《伤寒论》太阳病辨误救误探析

结合《伤寒论》中有关误诊误治的具体条文,从辨证失误、治法失误、体质因素等方面分析了太阳病篇中误治变证的产生原因、变局转归,总结出《伤寒论》中以脉测证、以症求证、以治定证、溯史断证、无中辨证等救误辨证方法。原文条文以明.赵开美复刻的宋本《伤寒论》为蓝本

基于SCI 发文的中国生态学研究态势文献计量分析

采用文献计量学的方法,基于《科学引文索引》数据库近十年的检索结果,定量分析中国生态学领域发文的主题、时间、作者、机构、合作国家、被引频次、期刊分布等数据,系统分析中国生态学领域近十年的研究发展态势、研究热点、研究投入主体、国际合作和研究水平等趋势和特征。基于检索得到的3316篇SCI论文数据,研究认为:我国生态学领域的研究发展迅速。研究的热点主要是分子标记、气候变化、生物多样性、土地利用、分类学、物种、草原、荒漠化、模型、多态性等方面。研究主题的关联性随时间有增加的趋势。588个第一作者发文机构中,中国科学院发文量最高,大学近年的研究发展迅猛。生态学领域篇均作者人数是2.2人。国际合作的主要国家是美国。我国生态学研究的SCI发文篇均被引频次为6。第一作者机构为中国机构的论文被引用情况逊于外国机构。中国生态学领域研究最重要的发文期刊是ECOLOGICAL MODELLING和JOURNAL OF ARID ENVIRONMENTS

外加剂对混凝土抗裂性能影响的试验研究

通过圆环试验,研究了4种常用减水剂和UEA膨胀剂对混凝土收缩开裂的影响。结果发现:聚羧酸系和萘系高效减水剂对净浆开裂龄期均有延缓作用,但影响规律却不尽相同。随着聚羧酸系减水剂掺量的增多,试件开裂龄期逐渐缩短,抗裂性能下降;而随着萘系减水剂掺量的增多,试件开裂龄期逐渐增长,抗裂性能提高。UEA膨胀剂在6%～12%范围的等量内掺,有明显的减缩作用

Experience in constructing the marine environment science national experimental teaching demonstration centre

结合建设国家级实验教学示范中心的经验,介绍了海洋环境科学实验教学中心改革与建设的思路、措施、成效和示范辐射作用,旨在加快实验教学改革和实验室建设,促进优质资源整合和共享,培养有创新精神和实践能力的高素质海洋环境科学人才。Based on the experience in constructing the national experimental teaching demonstration centre of marine environment,Xiamen University,elaborated on the guidelines,measures,objectives,the demonstration and radiating role,aiming to in speed up the step of experimental teaching reform and construction of laboratory,promote the superior resources integration and sharing,and cultivate the innovation spirit and the practice ability of the high-quality marine environmental science personnel

Identification of Colorants in Food by Surface-enhanced Raman Spectroscopy and Wavelet-based Reverse Search

使用金纳米粒子为增强因子的表面增强拉曼光谱技术,通过连续小波变换将拉曼光谱信号转化到小波空间(墨西哥帽小波作为小波基)。该步骤能够减轻信号中基线; 变化及随机噪音的影响并找到峰位置和最佳小波尺度系数。依据小波空间中的信息,对混合物光谱及标准谱光谱进行反向搜索得到反向搜索匹配系数(Revere; match quality,; RMQ),作为判断混合物中目标成分是否存在的依据。该算法可对混合物中的目标物质进行准确定性,并已成功应用于多种食品中色素鉴定。食品中色素的检出率; 达到99%,且结果稳健,其效果明显优于传统的命中质量系数法(Hit quality index,; HQI)。这证实了小波空间反向搜索方法是一种快速而准确的拉曼光谱定性算法。In this study, a gold nanocrystal colloid was used as the enhancement; factor for surfaceenhanced Raman scattering (SERS). Raman spectra were; transformed by continuous wavelet transform (CWT),and Mexican hat; wavelet were chosen as the wavelet basis. This procedure could be used; to alleviate the influence of baseline variations and random noise,and; find peak positions and the best scale wavelet coefficients of signal.; Reverse search method was proposed to compare the spectrum of an unknown; sample with a spectrum of standard using the information in wavelet; space. Reverse match quality (RMQ) could be obtained automatically to; determine whether a substance is present. It was used to identify; colorants in a variety of food successfully. The colorants could be; identified with 99 percent accuracy. It shows a better performance; compared with traditional hit quality index (HQI). The study confirmed; that the wavelet-based reverse search is feasible and accurate in; qualitative analysis.国家自然科学基金资助项目; 国家重大科学仪器设备开发专

MiR-127-3p靶向MAPK4对葡萄膜黑色素瘤细胞增殖、凋亡、迁移和侵袭的影响

【目的】探讨miR-127-3p对葡萄膜黑色素瘤细胞增殖、凋亡、迁移和侵袭的作用。【方法】通过RT-qPCR检测人葡萄膜黑色素瘤组织及细胞、正常组织及细胞中miR-127-3p与MAPK4mRNA的表达；通过Lipofectamine2000说明书将mimic-NC、miR-127-3pmimic、pc-MAPK4质粒分别或联合转染进入SP6.5或OM431细胞；通过双荧光素酶报告检测miR-127-3p与MAPK4复染靶向关系；通过CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡，划痕实验检测细胞迁移能力，Transwell法检测细胞侵袭能力，蛋白印迹法检测AKT/mTOR通路蛋白相对表达水平。【结果】在葡萄膜黑色素瘤组织和细胞系中，miR-127-3p表达明显下调（P<0.01），而MAPK4表达明显上调（P<0.01）；miR-127-3p与MAPK43′UTR区存在结合位点，miR-127-3p高表达明显抑制了含有野生型MAPK4质粒的荧光素酶活性（P<0.01），但对突变型MAPK4质粒的荧光素酶活性无影响；与Control组相比，miR-127-3pmimic组SP6.5细胞和OM431细胞增殖均明显下降（P<0.01），凋亡率均明显增加（P<0.01），划痕闭合率均明显降低（P<0.01），每视野侵袭细胞数目均明显减少（P<0.01），p-AKT（T308）/AKT、p-mTORr（S473）/mTOR蛋白表达均明显下调（P<0.01），共转染pc-MAPK4逆转上述变化。【结论】MiR-127-3p通过靶向下调MAPK4来抑制葡萄膜黑色素瘤细胞增殖、迁移和侵袭，诱导细胞凋亡，这可能与抑制AKT/mTOR通路激活有关

Distribution and polluting effects on Capitella capitata, Nephtys oligobranchia, Tharyx sp. on the intertidal mudflats in Shenzhen Estuary

在深圳河治理工程环境影响评估研究中 ,将 1 995年 7月至 1 998年 4月在 3个断面获得的 3种多毛类的数量进行分析 ,得出深圳河口泥滩 A断面和 RB断面的小头虫有明显的季节变化 ,即冬季数量较其他季节高 ,秋季数量较其他季节低 ,ET断面的小头虫数量没有明显的季节变化。RB断面寡鳃齿吻沙蚕数量随时间推移有增加的趋势 ,与有机质含量的变化是相似的。寡鳃齿吻沙蚕和独毛虫的水平分布明显受盐度限制 ,盐度较低的 ET断面寡鳃齿吻沙蚕数量较盐度较高的 A断面和 RB断面的低 ,独毛虫没有分布到 ET断面。 A断面的独毛虫在养猪场废水注入时大量繁殖。文中探讨了溶解氧和有机质含量与 3种小个体多毛类数量的关系。There are two famous Natural Reserve, Mai Po Marshes Reserve and Futian Natural Reserve, in Shenzhen (Deep) Bay. Deep Bay is an internationally important site for waterfowl and mangrove. Environmental stress in Mai Po and Fu Tian mudflats, induced by the accumulation of anthropogenic contaminants, such as heavy metals, poly-chlorinated biphenyl (PCB) and PAHs from industrial effluents, has aroused great concern after the Hong Kong Government declared Mai Po a wetland of international importance under the Ramsar Convention in September 1995.;High clay content, rich organic matter and high density of macrofauna are three ecological characteristics on intertidal mudflats in Shenzhen estuary. Capitella capitata, Nephtys oligobranchia and Tharyx sp. are commom species of small individual polychaete of the intertidal mudflats in Shenzhen Estuary. Capitella capitata is a worldwide species and it is considered as indicator of pollution. ;The data of three species of small individual polychaete got from July 1995 to April 1998 seasonly. Three transects including nine stations were designed for macrobenthic investigation. The study area was located in 114°00′18″E ～114°01′48″E, 22°29′51″N～22°31′66″N. Transect A includes stations A1, A2, A3 which are on the Shenzhen side of Shenzhen Bay. A3 is located near mangrove and the distances between A1 and A2, or between A2 and A3, were about 150 m. Transect RB and ET are on the Hong Kong side of the study area. Transect RB includes stations RBO, RB3 and 45O; Transect ET includes stations ET1, ET2 and ET4. Seasonal sampling was conducted in January (winter), April (spring), July (summer) and October (autumn). To collect benthic samples, a plastic core of 10 cm diameter and 20 cm length was inserted into the sediment to a depth 20cm. The sediment collected was transferred to a plastic bag and labeled with sample number. A total of five replicates were collected at each station. To wash the samples, they were transferred to a bucket and water was added. The slurry was then swung manually and then poured gently through a 0 5 mm sieve. The benthos and sand retained on the sieve were then transferred into bottle with formalin and Rose Bengal by forceps. The specimens were taken to laboratory for identification and counting under a microscope. After counting, three species of polychaete were dried in an oven at 60℃ for 48 hours. Regression analysis used the software SPSS 10.0. The seasonal variations of Capitella capitata, Nephtys oligobranchia and Tharyx sp. vary at three transects. Capitella capitata at transect A and transect RB had clearly seasonal variation. The quantities of Capitella capitata in winter were higher than that in other seasons in the same year. But there was no quantitative patterns for Capitella capitata at transect ET. There were increasing trends for the quantities of Nephtys oligobranchia at transect A and transect RB from July 1995 to April 1998, which was similar to the temporal patterns of organic matter. Regression analysis showed there was significant interrelation between the temporal patterns of organic matter and time. There was no any trend for the quantities of Nephtys oligobranchia at transect ET with the time going. The density and biomass of Tharyx sp. at transect A increased from July 1995 to April 1997, which may related to pig-cultural farms. There were many pig-culture farms near transect A before January 1997. The sewage of the pig-culture farms was discharged into the intertidal mudflat around transect A. There was no seasonal patterns for the density and biomass of Tharyx sp. at transect RB. Tharyx sp. was not found at transect ET during investigative period. ;The distributions of Capitella capitata, Nephtys oligobranchia and Tharyx sp. on the intertidal mudflats in Shenzhen Estuary were different in various transects. The average density and biomass of Capitella capitata (average value of three years) at transect A three years were lower than those at transect ET. But the average density and biomass of Nephtys oligobranchia at transec国家自然科学基金资助项目 (3 9670 1 3 5

Biogenic silica in surface sediments of the northeastern and southern South China Sea

本研究测定了南海东北部和南部海域表层沉积物的生物硅含量(SIO2%),其含量范围分别为1.08%—3.01%和0.79%—9.06%,平均值分别为1.76%和4.22%。研究结果表明,南海表层沉积物中的生物硅含量与站位水深呈正相关关系;南海东北部的表层沉积物中的生物硅含量与其中的矿物含量、铁离子浓度、间隙水中的营养盐浓度不存在明显的相关性;南海南部海域表层沉积物中的生物硅含量与烧失量、有机碳含量、碳酸盐含量呈正相关关系,与粘土矿物含量相关性不明显。Biogenic silica(BSi) contents in surface sediments from the northeastern South China Sea(NSCS) and southern South China Sea(SSCS) were measured.The BSi contents(SiO2%)were in the range of 1.08%–3.01% and 0.79%–9.06%,with the means of 1.76% and 4.22% for the NSCS and SSCS,respectively.The results showed that the BSi contents in surface sediments have positive correlation with water depth of the sampling locations.The BSi contents in the NSCS had no evident correlation with minerals,ferrum ion concentrations in surface sediments or nutrients in the pore waters of sediments.The BSi contents in the SSCS had positive correlations with organic material and carbonate contents but not with clay material contents.国家基础研究发展计划项目(2005CB422305

武汉东湖秋季水体中光合自氧生物膜的生长特性

利用一种自行设计的光合自氧生物膜采样装置,在武汉东湖(重富营养化湖泊)20和100 cm水深处自然培养和收集光合自氧生物膜,研究其生长发育及活性特点。结果表明:富营养化水体中光合自氧生物膜生长经历了潜伏适应期、增长期、脱落期和恢复生长期4个阶段;光合自氧生物膜生物量、胞外多糖含量、COD含量和脱氢酶活性随培养时间延长呈稳定增长趋势;20 cm水深处光合自氧生物膜生长总体上快于100 cm水深处,并且其生物量和活性也较高;光合自氧生物膜的生理特征与一般天然水体中生长的相似,说明重富营养化水体对生物膜的胁迫

Pathological characteristics of enterotoxigenic Escherichia coli K88 and screening of its probiotics

在获得绿色荧光蛋白(GFP)标记的菌株K88-GFP并证明其与K88具有遗传同质性的基础上,以K88-GFP为致病菌,腹腔注射侵染小鼠,在不同时间进行眼球采血,测定血液生理生化指标,并采取不同器官或组织,培养后利用紫外光激发K88-GFP的绿色荧光,观察计数这种产肠毒素性大肠杆菌(ETEC)在小鼠体内的分布。同时通过体外抑制和活体饲喂试验,进行了益生菌的筛选。结果证实,ETEC致病菌具有较强的侵袭性,它可以侵袭小鼠肝、肾、心、肺及脑、肌肉等器官和组织,尤其可对肝、肾造成严重的损伤;筛选得到益生菌株PB JK-2,在体内外均对K88具有较好的抑制作用。Healthy Kunming mice were infected with Escherichia coli strain K88-GFP,which was marked with green fluorescence protein(GFP) gene and shared homology with enterotoxigenic E.coli(ETEC) K88,by intraperitoneal injection.Eyeball blood of the infected mice was sampled and the standard blood biochemical indexes were determined at different moment post-injection.Different organs and tissues of the mice-killed at different time points post-injection were sampled and cultured.Then,the distribution of the ETEC in the mice was observed by ultraviolet radiating of the green florescence of K88-GFP clones.Probiotics were screened by using inhibition test in vitro and mice feeding test in vivo.The results showed that the ETEC was invasive,and could infect murine liver,kidney,heart,lung,brain and muscles but damage seriously the liver and kidney.Probiotics strain PB JK-2 was selected and proved to suppress the pathogeny K88 well in vivo and in vitro.福建省青年科技人才创新计划项目(2005J064