肿瘤转移检测标志基因研究进展

用聚合酶链反应 (PCR)方法检测肿瘤转移标志基因的研究报道逐年增多 ,PCR法检测循环肿瘤细胞和微转移 ,不仅可确认癌转移 ,而且对检测治疗的效果、强度和持续时间有指导意义 ,对肿瘤转移规律的研究也将有深远的影响

铁蛋白反应器储存有毒金属离子的初步研究

探讨铁蛋白反应器在模拟流动海水体系中储存有毒金属离子的能力及规律.在体外,脱铁核铁蛋白能重新构建新的铁核且核中多数铁组分经含有5% 氯化钠的海水处理后仍可稳定于蛋白壳中,只有少量对H+ 或OH- 较敏感的铁组分随反应体系的pH值增加或减少而被直接释放于介质中.此外,铁蛋白还能储存Co2+ 、Ni2+ 、Mn2+ 和Zn2+ 等有毒金属离子,并释放相对应的铁量,其储存离子量和释放Fe3+ 量的摩尔比为1:1,该储存量及能力与环境介质的pH值有关,与Na+ 、K+ 、Ca2+ 等非过渡金属离子无关.经改造后的铁蛋白反应器预计可用于监测流动水域的有毒金属离子

PURIFICATION of P49 ANTIGEN GENE PRODUCT of TRICHINELLA SPIRALLS EXPRESSED IN ESCHERICHIA COLI

目的建立旋毛虫P49抗原基因原校表达产物的纯化方法。方法菌体经冻融、超声破菌及提取包涵体后，用离子交换和凝胶过滤层析纯化蛋白质。结果纯化后的融合蛋白P49/gST经SdS-PAgE电冰鉴定达电泳纯。双抗体夹心ElISA法检测结果表明纯化的P49/gST融合蛋白能与旋毛虫感染的鼠血清和纯化融合蛋白免疫的兔血清反应，而不与正常民和兔血清反应。结论纯化后的融合蛋白P49/gST具有较高的纯度及较强的免疫活性，可望作为旅毛虫病的免疫诊断抗原。Aim To establish a purification procedure for p49 antigen gene product of Trichinella spiralis expressed inEschedchia coli.Methods After centrifugation of the bacterial culture, the bacterial pellet was resuspended and lysed byfreezing-thawing treatment and ultrasonication.Ion exchanged and gel filtration chromatography were used to purify the fu-sion protein p49/GST from the inclusion bodies- Re8ultS The purity of the fusion protein p49/GST was verified using SDS-PAGE.The purified p49/GST protein could react with mouse antisera against Tricinella sPiralis and rabbit antisera againstpurified p49/GST protein, but not with normal mouse and rabbit sera by sandwich EL1SA.Concluslon The purified fusionprotein p49/GST with high purity and good immune activity could be used for the immune aasay of trichinellosis.福建省重点（农医）项目!95－Z－15

银染巢式RT-PCR检测胃癌淋巴结微转移方法

以Ｋｅｒａｔｉｎ１９ｃＤＮＡ的套式引物建立了巢式ＲＴ－ＰＣＲ扩增Ｋｅｒａｔｉｎ１９ｍＲＮＡ的体系及ＰＡＧＥ－银染检测扩增产物的方法；对扩增体系进行优化后，分析了扩增特异性及检测敏感性，并对临床样品作了初步检测．结果表明：该扩增体系具有较好的特异性，其敏感性可达１０－６μｇＲＮＡ，即可从１０５个淋巴细胞中检出１个胃癌细胞；对临床样品检测的结果与病理检查结果相符．显示该法具有较好的可靠性

DETECTING GASTRIC CANCER MICROMETASTASES IN AXILLARY LYMPH NODES BY RT-PCR AMPLIFICATION AND SILVER STAINING

作者单位: 1 厦门大学生命科学学院抗癌中心( 厦门361005)，2 厦门大学生命科学学院生物系，3 厦门市中山医院[中文文摘]目的　建立RT PCR检测胃周淋巴结胃癌转移细胞的方法 ,评价其临床应用价值。方法　以MUC1cDNA的引物建立了RT PCR扩增体系及PAGE 银染检测PCR扩增产物的方法 ,并作了优化 ;在对扩增体系的检测特异性和敏感性作了分析后 ,初步检测了临床样品。结果　该扩增体系具有较好的特异性 ,敏感性可达 10 - 6 μgRNA ,相当于从 10 5个淋巴细胞中检出 1个胃癌细胞 ;对临床样品检测的结果与病理检查结果相符。结论　该法具有较好的可靠性 ,可作为临床常规病理探查的补充检查手段。[英文文摘]Objective To develop a RT- PCR method for detecting gastric cancer micrometastases in axillary lymph nodes. Methods T he RT- PCR was set up fo r amplifying MUC1 mRNA and the PAGE- silver staining for detecting amplification products. After optmizing t he PCR system, the spec-i ficity and sensitivity were investigated by amplifying different selected samples and ser ial dilution method respectively . This method was performed on axillary lymph nodes and compared t he results wit h that of patholo gical examination for estimating t he reliability. Results This system showed a good specificity and a high sensitivity of detecting one gastric cancer cell out of 105 lymphocytes. The r esults corresponded with the pathological examination suggesting a good reliability of this method. Conclusion The RT- PCR method possesses a high reliability and promise in clinical appilcation.福建省卫生厅基金!(96075); 厦门大学肿瘤细胞工程开放实验室基金资助项

2型糖尿病发病的遗传因素和环境因素(英文)

目的　探讨遗传因素与环境因素在 2型糖尿病病因组合中的相互关系以及各自所占的比重 ,为 2型糖尿病的综合预防和干预提供科学依据。方法　采用 1∶2配比的病例对照研究和家系调查的方法 ,选择济宁市新诊断 2型糖尿病人 5 6例、医院对照 112例 ,对研究因素进行单因素和多因素条件logistic回归分析并分析遗传方式和遗传率。结果　病例组一级亲属总的累积发病率为 3.96 3% ,与对照组 0 .6 2 5 %相比 ,差异有非常显著性 (P <0 .0 0 5 ) ;2型糖尿病的遗传度为 36 .2 % ;分离分析 2型糖尿病符合多基因遗传模式。Logistic回归分析表明肥胖、糖尿病家族史和嗜甜食与 2型糖尿病有显著性关系。结论　对于该病的防制对策应针对那些有遗传背景的人群 ,注意避免肥胖 ,少吃甜食 ,加强监测 ,尽早的发现潜在的或亚临床型的 2型糖尿病 ,以便及时治疗 ,减少糖尿病的危害有着重要意义

Study on Antitumor Activity of Tachypesin I against Human Promyelocytic Leukemia Cell Line HL 60 from Hemocyte of Horseshoe Crab

利用从中国鲎血细胞中提取的鲎素T-1作抗肿瘤活性实验.结果表明，鲎素T-1在体外对人早幼粒白血病Hl-60细胞的增殖有明显抑制作用.急性毒理实验显示，鲎素T-1对正常活体毒性较小，可用于活体实验.荷瘤裸鼠实验统计，鲎素T-1对肿瘤抑制率达42.3%.The antitumor activity of tachyplesin I(T 1) extracted from the hemocytes of horseshoe crab Tachypleus tridentatus for human promyelocytic leukemia cells line HL 60 has been studied.It was demonstrated that tachyplesin I had the remarkable inhibitory activity on proliferation of HL 60 cells in vitro experiment.The result from acute toxicological assay showed that tachyplesin I only had a little toxicity and could be used in intravital research.The rate of tumorous suppression of tachyplesin I reached to 42.3% on HL 60 cells.福建省自然科学基金;厦门市海洋科技计划资助项

Measurement of integrated luminosity of data collected at 3.773 GeV by BESIII from 2021 to 2024

We present a measurement of the integrated luminosity e+e- of collision data collected by the BESIII detector at the BEPCII collider at a center-of-mass energy of Ecm = 3.773 GeV. The integrated luminosities of the datasets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 were determined to be 4.995±0.019 fb-1, 8.157±0.031 fb-1, and 4.191±0.016 fb-1, respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects, and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements