Theoretical Research on Responsibility to Protect in International Law

本文着眼于国际法热点问题“保护责任”的相关理论与实践的研究，并尝试用法理学的相关学说来阐释这个国际法问题的理论内涵。本文共分四大部分，即国际法上保护责任的含义及其与人道干涉的异同，保护责任的现状与问题，保护责任的道德考量、法律分析与政治解读，以及保护责任的理论与实践前景和我国应坚持的立场。 在第一部分中，笔者对干涉与国家主权国际委员会所提出的“保护责任”这一概念做了全方位的介绍，这部分介绍主要基于该委员会在２００１年发布的报告。同时，笔者还分析了保护责任与人道干涉的异同，指出保护责任有其值得肯定的进步意义，其内涵比人道干涉更为广泛，但笔者也指出，对于绕过安理会使用武力干涉他国情势这一问题，保...This dissertation is focused on the relevant theories and practical issues of responsibility to protect which is almost the hottest international problem today. The author is also trying to interpret this question by employing jurisprudence views. This article is composed of four parts. First, the meaning of responsibility to protect is introduced based on the Report of the International Commiss...学位：法学硕士院系专业：法学院法律系_法学理论学号：1292007115018

Design and Implementation of Functions Recognition and Its Visualization System

本研究课题属于软件开发领域，开发基于Web的函数图像绘制软件。目前， 国内外对于函数图像绘制主要是使用MATLAB、Mathematica等软件实现，这些 软件功能相当强大，是数值计算最常用的工具软件。在使用它们时，用户需要学 习编程的方法。同时，用户购买它后，需要安装在自己的计算机上。以上情形是 最普遍的。MATLAB等软件工具可作为数值计算、函数图像绘制等需求的专用 软件，它们比VisualStudio等通用软件开发工具在使用上简单了许多，对用户编 程能力要求很低，但能更大化的方便用户使用，用更高级的语言形式实现人机交 互，有利于降低编程的困难。再者，一个软件对用户来说，其价...This research project belongs to the field of software developing, based on the development of Web image rendering software function. At present, the main function is to image rendering using MATLAB, MathCAD etc software to realize, the software function quite powerful, is the most commonly used numerical calculation software tools. In when using them, users need to learn programming method. A...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X200923007

儿童遗传性心律失常的诊治进展

回顾在遗传性心律失常领域最新发表的相关研究,主要关注与儿童心源性猝死关系密切的离子通道病,包括长QT综合征(LQTS)、短QT综合征(SQTS)、Brugada综合征(BrS)和儿茶酚胺敏感性多形性室性心动过速(CPVT),总结它们在发病机制及诊治方面的进展

基于电子鼻的慢性胃炎气滞证患者常见病位的口腔呼气气味图谱研究

目的:运用电子鼻探讨慢性胃炎气滞证患者常见病位的口腔呼气气味图谱特征。方法:采用证素辨证的方法,筛选出397例慢性胃炎气滞证患者并判断病位证素,同时运用基于阵列式气体传感器技术的医用电子鼻(EN0l1103-A)采集其口腔呼气的气味图谱,选择气味图谱响应曲线的振幅、斜率作为图谱特征参数,借助分类器算法对慢性胃炎气滞证与非气滞证的口腔呼气气味图谱特征进行模式识别,比较慢性胃炎气滞证患者常见病位的口腔呼气气味图谱特征。结果:慢性胃炎气滞证的主要病位证素分布是胃(91.18%)、脾(38.29%)、肝(23.68%);主要病位为胃、脾胃、肝脾胃、肝胃;采用分类器算法对慢性胃炎气滞证的气味图谱进行模式识别时,运用随机森林算法,对气滞证的准确率可以达到65.85%;病位胃组、脾胃组的气味图谱响应曲线B、C、D、E、F、I、J的振幅均显著低于病位肝脾胃组(P<0.01);病位胃组、脾胃组的气味图谱响应曲线C、D、E、F、I的斜率均显著低于病位肝脾胃组(P<0.01),脾胃组曲线A的斜率低于病位肝脾胃组(P<0.05)。结论:运用电子鼻结合模式识别方法可初步判断慢性胃炎气滞证及其不同病位间的口腔呼气气味差异。国家自然科学基金项目(No.81373552);;福建省自然科学基金项目(No.2014J01362);;福建省中医药科研项目(No.wzpw201313);;福建省教育厅A类项目(No.JA14212);;载人航天领域预先研究项目(No.020104)~

Cloning and Expression of the Leukotoxin Gene SH from Fusobacterium necrophorum

坏死梭杆菌白细胞毒素是坏死杆菌病的主要致病因子,白细胞毒素基因(lkT)是其编码基因。以分离到的国内牛源坏死梭杆菌fn(A)菌株f4基因组dnA为模板,应用PCr方法扩增白细胞毒素基因SH片段,克隆至PMd18-T载体上,以bAMHⅠ和HIndⅢ酶切的目的片段SH与相应酶切的PET32A载体连接构建PET32A-SH重组表达质粒,经转化E COlI bl21(dE3)后用IPTg进行蛋白诱导,SdS-PAgE检测重组蛋白表达情况。结果表明:扩增基因序列大小为1800bP,SdS-PAgE检测重组蛋白有效表达,表达得到大小为80.2kdA的目的蛋白,采用镍柱亲和层析方法纯化SH重组蛋白,获得了纯度达95%的重组蛋白;经WEST-Ern-blOT证实,该蛋白对抗坏死杆菌阳性血清具有反应活性。The leukotoxin of Fusobacterium necrophorum(FN) is considered to be one of the main virulence factors.The lkt gene encodes for FN.In this study,the SH fragment of lkt gene was amplified by PCR using the F4 genome as the template,which was isolated from the Chinese Fusobacterium necrophorum strain.The fragment was then cloned to the pMD18-T vector for sequencing.Thereafter,the SH fragment was subcloned into the multiple cloning sites of the pET32 to construct pET32a-SH recombinant plasmid,which was then trans-formed into E.coli BL21(DE3) with IPTG induction for expression.SDS-PAGE was used to analyze the recombinant protein.The results showed that the SH fragment of about 1800 bp was amplified and was about 80.2 kDa.The fusion protein was purified by Ni-NTA affinity chromatography under denature conditions,and their purity was above 95%.Western-blot analysis indicated the SH fragment had anti-genicity against Fusobacterium necrophorum.“十五”国家科技攻关子课题(2002BA518A04);吉林省科技发展计划项目(20070570);吉林市科技发展计划项目(200805

Cloning and Expression of the Leukotoxin BSBSE Gene from Fusobacterium necrophorum

以牛源坏死梭杆菌FNn株为试材,根据GenBank上已发表的坏死梭杆菌AF312861标准菌株的lktA序列设计1对引物,利用PCR技术扩增出1 131 bp的坏死梭杆菌白细胞毒素BSBSE基因。将PCR产物插入pGEM-T Easy vector中,经双酶切鉴定正确后进行序列测定。分析表明该BSBSE序列与GenBank上已发表的坏死梭杆菌AF312861标准菌株的lktA序列的核苷酸同源性为99%,推导出的氨基酸序列同源性为98%。为研究BSBSE的免疫原性,构建了原核表达载体pMAL-p2X-BSBSE,用IPTG诱导在大肠杆菌中表达。结果表明,BSBSE基因在大肠杆菌中进行了高效特异性融合表达,融合蛋白分子量约为84.5×103,其中41.5×103为BS-BSE基因表达的蛋白质,43.0×103为MBP融合标签,Western-blotting检测表明该表达产物有免疫原性。According to the sequence of announced lktA gene in Fusobacterium necrophorum,a pair of primers were designed.The BSBSE gene was amplified by PCR.The product was cloned into pGEM-T Easy vector.When nucleotide sequence and deduced amino acid sequence were compared with homologous sequence of the FN AF312861 lktA of GenBank,the homologue of the mucleotide sequence is 99% and the homologue of the amino acid sequence is 98%.The BSBSE fragment was inserted into expression vector pMAL-p2X and the plasmid pMAL-p2X-BSBSE were expressed in E.coli BL21 by IPTG induction.The SDS-PAGE analysis indicated the weight of the fusion protein was about 84.5.0×103,which included the 41.5×103 protein expressed from BSBSE gene and 43.0×103 fusion MBP tag.The recombinant BSBSE-pMAL-p2X production has Immunogenicity with western-blotting.The cloning and expression of the BSBSE gene established the foundation of further research on the function and application of the BSBSE gene.“十五”国家科技攻关子课题(2002BA518A04);; 中国农业科学院特产研究所科研基金项目(Tcs2005-03

Twinning in Nanocrystalline Ni by Severe Plastic Deformation

Deformation twinning is confirmed upon large plastic deformation in nanocrystalline (nc) Ni by transmission electron microscopy examinations. New and compelling evidence has been obtained for several twinning mechanisms that operate in nc grains, with the grain boundary emission of partial dislocations determined as the most proficient. Twinning in nc Ni may be interpreted in terms of molecular dynamics simulation based on generalized planar fault energy curves

系统性红斑狼疮患者骨髓基质细胞因子表达及对免疫功能的影响

【目的】研究系统性红斑狼疮（SLE）患者骨髓基质细胞因子表达情况,及其对外周血淋巴细胞增殖反应的影响,探讨SLE的发病机制。【方法】采用ELISA方法观察SLE患者骨髓基质细胞培养液IL-6、MIP-1、IFN-γ、TGF-β等细胞因子的表达,采用四甲基偶氮唑盐比色法（MTT）,观察基质细胞培养上清液对外周血淋巴细胞增殖反应的影响。【结果】SLE患者骨髓基质细胞培养液IL-6、MIP-1、IFN-γ浓度明显增高,TGF-β则降低,3种细胞因子与SLEDAI评分相关;正常人及SLE患者骨髓基质细胞上清液均对刀豆蛋白A诱导的外周血淋巴细胞增殖有明显的抑制作用,而SLE患者的上清液抑制作用明显低于正常人;在分别加入抗MIP-1、抗IFN-γ单抗后,骨髓基质细胞上清液对外周血淋巴细胞增殖的抑制作用明显增强,而加入抗TGF-β单抗后,抑制作用明显减弱。【结论】SLE患者骨髓基质细胞对外周血淋巴细胞增殖的抑制作用较弱,与其部分细胞因子异常表达有关,骨髓基质细胞可能与SLE的发病、发展有一定关系