改良TRIzol法高效提取东北林蛙皮肤总RNA

目的:建立一种从两栖动物皮肤组织中提取高质量总RNA的方法。方法:改良传统TRIzol法,提取皮肤总RNA,紫外分光光度计测定RNA纯度和得率,1.1%琼脂糖凝胶电泳检测其完整性。结果:改良方法提取的总RNA,其A260/A280值在1.8～2.1之间,A260/A230大于2;琼脂糖凝胶电泳显示清晰的28s rRNA和18s rRNA条带,且28s rRNA条带亮度约为18s rRNA的2倍。结论:改良TRIzol法提取的总RNA纯度高、完整性好、杂质少、得率大,易于操作掌握,可以用于相应的分子生物学试验

Cryogenic System of the 10m-10.5kV/1.5kA Three-phase High Temperature Superconducting Cable Plant

one of the main sub-systems of the experimental device, a cryogenic system uses the sub-cooled liquid nitrogen (LN 2) pressurized to cool the HTS cable and its terminations. This cryogenic system has the sub-cooled LN 2 cycle part and the refrigeration part. The former applies a cryogenic pump to force LN 2 through a heat exchanger in the sub-cooler, the three-phase HTS cable cryostats, and a LN 2 gas-liquid separator. The latter is designed for getting the cooling capacity by decompressing LN 2 to sub-atmosphere, and its maximum cooling capacity is about 3.3 kW and the LN 2 consumed is 72 L/h

不同生长基质对白云山国家森林公园苔藓谱系多样性的影响

本研究分析了洛阳白云山国家森林公园1 hm2样地内腐木、岩石、树干和土壤等4种不同生长基质上苔藓植物的谱系多样性,探讨了地形与光照对不同基质苔藓植物谱系多样性的影响,以及不同生境苔藓植物谱系多样性变化的原因。结果显示:样地内土壤生苔藓植物的谱系多样性最高,而树附生苔藓植物的谱系多样性最低;腐木生苔藓植物谱系多样性与坡度、冠层覆盖度和平均叶倾角显著相关;土壤生苔藓植物谱系多样性与总透光比、冠层下散射辐射以及冠层下的总直接辐射相关性显著;岩石生苔藓植物谱系多样性仅与凹凸度显著相关;而树附生苔藓植物的谱系多样性与地形和光照均无显著相关性。同时,光照与地形通过复杂的互作共同影响苔藓植物的谱系多样性