Effect and mechanism of TIPE3 interference plasmid on SW480 colorectal cancer growth

目的:通过TIPE3干扰质粒转染SW480结肠癌细胞,验证干扰TIPE3表达对SW480结肠癌细胞生长的影响并探讨相关机制。方法:构建TIPE3; -shRNA-pSIREN-RetroQ干扰质粒,通过脂质体转染法成功将干扰质粒导入SW480细胞,通过RT-PCR、Western; blot检测重组质粒的干扰效率。应用CCK-8方法检测SW480细胞生存率。AnnexinV-FITC/PI流式细胞法检测细胞凋亡。使用West; ern; blot检测细胞增殖、凋亡相关分子的表达情况。结果:成功设计、构建和筛选具有生物活性且干扰效率最佳的TIPE3-shRNA-pSIREN-Ret; roQ干扰质粒。CCK-8检测证实干扰SW480结肠癌细胞TIPE3表达可以抑制细胞生长。流式结果显示,TIPE3-shRNA3干扰组的凋亡率为; (27. 991. 087) %,显著高于正常细胞组(12. 102. 213) %及转染空载质粒组(11. 440. 277 0); %。证实了降低TIPE3表达可以增加SW480对aDR5ScFv所诱导的细胞凋亡敏感性。Western; blot结果显示干扰TIPE3表达可以活化caspase3蛋白,降低p-AKT、p-PDK1、PCNA等分子的表达。结论:干扰TIPE3的表达对; SW480结肠癌细胞具有促进凋亡,抑制增殖的作用。Objective: To study the effect of interference TIPE3 on the colon cancer; cell growth by transfecting SW480 colon cancer cells with the TIPE3; interference plasmid were detected. Methods: Transfecting the; constructed TIPE3-shRNA-pSIREN-RetroQ plasmid to SW480 cells. To; determine the highest interference efficiency plasmid, the mRNA and; protein levels of recombined plasmid were detected by RT-PCR and Western; blot separately and tested the cell proliferation with CCK8. Meanwhile,; apoptosis rate of SW480 cells was determined by flow cytometry assay; with AnnexinV-FITC/PI. To further determined the effects of recombined; plasmid on cell development, the level of protein involved in; proliferation and apoptosis were detected by Western blot. Results: The; most effecient interference plasmids were successfully constructed. We; found that the cell survival rate decreased when interference TIPE3 gene; expressing in colorectal cancer cells. Flow cytometry indicated that; interefering the expression of TIPE3 would increase the sensitivity of; SW480 cell to apoptosis induced by aDR5ScFv. The results of Western blot; showed that low expression of TIPE3 would activate caspase3 and; downregulate the expression of p-AKT,p-PDK1 and PCNA. Conclusion:; Interference TIPE3 could promote apoptosis and inhibit proliferation in; SW480 colon cancer cells.国家自然科学基金; 福建省属公益类科研院所基本科研专项项

基于LTE230长岛电力无线专网建设与研究

本文针对在海岛环境下建设LTE230系统,通过分析长岛的环境状况和对建设条件的描述,并经过严格的选址要求,着重介绍了LTE230系统架构的搭建,具有成本低、覆盖广等特点。在复杂的环境下,对比传统的通信网络,可以发挥其良好的优势,满足了当地智能电网的发展需求。烟台科技项目（编号：5206051400K9）资

Dieulafoy病的诊断与治疗(附9例病例分析)

目的　探讨Dieulafoy病的临床特点诊断及治疗方法。方法　回顾性分析9例Dieu lafoy病的临床表现、诊断、治疗方法。结果　均为上消化道大出血。胃镜确诊8例。手术确诊1例,胃镜下治愈7例。手术治愈2例。结论　提高对本病认识,急诊胃镜是首选诊断方法。内镜下止血为首选治疗方法,反复出血应及时转外科手术

Development and Application of Micro-Electro-Mechanical System

【中文文摘】微机电系统是在微电子技术基础上产生和发展起来的多学科交叉的前沿科学研究领域 ,是面向 2 1世纪的高新科技 .介绍了微机电系统产生的背景影响、组成特征和基础研究内容 ,综述了微机电系统技术基础所涉及的材料、微机械设计和模、微细加工技术以及微封装与测试等领域 ,并对微机电系统的应用、典型的微器件、国内外的发展现状及前景进行全面分析 .在此基础上 ,论述了MEMS技术目前存在的问题和未来发展的趋势 【英文文摘】Micro-Electro-Mechanical System(MEMS) developes on the basis of microelectronics.It is a scientific researches frontier of multi-disciplines and a high technology in the 21st century.This paper presents the background,characteristics and basic research.The principal techniques of MEMS such as material,design and simulation,micromachining,packaging and testing are reviewed,Typic microapparatus,application prospect and the state-of-art of MEMS are also discussed in this paper.Furthermore,some points of view of developing on MEMS in the future are put forward

The Application of PLC in Pneumatic Control Electrode Board Cutting Machine

【中文文摘】本文详细阐述了气动控制系统、光电检测、可编程控制器在气动电极板剪切机中的应用。 【英文文摘】The pneumatic control system,optoelectronic detection system,the application of PLC in pneumatic control electrode board cutting machine was introduced in detail

Construction and identification of interference plasmid targeting on TNFAIP8

目的:构建并筛选出干扰效率最佳的TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒。方法:通过生物软件选择3个TnfAIP8基因干扰位点,构建干扰质粒并测序验证,将干扰质粒及对照质粒分别转染至A549细胞,通过rT-PCr、WESTErn blOT检测干扰效率。结果:经rT-PCr和WESTErn-blOT证实TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒能有效干扰并抑制细胞内TnfAIP8基因的表达,通过流式检测发现降低TnVAIP8表达可以提高细胞对A dr5SC fV诱导凋亡的敏感性。结论:成功构建和设计了对TnfAIP8基因具有显著干扰效率的干扰质粒,为进一步研究TnfAIP8基因的功能奠定了基础。Objective: To construct and screen the high efficiency interference plasmid of TFAIP8-shRNA-p SIRENRetro Q.Methods: Selected and synthesized three Target Sequence of TNFAIP8 shRNA1,TNFAIP8 shRNA2,TNFAIP8 shRNA3,and construct the TNFAIP8 interference plasmid.Transfection TNFAIP8-shRNA-p SIREN-Retro Q interference plasmid to A549 cells.Filter out the highest interference efficiency plasmid by detecting the mRNA and protein levels using RT-PCR and Western blot methods.Results: We successfully design and built three TNFAIP8-shRNA-p SIREN-Retro Q interference plasmids,and screen out the highest efficiency interference plasmid.Conclusion: Three interference plasmids targeting the TNFAIP8 gene have been constructed successfully and provide a useful tool for studying the function of TNFAIP8.国家自然科学基金项目(81272720); 福建省卫计委医学创新课题(2014-CXB-43); 厦门市科技计划项目(3502Z2083008)资

The overview of rapid prototyping technique

【中文文摘】在研究快速原型技术发展历史和现状的基础上,介绍了快速原型制造的基本原理,重点介绍几种发展较成熟的RP技术的基本原理,分析RP技术的特点,并探讨其应用价值和发展前景,提出了目前发展快速成型的基本观点和未来发展趋势。 【英文文摘】Based on the history and development of rapid prototyping technique,the principles of Rapid Protyping(RP),especially several developed techniques,are introduced.The characteristics of RP techniques are analyzed and the opinions about future development tendency and prospect are put forward.福建省自然基金项目(k32014);; 国家留学回国人员基金资助项目(k3003

计算机辅助测量联合3D打印技术在Taylor空间支架治疗小腿畸形中的应用

目的探讨运用计算机辅助测量联合3D打印技术在Taylor空间支架治疗小腿畸形中的可行性及临床效果。方法回顾性分析2016年5月至2018年10月在福建中医药大学附属厦门第三医院运用Taylor空间支架治疗小腿畸形的6例病人的临床资料。通过CT薄层扫描,3D打印技术制作骨模型和截骨导板。根据Taylor空间支架配套软件所需参数应用Mimics软件进行测量,按照配套软件的规划在3D打印模型验证矫形结果。术后参照Johner-Wruhs评定标准进行患肢功能评价。结果 6例病人均获得随访,随访时间为9～25个月,平均15.2个月。其中1例钉道感染,未发生神经、血管损伤和骨不连。拆除外固定时间为12～16周,平均13.7周。术后患肢无跛行。末次随访按照Johner-Wruhs评定标准,优5例,良1例。结论计算机辅助测量联合3D打印技术在Taylor空间支架治疗小腿畸形中可获得良好的临床疗效,并具有测量准确,截骨精确,矫形精准,调整方便等优势,是小腿复杂畸形精准化矫形的有效治疗方法

ERK干扰质粒的构建及对胃癌细胞株BGC823 DcR3表达的影响

目的探讨胃癌发展的分子机制,通过构建ERK1/2shRNA真核表达载体,体外研究其对人胃癌细胞株BGC823ERK1/2蛋白及DcR3表达水平的影响。方法应用PRNAT-U6.1/Neo载体构建ERK1/2基因shRNA重组质粒,经脂质体法导入BGC823细胞中,分别设置对照组、干扰组和U0126抑制剂组。Westernblot法检测转染后BGC823细胞及抑制剂使用后ERK1/2蛋白的表达变化,荧光显微镜检测质粒自带GFP基因的表达情况确认转染效率,ELISA法检测各组细胞上清中DcR3分泌蛋白的表达特点。结果成功构建ERK1/2基因shRNA重组质粒。证明了ERK1/2蛋白的表达与DcR3的分泌水平在BGC823细胞株中呈正相关。结论 ERK1/2干扰质粒明显降低BGC823细胞的ERK1/2蛋白表达水平,ERK信号通路对DcR3的分泌具有重要调控作用,为其下游调控机制的研究奠定了基础

The expression of TNFAIP8 in gastric cancer and its clinical significance

目的通过检测胃癌组织中肿瘤坏死因子α诱导蛋白8(TnfAIP8)的表达,探讨其与胃癌临床病理参数的关系及与胃癌发生发展的关系,揭示胃癌的发病机制。方法采用免疫组化SP法,检测50例胃癌以及50例正常胃粘膜中TnfAIP8的表达。结果 50例正常胃粘膜组织中TnfAIP8无表达,而在50例胃癌组织中TnfAIP8阳性表达13例,TnfAIP8阳性率为26%。低分化胃癌组TnfAIP8阳性表达率明显高于中分化腺癌组(P<0.05);Ⅲ期胃癌组TnfAIP8阳性表达率明显高于Ⅱ期胃癌组(P<0.05);有淋巴结转移组TnfAIP8阳性表达明显高于无淋巴结转移组;TnfAIP8在不同性别和年龄的胃癌组织中无差异。结论 TnfAIP8的表达与患者的性别和年龄无关,而与胃癌的组织学分级、胃癌的TnM分期和淋巴结转移有相关性。TnfAIP8在胃癌组织中表达率较正常胃粘膜升高,差异有统计学意义(P<0.05),提示TnfAIP8可能参与了胃癌的发生、发展。Tumor necrosis factor-α induced protein-8(TNFAIP8) is a recently discovered antiapoptotic molecule.Recent data have demonstrated that TNFAIP8 is involved in the regulation of apoptosis,cellular signaling cascade,tumor proliferation and invasion,as well as metastasis.In this study,we aimed to investigate the expression of TNFAIP8 in patients with gastric cancer and their relationships with clinicopathology.We detected the expression of TNFAIP8 in 50 cases of gastric cancer and 50 cases of normal gastric tissues with SP immunohistochemical.In gastric cancer,the expression of TNFAIP8 was located in cytoplasm and/or nucleolus of tumor cells;the expression of TNFAIP8 in patients with gastric cancer(26%) was higher than that in the normal gastric tissues(0%,P﹤0.05).And the expression of TNFAIP8 in poor differentiated adenocarcinoma of gastric was higher compared with that in well differentiated(P < 0.05),the same difference was fund between Ⅲ andⅡ stages of adenocarcinoma.However,the expression of TNFAIP8 was on significant difference among different person with different age and gender.The results suggest that the expression of TNFAIP8 is not related with patient gender or age,but related with the stage of disease.And the expression of TNFAIP8 may be involved in the generation and development of gastric cancer.福建省自然基金(2010D009