反相高效液相色谱保留机理研究

一前言高效液相色谱(HPlC)是现代发展速度最快的分离分析方法之一。反相高液相色谱(rP-HPlC)是各种HPlC形式和方法中应用最广的形式和方法。因此,对其保留机理的研究有更重要的意义

紧密连接蛋白claudins应用于肿瘤治疗的进展

Claudins蛋白家族是组成紧密连接(Tight junctions,TJs)必不可少的骨架蛋白,在维持上皮和内皮细胞中的细胞极性、细胞间的粘附固定、细胞旁路的离子运输等发挥重要作用。近年来大量的研究结果证明,claudins在许多人类恶性肿瘤中异常表达。因此,claudins也被作为癌症治疗的潜在靶标。文中就claudin蛋白家族在肿瘤中的表达情况及其相关药物的研究进展进行阐述。国家自然科学基金(No.31870925);;\n传染病重大专项(No.2017ZX10202203-001)资助~

反相高效液相色谱分离分析蛋白质研究

反相高效液相色谱已广泛应用于有机物分析,在无机物分析方面也有不少应用。在反相色谱中C_(18)柱应用最多。如能研究扩大C_(18)的应用范围,则一台HPlC仪配用一支C_(18)柱,便可分析各式各样样品中的各种成分,最大限度发挥仪器的使用效率。国家自然科学基金课

Assessing ecological risks of heavy metals to marine organisms in the Jiulongjiang Estuary by species sensitivity distribution

采用物种敏感性分布法(SSd)构建常见重金属元素对海洋生物的SSd曲线,结合九龙江口水体5、8、11月份21个站位重金属调查数据,计算了九龙江口7种重金属(AS,Cd,Cr,Cu,Hg,Pb,zn)不同暴露浓度对海洋生物的潜在影响比例(PAf),并分析了在相应站位重金属复合污染生态风险(MSPAf)。结果表明,7种重金属中AS的生态风险最大(即PAf值最高);时间尺度上,5月份总的MSPAf较其他月份稍高;空间尺度上,西溪至海门岛(1~7号站位)污染较严重,其中又以位于西溪和玉枕洲的2号和5号站位的MSPAf为最。In the present work,a species sensitivity distribution( SSD) method was used to assess the ecological risk of common heavy metals to marine organisms.The ecological risk was characterized by potentially affected fraction( PAF) of species in relation to concentration of the toxic materials.According to the investigation data obtained at 21 stations in the Jiulongjiang Estuary in May,August and November,the PAFs of seven heavy metals( As,Cd,Cr,Cu,Hg, Pb,and Zn) to marine organisms were calculated.The results showed that As had the highest PAF among the seven heavy metals at each station of the Jiulongjiang Estuary.Spatially,the multi-substance PAF( msPAF) in May was higher than in other months.Temporally,the msPAF of the Stations 1-7( from the Xixi Stream to the Haimen Isle of the Jiulongjiang Estuary) suffered from heavier pollution.In particular,the pollution status of the Station 2 and Station 5 was the most serious among all the investigated stations.海洋公益性行业科研经费专项(201105015); 国家海洋局青年海洋科学基金项目(2011143); 国家自然科学基金项目(31101902); 福建省自然科学基金项目(2012J05074); 国家海洋局第三海洋研究所基本科研业务费专项(海三科2011006)资

1978～2008年中国湿地类型变化

分别基于美国陆地卫星(Landsat MSS/TM/ETM+)和中巴资源卫星(CBERS-02B)影像数据,以人工目视解译为主,完成了中国1978～2008年4期(基准年分别为1978,1990,2000和2008年)湿地遥感制图,并进行了大量的室内外验证.在此基础上,对我国湿地现状及近30年来湿地变化进行了初步分析,得到以下主要结论:(ⅰ)截止2008年,中国湿地面积约为324097km2,其中以内陆沼泽(35%)和湖泊湿地(26%)为主.(ⅱ)1978～2008年,中国湿地面积减少了约33%,而人工湿地增加了约122%.过去30年里湿地减少的速度大幅降低,由最初5523km2/a(1978～1990年)降为831km2/a(2000～2008年).(ⅲ)减少的自然湿地(包括滨海湿地和内陆湿地),其类型变化由湿地向非湿地转化的比例逐渐降低.初期(1978～1990年)几乎全部(98%)转换为非湿地;在1990～2000年间减少的自然湿地约有86%转化为非湿地,而在2000～2008年,这一比例下降为77%.(ⅳ)气候变化和农业活动是中国湿地变化的主要驱动因素,湿地变化在中国分为三大不同特征区域,即西部三省/自治区(西藏、新疆和青海)、北部两省/自治区(黑龙江和内蒙古)和其他省市区.其中西部区域尤其是青藏高原,湿地变化的驱动因子以气候增温为主;新疆湿地由于气候增温和农业活动共同作用造成变化不大.北部省/自治区的湿地变化则主要由农业活动引起;而其他省市区的湿地变化几乎完全受控于人类的农业经济活动

First Sagittarius A* Event Horizon Telescope Results. VIII. Physical Interpretation of the Polarized Ring

In a companion paper, we present the first spatially resolved polarized image of Sagittarius A* on event horizon scales, captured using the Event Horizon Telescope, a global very long baseline interferometric array operating at a wavelength of 1.3 mm. Here we interpret this image using both simple analytic models and numerical general relativistic magnetohydrodynamic (GRMHD) simulations. The large spatially resolved linear polarization fraction (24%–28%, peaking at ∼40%) is the most stringent constraint on parameter space, disfavoring models that are too Faraday depolarized. Similar to our studies of M87*, polarimetric constraints reinforce a preference for GRMHD models with dynamically important magnetic fields. Although the spiral morphology of the polarization pattern is known to constrain the spin and inclination angle, the time-variable rotation measure (RM) of Sgr A* (equivalent to ≈46° ± 12° rotation at 228 GHz) limits its present utility as a constraint. If we attribute the RM to internal Faraday rotation, then the motion of accreting material is inferred to be counterclockwise, contrary to inferences based on historical polarized flares, and no model satisfies all polarimetric and total intensity constraints. On the other hand, if we attribute the mean RM to an external Faraday screen, then the motion of accreting material is inferred to be clockwise, and one model passes all applied total intensity and polarimetric constraints: a model with strong magnetic fields, a spin parameter of 0.94, and an inclination of 150°. We discuss how future 345 GHz and dynamical imaging will mitigate our present uncertainties and provide additional constraints on the black hole and its accretion flow

Inorganic Analysis by Reverse Phase HPLC

本文对近年来反相高效液相色谱法分离分析无机离子的现状和发展进行了综述,并展望了该研究领城的发展前景,引用文献146篇。A review with 146 reFerences is given on the development and current status of inorganic ions analysis by reverse phase HPLC Some prospectives in the research Field are prospected

A study on the Determination of the Monomer of the Polymethylmethacrylate by Reverse Phase High PerFormance Liquid Chromatography

设计一种新的简易的分解样品及前处理方法,用寿命长、重现性好、能分析最多种化合物的反相C_(18)柱,选用便宜易得的甲醇水为流动相,分离各组分,在254nM检测,建立一个测定有机玻璃中甲基丙烯酸甲酯单体的新方法。方法的标准偏差为0.07%,变动系数为3.3%。回收率为101%,在工业上使用取得良好的结果。A method For the determination of methylmethacrylate in the Polymethylmethacrylate by RP-HPLC was proposed.The method For the decomposition of the sample and the pretratment For the sample is rapid and simple.The column used is C_(18) and the mobile phase is methanolwater.The detection wave is 254nm.The method proposed was satisFactory For the determination of practical samples with the standard diavition of 0.07%, coeFFicient of variation of 3.3% and the recovery of 101%

基于自发Raman散射分布式光纤测温系统设计

光纤传感器是一种新发展起来的传感技术。文章介绍了基于自发Raman散射的分布式光纤测温系统的应用及其结构设计,并着重分析了在脉冲光作用下系统的测温原理,同时设计了一个新型的基于薄膜滤光片的用于Raman散射的波分复用（WDM）组件

Study on the Determination of Inorganic Anions in Water by Reversed Phase HPLC

本文试验反相高效液相色谱法分离分析水中氯离子、硝酸根和硫酸根的条件,研究可能干扰、提高选择性和提高灵敏度的方法,建立应用于自来水和海水等实际样品的分离分析方法。A study on the determination of Cl---, NO_3--(-) and SO_4--(2-) in tap water and sea water by reversed phase ion pair HPLC was proposed.The separation of inorganic anions was accomplished on a RP-C_(18) column using ion pair chromatographic technique and the UV detector was used to detected anions at 320 nm by indirect photometric detection method.The mobile phase (pH5.0) composed of 2.0mmol/L tetrabutylammonium hydroxide (ion pair reagent) and 2.0 mmol/L Salicylic acid (visualization agent)in methanol-water (2: 98).Good separation and determination results were obtained.The detection limit (ng) of anions was as Follows: Cl---, 3.4; NO_3---, 25.8; SO4_--(2-), 9.6