一种快速、无损大豆种子DNA提取方法的建立和应用

基因分型是进行植物基因功能的遗传分析和分子标记辅助育种的重要环节。该研究以大豆(Glycine max)成熟种子为材料, 建立了通过钻孔采集样品、快速提取DNA进行基因型鉴定的方法。用此方法, 一个熟练的工作人员可以在1个小时内完成120个样品的采集和DNA提取; 同时种子钻孔取样后, 不会对大豆种子的萌发造成影响。利用该方法获得的DNA可满足PCR扩增的要求。实验重复性好, 成功率在98%以上。这种快速且无损的大豆种子基因型鉴定方法可以用于鉴定杂交种子、品种纯度以及遗传分析等研究工作

植物对铁元素吸收的分子调控机制研究进展

铁是植物正常生命活动所必需的微量矿质元素, 在光合作用、呼吸作用等诸多生理代谢过程中发挥着重要的作用。虽然铁在地壳中含量丰富, 但因其在大多数类型土壤中利用率低, 导致植物缺铁现象比较普遍。植物在长期的进化过程中,演化出一套完整的铁吸收、运输和分子调控的系统。近年来, 国际上在揭示植物铁吸收、运输和储存的机制方面取得很大研究进展, 本文主要就植物对铁元素吸收分子调控机制的研究进展进行了综述, 以期为全面了解植物铁代谢的分子机制进行作物分子设计育种提供帮助

Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development

Soybean (Glycine max Merr.) is the world's most widely grown legume and provides an important source of protein and oil. Improvement of seed quality requires deep insights into the genetic regulation of seed development. The endosperm serves as a temporary source of nutrients that are transported from maternal to filial tissues, and it also generates signals for proper embryo formation. Endosperm cell death is associated with the processes of nutrient transfer and embryo expansion. The bHLH domain transcription factor AtZHOUPI (AtZOU) plays a key role in both the lysis of the transient endosperm and the formation of embryo cuticle in Arabidopsis thaliana. There are two copies of soybean GmZOU (GmZOU-1 and GmZOU-2), which fall into the same phylogenetic clade as AtZOU. These two copies share the same transcription orientation and are the result of tandem duplication. The expression of GmZOUs is limited to the endosperm, where it peaks during the heart embryo stage. When the exogenous GmZOU-1 and GmZOU-2 were expressed in the zou-4 mutant of Arabidopsis, only GmZOU-1 partially complemented the zou mutant phenotype, as indicated by endosperm breakdown and embryo cuticle formation in the transgenic lines. This research confirmed that the GmZOU-1 is a ZOU ortholog that may be responsible for endosperm breakdown and embryo cuticle formation in soybean