144 research outputs found

    EFFECT OF ALEXANDRIUM TAMARENSE ON POPULATION DYNAMICS OF BRACHIONUS PLICATILIS

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    采用单个体培养方法研究有毒赤潮藻塔玛亚历山大藻(Alexandriumtamarense)的细胞再悬浮液、细胞破碎液及其与亚心形扁藻(Platymonassubcordiformis)的混合藻液对褶皱臂尾轮虫(Brachionusplicatilis)实验种群动态的影响.结果表明,塔玛亚历山大藻及其细胞破碎液延缓了褶皱臂尾轮虫的生长发育,使轮虫的生殖前期延长,生殖期及寿命缩短,特定年龄出生率降低,产卵量减少,从而导致轮虫生殖力下降,种群增长受阻.其中以塔玛亚历山大藻细胞再悬浮液对轮虫的影响最为显著,rm仅为0.1832d-1.图1表2参18The effect of a toxic dinoflagellate, Alexandrium tamarense (strain ATHK) on the experimental population dynamics of the individual cultured rotifer, Brachionus plicatilis was studied. The experiment is divided into four treatments by culturing the rotifer in sand-filtered seawater with Platymonas subcordiformis as control;in sand-filtered seawater with A. tamarense cells as re-suspended treatment; in sand-filtered seawater with P. subcordiformis and broken A. tamarense as broken cells treatment; and in sand-filtered seawater with P. subcordiformis and A. tamarense as mixed cells treatment. The results showed that A. tamarense and its broken cells retarded the growth and development of B. plicatilis by prolonging its reproductive prophase, shortening its reproduction and life expectancy, reducing its natality in certain ages and decreasing the amount of spawned eggs. In all cases, the re-suspended A. tamarense cells produced the most significant adverse effect on the rotifer with the least r_ m (0.183 2 d -1 ). Fig 1, Tab 2, Ref 18教育部骨干教师基金项目;; 集美大学基金项目(C50209)资助~

    普鲁兰基肿瘤靶向性纳米粒子的制备、稳定性和体外释放

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    背景:普鲁兰多糖以其独特的优点在纳米递药系统领域受到越来越多的关注,但是,以普鲁兰多糖为材料进行改性制备的肿瘤靶向的纳米药物载体仍有待进一步研究与开发。目的:观察纳米粒子和载药纳米粒子的体外稳定性及所包载药物的释放特征,初步评价其作为纳米药物载体的潜力。方法:应用透析法制备乙酰普鲁兰叶酸偶合体纳米粒子,以表阿霉素为模型药物,制备乙酰普鲁兰叶酸偶合体/表阿霉素载药纳米粒子(FPA/EPI),应用储存法考察其稳定性,应用透析袋法观测体外释放特征。结果与结论:乙酰普鲁兰叶酸偶合体纳米粒子和FPA/EPI的粒径分别为(204.2±10.9)nm和(273.4±11.0)nm,在蒸馏水和体积分数10%胎牛血清中表面电位均较低,乙酰普鲁兰叶酸偶合体纳米粒子在水溶液中粒径1年内未见显著改变。载药纳米粒子对所包载的药物表阿霉素进行很好地释放,pH5.0磷酸盐缓冲液中释放速度明显高于pH7.4;乙酰普鲁兰叶酸偶合体纳米粒子和FPA/EPI制备容易,稳定性好,初步说明了两种粒子可望成为新型肿瘤靶向药物递药系统

    中国红树林的害虫种类及其综合防治

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    通过对文献资料的统计,共发现中国红树林害虫128种,隶属2纲7目55科,其中以鳞翅目、半翅目昆虫为主,分别占总数的52.4%和26.6%。危害无瓣海桑Sonneratia apetala、桐花树Aegiceras corniculatum、秋茄Kandelia obovata、白骨壤Avicennia marina、海桑Sonneratia caseolaris的害虫分别有45种、45种、37种、32种和20种。广东红树林害虫种类最多,共计95种,广西其次,有49种。鳞翅目害虫多以幼虫取食为害,主要包括海榄雌瘤斑螟Acrobasis sp.、桐花树毛颚小卷蛾Lasiognatha cellifera Meyrick、棉古毒蛾Orgyia postica Walker、蜡彩袋蛾Chalia larminati Heylaerts、丽绿刺蛾Latoia lepida Cramer等;半翅目害虫以幼虫、成虫吸食植物汁液为害,主要有考氏白盾蚧Pseudaulacaspis cockerelli (Cooley)、广翅蜡蝉Ricania sp.等。在虫害的综合防治中,以生物防治为主,即利用寄生蜂、苏云金杆菌、白僵菌、捕食性天敌和生物制剂等进行防治,结合物理防治方法可以提升防治效果。此外,对红树林虫害暴发的原因和机制也进行了探讨分析

    福建省力学学科发展报告

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    介绍了福建省力学主要学科的研究进展、主要研究成果及在国民经济建设中的主要应用情况与存在的问题,简单介绍了福建力学学科整体在国内外的地位,展望了我省力学学科发展方向及其在海西建设中的作用

    老年人备灾现状及应对策略的研究进展

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    阐述国内外老年人的备灾现状,老年人应对灾害能力下降,备灾能力较低,应对灾害相关知识和教育缺乏,慢性疾病影响其备灾状态,其获取备灾信息途径和方式单一。提出需建立社区备灾组织、提供..

    一种利用荧光检测微囊藻细胞DNA损伤的方法

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    本发明公开一种利用荧光检测微囊藻细胞DNA损伤的方法,其步骤:(a)将待检测样品和对照样品均分别平均分成三小组;(b)去除细胞角质鞘:离心收集样品中藻细胞并重悬浮于SE缓冲液中洗涤;(c)细胞裂解:将细胞分别重悬浮于Lysis裂解液,加入蛋白酶K和十二烷基磺酸钠,使细胞裂解;(d)细胞DNA链解旋:改变pH,使T、P和B样品在不同条件下解旋;(e)染色:分别向以上T、P和B样品中加入Hoechest 33258染色;(f)荧光测定:离心后于荧光检测器中检测上清液的荧光强度;(g)结果计算:根据待测样品和对照样品中T、B和P样品的荧光来计算DNA链的断裂水平。本方法易于掌握,并且灵敏度高,DNA链上单个断裂位点即可检测到。</p

    Preparations and Electrochemical Properties of BC/CoNi2S4@PPy Flexible Composites for Supercapacitors

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    本文采用溶剂热、原位聚合和真空抽滤相结合的方法制备了用于超级电容器的细菌纤维素/镍钴硫化物/聚吡咯(BC/CoNi2S4@PPy)柔性电极材料,通过X射线衍射、场发射扫描电镜、红外光谱、氮气吸脱附、拉伸强度和接触角表征了材料的形貌结构、组成、机械性能和亲水性,并采用循环伏安法和恒电流充放电测试了复合材料的电化学性能。结果表明,表面含氧官能团丰富的BC纤维网络结构对氧化还原活性物质CoNi2S4的生长和导电聚合物PPy的分布具有引导作用,CoNi2S4均匀分布在BC网络中,且PPy均匀包覆在BC纤维和CoNi2S4纳米球表面构成具有丰富孔隙结构的三维导电网络,使得该复合材料具有较好的机械性(抗拉强度达28.0±0.1 MPa)、亲水性(对6 mol·L-1 KOH的瞬间接触角为43.6°)及良好的导电性。该电极材料在1 A·g-1下比电容高达2670 F·g-1,充放电循环10000次后比电容的保持率为82.73%,且经1000次反复弯曲后电化学性能保持不变。此外,将其与活性炭组成的非对称超级电容器,在1 A·g-1下比电容为1428 F·g-1,最高能量密度和功率密度分别达49.8 Wh·kg-1和741.8 W·kg-1。Flexible supercapacitor is one of the most promising energy storage devices for portable and wearable electronic products due to its advantages of high power density, fast charging and long cycle life. Therefore, self-supporting flexible electrode materials with high performance have attained more and more attention both in academia and in industry recently. In this work, using bacterial cellulose (BC) as a flexible substrate, the bacterial cellulose/nickel-cobalt sulfide@polypyrrole (BC/CoNi2S4@PPy) flexible composites with three-dimensional porous network and good conductivity were prepared by a combined solvothermal-in-situ polymerization-vacuum filtration method. The samples were characterized by X-ray diffraction, field emission scanning electron microscopy, Fourier transform infrared spectrometry, N2 physisorption, tensile strength and contact angle measurements. Their electrochemical performances were tested by cyclic voltammetry, galvanostatic charge/discharge testing and electrochemical impedance spectroscopy. The results show that the three-dimensional porous network of BC fibers with rich oxygen-containing surface groups play a guiding role in the growth of the redox active material CoNi2S4 and the distribution of conductive polymer PPy, resulting in uniformly distributed CoNi2S4 nanospheres in the network of BC fibers, both coated evenly with a layer of conductive PPy. The resulting BC/CoNi2S4@PPy composites, a three-dimensional conductive network with high porosity, displayed good mechanical property (tensile strength up to 28.0±0.1 MPa), hydrophilicity (the instantaneous contact angle in 6 mol·L-1 KOH is 43.6°), as well as excellent electrochemical performance. The specific capacitance of the flexible BC/CoNi2S4@PPy was 2670 F·g-1 at 1 A·g-1 in a three-electrode system, and retained 82.7% after 10000 charge and discharge cycles. In addition, the electrochemical performance remained unchanged after 1000 times of repeated bending. In an asymmetric supercapacitor composed of BC/CoNi2S4@PPy and activated carbon, the area specific capacitance was 1428 F·g-1 at 1 A·g-1. The asymmetric supercapacitor achieved the maximum energy density of 49.8 Wh·kg-1 and power density of 741.8 W·kg-1.国家重点基础研究发展计划项目(2014CB239702);国家自然科学基金项目(21676082)通讯作者:周静红E-mail:[email protected]:Jing-HongZhouE-mail:[email protected]华东理工大学化工学院,上海 200237School of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, Chin

    1978~2008年中国湿地类型变化

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    分别基于美国陆地卫星(Landsat MSS/TM/ETM+)和中巴资源卫星(CBERS-02B)影像数据,以人工目视解译为主,完成了中国1978~2008年4期(基准年分别为1978,1990,2000和2008年)湿地遥感制图,并进行了大量的室内外验证.在此基础上,对我国湿地现状及近30年来湿地变化进行了初步分析,得到以下主要结论:(ⅰ)截止2008年,中国湿地面积约为324097km2,其中以内陆沼泽(35%)和湖泊湿地(26%)为主.(ⅱ)1978~2008年,中国湿地面积减少了约33%,而人工湿地增加了约122%.过去30年里湿地减少的速度大幅降低,由最初5523km2/a(1978~1990年)降为831km2/a(2000~2008年).(ⅲ)减少的自然湿地(包括滨海湿地和内陆湿地),其类型变化由湿地向非湿地转化的比例逐渐降低.初期(1978~1990年)几乎全部(98%)转换为非湿地;在1990~2000年间减少的自然湿地约有86%转化为非湿地,而在2000~2008年,这一比例下降为77%.(ⅳ)气候变化和农业活动是中国湿地变化的主要驱动因素,湿地变化在中国分为三大不同特征区域,即西部三省/自治区(西藏、新疆和青海)、北部两省/自治区(黑龙江和内蒙古)和其他省市区.其中西部区域尤其是青藏高原,湿地变化的驱动因子以气候增温为主;新疆湿地由于气候增温和农业活动共同作用造成变化不大.北部省/自治区的湿地变化则主要由农业活动引起;而其他省市区的湿地变化几乎完全受控于人类的农业经济活动

    Effects of toxic dinoflagellate Alexandrium tamarense on two unicellular algae

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    本文研究了塔玛亚历山大藻(ATCI01品系)细胞再悬浮液、细胞破碎液、上清液、藻壳液、过滤液对亚心形扁藻悬浮性的影响,以及塔玛亚历山大藻与亚心形扁藻和小球藻之间的相互作用.结果发现,含有塔玛亚历山大藻成分的实验溶液均能在短时间内造成亚心形扁藻失去鞭毛形成胞囊而下沉,其中再悬浮液的影响最大.但亚心形扁藻受到的这种不利影响,一般在一周后即开始恢复.小球藻生长几乎不受塔玛亚历山大藻的影响,混合培养水体中的小球藻生长曲线与单种培养的几乎一致,其悬浮性也无明显改变.与亚心形扁藻进行混合培养的塔玛亚历山大藻(ATCI01品系和ATHK品系)均表现出培养前期增殖曲线与单种培养类似,但在一周后混合培养的塔玛亚历山大藻的增殖曲线开始下降,这一时间恰好与扁藻细胞密度开始增加的时间相一致.而与小球藻的混合培养的塔玛亚历山大藻在培养前期与单种培养一致,后期则种群数量有所下降.The effects of a toxic dinoflagellate, Alexandrium tamarense (strain ATCI01) on the suspension of Platymonas subcordiformis with its re-suspended cells, broken cells, cell contents, cellular fragments and free-cell medium were investigated, and the interaction in the growth between Alexandrium tamarense and Platymonas subcordiformis or Chlorella sp. was also studied. The results showed that cells of Platymonas subcordiformis cultured in the various treatments above-mentioned lost the flagellums, formed cystsand settled on the bottom of the flasks in short time, and the most obvious effect was observed in the re-suspended cells. However, the negative impact lasted onlya few days, and the cells of Platymonas subcordiformis recovered after a week. There was no obvious change in the growth curves for the suspension of Chlorella sp. whether it was in uni-culture or mixed-culture with Alexandriumtamarense. Moreover, the growth curves of ATCI01 and ATHK in uni-culture or (mixed-culture) with (Platymonas) (subcordiformis) were similar in prophase, but the curves of A. tamarense in mixed-culture declined a week later when the cell density of P.subcordiformis increased gradually. The same phenomina occurred in the A.tamarense culture mixed with Chlorella sp.教育部骨干教师基金资助项目;; 集美大学基金资助项目(C50209

    寻求内源发展:中国西部的民族与文化

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    本书通过对中国藏区的发展、新疆与甘肃的畜牧业综合发展和青海省人与自然和谐发展的三个项目评估报告,结合项目区各少数民族社会和文化现状,探索西部内源发展和文化资本彰显之路
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