Effect of Yuhan Nuanwei Cream Acupoints Stiking Therapy on Gastric Mucosa TNF-α,PCNA of Mice with Chronic Atrophic Gastritis

目的:研究御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎(CAG)大鼠胃黏膜肿瘤坏死因子-α(TNF-α)、增殖细胞核抗原(PCNA)表达的影响,探讨御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变CAG大鼠胃黏膜损伤修复的作用机制。方法:大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、药物对照组,采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,采用酶联免疫分析法测定胃黏膜细胞中TNF-α、PCNA的表达水平。结果:与正常组比较,模型组的大鼠胃黏膜组织病理学检查提示存在腺体的萎缩和一定程度的细胞异型增生,大鼠胃黏膜细胞中TNF-α、PCNA的表达水平升高,差异有统计学意义(P<0.05);与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜组织的病理损伤得到修复,大鼠胃黏膜细胞中TNF-α、PCNA的表达水平降低,差异有统计学意义(P<0.05);结论:御寒暖胃膏贴敷胃经穴可以促进CAG大鼠胃黏膜损伤的修复,该作用可能是通过调节胃黏膜细胞TNF-α、PCNA的表达水平来实验的。Objective:To study the effect of Yuhan Nuanwei cream(YNC)acupoints stiking therapy on gastric mucosa tumor necrosis factor-α(TNF-α) and proliferating cell nuclear antigen(PCNA) expression of mice with chronic atrophic gastritis(CAG),so to discuss the renovation mechanism of YNC on CAG precancerous lesions. Methods:The mice were divided into normal group,model group,YNC group,drug control group. Duplicate mice model with CAG precancerous lesions by the method of comprehensive intervention,observe gastric mucosal lesion index of mice with the naked eyes,observe pathological change of gastric mucosa tissue under light microscope,measure expression level of TNF-α and PCNA in gastric mucosa cells by the method of enzyme-linked immunoassay. Results: Comparing with normal group, mice gastric mucosa histopathological examinations in model group reminded that there exist gland atrophy and some degree of cell atypical hyperplasia,the expression level of TNF-α and PCNA in mice gastric mucosa cell are increased,differences being significant(P<0.05). Comparing with model group,pathological damage of mice gastric mucosa tissue in YNC group and drug control group obtained obvious repair,the expression level of TNF-α and PCNA in mice gastric mucosa cell were dropped, differences being significant(P<0.05).Conclusion:The therapy of YNC acupoints stiking therapy can promote the renovation of CAG mice gastric mucosal lesion. This fuction may be experimented by regulating the expression level of TNF-α and PCNA in mice gastric mucosa cell.深圳市科技研发资金项目(JCYJ20130401105615482

艾灸预处理对胃黏膜细胞调控作用的研究进展

本文介绍了近年来艾灸疗法对胃黏膜损伤的预保护作用的实验研究进展,主要讨论了艾灸调控胃黏膜细胞增殖与凋亡相关信号通路对胃黏膜损伤的预保护作用,并在此基上提出目前艾灸在预防胃黏膜损伤相关疾病研究中存在的问题与展望

基于“热证可灸”观察艾灸胃经穴对胃热证大鼠胃黏膜损伤修复作用的影响

目的:观察艾灸对胃热证大鼠胃黏膜组织形态的影响。方法:70只Wistar大鼠按随机数字表法随机分为7组,分别为:正常组、胃热证组、胃热艾灸组、胃热艾灸对照组、胃寒证组、胃寒艾灸组、胃寒艾灸对照组。除正常组外,胃热组用辣椒素和乙醇溶液灌胃,胃寒组用冰水灌胃及凉水泡浴等分别复制胃热证、胃寒证模型。造模后除正常组、胃热证组、胃寒证外,其余分别予艾灸干预12天。结果:与正常组比较,胃热证组大鼠躁动、兴奋,胃寒证组精神不振、踡缩、懒动等,胃热证组和胃寒证组的胃黏膜损伤指数明显升高(P <0. 05),胃黏膜组织病理学观察可见黏膜坏死脱落,溃疡形成,间质出现水肿,细胞间隙增宽明显,炎细胞浸润广泛;与模型组比较,胃热艾灸组可以改善大鼠躁动、兴奋等胃热证的证候,胃寒艾灸组可以改善大鼠精神不振、踡缩、懒动等胃寒证的证候,胃热艾灸组和胃寒艾灸组的胃黏膜损伤指数明显下降(P <0. 05),胃热艾灸组和胃寒艾灸组的胃黏膜组织病理学观察可见胃黏膜浅层有细胞脱落,无明显溃疡形成;胃热艾灸对照组和胃寒艾灸对照组无论在一般行为学的证候方面,还是在胃黏膜损伤指数和组织病理学方面均未见明显变化;胃热证组和胃寒证组大鼠的胃黏膜细胞中NT和Survivin的表达下降,艾灸胃经穴可以上调胃黏膜细胞中NT和Survivin的水平,而艾灸对照组则未见明显变化。结论:艾灸可以促进胃热证大鼠胃黏膜损伤修复,在一定程度上证明\"热证可灸\"具有可行性。广东省自然科学基金,编号:2017A030313748;;\n广东省中医药局中医药科研项目,编号:20191294;;\n深圳市卫生计生委科研项目,编号:201607061;;\n深圳市福田区卫生公益性科研项目,编号:FTWS2016003

御寒暖胃膏穴位贴敷对胃癌前病变大鼠胃黏膜的影响

目的:研究御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变大鼠胃黏膜的影响,探讨御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤修复的作用机制。方法:大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、御寒暖胃膏贴敷对照点组、药物对照组,采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,彩色多普勒观察胃黏膜的血流量。结果:与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜损伤指数值均显著降低(P0.05),胃黏膜组织的病理损伤未得到明显修复,胃黏膜血流量未见显著升高(P>0.05)。结论:御寒暖胃膏贴敷胃经穴可以促进慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤的修复,增加胃黏膜的血流量,并且存在经脉-脏腑特异相关性。深圳市科技研发资金项目(JCYJ20130401105615482

集束孔掏槽爆破破岩机理研究

掏槽爆破是竖井掘进爆破的常用手段，集束孔爆破用于掏槽爆破可有效提高掏槽效率。集束孔掏槽爆破过程涉及到结构在动力载荷下的从连续介质到非连续介质过程，数值计算过程复杂，如何快速估计某特定集束孔设计的爆破效果一直是科研界与工程界研究重点之一。本文基于应力波叠加方法提出了爆破区半径快速计算公式，同时基于连续–非连续单元法对5孔集束孔爆破设计展开了理论与数值分析。通过与实验结果对比，验证了计算公式的有效性，并讨论了集束孔掏槽爆破的破岩机理与规律。结果表明，当集束孔孔间距为4～5倍炮孔直径时，爆破效果最佳。本文相关成果可为爆破设计人员提供一定指导

Computational model for polarization degree of airlight under condition of near-horizontal observation

在光电成像领域,偏振滤波抑制大气背景光是提高成像质量的重要技术途径,其前提条件是分析和计算近水平观测条件下大气背景光偏振度。经典的Rayleigh散射理论和Wilkie天空光偏振模型在近水平观测条件下计算大气背景光偏振度存在较大误差。通过改进Wilkie模型,给出以观测地的经纬度、观测日期和时间、观测方向及大气能见度为输入的近水平观测条件下大气背景光偏振度的计算模型。实验表明:该模型在近水平观测条件下取得比Rayleigh散射理论和Wilkie天空光偏振模型更精确的大气背景光偏振度计算结果。此外,分析了偏振片消光比对大气背景光偏振度测量误差的影响

浅埋煤层开采诱发地下水渗流过程

针对榆神矿区覆岩层的红土层遇水展现出的裂隙张开-弥合效应，采用连续-非连续单元法(continuous-discontinuous element method, CDEM)研究表征黏性土层塑性变形特征的力学本构，对该地区黏土层中裂隙张开-弥合过程重现，并对浅层煤层开采诱发的水-力渗流过程展开分析。结果表明，红土层遇水膨胀，对周围土体产生挤压作用，部分裂缝会发生弥合现象，破裂度和导水裂隙带减少，土体体应变增大，整体位移上升，模拟结果的导水裂隙带高度与经验公式对比，相对误差为4%。与页岩水力压裂试验结果对比，裂缝发育面积、位置和厚度基本一致，验证了该模拟的有效性

压敏点恢刺法治疗枕神经痛60例

枕神经痛,是枕大神经痛、枕小神经痛以及耳大神经痛的总称[1]。其临床表现为一侧或双侧后枕、枕顶部持续性钝痛,并且伴有阵发性的顶枕部、外耳、乳突部针刺样、抽掣样疼痛,疼痛部位与枕神经的走行一致,相应部位的皮肤可有痛觉过敏及感觉减退。在枕神经的浅出口处常常有明显的压痛敏感点或痛性结节,按压时常伴有向上放射痛。目前现代医学治疗枕神经痛轻者予以口服非甾体类止痛药为主,重者则行神经阻滞等介入性治疗,然而仍未

The Influence of Cold-Removing and Stomach-Warming Ointment on Gastric Precancerous Lesions in Rats

目的：观察御寒暖胃膏对胃癌前病变大鼠胃黏膜的影响。方法：将50只SD大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、御寒暖胃膏贴敷对照点组和药物对照组,每组各10只。采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,正常组喂饲标准饲料,不予任何处理。模型组自第21周开始,每天捆绑1 h,并予胶布贴敷腹部及双下肢,每只每天按0.001 m L·g~（-1）的剂量经口灌服纯净水。御寒暖胃膏贴敷胃经穴组、对照点组自第21周开始,每天将各组大鼠捆缚于鼠板,将膏药涂于所选部位上,每日1 h。每只每天按0.001 m L·g-1的剂量经口灌服纯净水。药物对照组给予维酶素溶液,按21.6 mg·kg~（-1）的药量给药,每日1次,每天捆绑1 h,并予胶布贴敷腹部皮肤及双下肢。肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,彩色多普勒观察胃黏膜的血流量。结果：与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜损伤指数值均显著降低,差异具有统计学意义（P〈0.005）;胃黏膜组织的病理损伤得到明显的修复、胃黏膜血流量均显著升高,差异具有统计学意义（P〈0.005）。御寒暖胃膏贴敷对照点组大鼠胃黏膜损伤指数值未见明显降低,差异无统计学意义（P〉0.005）;胃黏膜组织的病理损伤未得到明显修复、胃黏膜血流量未见显著升高,差异无统计学意义（P〉0.005）。结论：御寒暖胃膏贴敷胃经穴可以促进慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤的修复,增加胃黏膜的血流量,并且存在经脉—脏腑特异相关性。Objective： To observe the influence of Cold-Removing and Stomach-Warming Ointment on gastric precancerous lesions in rats. Methods： 50 SD rats were randomly divided into the normal group,the model group,Cold-Removing and Stomach-Warming Ointment stomach meridian points group,Cold-Removing and Stomach-Warming Ointment control point group,drug control group,with 10 rats in each group. Gastric precancerous lesions in rats models were copied by using comprehensive intervention method.The ones in the normal group were fed with standard diet without any treatment. Since the twenty-first week,the ones in the model group were tied up for one hour,with belly and lower limbs being applied,and took pure water orally with the amount of 0. 001 m L·g~（-1） each day. Since the twenty-first week,the ones in Cold-Removing and Stomach-Warming Ointment stomach meridian points group,Cold-Removing and Stomach-Warming Ointment control point group were bounded to the rats board and applied ointment on the selected points,one hour each day. Each rat in the two groups took pure water orally with the amount of 0. 001 m L·g~（-1） each day. The ones in drug control group were treated with Dimensional enzyme solution with the amount of 21. 6mg·kg~（-1）each day. They were tied up for one hour each day,their belly and lower limbs were applied ointment with adhesive tape. Then the gastric mucosal injury index was observed by naked eye,and the pathological changes of gastric mucosa were observed under light microscope,and the blood flow of gastric mucosa was observed by color Doppler. Results： Compared with the model group,indexes of gastric mucosal injury in Cold-Removing and Stomach-Warming Ointment stomach meridian points group,and drug control group were significantly reduced,the difference was statistically significant（ P〈0. 005）. Their pathological damage of gastric mucosa was significantly repaired and the gastric mucosal blood flow was significantly increased,and the differences were statistically significant（ P深圳市科技研发资金（编号：JCYJ20130401105615482