Study on the distribution and diversity of nanoflagellate in the Yangtze estuary and continental shelf of East China Sea

本研究应用荧光显微技术、荧光标记颗粒示踪法以及Illumina高通量测序技术，分别于2013年春季、2013年夏季和2013年秋季开展了对长江口及东海陆架区微型鞭毛虫的丰度分布、异养细菌的摄食和群落多样性的研究，并初步探讨了影响微型鞭毛虫分布、摄食和群落多样性的因素。主要结果如下： （1）长江口及东海陆架区微型鞭毛虫的丰度变化范围为0.41-7.15×103cellsml-1，微型鞭毛虫丰度的最高值出现在夏季，最低值出现在秋季，表现出明显的季节变化特征。基于丰度比得出的微型鞭毛虫营养结构也有着典型的季节特点：春季微型鞭毛虫以含色素体微型鞭毛虫（PNF）为主；夏季和秋季微型鞭毛虫以异养微型鞭毛...We carried out ecological studies on nanoflagellate(NF) in the Yangtze estuary and continental shelf of East China Sea. Through the use of the fluorescent microscope technique, fluorescent dye particle tracer method and illumina high-throughput sequencing, we investigated the spatial and temporal distributions, the grazing on heterotrophc bacteria and the community structure and diversity of nanof...学位：理学硕士院系专业：海洋与地球学院_海洋生物学学号：2242011115139

Construction of Raman spectroscopy system and implementation of algorithm based on shifted excitation method

拉曼光谱技术作为一种强有力的分子结构研究工具，广泛应用于材料物理、生物医学、食品安全等领域。然而很多物质比如药品、食品的拉曼光谱检测过程都伴有强荧光干扰，荧光的存在严重影响拉曼光谱特征峰的识别，此外探测器本身的噪声和暗电流严重影响着弱拉曼特征峰的探测，因此非常有必要采取措施抑制荧光干扰和提高拉曼光谱系统的信噪比。 移频激发法是一种简单通用的荧光抑制方法，它常采用两个波长相近的激发光源分别激发样品得到两幅拉曼光谱，对这两幅光谱作差分，从而有效地消除荧光的影响。本文研究了移频激发法的荧光抑制原理，并分析了从差分光谱中提取真正拉曼光谱的复原算法需要克服的干扰因素。此外，对多种复原算法进行了详细的推...Raman spectroscopy technique as a powerful tool to investigate the molecular structure is widely used in material physics, biomedical, food safety and other fields. However, Raman spectroscopy detection process of many substances such as pharmaceuticals and food is always accompanied by strong fluorescence interference. The existence of fluorescence seriously affects the recognition of the charact...学位：工学硕士院系专业：物理与机电工程学院_微电子学与固体电子学学号：1982012115278

渤海海峡跨海通道与胶东半岛、辽东半岛旅游一体化发展

胶东半岛、辽东半岛间“C”型交通现状阻碍其旅游一体化发展。渤海海峡跨海通道的建设将使得两大半岛地理上相互衔接,增加旅游客源,加快旅游资源要素的流动,帮助公众积极参与旅游战略合作规划,从而推动胶东半岛、辽东半岛旅游经济一体化发展,发挥其旅游资源一体化优势,促进两地旅游一体化可持续发展

Design and Simulation of Contact Fatigue Testing System of Flexspline and Circular Spline in Harmonic Drive

谐波齿轮传动是随着空间技术的发展而诞生的一种新型传动方式，其工作原理是利用柔轮的弹性变形波来实现运动或者动力传递，因其具有结构简单、传动精度高、传动比大等诸多优点，在航空、航天、机器人等领域得到了广泛应用。在谐波齿轮传动过程中，柔轮的运动情况比较复杂，作为弹性薄壁构件需承载交变应力，容易造成齿面磨损和疲劳断裂。因此，建立合适的模型对谐波齿轮传动过程中柔轮的错齿运动进行模拟分析具有重要意义。 本文首先针对谐波齿轮传动过程中柔轮的错齿运动设计了一种新的接触疲劳试验装置，根据机械设计原理对装置各个零件进行强度与刚度校核。并根据已知参数、实际工况与啮合参数，设计了柔轮与刚轮的基本结构。基于两者的齿形...Harmonic drive is a new driving method emerged with the development of space technology that utilizes the elastic deformation wave of a flexspline to achieve motion transmission or power transmission. It has many advantages such as simple structure, high precision and high transmission ratio. Hence harmonic drive is widely used in industrial fields including aircraft industry, aerospace industry, ...学位：工学硕士院系专业：航空航天学院_航空宇航推进理论与工程学号：3202013115287

关于拟遗传代数的Kazhdan-Lusztig 理论

用同调代数的方法, 研究拟遗传代数与其反代数, 与其标准商代数之间关于Kazhdan-Lusztig 理论的性质. 证明了: 设拟遗传代数(A , +) 有相应于长度函数l 的Kazhdan-Luszig 理论, 则它的反代数(A op , +) 也有相应于l 的Kazhdan-Lusztig 理论, 它的标准商代数(A öE iA ,+ i) 有相应于诱导函数l′的Kazhdan-L usztig 理论.国家自然科学基金资助 教育部优秀青年教师基金 福建省优秀留学回国人员基

七种捕食性鱼类对中华绒螯蟹幼蟹捕食风险的评估

分别以鲤、鳜、斑点叉尾、黄颡鱼、瓦氏黄颡鱼、大口鲇和乌鳢作为捕食者,以中华绒螯蟹幼蟹作为猎物,在室内水泥池(2.4 m3)进行捕食试验。以日捕获率和日摄食率为指标,评估这些鱼类对幼蟹的捕食作用和危害程度,为提高湖泊幼蟹放流效果、建立蟹—鱼复合的优质高效养殖模式提供科学依据。在幼蟹完全暴露的条件下,经过多次(至少9次)重复的试验(短期1d和长期7d),鳜对不同大小的硬壳和软壳(刚蜕壳的)幼蟹没有任何捕食作用;黄颡鱼对硬壳和软壳幼蟹也没有捕食作用,但还需做进一步观察;虽然鲤、瓦氏黄颡鱼对硬壳蟹的捕获率低,但对软壳的幼蟹有较大的危害性,对幼蟹的日摄食率分别为0.070%、0.012%;大口鲇、斑点叉尾、乌鳢对幼蟹具有较强的捕食能力,对幼蟹的日摄食率分别为0.122%、0.188%和0.284%。根据这些研究结果,可以建议:(1)在池塘和湖泊河蟹养殖中,完全可以将鳜作为套养或混养对象,以期提高养殖效益;(2)在河蟹放养的湖泊,需要抑制乌鳢和大口鲇种群,适当减少鲤和瓦氏黄颡鱼丰度,以期减少这些鱼类的捕食作用,提高幼蟹存活率;(3)在河蟹养殖池塘,不能放养乌鳢、大口鲇、斑点叉尾、瓦氏黄颡鱼和鲤

RXRα acts as a carrier for TR3 nuclear export in a 9-cis retinoic acid-dependent manner in gastric cancer cells

Retinoid X receptor (RXR) plays a crucial role in the cross talk between retinoid receptors and other hormone receptors including the orphan receptor TR3, forming different heterodimers that transduce diverse steroid/thyroid hormone signaling. Here we show that RXRalpha exhibits nucleocytoplasmic shuttling in MGC80-3 gastric cancer cells and that RXRalpha, shuttling is energy-dependent through a nuclear pore complex (NPC)mediated pathway for its import and an intact DNA binding domain-mediated pathway for its export. In the presence of its ligand 9-cis retinoic acid, RXRalpha was almost exclusively located in the cytoplasm. More importantly, we also show that RXRalpha. acts as a carrier to assist translocation of TR3, which plays an important role in apoptosis. Both RXRalpha and TR3 colocalized in the nucleus; however, upon stimulation by 9-cis retinoic acid they cotranslocated to the cytoplasm and then localized in the mitochondria. TR3 export depends on RXRalpha as in living cells GFP-TR3 alone did not result in export from the nucleus even in the presence of 9-cis retinoic acid, whereas GFP-TR3 cotransfected with RXRalpha was exported out of the nucleus in response to 9-cis retinoic acid. Moreover, specific reduction of RXRalpha levels caused by anti-sense RXRalpha abolished TR3 nuclear export. In contrast, specific knockdown of TR3 by antisense-TR3 or TR3-siRNA did not affect RXRalpha shuttling. These results indicate that RXRalpha is responsible for TR3 nucleocytoplasmic translocation, which is facilitated by the RXRalpha ligand 9-cis retinoic acid. In addition, mitochondrial TR3, but not RXRalpha was critical for apoptosis, as TR3 mutants that were distributed in the mitochondria induced apoptosis in the presence or absence of 9-cis retinoic acid. These data reveal a novel aspect of RXRalpha function, in which it acts as a carrier for nucleocytoplasmic translocation of orphan receptors

Effect of edaravone on cerebral edema and lipid peroxidation in rats of cerebral hemorrhage

目的探讨依达拉奉对大鼠脑出血后脑水肿及脑组织氧化损害的影响。方法选取健康雄性清洁的SD大鼠150只,随机分为假手术组、对照组、治疗组,每组50只,分别于术后0.5、1、2、3、4 d 5个时间点对每组中不同10只进行观察。采用立体定向自体血注入法制作大鼠脑出血模型,检测3组并比较各时间点血肿周围脑组织含水量变化、丙二醛(MDA)含量变化及超氧化物歧化酶活力情况。结果假手术组和治疗组术后1~4 d各时间点血肿周围脑组织含水量均显著低于对照组(P<0.05);对照组在术后2d脑水肿达到最高值;假手术组、治疗组术后0.5~4 d各时间点血肿周围脑组织MDA含量均显著低于对照组(P<0.05);假手术组、治疗组术后0.5~4 d各时间点血肿周围脑组织SOD活力均显著高于对照组(P<0.05)。结论依达拉奉能明显改善脑出血大鼠血肿周围脑组织水肿,减轻脑出血大鼠脑组织氧化损害。Objective To discusse the effect of edaravone for brain tissue oxidative damage after cerebral hemorrhage through observing the hemorrhage rats back edema and the change of lipid peroxidation. Methods 150 healthy and clean male SD rats,were randomly divided to sham operation group,control group,treatment group,50 cases in each group,each group choose five time points,post operative 0. 5 day,1 day,2 days,3 days,4 days,observed different 10 rats at each time point. The intracerebral hemorrhage model was made by using the method of stereotactic autologous blood injection. The changes of water content,the content of MDA and the activity of superoxide dismutase were monitored in three groups at different time points. Results Surrounding the hematoma in the brain tissue around the hematoma after intracerebral hemorrhage in sham operation group,treatment group of postoperative day 1-4 days each time hematoma surrounding brain tissue water content was significantly lower than that in control group( P <0. 05); Control group after 2 days of cerebral edema and reached the highest value; Sham operation group and treatment group of postoperative half of the day to 4 days each time the content of MDA were significantly lower than in control group( P < 0. 05); Sham operation group and treatment group postoperative half of the day to 4 days each time the brain tissue SOD activity was significantly higher than control group( P < 0. 05). Conclusion The effect is obvious that edaravone for cerebral hemorrhage rats brain tissue around hematoma edema,reduce cerebral hemorrhage rat tissue oxidative damage.福建省医学创新课题项目(2015-1-85

中国各地区人口特征和房价波动的动态关系

本文分析了人口特征、金融市场和房地产市场三者的相互影响机制,基于2002-2015年我国大陆31个省、自治区、直辖市的年度数据,建立了面板平滑转换模型,将人口密度作为异质变量构建计量模型,研究房地产市场的非线性影响因素,及各地区人口特征对房价波动的影响机制。实证结果表明:人均GDP对房价的影响随人口密度增加呈现非线性提升效应;人口密度小的地区,M2存量对房地产价格有正向影响;当人口密度较小时,地区中老年人口占比越大,房价下降的可能越大,反映了房地产\"年轻人推动房价上涨\"的现象,但是极少人口密度比较大城市例外