The Morphology, Network and Ultrastructure Changes of the Interstitial Cells of Cajal in the Bladder of Guinea Pig with Diabetic Cystopathy

目的：建立糖尿病豚鼠膀胱功能失代偿模型，观察糖尿病膀胱病（DCP）豚鼠膀胱Cajal样间质细胞（ICCs）形态、网络结构和超微结构变化，探讨ICCs在DCP发病机制中的作用。 方法：50只英国种短毛雌性豚鼠，随机选择42只作为实验组，8只作为对照组。实验组单次腹腔注射链脲佐菌素（STZ），对照组注射相应剂量的枸橼酸缓冲液。4周后，两组豚鼠称重及测定餐后2小时血糖。符合糖尿病的豚鼠及对照组豚鼠，继续饲养5周和8周，尿动力学检测残余尿、排尿期最大逼尿肌压、膀胱容量及顺应性，确定膀胱功能代偿及失代偿(即DCP)糖尿病豚鼠，利用免疫组织化学荧光染色、激光共聚焦显微镜、透射电镜技术检测膀胱ICCs形态...Objective： Guinea pigs were used to establish a diabetic cystopathy（DCP） model, We observe the morphology, network and ultrastructure changes of the interstitial cells of Cajal (ICCs) in the bladders of guinea pigs with DCP, to discuss the functions of the ICCs in the the pathogenesis of the DCP. Methods： 50 short hair Britain female guinea pigs were divided into two groups randomly, 42 as the ex...学位：医学硕士院系专业：医学院_外科学学号：2452010115329

卤原子取代苯并噻二唑聚合物给体材料的合成及其光伏性能研究

通过对苯并噻二唑单元进行氟、氯等卤原子取代,并同时调节烷基侧链的长度,设计合成了一系列基于苯并噻二唑四噻吩类的聚合物太阳电池材料.不同卤原子取代以及烷基侧链的长度都会影响聚合物的结晶性和薄膜聚集形貌从而改变其带隙和电荷传输性质.氟、氯原子的引入可调节聚合物的能级结构,而且相对于氟原子而言,具有更大原子半径的氯原子的引入可在更大尺度下调节能级结构,从而大幅提高相应太阳电池的开路电压,同时通过侧链的优化可进一步调节聚合物的微观聚集结构,改善器件能量转换效率.结果表明,在氯原子和氟原子共同作用的情况下,引入较长的侧链有利于提升聚合物的开路电压和短路电流,从而获得较好的器件性能.其中,以氯、氟共同取代的聚合物PCFBT4T-2OD与PC71BM为活性层的器件性能最佳,能量转换效率可达8.84%.国家自然科学基金(基金号51773087,21733005);;广东省自然科学基金(基金号2016A030313637);;深圳市孔雀团队(项目号KQTD20140630110339343)资助项

Production of β-glucanase by cultivation of immobilized recombinant E.coli cells in a packed bed reactor

以多孔陶瓷为载体吸附法固定重组大肠杆菌E.coli JM 109-pLF3表达胞外β-葡聚糖酶。考察了固定床间歇培养时循环流速和曝气量对发酵液酶活力的影响。当循环流速达到44.19mL/min,曝气量达到0.6mL/min时,培养48h后,发酵液的酶活力达100.3U/mL。固定化细胞具有良好的重复使用能力,在连续5批次实验中,培养48h后的酶活力均在100U/mL左右。固定床连续培养时,固定化细胞能够保持恒定的产酶效率,当稀释率为0.05h-1时,发酵液中得到的酶活力为39.1U/mL。β-glucanase produced by immobilized Escherichia coli JM 109-pLF3 in a packed bed bioreactor was studied.In batch fermentation,the highest enzyme activity of 100.3 U/mL was achieved at a recycling rate of 44.49 mL/min and an aeration rate of 0.6 mL/min after 48 h cultivation.In repeated batch operation,the enzyme activity up to 100 U/mL was obtained in 5 cycless,howing high stability of the immobilized cells.In continuous fermentation,the production of β-glucanase by immobilized cells was kept more or less constant.When the dilution rate was 0.05 h-1,the enzyme activity in the fermentation broth was 39.1 U/mL.厦门市科技计划项目(No.3502Z20055017

人脐血单个核细胞移植对大鼠急性心肌梗死后血管再生的作用

[目的]探讨人脐血单个核细胞移植对急性心肌梗死后血管再生的作用.[方法]雄性Wistar大鼠60只随机分为对照组（心肌梗死）和移植组（心肌梗死＋细胞移植）各30只.结扎冠状动脉左前降支制作大鼠急性心肌梗死模型,以羟乙基淀粉沉淀加密度梯度离心的方法制备人脐血单个核细胞,并以5-溴脱氧尿核苷（BrdU）标记细胞.移植组大鼠模型制作成功后即在梗死区周边注射经分离并标记的人脐血单个核细胞混悬液（2×10^5/μL）,在对照组相同位置注入等量达尔伯克必需培养基（DMEM）.移植4周后用左心导管检测血流动力学改变,并取心脏组织行抗第Ⅷ因子（vWF）和BrdU免疫组化染色,观察毛细血管密度、移植细胞成活情况.并分别在移植后第4天、第7天、第14天、第28天取梗死周边区组织作血管内皮生长因子（VEGF）RT-PCR的半定量研究.[结果]移植组移植的单个核细胞可在梗死心肌内存活.移植组比对照组心功能明显改善,左心室舒张末压（LVEDP）明显降低（21.08±8.10）mmHg vs（30.82±9.59）mmHg,P＜0.05;左心室内压力最大上升速率（＋dp/dtmax）明显增快（4.29±1.27）mmHg/ms vs（3.24±0.75）mmHg/ms,P＜0.05;最大下降速率（-dp/dtmax）亦显著提高（3.71±0.79）mmHg/ms vs（3.00±0.49）mmHg/ms,P＜0.05.移植组梗死区周边毛细血管数显著增加（5.7±0.3）/HP vs（2.3±0.4）/HP,P＜0.01.VEGF mRNA表达在移植组于第7天明显增加,并持续至第28天,对照组于第4天及第7天可见少量表达,第14天与第28天明显减弱,两组差异有统计学意义（P＜0.01）.[结论]人脐血干细胞在未使用免疫抑制剂的条件下可成功移植到大鼠急性心肌梗死区,改善急性心肌梗死大鼠心功能,促进新生血管形成

Improving the Expression Level of Soluble Human Fas Ligand by Cultivating Recombinant Dictyostelium discoideum in CMC-ALG Microcapsules

首先研究了应用于液芯羧甲基纤维素钠-海藻酸钙(CMC-ALG)微胶囊制备中的各种化学组分对重组盘基网柄菌生长的影响,然后考察不同组分浓度制备而成的各种CMC-ALG微胶囊内重组盘基网柄菌的生长情况,从而得到较适的微胶囊制备的组分配比(CMC12g·L-1,SA8g·L-1,CaCl2100g·L-1)。结果表明,在以上较适条件下制备的微胶囊内重组盘基网柄菌的生长得到了极大的改善,最大的细胞密度比游离培养时提高了4倍,达到了8.0×107mL-1;相应的人类可溶性Fas配体(FasL)的表达水平也提高了1.5倍,达到了315μg·L-1。最后,开展了微胶囊化重组盘基网柄菌的二次重复发酵FasL的研究,结果表明,经过二次重复批次培养,最大细胞密度可达到1.24×108mL-1,为游离培养的8～10倍,而且FasL的表达水平还能维持高水平(280μg·L-1),为游离培养时的2倍。The biocompatibility between the growth of Dictyostelium discoideum AX3-pLu8 and chemical components used for the preparation of carboxy methyl cellulose-alginate(CMC-ALG)microcapsules was evaluated at first,and then the effects of the concentrations of each component forming the capsule on the growth behaviors of D.discoideum cultivated in it were studied.For microencapsulated cultivating D.discoideum,it was found that the most suitable capsule components are 12 g?L?1 of CMC,8 g?L?1 of sodium alginate(SA)and 100 g?L?1 of CaCl2.Using the microcapsules with mentioned composition,the cell density in it can reach 8.0×107 mL?1,this value is about 5 times that could be observed in suspension culture,and correspondently,in the capsules a high concentration(315 μg?L?1)of soluble human Fas ligand(shFasL)was detected,which is about 2.5 times higher than that obtained in suspension culture under the same culture condition.In addition,the immobilized cells could be used effectively for repeated batch cultivation and in the second repeated batch cultivation,a very high cell density(up to 1.24×108 mL?1)and still a high expression of FasL(280 μg?L?1)could be obtained.国家自然科学基金资助(20306025,30370039

Polyphenol Contents in Eight Fruits and Their Antioxidant Activities

[中文摘要]测定了苹果、石榴、橄榄、芒果、香蕉、菠萝、葡萄和龙眼的壳、肉及核中的多酚含量及其抗氧化性。以70%丙酮(v/v)为提取溶剂,室温下超声波辅助浸提样品后得到提取液。采用普鲁士兰(Prussian blue)法测定了提取液中多酚和单宁的含量,利用FRAP法测定其抗氧化性。结果表明:提取液中多酚和单宁的含量均与其抗氧化性成正相关关系;石榴、橄榄、芒果、葡萄和龙眼等水果的壳与核有望成为天然抗氧化剂的新来源。[英文摘要]The contents o f polyphenols of peel pulp and seed fractions of apple, pome granate, olive, mango, banana, pineapple, grape and long an and their to talantioxidant ac tiv ities were studied. A fter ultrasound-assisted extraction w ith 70% aqueous acetone at room temperature, polypheno ls and tann ins in the p lant ce lls w ere separated and their contents were determ ined by Prussian blue assay. Antioxidant activities of the aqueous acetone extracts were evaluated by Ferric Reducing /Antioxidant Power (FRAP) assay. A positive linear correlation between antioxidant activities and phenolic contents was observed. The resu lts show ed that the fru it pee l and seeds o f pom egranate, olive, mango, grape and longan could be conside red as potentia l sources o f antioxidants rather than just discarded as waste

改良分子信标-双重实时荧光PCR快速检测SARS病毒

目的建立改良分子信标-双重实时荧光PCR检测SARS病毒的方法,用于SARS的早期诊断和动物溯源。方法利用改良分子信标技术、装甲RNA和双片段双色荧光技术,根据GenBank公布的SARS病毒聚合酶基因1b的阅读开放框架结构的保守序列,自行设计一对引物和探针,以部分临床标本的酶联吸附实验结果和传统细胞培养方法作为对照,建立分子信标检测SARS病毒的方法。对368份临床标本(咽漱液、血液、粪便、尿液)、52份细胞培养液和50份动物标本进行荧光PCR扩增。结果分子信标检测SARS病毒的方法灵敏度为10～100个拷贝ml,与流感病毒等呼吸道病毒无交叉反应。分子信标检测368份临床标本,20份阳性。其中确诊病例阳性率为21.27%(1047),确诊病例的咽漱液阳性率为43.48%,还分别从粪便和血清中检测到SARS病毒。52份细胞培养液,29份阳性,阳性率为55.77%。50份动物标本,23份阳性,阳性率为46%。结论改良分子信标-双重实时荧光PCR检测SARS病毒方法灵敏度高、特异性强,可用于SARS的临床早期诊断和动物溯源

闽南文化研究国际笔谈会论点选载

2013年12月21日,由闽南师范大学闽南文化研究院主办的“2013闽南文化研究国际笔谈会“在国际学术交流中心召开。来自海峡两岸及日本、新加坡等国家的闽南文化研究方面的专家、学者二十余人出席了会议。会议围绕着闽南文化的内涵、外延及特征,闽南文化的当代价值与社会功能,闽南文化研究的理论与方法,闽南文化的跨文化阐释,闽南文化的世界性及其意义,闽南文化与两岸交流,闽南文化的生态保护,闽南文化学科建设与人才培养等议题展开了深入的研讨,言简意赅,探幽发微,对当下闽南文化理论研究势必产生积极的影响。本期节录专家们的精彩发言,以飨读者,也希望由此来带动和促进闽南文化研究的进一步升华

Combustion Synthesis and Electrochemical Properties of the Small Hydrofullerene C50H10

通讯作者地址: Xie, SY (通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China 3. Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R China 电子邮件地址: [email protected]; [email protected] hydrofullerene C50H10 is synthesized by low-pressure benzeneoxygen diffusion combustion. The structure of C50H10 is identified through NMR, mass spectrometry, and IR and Raman spectroscopy as a D5h symmetric closed-cage molecule with five pairs of fused pentagons stabilized by ten hydrogen atoms. UV/Vis and fluorescence spectrometric analyses disclose its optical properties as comparable with those of its chloride cousin (C50Cl10). Cyclic and square-wave voltammograms reveal that the first reduction potential of C50H10 is more negative than that of C50Cl10 as well as C60, with implications for the utilization of C50H10 as a promising electron acceptor for photovoltaic applications.973 projects 2011CB935901 NSFC 21031004 21021061 2077310

Simple Combustion Production and Characterization of Octahydro[60]fullerene with a Non-IPR C-60 Cage

1. Xiamen Univ, State Key Lab Phys Chem Solid Surface, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 3. Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R ChinaFor the first time an easier, operable combustion method is employed for the synthesis of non-IPR fullerene, and an octahydro[60)fullerene with a non-IPA C-60 cage (C-60 isomer C-#1809(60)) produced by combustion is isolated and characterized by MS, UV vis, IR, and NMR spectroscopies in combination with DFT calculations. This finding shows that, in addition to chlorine, hydrogen can be an ample cataloreactant for the production of non-IPR fullerene derivatives under such conditions as arc-burning and diffusion combustion.NSFC 20525103 20673088 20973137 20721001 20423002 21031004 973 projects 2007CB815301 2007CB81530