一种利用荧光检测微囊藻细胞DNA损伤的方法

本发明公开一种利用荧光检测微囊藻细胞DNA损伤的方法，其步骤：（a）将待检测样品和对照样品均分别平均分成三小组；（b）去除细胞角质鞘：离心收集样品中藻细胞并重悬浮于SE缓冲液中洗涤；（c）细胞裂解：将细胞分别重悬浮于Lysis裂解液，加入蛋白酶K和十二烷基磺酸钠，使细胞裂解；（d）细胞DNA链解旋：改变pH，使T、P和B样品在不同条件下解旋；（e）染色：分别向以上T、P和B样品中加入Hoechest 33258染色；（f）荧光测定：离心后于荧光检测器中检测上清液的荧光强度；（g）结果计算：根据待测样品和对照样品中T、B和P样品的荧光来计算DNA链的断裂水平。本方法易于掌握，并且灵敏度高，DNA链上单个断裂位点即可检测到。</p

新社村与华人街——论《女勇士》的文本空间建构

在《女勇士》中,汤亭亭打破了惯常叙述时间的限制,把不同的生存体验共置于一个具有平面特性的文本空间中,构成了一种强烈的空间相互博弈的张力,也赋予了文本空间多重的内涵,从而表征了后现代语境中生存空间体验的多元化与丰富性。广西教育厅科研项目,项目编号:200705MS04

On H, D. Thoreau＇s Selection of Prose Style

梭罗选择散文作为其主要的创作形式是受到西方文体传统、19世纪美国历史文化语境及其个人创作取向、精神特质等方面影响的,这恰恰反映了梭罗的审美追求及其作品的文学史意义和艺术价值。H. D. Thoreau＇s selecting prose as his major writing style was af-fected by Western prose-writing tradition, American historical, cultural context in the 19th century and his creative orientation and personality trait, which precise-15, reflects Thoreau＇s aesthetic pursuit and the literature-history significance and artistic value of his works.2007年度广西教育厅科研项目“美国现代民族文化政策与美国少数族裔文学兴起”(项目编号:200705MS040

梭罗与康科德小镇

在整个美国文学界,或许没有一个作家与一个地方的联系比梭罗与康科德更紧密的。康科德独特的地理环境为梭罗的文学成长提供了厚重宽松且丰富多元的人文氛围。康科德的自然不仅是梭罗创作的对象也是其灵性得以滋养和不断更新的源泉

国外梭罗研究概述

梭罗:值得关注的美国经典作家亨利·戴维·梭罗(Henry David Thoreau,1817—1862),是目前国内最广为人知的美国19世纪作家之一。梭罗研究专家斯蒂芬·哈恩认为,只有爱伦·坡(Allan Poe)的名望和价值才能与之相比。著名小说家约翰·厄普代克(John Updike,1932—)在为新版的《瓦尔登湖》作序时指出,在19世纪美国文学经典作家如爱默生、霍桑、惠特曼、麦尔维尔等人之中,梭罗对美国的思想与文学贡献最大,是美国国民性格的杰出代表和源泉。：北京语言大学研究生创新基金项目（中央高 校基本科研业务费专项资金

关联理论与《哈克贝利·费恩历险记》中的反讽

以认知语用学中的关联理论为框架,以美国著名作家马克.吐温的代表作之一《哈克贝利.费恩历险记》为文本,从宏观(全文叙述的情节所体现的主旨)和微观(小说言语的词句细节)的角度对《哈克贝利.费恩历险记》的特色语言——反讽分析,结果表明关联理论运用于文学批评不仅可行,而且突破了结构语言学的框架,把语言学阐释和文化批评、心理学批评连接了起来,实现文学文本的"整体性"认知——既有文本内部的细致探究,又有外部历史文化背景的宏观考量,可谓内外兼顾。因此可以说,关联理论为文学批评提供了在认识论和方法论上创新的可能。Abstract: Taking cognitive pragma tics’ relevance theory as framework and The Adventures of Huckleberry Finn, the masterpiece of the famous American writer Mark Twain as version, this thesis aims to analyze its irony , the characteristic feature of the novel, in the ways of macro-angle ( subject reflected in the discourse) and micro-angle ( v erbal details of speech acts) . The present study shows that irony of various forms in literary works, just like that of “ ordinary language” , can be described within the framework of relevance theory. This also demonstrates that relevance theory employed into literary criticism can help to break th rough the structural linguistics framework and get the combination of linguistic criticism, cultural criticism and psychological criticism, thus to achieve “ overall” understanding: both detailed inquiry within the text, and external historical and cultural background beyond. The refore, it proves that the applicability of relevance theory to literary criticism may provide the possibility of innovation in epistemology and methodology

《老人与海》的生态批评解读

在《老人与海》中,人与自然、人与人之间的危机来源于人类自身的自负和悲剧思维,人与自然、人与人之间存在着一种相互关爱、相互依存的关系。正是这种关系,才能实现人类与大自然的"诗意栖居"

《老人与海》的悲剧性及其宗教仪式

通过分析海明威的经典著作《老人与海》中存在的悲剧性意味及其宗教仪式,发现主人公圣地亚哥是一个被神化的渔夫,这说明作品中悲剧性意蕴是一种叙述策略,目的在于凸显人的神话。北京语言大学青年自主科研支持计划资助项目(中央高校基本科研业务费专项资金资助项目

Liquid and solids charging : charge excess and surface properties effect

Orientador: Fernando GalembeckTese (doutorado) - Universidade Estadual de Campinas, Instituto de QuímicaResumo: Nesta tese, investigou-se a eletrização e os mecanismos de acúmulo de carga em gotas de água e filmes de polietileno, bem como, avaliou-se o efeito das cargas elétricas em algumas propriedades desses materiais. A eletrização de água foi realizada de duas formas: pelo contato com uma superfície metálica polarizada ou expondo as gotas de água a um potencial eletrostático criado através do ar por um eletrodo sem contato. Em ambos os casos, observou-se que a água adquire excesso de cargas e que o valor detectado excede o limite de Rayleigh em algumas dezenas. Verificou-se que a presença de cargas em gotas de água leva à diminuição de sua tensão superficial e ao aumento espontâneo de sua área, por outro lado, não foi observada mudança na sua densidade. O reconhecimento desses fatos deve contribuir para uma maior compreensão de vários fenômenos eletrostáticos que ocorrem na presença de água em estado líquido ou adsorvida. A superfície superior e inferior de filmes de polietileno expostos à descarga corona adquire potencias elevados e opostos formando um filme dipolar. No entanto, a molhabilidade, a composição química e a rugosidade das duas superfícies são bastante diferentes. Enquanto a superfície superior é modificada pelo tratamento corona, a superfície inferior, apesar de se tornar eletrizada, permanece inalterada após o tratamento. Estes resultados mostram que a eletrização da superfície voltada para o eletrodo corona ocorre por um processo diferente da superfície oposta. A eletrização da superfície inferior durante o processo corona abre possibilidades para aplicações onde seja desejável ter um filme eletrizado, porém sem modificar as características originais da superfícieAbstract: In this thesis, we investigated charging process and the mechanism of charge build-up in water droplets and polyethylene films as well. We evaluated the effect of electrical charges on some properties of these materials. Charging of water drops was done using two different methods by: contacting an electrified metal or exposing water to the electrostatic potential created through air by a noncontacting biased electrode. In both cases, water drops acquire net electric charge and its value exceeds the Rayleigh limit in a few tens. It was verified that the presence of charges in water droplets leads to reduced surface tension and increased spontaneous its area, but there was no change in density. Recognition of these facts should contribute to further understanding of various electrostatic phenomena taking place in the presence of liquid or adsorbed water. Top and bottom surfaces of polyethylene (PE) films exposed to corona discharge display large and opposite electrostatic potentials, forming an electric bilayer. However, water wetting, chemical composition and roughness of the two surfaces are quite different. While the top surface is modified by corona treatment, the bottom surface is charged but remains unchanged after treatment. These results show that poling the surface closer to the corona electrode triggers another but different charge build-up process at the opposite surface. Charging of the bottom surface during corona opens possibilities for applications where it is desirable to have a polymer film charged, but without modifying the original characteristics of the surfaceDoutoradoFísico-QuímicaDoutora em Ciência