应用CSC-GE-80基因芯片筛查人子宫内膜早期异位种植的血管生长相关基因

【目的】探讨子宫内膜异位早期种植的相关基因,特别是与早期种植相关的血管生长基因。【方法】采用裸鼠人子宫内膜皮下种植的方法,建立裸鼠人子宫内膜异位症模型,在种植后14d,取出成模病灶与未种植内膜进行CSC-GE-80基因芯片筛查比较。【结果】在基因表达谱中最有显著性上调基因50个,最有显著性下调基因47个,包括生长因子和细胞因子及其受体的变化,其中生长因子受体结合蛋白10（growth factor receptor-bound protein10,Grb10）与丁酸盐反应因子1（butyrate response factor 1）中表皮生长因子（EGF-response factor 1）上调明显,下调明显胰岛素样生长因子结合蛋白7（insulin-like growth factor binding protein 7）。【结论】通过基因筛查我们初步考虑生长因子结合蛋白10（growth factor receptor-bound protein 10）有可能参与早期异位内膜的血管生长

Physical health care monitoring for people with serious mental illness

Background Current guidance suggests that we should monitor the physical health of people with serious mental illness and there has been a significant financial investment over recent years to provide this. Objectives To assess the effectiveness of physical health monitoring as a means of reducing morbidity, mortality and reduction in quality of life in people with serious mental illness. Search methods We searched the Cochrane Schizophrenia Group Trials Register (October 2009) which is based on regular searches of CINAHL, EMBASE, MEDLINE and PsycINFO. Selection criteria All randomised or quasi-randomised clinical trials focusing on physical health monitoring versus standard care or comparing i) self monitoring vs monitoring by health care professional; ii) simple vs complex monitoring; iii) specific vs non-specific checks iv) once only vs regular checks or v) comparison of different guidance. Data collection and analysis The authors (GT, AC, SM) independently screened search results and identified three studies as possibly fulfilling the review's criteria. On examination, however, all three were subsequently excluded. Main results We did not identify any randomised trials which assessed the effectiveness of physical health monitoring in people with serious mental illness. Authors' conclusions There is no evidence from randomised trials to support current guidance and practice. Guidance and practice are based on expert consensus, clinical experience and good intentions rather than high quality evidenc

血浆ADAMTS-13活性和NLR在急性肺血栓栓塞症中的诊断价值

目的探究急性肺血栓栓塞症（PTE）患者血浆血管性血友病因子（vWF）活性、血管性血友病因子裂解酶（ADAMTS-13）活性、中性粒细胞计数与淋巴细胞计数比率（NLR）健康对照组的表达差异，探究ADAMTS-13活性、NLR对于PTE患者的诊断意义。方法共纳入急性PTE患者64例及健康对照者30例，收集相关临床数据并采用酶联免疫吸附试验（ELISA）检测血浆ADAMTS-13活性、vWF活性，分析比较ADAMTS-13活性、vWF活性的组间差异及其与其他临床指标的相关性，绘制受试者工作特征曲线（ROC）评价ADAMTS-13活性及NLR对急性PTE患者的诊断价值。结果健康对照组与急性PTE组ADAMTS-13活性水平分别为83.8%（72.2%， 98.8%）、63.3%（49.6%， 74.8%），vWF活性水平分别为102.7%（89.7%， 117.2%）、122.6%（96.8%， 156.6%），组间差异均具有统计学意义（P＜0.05），ADAMTS-13活性与vWF活性、D-二聚体水平呈负线性相关（P＜0.05）。多因素Logistic回归结果示NLR的升高为患急性PTE的独立危险因素［OR=1.66，95% CI（1.10， 2.47），P=0.014］，ADAMTS-13活性的升高为患急性PTE的独立保护因素［OR=0.92，95% CI（0.88， 0.96），P＜0.001］。ADAMTS-13活性用于诊断急性PTE时，ROC曲线下面积（AUC）为0.861，95%CI（0.780， 0.942），P＜0.001，联合NLR用于诊断急性PTE时，AUC为0.902，95%CI（0.841， 0.963），P＜0.001。结论急性PTE患者血浆ADAMTS-13活性显著下降，vWF活性显著上升，ADAMTS-13、vWF或为沟通血栓形成与炎症反应间的桥梁，血浆ADAMTS-13活性联合NLR可提高诊断急性PTE的准确性，为探寻急性PTE的致病机制提供新的思路，为探寻急性PTE治疗靶点提供实验依据

PCNA 表达水平对上皮性卵巢癌化疗效果的影响

【目的】探讨增殖细胞核抗原(PCNA)表达水平对上皮性卵巢癌化疗效果的影响。【方法】应用PCNA 单克隆抗 体检测81 例上皮性卵巢癌的表达水平, 术后用以铂类为基础的方案进行化疗, 并分析她们的2 次探查结果及生存率。【结果】 PCNA 总的阳性表达率为80 %(65/ 81), 按增殖指数水平分A 、B 、C 3 组, 2 次探查结果A 组阳性率为44.18 %, B 组20.69%, C 组11.11%,(P <0.05)。A 组3 年及5 年生存率是59.68 %和45.60%, B 组55.70 %和45.57 %, C 组74.66 %和74.66 %。 Kaplan-Meier 生存曲线无差异(Log-rank 检验P >0.05)。【结论】PCNA 表达对上皮性卵巢癌化疗效果近期有影响, 随PCNA 增殖指数升高, 第二次手术探查阳性率下降。对化疗的远期效果——— 生存时间影响不大

血管内皮生长因子C及膜突蛋白促宫颈癌恶性进展的作用研究

【目的】 研究宫颈癌恶变过程中血管内皮细胞生长因子C （vascular endothelial growth factor C, VEGF-C）及膜突蛋白 （moesin）的表达变化，并在培养的宫颈癌细胞中初步探讨VEGF-C对膜突蛋白的调控作用。【方法】利用临床上活体取材的不同临床分期的宫颈癌组织标本及正常对照宫颈组织，蛋白免疫印迹法检测VEGF-C及moesin蛋白的表达情况。体外培养宫颈癌细胞株Hela细胞，蛋白免疫印迹法测定VEGF-C对moesin蛋白的表达和磷酸化的调控作用。【结果】 随着宫颈癌临床分期的增高，VEGF-C、moesin蛋白及磷酸化moesin蛋白表达水平均随之增加。与正常宫颈组织比较，宫颈原位癌（CIN）组织中VEGF-C、moesin、磷酸化moesin蛋白表达分别增加了（45±9）％、（63±12）％、（74±16）％（P < 0.05）；宫颈鳞癌I期各蛋白增高幅度分别为（94±18）％、（104±27）％、（123±30）％（P< 0.01）；宫颈鳞癌Ⅱ期（未放疗化疗）各蛋白表达增高更为显著，其幅度分别为（186±24）％、（246±37）％、（194±28）％（P < 0.001）。在培养的宫颈癌细胞株HeLa细胞上，VEGF-C（100 ?g/L）处理24 h后，可显著增高moesin蛋白、磷酸化moesin蛋白的表达。VEGF-C单克隆抗体可抑制VEGF-C对moesin蛋白表达及磷酸化的上调作用。【结论】 VEGF-C、moesin蛋白表达与宫颈癌恶性程度密切相关，VEGF-C可能通过上调moesin表达及其磷酸化水平而促进宫颈癌的恶性进展

一种碳纤维复合材料界面层的制备方法

本发明提供了一种碳纤维复合材料界面层的制备方法。该方法采用包括碳纳米管、酚醛树脂、固化剂与溶剂的上浆剂，将该上浆剂上浆至碳纤维表面，固化后进行高温热处理，得到包含碳纳米管的热解碳涂层，能在碳纤维为增强体的复合材料中起到界面增强增韧的效果。该方法简单易行，成本低，与现有的CVD与EPD相比工艺复杂度大大降低，并且工艺条件易于控制，制备过程中对碳纤维本身不产生损伤，能够降低对碳纤维本身性能的影响

大熊猫医疗行为训练

2011～2012年,依照大熊猫的特殊习性和能力设计训练项目,采取食物鼓励的正强化训练方法,配以口令和手势等指令持续对广州动物园保育的2只圈养大熊猫进行医疗管理训练,相继完成了站、坐、趴、卧等基础动作训练,并在此基础上完成了串笼、体尺体重测量、体温测量、肌肉注射、腕部采血、口腔及牙齿检查等训练项目。大熊猫的医疗管理训练的实施,减少了日常管理中动物对环境改变和人类干扰的应激反应,增加了人与动物的安全;以往需要麻醉动物才能进行的肌肉注射、采血、口腔及牙齿检查、体温测量等操作,现在可以引导动物在放松状态下主动完成,避免了麻醉对动物造成的伤害。圈养野生动物医疗行为训练不同驯兽表演,是提高动物福利的有效手段

General physical health advice for people with serious mental illness

There is currently much focus on provision of general physical health advice to people with serious mental illness and there has been increasing pressure for services to take responsibility for providing this. For the comparison of physical healthcare advice versus standard care we identified five studies (total n = 884) of limited quality. For measures of quality of life one trial found no difference (n = 54, 1 RCT, MD Lehman scale 0.00 CI -0.67 to 0.67) but another did (n = 407, 1 RCT, MD Quality of Life Medical Outcomes Scale - mental component 3.7 CI 1.7 to 5.6). There was no difference between groups for the outcome of death (n = 407, 1 RCT, RR 1.3 CI 0.3 to 6.0), for the outcome of uptake of ill-health prevention services, one study found percentages significantly greater in the advice group (n = 363, 1 RCT, MD 36.9 CI 33.1 to 40.7). Economic data were equivocal. Attrition was large (> 30%) but similar for both groups (n = 884, 5 RCTs, RR 1.18 CI 0.97 to 1.43). Comparisons of one type of physical healthcare advice with another were grossly underpowered and equivocal