Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor

[目的]建立稳定表达融合EGFP的人新生儿Fc受体（h FcRn）的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5%,流式单细胞分选后得到纯阳性细胞;荧光共定位及Western Blot均检测到h FcRn的完整表达;流式分析表明细胞株上的h FcRn与Ig G存在p H依赖性结合。[结论]成功获得稳定表达具有生物活性的可视化h FcRn的MDCK细胞株。[ Objective] To establish MDCK cell line stably expressing EGFP- human neonatal Fc receptor（hFcRn） fusion protein. [ Methods ] The lentiviral expression vector for EGFP - hFcRn fusion protein was constructed. Generating by co - transfection of four -plasmids into HEK 293T cells ,the lentivirus particles were used to infect MDCK cell line. EGFP positive single cell was obtained by FACS, and then FcRn expression was identified by fluorescence co -location with EGFP - β2m and confirmed by Western Blot. Flow cytometry was used to detect binding activity of hFcRn and human IgG. [ Results ] DNA se- quencing demonstrated that the lentivirus vector pEGFP - FcRn was constructed successfully. The percentage of EGFP - posi- tive ceils was about 26.5% after infection. Expression of the complete protein was detected through fluorescence co - location and Western Blot, respectively. Flow cytometry analysis showed that the cell lines could pH - dependently capture human IgG. [ Conclusion] MDCK cell line stably expressing functional visualable hFcRn was established.基金项目：国家自然科学基金项目（“结构生物学指导的HBV治疗性抗体人源化及其关键技术研究”,No.31600748;“抗呼吸道合胞病毒高中和活性抗体的保护机制研究”,No.81401668;“基于广谱中和单抗的通用型流感疫苗设计及其结构基础研究”,No.31670934

Polyphenol Contents in Eight Fruits and Their Antioxidant Activities

[中文摘要]测定了苹果、石榴、橄榄、芒果、香蕉、菠萝、葡萄和龙眼的壳、肉及核中的多酚含量及其抗氧化性。以70%丙酮(v/v)为提取溶剂,室温下超声波辅助浸提样品后得到提取液。采用普鲁士兰(Prussian blue)法测定了提取液中多酚和单宁的含量,利用FRAP法测定其抗氧化性。结果表明:提取液中多酚和单宁的含量均与其抗氧化性成正相关关系;石榴、橄榄、芒果、葡萄和龙眼等水果的壳与核有望成为天然抗氧化剂的新来源。[英文摘要]The contents o f polyphenols of peel pulp and seed fractions of apple, pome granate, olive, mango, banana, pineapple, grape and long an and their to talantioxidant ac tiv ities were studied. A fter ultrasound-assisted extraction w ith 70% aqueous acetone at room temperature, polypheno ls and tann ins in the p lant ce lls w ere separated and their contents were determ ined by Prussian blue assay. Antioxidant activities of the aqueous acetone extracts were evaluated by Ferric Reducing /Antioxidant Power (FRAP) assay. A positive linear correlation between antioxidant activities and phenolic contents was observed. The resu lts show ed that the fru it pee l and seeds o f pom egranate, olive, mango, grape and longan could be conside red as potentia l sources o f antioxidants rather than just discarded as waste

股权结构、多元化战略与资本结构—基于代理理论的解释及经验证据

由厦门大学会计发展研究中心提供的第五届会计与财务问题国际研讨会——当代管理会计新发展论文集中的第二部分：战略管理会计与平衡记分卡4本文以我国上市公司为样本，验证在我国特殊的股权结构下，多元化经营是否对资本结构的决策产生影响。我们发现：资本结构与国有股所占的比例显著正相关；资本结构与法人股所占的比例负相关（不显著）；资本结构与管理层的持股比例之间是U型的非线性；资本结构与股权集中度显著正相关。多元化与资本结构之间显著负相关。多元化程度与管理层持股比例之间显著正相关，多元化是经理层获取私人利益的手段。但不是增加在职消费的手段。全部样本公司，资本结构与股权集中度显著负相关。在多元化公司，资本结构与股权集中度显著正相关。多元化影响股权结构与资本结构之间的关系。多元化经营的条件下，资本结构与管理层持股比例之间显著正相关

基于WWW方式的优势区相图计算

优势区相图的早期版本是用 Ｆｏｒｔｒａｎ 语言开发的， 由于历史原因， 只能在 Ｄ Ｏ Ｓ 操作平台单机运行， 界面不友好， 使用不便， 限制了它的推广和应用。本文介绍了应用 Ｉ Ｓ Ａ Ｐ Ｉ、 Ｊａｖａ 等技术， 以 Ｗｉｎｄｏｗｓ ３２ 位操作系统为工作平台， 采用 Ｍｉｃｒｏｓｏｆｔ Ｖｉｓｕａｌ Ｃ ＋＋ 结合 Ｖｉｓｕａｌ Ｂａｓｉｃ 、 Ｖｉｓｕａｌ Ｊ＋ ＋ 等编程工具， 实现了网络化的优势区相图实时计算服务。本文所探讨的技术路线可以为一般的网络化科学计算提供参考

基于WWW方式的优势区相图计算

优势区相图的早期版本是用Ｆｏｒｔｒａｎ语言开发，由于历史原因，只能在ＤＯＳ操作平台单机运行，界面不友好，使用不便，限制了它的推广和应用。本文介绍了应用ＩＳＡＰＩ，Ｊａｖａ等技术，以Ｗｉｎｄｏｗｓ３２位操作系统为工作平台，采用Ｍｉｃｒｏｓｏｆｔ Ｖｉｓｕａｌ Ｃ＋＋结合Ｖｉｓｕａｌ Ｂａｓｉｃ，Ｖｉｓｕａｌ Ｊ＋＋等编程工具，实现了网络化的优势区相图实时计算服务

花尾榛鸡的人工育雏研究

花尾榛鸡的人工育雏研究葛东宁，朴仁珠，洪运泰，高志远（黑龙江省野生动物研究所）花尾感鸡（Ｂｏ，ｌａ。ｂｏｎａ。ｉ’ｌ）为松鸡科鸟，分布于我国东北林区。近年来数量锐减，已被列为国家１级保护动物。有关花尾滚鸡的育雏研究国内外曾有报导(杨伯然１９８４，Ｈａ..

In-situ TEM construction of tunable carbon nanotube resonators

提出了1种基于电子束致沉积无定形碳技术的可控调节碳纳米管谐振器频率的新方法,利用原位透射电镜技术,通过控制电子束斑的位置、大小和沉积时间,精确控制所沉积无定形碳在碳纳米管悬臂梁上的位置和质量。实验结果表明,通过在碳纳米管悬臂梁顶端沉积碳纳米颗粒可大幅度调节碳纳米管的共振频率,实现谐振频率的粗调模式,调节幅度可超过50%;若将沉积位置远离碳纳米管悬臂梁顶端,则可实现碳纳米管谐振器共振频率的精细调节,调节精度可达到2%。以实验探讨了Rayleigh-Ritz理论对于计算碳纳米管谐振器称量质量的适用性,证明所称量的纳米颗粒的质量越小,计算结果的准确性越高。To tune the frequency of carbon nanotube （CNT） resonators in a highly controlled manner, anovel approach based on e-lectron beam induced deposition of amorphous carbon was proposed. Due to the extremely tiny size of CNTs and the difficulty in controlling the CNT resonator frequency, in-situ transmission electron microscopy was utilized to precisely control the location and mass of the deposited carbon particles along the CNT cantilever by choosing the sites and spot size of the electron beam, as well as the deposition time. Experimental results demonstrate that coarse frequency tuning can be achieved by depositing amor-phous carbon nanoparticles at the tip of the carbon nano tube cantilever, which leads to a large frequency shift range, exceeding 50%. If the carbon deposition is controlled at the positions far away from the CNT tip, tuning precision can be greatly improved, achieving fine tuning of frequency with a precision of 2%. Furthermore, the validity of the Rayleigh-Ritz theory on the mass cal-culation of this CNT nanobalance is experimentally addressed, and it is verified that the lower measured mass leads to the better accuracy of the calculations.高等学校博士学科点专项科研基金资助项目（20130121120009）;国家自然科学基金资助项目（61471307

调水调沙工程长期实施对黄河口近岸沉积物粒度分布与黏土矿物组成特征的影响

基于对2013年10月获取的黄河口近岸海域28个沉积物样品的激光粒度分析和X-射线衍射(XRD)分析,探讨了调水调沙工程长期实施对沉积物粒度分布以及蒙皂石、 伊利石、 高岭石、 绿泥石这4种矿物组成特征的影响. 结果表明, 2002年调水调沙工程实施以来,黄河口近岸海域的沉积物粒度尽管在砂含量上(平均约占23.5%)较渤海海域和黄河沉积物高,但仍主要以粉砂为主(平均约占59.1%),黏土组分相对较少(平均约占17.4%). 黄河口近岸海域的黏土矿物组成与黄河沉积物的组成相近,但较调水调沙工程实施前存在较大差异,其不同组分含量整体表现为伊利石>蒙皂石>绿泥石>高岭石. 研究发现,相对渤海海域大范围的研究,对黄河口近岸海域的局部研究更能揭示调水调沙工程长期实施对河口沉积环境的影响