利用草鱼原代肝细胞培养评价二噁毒性效应

利用草鱼原代肝细胞培养技术评价二口恶 口英 毒性效应,测定结果与离体培养生物测试方法、高分辨质谱分析方法的测定结果进行了比较.研究结果表明,同步获得的2,3,7,8-四氯代二苯并二口恶 口英 (2,3,7,8-TCDD)对草鱼原代肝细胞和离体H4IIE细胞的EROD酶活力诱导的剂量-效应关系标准曲线,前者的EC50值比后者的EC50值高出约10倍,草鱼原代肝细胞对2,3,7,8-TCDD的灵敏度比离体H4IIE细胞的要低,2,3,7,8-TCDD对这两种细胞生物毒性效应存在着差异.测定同一环境样品中的二

利用草鱼原代细胞培养技术测定二恶英的毒性效应

原代培养是从活体动物获取组织细胞后在体外进行的首次培养,其最大优点是细胞刚刚离体,生物性状尚未发生很大变化,具有二倍体的遗传性,最接近和反映体内生长特性,且大多数细胞表现出原有组织的特性,很适合作为毒物测试、细胞分化及病理学方面的试验研究。原代细胞试验介于活体试验和离体试验之间,使活体试验条件归一简单化而不失其真实性,使离体试验更接近实际而使实验结果可以直接外推到活体生物。原代培养技术为建立介于内源代谢和外源代谢之间的生物分析模型提供了可能性,弥补了离体实验和活体实验的不足。本论文以二恶英为模型化合

利用草鱼原代肝细胞培养评价二恶英毒性效应

利用草鱼原代肝细胞培养技术评价二恶英毒性效应，测定结果与离体培养生物测试方法，高分辨质谱分析方法的测定结果进行了比较，研究效果表明，同步获得的2，3，7，8－四氯代二苯并二恶英（2，3，78－TCDD）对草鱼原代肝细胞和离体H41IE细胞的EROD酶活力诱导的剂量－效应关系标准曲线，前者的EC50值比后者的EC50值高出的10倍，草鱼原代肝细胞对2，3，7，8－TCDD的灵敏度比离体H4IIE细胞的要低2，3，7，8－TCDD对这两种细胞生物毒性效应存在着差异，测定同一环境样品中的二恶英类化合物毒性时，离体培养方法的测定结果比原代培养方法的测定结果更接近化学分析结果，但原代培养比离体培养更能如实地反映环境中污染物的生物毒性和毒理动力学过程

稀有（鱼句）鲫卵黄蛋白原电泳表征

鱼类卵黄蛋白原是检测环境内分泌干扰化合物雌激素效应的敏感生物标志物.稀有(鲍)鲫是我国目前最具发展潜力的用于生态毒理学研究的实验室模型物种.本文采用强效人工合成的雌激素乙炔基雌二醇对成熟雄性稀有(鲍)鲫和未成年稀有(鲍)鲫进行诱导,然后用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳对血清中的蛋白进行了表征,发现雌激素诱导的特异性蛋白由两个亚单位组成,分子量分别为170和147kDa,该蛋白与成熟雌鱼所具有的特异性蛋白完全一致,而且分子量在其它硬骨鱼类卵黄蛋白原分子量范围之内,确定该蛋白为稀有(鲍)鲫的卵黄蛋白原

稀有（鱼句）鲫卵黄蛋白原电泳表征

鱼类卵黄蛋白原是检测环境内分泌干扰化合物雌激素效应的敏感生物标志物.稀有(鲍)鲫是我国目前最具发展潜力的用于生态毒理学研究的实验室模型物种.本文采用强效人工合成的雌激素乙炔基雌二醇对成熟雄性稀有(鲍)鲫和未成年稀有(鲍)鲫进行诱导,然后用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳对血清中的蛋白进行了表征,发现雌激素诱导的特异性蛋白由两个亚单位组成,分子量分别为170和147kDa,该蛋白与成熟雌鱼所具有的特异性蛋白完全一致,而且分子量在其它硬骨鱼类卵黄蛋白原分子量范围之内,确定该蛋白为稀有(鲍)鲫的卵黄蛋白原

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies