Studies on the inhibitory mechanism of Crocodile choline on cholangiocarcinoma cells and Chronic toxicity on rats

目前，中医药中动物药对恶性肿瘤的作用受到人们越来越多的关注，由于其疗效显著、治疗后不易复发、毒副作用小等特点，在临床上得到广泛的应用。其中动物胆汁的抗肿瘤作用在国内外都受到了极大的关注。 本论文以体外培养的人胆管癌Mz-ChA-1细胞为对象，通过一系列的分离纯化手段从暹罗鳄胆汁中提取到获得具有抑制胆管癌细胞生长的活性物质鳄胆素，并进一步探讨其抑制胆管癌细胞生长的作用机理。应用普通光学及荧光显微镜观察鳄胆素对Mz-ChA-1细胞形态学的影响；PI单染检测细胞周期变化；AnnexinV-FITC/PI双染法检测经鳄胆素处理后细胞的凋亡情况；流式细胞术检测胞内ROS的积累情况和膜电位的变化；Wes...Nowadays, many people are paying more and more attention to anti-tumor of Chinese medicine including animal medicine, due to its significant effect, difficult to recur after treatment, small toxic side effect and other characteristics, are widely used in clinic. In the present study, the cultured human cholangiocarcinoma cell line Mz-ChA-1 in vitro was used, an active compound which could inhibit...学位：理学博士院系专业：生命科学学院生物学系_细胞生物学专业学号：2172009015357

Expression and Localization of Nucleophosmin During Curcumin-induced Apoptosis in the Immortalized Human Epithelial HaCaT Cells

该文以姜黄素诱导处理前后的人永生化表皮HACAT细胞为研究对象,对核仁磷酸蛋白(nuClEOPHOSMIn,nPM)在核基质中存在、分布及其与凋亡相关基因产物在姜黄素处理前后HACAT细胞中的共定位关系进行观察研究。WESTErn blOT结果显示,nPM存在于人永生化表皮HACAT细胞核基质蛋白组分中,并在姜黄素处理后的细胞核基质中表达下调,免疫荧光显微镜观察显示,nPM定位在核基质上,经姜黄素处理后出现分布位置与表达水平的变化,激光共聚焦显微镜观察可见nPM与HACAT细胞中凋亡相关基因bAX、bCl-2、MTP53和rb基因产物均存在共定位关系,但在姜黄素处理后细胞中其共定位分布区域出现变化。研究结果证实nPM是一种核基质蛋白,定位于核基质上,nPM在HACAT细胞诱导凋亡过程中的表达分布及其与凋亡相关基因产物的共定位现象值得进一步探索和研究。To explore the existence and distribution of nucleophosmin(NPM) in the nuclear matrix and its colocalization with the other apoptosis-related gene products following curcumin treatment in the human epithelial HaCaT cells,the nuclear matrix of HaCaT cells was extracted pre/post curcumin induced apoptosis.Western blot analysis showed that NPM existed in the fractions of nuclear matrix proteins and was down-regulated after curcumin treatment.The immunofluorescence observation revealed that NPM located in the nuclear matrix,curcumin treatment altered its expression level and distribution profile.The colocalization of NPM with the products of apoptosis-related repression genes,including Bax,Bcl-2,mtP53 and Rb,using laser scanning confocal microscopy,were evaluated,and substantial differences were observed following curcumin treatment.The results implied that NPM is a nuclear matrix protein,and the level of its expression and the colocalization with apoptosis-related gene products may play an important role during the apoptosis of HaCaT cells.国家自然科学基金(批准号:30871241)资助的课题~

气象预警信息在城市防灾减灾的重要作用探究

众所周知,在当前社会经济的快速发展下,气象灾害问题影响了农业、工业以及人们的生活,且我国作为全球受气象灾害影响较为严重的国家之一,需要加以重视,做好气象灾害预报预警工作。伴随着科学技术的不断发展,气象灾害预报工作得到有效突破,如何大力开展气象灾害预报报警工作已经成为了当前的关键所在。基于此,本文就气象预警信息在城市防灾减灾的重要作用进行分析,并提出对策。</jats:p