Preparation,identification and application of monoclonal antibody against urease subuint B of Helicobacter pylori

目的制备抗幽门螺杆菌(HP)的单克隆抗体(MAb),并对该HP MAb进行分析鉴定,建立一种检测患者由于HP感染而在血清中产生HP抗体的竞争ElISA检测方法。方法用灭活的HP免疫bAlb/C小鼠,通过杂交瘤技术制备HP MAb。我们采用HP混合蛋白包括毒素相关蛋白A(CAgA)、空泡毒素A(VACA)和尿素酶以及灭活的HP菌体筛选阳性杂交瘤细胞株,用ElISA和WESTErn blOT等技术对所获得的HP MAb进行鉴定。利用辣根过氧化物酶(HrP)标记所筛选的HP MAb来建立一个可检测患者血清中HP抗体的竞争ElISA。结果通过大规模的杂交瘤筛选,我们选择了1株将其命名为C3 HP MAb,其抗体亚型为Igg2A,腹水效价可达1x107。WESTErn blOT法、ElISA和质谱检测结果显示,该C3 HP MAb能特异地识别HP的尿素酶b亚单位。用这个C3 HP MAb,我们建立了一种可检测患者血清中HP抗体的竞争ElISA。结论成功获得一种可以特异识别HP尿素酶b亚单位的MAb,建立了一种可检测患者血清中HP抗体的竞争ElISA。Objective To prepare and characterize the monoclonal antibody(mAb) against Helicobacter pylori(Hp)and establish a competitive ELISA used for detection of Hp antibodies in the sera of Hp-infected patients.Methods BALB /c mice were immunized with inactivated Hp to generate Hp mAb using the hybridoma technology.Hp mixed proteins including cytotoxin-associated gene A(CagA),vacuolating cytotoxin A(VacA) and urease,as well as inactivated Hp were applied to screen positive hybridoma.Selected Hp mAb was analyzed and characterized with ELISA and Western blotting,and then labeled with horseradish peroxidase( HRP) for establishing a competitive ELISA to detect Hp antibodies in the sera of Hp-infected patients.Results One Hp mAb named as C3 was selected after screening large amount of hybridoma,and the C3 Hp mAb was special for IgG2 a subtype with the affinity titer of 1 × 107.Western blotting,ELISA and mass spectrum analysis indicated that the C3 Hp mAb could recognize Hp urease subunit B specifically.Using the C3 Hp mAb,we developed a competitive ELISA which could be used to detect Hp antibodies in the sera of Hp-infected patients.Conclusion We successfully obtained one mAb that could specifically recognize Hp urease subunit B and developed a competitive ELISA using the Hp mAb.福建省科技重点项目(2011Y0050); 厦门市科技计划项目(3502Z20123009); 国家基础科学人才培养基金(J1310027

高层混合结构中楼盖刚度对结构受力性能的影响分析

通过楼板将两种变形特性完全不同的结构体系连接而成混合结构,在受力性能上与框架结构、筒体结构既具有一定的联系,又有本质的区别。本文以一30层外部为钢框架、内部为混凝土核心筒组成的混合结构为例,采用通用有限元程序SAP2000分别计算了钢框架结构、混凝土筒体结构及混合结构,比较了三种结构体系的受力性能;通过调整楼板型式和板厚,探讨了楼板刚度对混合结构受力性能的影响。结果表明,采用混合结构使得结构变形、内力沿高度方向趋于均匀,受力性能得以改善;采用梁板体系能有效地减轻剪力滞后;随着楼板刚度的增加,结构高阶自振周期增大,侧移减少,层间剪力、楼层弯矩及总钢框架承担剪力、弯矩增加;而钢框架承担总剪力、弯矩百分比减少

化学工程与工艺的自动化发展趋势

化学工业的开展能够运用于我国的许多范畴，比如农业工业和现代化制造业，它的发展可以对工业的生产工艺进行一定程度的提升，并且加大了我国的国防力量，为我国的国家安全提供了重要保证，使农业生产的发展也得到了提升，促进了高新技术产业的发展，使人们的生活程度与质量持续进步。化学工程与工艺是化学这一学科中十分重要的组成成分，在化学工业的发展过程中具备非常重要的促成意义。</jats:p

The Fatigue Damage Research of Bogie Frame Based on the FKM-Guideline

The fatigue damage of a freight car bogie frame was calculated according to the FKM-Guideline and the commercial software MSC.Fatigue respectively. From the comparison, it cought be concluded that the result of FKM-Guideline was bigger than that of MSC. Fatigue for welded components, while for non-welded components, the result was opposite. By analyzing the parameters of the FKMGuideline, the fatigue life assessment of bogie frames based on the FKM-Guideline has certain practical value

松鸦的冬季生态

松鸦（Garrulus glandarius）在我国虽然为留鸟,但对其生态和生物学研究甚少,仅见郑作新等（1963、1973、1976）有所报道。系统的研究报道迄今尚未见及。为此我们干1982年10—12月和翌年1—3月、10—12月和1984年的1—3月,在山西省庞泉沟自然保护区,对松鸦的冬季生态初步进行了观察,现将结果整理成文