功能聚间苯二胺膜用于免疫传感器分析

以电化学聚合法在石墨电极上获得聚间苯二胺膜（ＰＭＰＤ），并建立了快速、灵敏的膜质量检验方法：辣根过氧化物酶（ＨＲＰ）标记ＰＭＰＤ膜－邻苯二胺（ＯＰＤ）反应法，对聚合、活化等条件作了研究；进一步制成了检测乙肝表面抗原（ＨＢｓＡｇ）和检测鼠免疫蛋白（ＭＩｇＧ）的免疫电极，对血清样品作了检测．结果表明：２．５Ｖ电池电压下，于含０．１２ｍｏｌ／ＬＭＰＤ的１．２ｍｏｌ／ＬＨ２ＳＯ４中聚合２０ｍｉｎ，再以３０ｇ／Ｌ浓度的戊二醛活化４ｈ，所得ＨＲＰ标记电极及免疫电极重现性好；检测ＨＢ－ｓＡｇ和ＭＩｇＧ的线性范围分别为０．１～３．２ｍｇ／Ｌ及０．１～１０ｍｇ／

Synthesis and identification of artificial complete antigen 25-hydroxyvitamin D_3

目的:合成25-羟基维生素d3人工完全抗原,并制备抗25-羟基维生素d3的特异性抗体。方法:将25-羟基维生素d3进行化学修饰加入羧基活性基团,合成具有半抗原结构特征的25-羟基维生素d3-半琥珀酸酯。采用碳二亚胺法,将25-羟基维生素d3-半琥珀酸酯,分别与牛血清白蛋白(bSA)和卵清蛋白(OVA)偶联,合成人工完全抗原25-羟基维生素d3-半琥珀酸酯-bSA和25-羟基维生素d3-半琥珀酸酯-OVA。通过紫外吸收光谱,SdS-PAgE和MAldI-TOf进行偶联鉴定。用25-羟基维生素d3-半琥珀酸酯-bSA免疫小鼠,获得抗25-羟基维生素d3抗体免疫血清。结果:25-羟基维生素d3-半琥珀酸酯与bSA的偶联比为(12±0.16)∶1,免疫小鼠后获得高效价(效价为6.25x10-4)的抗体,且标准品浓度在37.5~600 ng/Ml范围具有显著的竞争性线性关系,检测的灵敏度为37.5 ng/Ml。结论:成功合成了25-羟基维生素d3人工完全抗原,制备出25-羟基维生素d3的抗体且其线性关系显著,灵敏度较高,为进一步研制检测25-羟基维生素d3的试剂盒奠定了基础。AIM: To synthesize the 25-hydroxyvitamin D3 artificial complete antigen and to prepare the specific antibody against 25-hydroxyvitamin D3.METHODS: The active group carboxyl was introduced into 25-hydroxyvitamin D3 and formed 25-hydroxyvitamin D3-hemisuccinate which possessed the structure of the hapten by chemical modification.The EDC method was applied to conjugate 25-hydroxyvitamin D3-hemisuccinate to bovine serum albumin as an artificial immunogen.The coating antigen 25-hydroxyvitamin D3-hemisuccinate-OVA was obtained in the same way.Ultraviolet,SDS-PAGE and MALDI-Tof were used to identify 25-hydroxyvitamin D3-hemisuccinate-BSA.RESULTS: BALB/c mice were immunized with 25-hydroxyvitamin D3-hemisuccinate-BSA to generate the polyclonal antibody of the 25-hydroxyvitamin D3 worth high titer and the immunogen,25-hydroxyvitamin D3-hemisuccinate-BSA,was successfully prepared with coupling ratio(12±0.16)∶1(N=3) coupling.CONCLUSION: The high titer and good sensitivity of anti-25-hydroxyvitamin D3 antibody are produced in sera immunized BALB/c mice,which made it possible to develop a clinical diagnostics for illness

樟树籽仁油的遗传毒性评价

为评价樟树籽仁油的食用安全性，通过细菌回复突变试验、体外哺乳类细胞TK基因突变试验及哺乳动物红细胞微核试验探究樟树籽仁油的致突变和潜在致癌作用。结果表明：樟树籽仁油对组氨酸缺陷型鼠伤寒沙门氏菌TA1535、TA97、TA98、TA100、TA102均无致突变作用；樟树籽仁油未抑制L5178Y小鼠淋巴瘤细胞的生长，对体外哺乳类细胞TK基因无致突变作用；樟树籽仁油未导致小鼠细胞微核率上升，对哺乳动物红细胞无致突变作用，无遗传毒性。综上，樟树籽仁油无细胞致突变和潜在致癌作用，没有遗传毒性，具有一定的食用安全性。To evaluate the edible safety of Cinnamomum camphora seed kernel oil (CCSKO), the mutagenicity and potential carcinogenic effects of CCSKO were investigated by bacterial reverse mutation tests, in vitro mammalian cell TK gene mutation tests, and mammalian erythrocyte micronucleus tests. The results showed that CCSKO had no mutagenic effect on Salmonella typhimurium strains TA1535, TA97, TA98, TA100 and TA102 with histidine deficiency. CCSKO did not inhibit the growth of L5178Y mouse lymphoma cells, and had no mutagenic effect on TK gene in mammalian cells in vitro. CCSKO did not increase the micronucleus rate of cell in mice, had no mutagenic effect on mammalian erythrocytes and had no genotoxicity. To sum up, CCSKO has no mutagenicity and potential carcinogenic effect, no genotoxicity, and has certain edible safety

太原龙山道教石窟第五窟主像身份探析

龙山道教石窟位于山西省太原市西南20公里处的龙山山巅东侧巨型岩体上,为第四批全国重点文物保护单位。石窟开凿于唐、元、明三个时期~([1]),共有9座洞窟65尊像,是我国北方现存最大、保存最完整的纯粹道教题材石窟。由于石窟所处地理位置较为偏僻,且位于龙山山巅,自20世纪80年代以后,始有国内学者对其进行考察和研究,随后在石窟年代、造像风格、内容题材等方面均取得了一定成果~([2])