A study on antioxidation of synechococcus sp. PCC 7942 withTrans-thymosin α1-gene in Mice

本试验室已在蓝藻聚球藻中高效表达了人源胸腺素α1(thymosinα1,Tα1)基因,为研究转Tα1基因聚球藻口服后的生物活性,本研究给小鼠灌服转Tα1基因聚球藻14d,研究其对小鼠谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(Cat)和超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量的影响,结果表明:转胸腺素α1基因聚球藻可显著提高小鼠心、肝与肾中GSH-Px活力(P<0.01);明显提高心脏Cat活性(P<0.01);显著降低肝脏中MDA的含量(P<0.01);但对SOD活力无明显作用。提示转胸腺素α1基因聚球藻较强的抗氧化作用。Human thymosin α1 gene was expressed effectively in Synechococcus sp. PCC 7942 and antioxidant effect of Synechococcus sp. PCC 7942 with trans-thymosin α1-gene in mice were investigated. Synechococcus sp. PCC 7942 with trans-thymosin al-gene were administrated orally 14d,Laters the results showed that the activities of glutathione peroxidase (GSH-Px) in heart、liver and kidney were increased significantly (P<0. 01);the activity of catalase (Cat) in heart was increased markedly;the content of malondialdehyde (MDA) in liver was decreased obviously (P<0.01). But no significant change in the activity of super-oxide dismutase (SOD) was observed. It indicated that Synechococcus sp. PCC 7942 with trans-thymosin α1-gene had obvious antioxidation in vivo.国家海洋863课题(项目编号:819-04-03);福建省自然科学基金项目(项目编号:c0010002

Preparation and determination of polyclonal antibody to thymosin α1

目的 :制备用于检测转胸腺素基因蓝藻表达产物的特异性抗体。方法 :用提纯的小肽 (2 8个氨基酸 )胸腺素α1与牛血清白蛋白偶联后作为抗原 ,采用皮下多点注射免疫的方法免疫大鼠。经过 3个月的免疫 ,获得抗Tα的多克隆抗体。结果 :用间接酶联免疫吸附 (ELISA)方法 ,测得抗体效价超过 40 96。蛋白印迹 (Westernblot)检测结果显示 ,该抗体能特异性地与胸腺素α1抗原产生明显免疫亲和反应。结论 :所制备的抗体具有很好的灵敏性和特异Objective:To prepare specific antibody against Thymosin α1 Methods:Thymosin α1(Tα1,28 peptide) was conjuncted with BSA as immune antigen,rats were immunized and the polycolonal antibody to Tα1 was obtained.Results:With ELISA detection,the titer of antibody was more than 1:4 096;Western blot analysis showed that the antibody can bind with Tα1 specifically.Conclusion:The prepared antibody against Tα1 has good specificity and reactivity and can be used to detect the expression products of transgenic cyanobacteria which expressed Tα1 gene.国家“863”课题资助!(No 819 0 4 0 3

Advances of research of Cyanobacterium genome

本文对近年来蓝藻基因组研究进展进行了综述.介绍了聚胞藻PCC6803基因组研究方法和成果,包括最佳分析软件的选择,所获得的基因组基本信息,以及后基因组研究的部分成果.蓝藻基因组间差异很大,文中介绍了其它蓝藻基因组的基本信息和在各个藻种里的重要发现.文章最后探讨了蓝藻基因组的研究意义和前景.The recent research advances of Cyanobacteria genome were reviewed in this paper. As the representative of genome of Cyanobacteria, the research　methods and results of genome of Synechocystis sp.strain PCC6803 wereintroduced, including the selection about most suitable analyzing soft, the genome message and some results of its post-genome research. For the much difference among cyanobacteria, the basic message and significant discovery of others Cyanobacteriagenome were described. Furthermore, the research meaning and future develoment of the Cyanobacteria genome were discussed

高压输电线路外力破坏分析及防范措施

近年来,外力破坏对输电线路造成诸多安全隐患。文章通过对高压输电线路外力破坏类型和防外力破坏工作难点进行分析,针对不同外力破坏类型,提出了具体的防范措施,使公司电网的安全性和稳定性得到了有效的提升。</jats:p

准循环LDPC码在大气激光通信中的应用研究

针对部分QC-LDPC（准循环低密度奇偶校验）码存在秩亏的问题,研究了一种满秩QC-LDPC码的构造方法,这种方法在降低编码复杂度的同时,还减少了短环数量。针对BP（置信传播）译码算法复杂度高的问题,提出了一种改进的BP译码算法,选择变量节点对数似然比值判决门限T=5.3和校验节点选择的边数q=3,在不同强度弱湍流条件下还能在一定程度上改善译码性能。研究表明,文章提出的LDPC码构造方法和译码算法在大气激光通信中具有一定的应用前景