的并且具有多级孔结构，所述过渡金属组分是Rh、Ir或Co中的一种或几种，并且基于所述催化剂的总重量，所述过渡金属组分的含量为1～20.0％。本发明催化剂的制备方法、应用其的反应工艺及装置简单，催化剂的乙烯氢甲酰化制丙醛性能稳定，活性和收率高，实现了的强放热的乙烯氢甲酰化反应，具有广阔的工业应用前景

非晶态固体冲击波响应的介尺度模拟

非晶态固体不存在位错运动等传统晶体塑性机制,而是以原子团簇在介尺度的“剪切转变”诱导塑性变形.由于时空尺度限制,研究剪切转变在动态冲击作用下的动力学过程十分困难,因此非晶态固体冲击波响应的物理规律还不清楚.为此,本文基于剪切转变动力学建立了动态介尺度模型,结合有限元模拟,系统研究了非晶态固体的弹塑性冲击波响应随冲击速度和传播距离的演化规律.结果表明,随着冲击速度的增大,剪切转变激活逐渐增多且以塑性波模式逐渐追上弹性波前沿.剪切转变塑性波与加载波的相互作用导致Hugoniot弹性极限随传播距离呈现指数衰减,以及高速冲击时塑性前沿更快速地上升.通过改变剪切转变本征应变,本文还研究了塑性冲击前沿应变率随稳态冲击压缩Hugoniot态应力的非4次幂律关系,发现该幂律指数与非晶态固体到达Hugoniot弹性极限前内部剪切转变激活的非线性增殖密切相关

theseparationandenrichmentofglycoproteinsorglycopeptidesbasedonnanoparticles

As one of the most important post-translational modifications, protein glycosylation has a significant effect on the structures and functions of proteins. Many low-abundance endogenous glycoproteins/glycopeptides in the serum or tissue extracts are biomarkers with higher clinical sensitivity and specificity, which could provide valuable information to explore the pathogenesis of many diseases, discover disease biomarkers and develop proteins based new drugs. However, it is still a great challenge for glycoproteomics to selectively separate and enrich the glycoproteins/glycopeptides from the complex biological samples due to their extremely low concentrations. Nanoparticle materials have been attracted much attention in the separation and enrichment of glycoproteins/glycopeptides due to their large surface to volume ratio, numerous affinity sites and unique structures. In this work, the recent progress for the separation and enrichment of glycoproteins/glycopeptides based on the nanoparticles, including gold, SiO2, TiO2, nanodiamond and polymer nanoparticles are reviewed. Finally, the challenges and prospects for the methods of the separation and enrichment of glycoproteins/glycopeptides are briefly proposed

theseparationandenrichmentofglycoproteinsorglycopeptidesbasedonnanoparticles

As one of the most important post-translational modifications, protein glycosylation has a significant effect on the structures and functions of proteins. Many low-abundance endogenous glycoproteins/glycopeptides in the serum or tissue extracts are biomarkers with higher clinical sensitivity and specificity, which could provide valuable information to explore the pathogenesis of many diseases, discover disease biomarkers and develop proteins based new drugs. However, it is still a great challenge for glycoproteomics to selectively separate and enrich the glycoproteins/glycopeptides from the complex biological samples due to their extremely low concentrations. Nanoparticle materials have been attracted much attention in the separation and enrichment of glycoproteins/glycopeptides due to their large surface to volume ratio, numerous affinity sites and unique structures. In this work, the recent progress for the separation and enrichment of glycoproteins/glycopeptides based on the nanoparticles, including gold, SiO2, TiO2, nanodiamond and polymer nanoparticles are reviewed. Finally, the challenges and prospects for the methods of the separation and enrichment of glycoproteins/glycopeptides are briefly proposed

高效液相色谱串联质谱法与化学发光法定量测定 女性血清总睾酮的比较分析

【目的】通过比较高效液相色谱串联质谱法（LC-MS/MS）与化学发光法（CLIA）对多囊卵巢综合征 （PCOS）患者及健康对照女性血清总睾酮（TT）的定量检测，探索LC-MS/MS 诊断生化高雄激素血症及PCOS 的 临床意义。【方法】收集PCOS 患者325 例、正常对照者244 例，比较病例组与对照组及各亚组间LC-MS/MS 和 CLIA 测定的血清总睾酮。【结果】LC-MS/MS 法可明显区分PCOS 患者与正常女性的TT 水平。LC-MS/MS 法TT 及相应游离睾酮指数（FAI）在多毛组明显高于非多毛组，而CLIA 法TT 及相应FAI 在两组间差异不显著。LCMS/ MS 法TT 与多毛评分（mFG）呈正等级相关，而CLIA 法TT 与mFG 则无线性等级相关。Bland-Altman 法和 Deming 回归分析均提示LC-MS/MS 法和CLIA 法检测女性血清TT 的一致性欠佳。LC-MS/MS 法TT 诊断高雄激 素血症截断值为≥ 1.85 nmol/L。CLIA 法TT 诊断高雄激素血症截断值为≥ 2.39 nmol/L。LC-MS/MS 高雄组与 CLIA高雄组间身体测量参数及实验室检查均有较多差异（P < 0.05）。ROC曲线亦提示LC-MS/MS法TT测定对 PCOS 有好的诊断价值。【结论】LC-MS/MS 法测定血清TT较CLIA 灵敏性高、准确度好，对女性生化高雄激素血 症与PCOS的诊断效能高

基于bodipy母核的羧酸酯酶1特异性荧光探针底物的设计研发

羧酸酯酶1 (carboxylesterase 1, CE1)是哺乳动物体内分布的一种重要的丝氨酸水解酶,广泛参与多种内外源性酯类化合物(包括胆固醇酯等内源物,以及酯类药物及杀虫剂等外源物)的水解代谢。本研究基于人羧酸酯酶1 (hCE1)偏好底物的结构特征,选取8-羧酸-BODIPY为荧光母核,设计合成了4种BODIPY-8-羧酸酯衍生物,进而通过单酶筛选和酶抑制实验考察了hCE1催化4种酯类底物的特异性。研究发现, BODIPY-8-羧酸酯的醇基部分越小, hCE1对底物的选择性越高, BODIPY-8-羧酸甲酯(BCM)和BODIPY-8-羧酸乙酯(BCE)均可以作为hCE1的特异性荧光探针底物。在此基础上,选择了水解速率更快的BCM为hCE1探针底物,进一步考察了BCM在人肝微粒体(HLM)和hCE1单酶中的水解动力学,并借助该底物开展了hCE1抑制剂的高效表征研究。研究发现BCM在HLM和hCE1中的酶动力学行为及Km值非常接近,表明hCE1是HLM中参与BCM水解的主要代谢酶。此外,抑制剂表征实验表明BCM可用于hCE1抑制剂的高效筛选与评价,且可用HLM代替hCE1单酶进行酶抑制剂的筛选与评价