The Physiological Response of Synsepalum dulcifcum Denill Leaves to NaCl Stress

为明确神秘果(Synsepalum dulcifcum Denill)的耐盐能力,研究其在NaCl胁迫下的适应机制并丰富果树的耐盐机理,通过盆栽试验法,以0、2‰、4‰、6‰、8‰NaCl溶液分别对幼苗进行胁迫处理,测定叶片叶绿素、渗透调节物质、抗氧化物酶、丙二醛含量及土壤实际盐度。结果表明:在NaCl胁迫下,神秘果叶片的叶绿素合成受到明显抑制;超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性随浓度增加而显著升高,过氧化氢酶(CAT)活性则是先升后降,在4‰时达到最高;可溶性蛋白含量与处理浓度呈负相关;脯氨酸、可溶性糖含量和对照相比有显著增加,推测脯氨酸和可溶性糖在胁迫过程中起着重要的渗透调节作用。To clear the tolerance and adaptive mechanism of S. dulcifcum and to rich the theory about the salt tolerance mechanism of fruit trees. The potted plants were watered with 0, 2‰, 4‰, 6‰, 8‰ NaCl and the physiological characteristics including the changes about chlorophyll, soluble protein, soluble proline, soluble sugar,SOD, POD, CAT, MDA of NaCl stress in S. dulcifcum leaves were measured. The result showed that the chlorophyll synthesis was significantly restricted under NaCl stress. The activity of SOD or POD increased clearly with the NaCl concentration, and the CAT activity increased to a peak under 4‰ NaCl and then dropped. The content of soluble protein dropped under NaCl stress while that of proline, soluble sugar increased significantly. It was deduced that proline or soluble sugar working as osmotic potentials in cells played a key role in salt tolerance of S. dulcifcum.厦门市科技计划项目(No.3502Z20092023

当归芍药散加味方及拆方对老年鼠脑组织#br# 和淋巴细胞β-AR 密度的影响

【目的】研究当归芍药散加味方(DSS)及拆方对老年鼠脑组织和淋巴细胞β 肾上腺受体(β-AR)的含量的影响。 【方法】选用中老年鼠(18 个月), 分组灌服DSS 及拆方水煎液, 3 周后, 用放射配基受体结合分析法测定大鼠大脑皮质、海马、 小脑皮质、脾细胞和血液淋巴细胞β-AR 密度。【结果】与中老年对照组比较, DSS 全方组小脑皮质、脾细胞和血液淋巴细胞 β-AR 密度明显增多(P <0.01), 大脑皮质、海马β-AR 密度与老年对照组间差别不明显, 君臣组、佐药组各种组织β-AR 密度 与中老年对照组间差别均不明显(P >0.05)。【结论】上述结果表明DSS 全方可选择性提高老年鼠小脑皮质、淋巴细胞的β- AR 密度, 这是DSS 君、臣、佐药共同作用的结果

万山汞矿区弹琴蛙重金属富集与组织关联分析

为了解万山汞矿区弹琴蛙（Nidirana adenopleura）组织重金属富集特征及其关联，对万山汞矿区典型农田生境内弹琴蛙组织（肝脏、皮肤、股骨、肌肉和趾骨）中铬（Cr）、锰（Mn）、镍（Ni）、铜（Cu）、锌（Zn）、镉（Cd）、铅（Pb）、砷（As）、硒（Se）和汞（Hg）这10种重金属元素的生物富集特征差异进行分析，结合非负矩阵分解分析法识别重金属元素的来源及贡献，并通过灰色关联度分析法分析弹琴蛙组织重金属元素间的关联性。结果表明：重金属元素在万山汞矿区弹琴蛙体内富集程度不同，平均质量分数由大到小依次为Zn、Cu、Mn、Pb、Se、Cr、Hg、Cd、Ni、As；Cu、Se、Cr、Ni、Cd和Hg主要富集在肝脏组织中，Mn、Zn和Pb主要富集在股骨和趾骨组织中，As主要富集在肌肉组织中。基于非负矩阵分解和相关分析，Hg、Cu、Cr、Pb和Se主要源于汞矿开采， As主要来源于农业活动，Mn主要来源于锰矿冶炼，Ni、Zn和Cd来源于多种复合途径。总之，两栖动物趾骨具有较好的生物指示作用，可作为一种非致命性的检测方法，用于动物重金属污染的研究。研究结果可为万山汞矿区环境污染防控和野生动物保护提供科学依据

Soluble Sugars and MDA in Leaves of Two ManilkaraSpecies under Salt Stress

采用盆栽试验法,以0、2‰、4‰、6‰、8‰nACl溶液分别对铁线子属果树古巴牛乳树MAnIlkArA rOXburgHIAnA(WIgHT)dubArd、人心果MAnIlkArA zAPOTA(l.)VAn rOyEn进行胁迫处理,对植株盐害现象、土壤实际全盐度和叶片可溶性糖、丙二醛含量的变化进行比较。试验结果表明:古巴牛乳树、人心果分别在土壤实际全盐度3.38‰、2.88‰出现2级盐害,在4.28‰、4.05‰时出现3级盐害。2种果树叶片可溶性糖含量变化与nACl用量梯度呈现正相关,并和对照差异显著。叶片丙二醛含量在2‰、4‰nACl时和对照差异不显著,在6‰、8‰nACl时与对照差异显著。试验结果推断:2种铁线子属果树在6‰、8‰nACl处理时,叶片细胞膜脂过氧化加重;可溶性糖作为渗透调节物质对植物幼苗抵抗nACl胁迫可能起着重要作用。Mechanism of the salt tolerance of Manilkara roxburghiana(Wight)Dubard and Manilkara zapota(L.)van Royen was investigated.The potted plants of the two species were watered with 0,2‰,4‰,6‰ or 8‰ NaCl solutions to observe the damages to the seedlings,as well as the induced changes on soil salinity and on the soluble sugars and MDA in the plant leaves by the salt stress.M.roxburghiana and M.zapota showed a Grade II salt damage under 3.38‰ and 2.88‰ NaCl stress,respectively,and a Grade III damage under 4.28‰ and 4.05‰ salt stress,respectively.The soluble sugars in the leaves of the plants increased with increasing concentration of NaCl,and were significantly different from those of Control without the salt stress.It was speculated that,with respect to the cellular osmosis,the soluble sugars might have increased by the plant in response to the presence of salt so as to improve its tolerance to the stress.The contents of MDA also differed significantly from Control under 6‰ or 8‰NaCl stress indicating the presence of a serious membrane lipid peroxidation on the plants.厦门市科技计划项目(3502Z20092023

Modification of spin electronic properties of Fe-n/GaSe monolayer adsorption system

Ⅲ族金属单硫化物因其优越的光电和自旋电子特性而备受关注,实现对其自旋性质的有效调控是发展器件应用的关键.本文采用密度泛函理论系统地研究了GaSe; 表面Fe原子吸附体系的几何构型及自旋电子特性.Fe/GaSe体系中Fe吸附原子与最近邻Ga,Se原子存在较强的轨道耦合效应,使体系呈现100%自; 旋极化的半金属性.其自旋极化贡献主要来源于Fe-3d电子的转移及Fe-3d,Se-4p和Ga-4p轨道杂化效应.对于Fe双原子吸附体系,两Fe原; 子之间的自旋局域导致原本从Fe转移至GaSe的自旋极化电荷量减少,从而费米能级附近的单自旋通道转变为双自旋通道,费米能级处的自旋极化率转变为0.; 研究结果揭示了Fe_n/GaSe吸附体系自旋极化特性的形成和转变机制,可为未来二维自旋纳米器件的设计与构建提供参考.Group-IIIA metal-monochalcogenides have been extensively studied due to their unique optoelectronic and spin electronic properties. To realize the device applications, modifying their magnetic properties is desirable. Atomic doping and vacancy defects have been proven to produce itinerant ferromagnetism and half-metallicity in GaSe monolayer. Relatively, the magnetic modification by adsorbing foreign atoms is rarely reported. Traditional ferromagnetic material, Fe element, possessing high electronic polarizability and high Curie temperature, becomes the best option of adsorbate. In this work, Fe n (n = 1, 2) atoms adsorbed GaSe monolayer systems are constructed, and the spin electronic properties are systematically studied through the density function theory. Based on the geometric configuration of fully relaxed 3 x 3 GaSe supercell, three highly symmetrical sites, i.e., the hollow site, the top site of Se atom, and the top site of Ga atom are inspected to search for the stable absorption positions of Fe n atoms. Computation results of adsorption energies indicate that the top site of Ga atom is preferred for single Fe atom, and the hollow site near the first Fe adatom is the most stable site serving as adsorbing the second Fe atom. Based on the most stable configuration, the spin electronic properties are studied. For the single Fe adsorbed system, the valence band maximum moves to point, resulting in a direct-band-gap. The strong orbit coupling effect between Fe adatom and its nearest Ga and Se atoms causes un-coincident majority and minority spin channels. Two impurity bands are located near the Fermi level and contribute only to the minority spin channel, producing a half-metallicity with a 100% spin polarization in the system. Bader charge analysis and spin-resolved partial density of states suggest that the spin polarization is mainly attributed to the transfer of Fe-3d electrons, and the hybridizations of Fe-3d, Se-4p, and Ga-4p states. Charge transfer from the Fe adatom to GaSe generates an n-type doping and an antiferromagnetic coupling between Fe and vicinal Ga and Se atoms. For the two-Fe-atoms adsorbed GaSe monolayer, the spin electronic states are found to be mainly located between the two Fe adatoms, leading to the reduction of the charge transfer from Fe to GaSe ML. As the original single spin channel turns into two spin channels (majority spin channel and minority spin channel) near the Fermi level, the ferromagnetic coupling between Fe atom and the vicinal Se atoms turn into antiferromagnetic coupling and the spin polarization falls to 0%. Therefore, the spin properties of GaSe monolayer can be controlled by modifying the number of adsorbed Fe atoms. These results reveal the formation and transform of the spin electronic properties of typical ferromagnetic/GaSe adsorption system, which offers some advice for designing and constructing the two-dimensional spin nanostructures.National Natural Science Foundation of China [61674124, 11304257,; 11604275, 61227009]; National Basic Research Program of China; [2016YFB0400801]; Natural Science Foundation of Fujian Province, China; [2014J01026, 2016J01037, 2015J01028]; Fundamental Research Funds for the; Central Universities, China [20720160122, 20720150033, 20720160044