低频脉冲超声对骨关节炎中软骨细胞的影响

超声被广泛应用于临床的诊断和治疗,但是临床上低频脉冲超声(lIPu)的应用却非常有限。在实验中,lIPu的应用却是很广泛。在许多的动物实验中证实,lIPu对许多结缔组织疾病有一定疗效。骨关节炎(OA)是以关节软骨细胞、细胞外基质、软骨下骨等合成与分解代谢失衡,关节软骨损坏为特征的全关节疾病。国家自然基金资助(81272168

脉冲电磁场对卵巢切除大鼠骨组织活化T细胞核因子2和空泡型V-ATP酶mRNA表达的影响

目的:探讨脉冲电磁场(pulsed electromagnetic fileds,PEMF)对卵巢切除(ovariectomized,OVX)大鼠骨组织细胞核因子κB受体活化因子(receptor activator of NF-κB,RANK),活化T细胞核因子2(nuclear factor of activated T2,NFAT2)和空泡型V-ATP酶(vavcuolar H+-ATPase,V-ATP)表达的影响。方法:将48只SD大鼠随机分为假手术组(SHAM),卵巢切除组(OVX),卵巢切除+脉冲电磁场治疗组(OVX+PEMF)。OVX+PEMF组大鼠在频率8Hz,磁场强度3.8m T的PEMF下每天干预40min,干预8周和16周后检测大鼠骨密度和骨组织RANK,NFAT2和V-ATP的mRNA表达水平。结果:OVX组BMD在第8周(P0.05,而SHAM组NFAT2与V-ATP表达均低于OVX组(P0.05;16周时OVX+PEMF组RANK和NFAT2表达均高于SHAM组(均P0.05。OVX组与OVX+PEMF组相比,8周时两组RANK和V-ATP表达均P>0.05,OVX+PEMF组NFAT2表达低于OVX组(P0.05,NFAT2和V-ATP在OVX+PEMF组的表达均下降(P<0.01,P<0.05)。结论:PEMF可通过下调OVX大鼠骨组织中NFAT2和V-ATPmRNA的表达从而抑制破骨细胞的骨吸收,进而延缓OVX大鼠的骨丢失。国家自然科学基金(81272168);;福建省自然科学基金(2016J01623,2017J01375);;福建省卫生计生委青年科研课题(2015-2-45

TNF-α及TNF-α抗体对破骨细胞V-ATP酶的影响

目的研究不同浓度的TNF-α及TNF-α抗体对破骨细胞上V-ATP酶表达量的影响。方法体外诱导小鼠RAW264.7细胞分化为破骨细胞,通过抗酒石酸酸性磷酸酶染色检测破骨细胞生成情况。然后将破骨细胞分为对照组、TNF-α干预组及TNF-α抗体干预组,TNF-α干预组、TNF-α抗体干预组分别用低、中、高三种浓度的TNF-α、TNF-α抗体干预48 h。用实时荧光定量聚合酶链反应(real-time PCR)、Western blot检测破骨细胞V-ATP酶的mRNA和蛋白表达水平。结果 TRAP染色检测提示有多核破骨细胞生成。TNF-α处理组V-ATP酶mRNA表达水平显著高于对照组(P<0.001);TNF-α抗体处理组V-ATP酶mRNA表达水平显著低于对照组(P<0.001)。同时,TNF-α处理组V-ATP酶蛋白表达水平显著高于对照组(P<0.05);TNF-α抗体处理组V-ATP酶蛋白表达水平显著低于对照组(P<0.05)。结论 TNF-α可提高破骨细胞V-ATP酶的表达;TNF-α抗体可抑制破骨细胞V-ATP酶的表达。上述提示TNF-α可能通过提高破骨细胞V-ATP酶的表达从而增加破骨细胞的骨吸收作用。国家自然科学基金资助项目(81272168);;福建省自然科学基金资助项目(2016J01623

东湖茶港排污口底泥对稀有鮈鲫的毒性

以东湖茶港排污口底泥复溶水为试验相,采用96h急性毒性试验和胚胎&mdash;卵黄囊吸收阶段毒性试验方法,研究了东湖茶港排污口底泥对稀有的鮈鲫毒性。结果显示,高浓度的复溶水对稀有鮈鲫胚胎、仔鱼和幼鱼具有明显的毒性效应,而胚胎&mdash;卵黄囊吸收阶段更为敏感。随着复溶水浓度的增加,稀有鮈鲫受精卵孵化率降低,仔鱼畸形率增高、成活率降低、生长减慢;对胚胎&mdash;卵黄囊吸收阶段的NOEC、LOEC和MATC分别为12.5%、25%和17.68%;对幼鱼96h LC50为69.1%。本文的研究还表明,底泥经晾晒后毒性大幅降低,暗示恢复东湖

Effects of Morinda officinalis-containing serum on the m RNA expression of C-FOS and Cbfa1 in osteoblast and osteoclast co-cultured system

目的观察不同浓度巴戟天含药血清对体外培养成骨-破骨细胞共育体系中原癌基因(C-fOS)、核心结合因子(CbfA)1 MrnA表达的影响。方法提取24 H内新生Sd乳鼠颅盖骨分离培养成骨细胞,采用5周龄Sd大鼠双侧股骨、胫骨的骨髓基质细胞,加入集落细胞刺激因子(M-CSf)和细胞核因子κb受体活化因子配体(rAnkl)诱导培养破骨细胞。采用碱性磷酸酶(AlP)染色鉴定成骨细胞,抗酒石酸酸性磷酸酶(TrAP)染色、骨吸收陷窝甲苯胺蓝染色、电镜扫描等鉴定破骨细胞,体外建立成骨-破骨细胞共育体系,设置低、中、高三种浓度巴戟天含药血清组和不含药血清组,干预3 d后提取各组总rnA,应用逆转录-聚合酶链反应(rT-PCr)方法测定各组C-fOS、CbfA1 MrnA表达量。结果不同浓度巴戟天含药大鼠血清对成骨-破骨细胞共育体系C-fOS有抑制作用,对CbfA1 MrnA的表达有促进作用。高浓度含药血清组两者的表达差异显著(P<0.05,P<0.000 1)。结论巴戟天含药血清可抑制成骨-破骨细胞共育体系C-fOS的表达,促进CbfA1 MrnA的表达,从而达到降低破骨细胞分化成熟及骨吸收活性,促进骨形成。Objective To observe the effects of the serum of Morinda officinalis( RMO) on the expression of C-FOS and core binding factor Alpha1( Cbfa1) in osteoblast and osteoclast co-cultured system.Methods Osteoblasts were separated from the cranium of 24 hours newborn SD rat.Bone marrow cells were harvested from bilateral femora and tibiae of five weeks old SD rat,and M-CSF and RANKL were used to induce osteoclast formation.Osteoblast cells were confirmed by alkaline phosphatase( ALP) stain,osteoclast cells were confirmed by tartrate resistant acidphos phatase( TRAP) stain,Toluidine blue stain and bone resorption assay.Osteoblast and osteoclast co-cultured system was established in vitro.Low,middle,high concentrations of serum RMO and control groups were set.Total RNA was extracted after intervention 3 days,C-FOS and Cbfa1 mRNA expression were measured by real-time PCR.Results Different concentrations serum of RMO had inhibitory effect on the expression of C-FOS and enhance the mRNA expression of Cbfa1 mRNA,and the function on both indicated a statistically significant difference at high concentration( P<0.05,P<0.000 1).Conclusions The serum of RMO could down-regulate the expression of C-FOS and up-regulate Cbfa1 mRNA in osteoblast and osteoclast co-cultured system,consequently reduce osteoclast differentiation and activity of bone resorption,enhance bone formation.国家自然科学基金资助项目(No.81272168); 福建省医学创新课题资助项目(No.2012-CXB-32

The Electrochemical Behavior and Determination of GSH at the Powder Microelectrode

　研究GSH在铂、玻碳以及用乙炔黑填充的粉末微电极(AB PME)表面的电化学行为.结果表明,在铂和玻碳电极上,GSH的电化学氧化是一个高度不可逆的反应,相应的CV曲线均未出现氧化电流峰或电流平台.而在AB PME电极上,GSH电化学氧化的表观可逆性得到明显提高,其超电势较常规电极下降约0.5V.且其氧化电流峰,与溶液本体的GSH浓度成良好线性关系.采用此法可完全消除AA和UA对GSH测定的干扰,由"内标法"成功地检测了人体血液中的GSH的浓度.又根据稳态极化曲线和极限扩散电流测定,GSH在AB PME电极表面的电化学氧化是一个单电子反应.In this paper, the electrochemical behavior of GSH at the surface of platinum(Pt) electrode, glass carbon(GC) electrode and acetylene black packed powder microelectrode (AB-PME) was studied. It was found that the electrochemical oxidations of GSH at both Pt and GC electrode were highly irreversible. No current peak or current plateau could be observed from the CV curves. The direct electro-oxidation of GSH at carbon surface was achieved by using the PME technique.In this case, the apparent reversibility of GSH oxidation was enhanced significatly .The overpotential of GSH oxidation was reduced to 0.5 V at the surface of AB-PME compared with that obtained with Pt and GC electrodes. The exhaustive electrolysis of GSH within the micro-cavity of the PME can be achieved. As a result, the peak currents obtained with AB-PME were proportional to GSH concentration. The interference caused by ascorbic acid and uric acid was eliminated completely by employing AB-PME electrode. The concentration of GSH in human erythrocyte was determined by employing the AB-PME technique and using the method of ”internal standard addition”. The steady state polarization curve of GSH was obtained with the AB-PME electrode .The number involved in the electrochemical oxidation of GSH was calculated as 1 by using the value of I_(L).作者联系地址：武汉大学化学与分子科学学院电化学研究中心,武汉大学化学与分子科学学院电化学研究中心,武汉大学化学与分子科学学院电化学研究中心,武汉大学化学与分子科学学院电化学研究中心 武汉430072 ,武汉430072 ,武汉430072 ,武汉430072Author's Address: College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072,Chin

Measurement of integrated luminosity of data collected at 3.773 GeV by BESIII from 2021 to 2024

We present a measurement of the integrated luminosity e+e- of collision data collected by the BESIII detector at the BEPCII collider at a center-of-mass energy of Ecm = 3.773 GeV. The integrated luminosities of the datasets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 were determined to be 4.995±0.019 fb-1, 8.157±0.031 fb-1, and 4.191±0.016 fb-1, respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects, and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements