Simultaneous Determination of Ultra-trace Level of As,Sb,Bi,Ge,Sn and Hg in Coastal and Estuarine Seawater by Chemical Vapor Generation and Inductively Coupled Plasma Mass Spectrometry

建立了化学蒸汽发生(CVg)电感耦合等离子体质谱(ICP MS)同时测定近岸及河口海水中超痕量AS、Sb、bI、gE、Sn和Hg元素的方法。研究了CVg过程中kbH4、盐酸和硫脲的浓度以及样品流速、载气流速、海水样品盐度等对方法分析性能的影响。结果表明,在0.70%盐酸、0.10 g/l硫脲、11.0 g/l硼氢化钾、载气0.40 l/MIn条件下,6种待测元素的信噪比最高。优化实验条件下,AS、Sb、bI、gE、Sn和Hg的质量浓度在0.1~5.0μg/l内呈良好线性,相关系数不低于0.996 6;方法对0.1μg/l加标样品的相对标准偏差(rSd,n=5)为3.9%~8.9%,实际海水样品的加标回收率为89%~112%;方法的流程空白为0.012~0.036μg/l,定量下限(lOQS,10σ)在0.006 7~0.026μg/l之间,可满足近岸及河口海水样品中超痕量元素测定的需要。A chemical vapor generation(CVG) and inductively coupled plasma mass spectrometric(ICP MS) method was proposed for the simultaneous determination of ultra-trace level of As,Sb,Bi,Ge,Sn,and Hg in coastal and estuarine seawater.Effects of operating parameters,including concentrations of KBH4,HCl and thiourea,pump flow rate and carrier gas flow rate on analytical performance were studied.The results showed that good signal to noise(S/N) ratios for all the analysed elements were obtained when 0.70% HCl,0.10 g/L thiourea,11.0 g/L KBH4,and 0.40 L/min carrier gas were used in the CVG procedure.The relative standard deviations(RSDs,n=5) for 0.10 μg/L spiked sample were in the range of 3.9%-8.9%.The spiked recoveries for the seawater samples were between 89% and 112%.The method blanks were in the range of 0.012-0.036 μg/L.The limits of quantitation(LOQs,10σ) for the developed method were obtained between 0.006 7 μg/L and 0.026 μg/L.The method could be applied in the simultaneous determination of As,Sb,Bi,Ge,Sn and Hg in coastal and estuarine seawater.近海海洋环境科学国家重点实验室自主创新项目(MELRI0703

The association between IgG1,IgG2 subclasses toward human papillomavirus16-like particles in the serum and cervical intraepithelial neoplasia

背景与目的:免疫球蛋白g(IMMunOglObulIn g,Igg)是血清和细胞外液中含量最高的免疫球蛋白,其某个亚类的升高,可能与宫颈上皮内瘤变(CErVICAl InTrAEPITHElIAl nEOPlASIA,CIn)的发生、发展及转归有密切相关。然而对于CIn患者血清人乳头瘤病毒样颗粒(HuMAn PAPIllOMAVIruS VIruS-lIkE PArTIClES,HPV VlPS)Igg1及Igg2抗体变化目前尚不明确。本研究旨在探讨CIn患者血清中HPV16VlPS-Igg1、Igg2亚类抗体的变化及其与不同级别宫颈病变的关系。方法:采用ElISA法检测HPV感染患者HPV感染患者CIn患者及子宫平滑肌瘤或宫颈炎患者血清中HPV16VlPS-Igg和HPV16VlPS-Igg1、Igg2亚类抗体的表达水平。结果:患者血清中HPV16VlPS-Igg和Igg1抗体含量随宫颈病变级别增高而增加,差异有统计学意义(P1),分别为100.00%、87.50%和75.00%,而CInⅡ~Ⅲ组中仅为9.52%,与前3组相比,差异有统计学意义(P1),可能与HPV清除和宫颈病变逆转有关。Background and purpose:The maximum content of immunoglobulin G was found in serum and extracellular fluid.The increase of some immunoglobulin G subclasses may closely correlate with the occurrence,development and the outcome of cervical lesions.However,the changes of IgG subclasses toward human papillomavirus 16-like particles(HPV16VLPs) in the serum of patients are still under investigation.This paper aimed to study this issue and also analyze the relationship between the expression of IgG subclasses and different grades of cervical lesions.Methods:The expression of IgG subclasses in 32 human papillomavirus,30 cervical intraepithelial neoplasia(CINⅠ) ,43 CINⅡ-Ⅲ,24 hysteromyoma and chronic cervicitis were examined by ELISA.Results:The absorbance value of HPV16-IgG,IgG1 increased with the grade of CIN(P1) from control group was 100%,87.50% for HPV infection group,75% for CINⅠ group,compared with that from CINⅡ-Ⅲ patients(9.52%) (P1) in serum from low grade cervical lesions group and normal control group,might indicate the clearance of HPV infection and the regression of cervical lesions

颈动脉内中膜厚度及斑块分级在预测冠心病中的作用

【目的】界定颈动脉内中膜厚度及其斑块分级在估测冠心病中的最佳预测值。【方法】用高频超声检测125例患者颈总动脉内中膜厚度、颈动脉分叉处内中膜厚度，以及颈动脉斑块分级，评价它们估测冠心病的作用，并用ROC曲线评价它们的诊断价值。【结果】冠心病组颈总动脉及分叉处内中膜增厚、斑块分级高，与对照组比较差异有统计学意义，P〈0．05；ROC分析显示颈总动脉及分叉处内中膜增厚、斑块分级均可估测冠心病，其曲线下面积分别为0．70、0．75、0．74；将颈总动脉分叉处内中膜增厚≥1．3mm，颈总动脉内中膜增厚≥0．9mm，颈动脉斑块分级≥2定义为阳性，3者联合评价其阳性积分，则ROC曲线下面积为0．82，以3者中任2项阳性为预测值估测冠心病，灵敏度95．3％，特异度84．2％，阳性预测值85．3％，阴性预测值86．4％。【结论】颈动脉超声检查颈总动脉及其分叉处内中膜厚度、颈动脉斑块分级可用于估测冠心病。以3者联合评价诊断价值高

健康小鼠进食绣球菌对腹腔巨噬细胞分泌肿瘤坏死因子α的影响

测定健康小鼠进食绣球菌对腹腔巨噬细胞分泌肿瘤坏死因子α(TNF-α)的影响.SPF级Balb/C健康小鼠分组进食普通饲料、分别含绣球菌饲料、干品、浸汁饮水或其添加物组合、分别含酵母β-葡聚糖饲料、浸汁饮水或其添加物组合;进食一定时间后,分离腹腔巨噬细胞,使用含质量分数10%胎牛血清的RPMI1640培养液培养,TNF-α试剂盒检测上清液中的TNF-α质量浓度.结果表明,强化进食绣球菌组合(绣球菌饲料+干品+浸汁)的小鼠与对照组或酵母β-葡聚糖组合(酵母β-葡聚糖饲料+浸汁)小鼠相比,体质量不升反降;分离的腹腔巨噬细胞培养6 d,酶联免疫吸附测定技术检测上清液TNF-α,其质量浓度远高于其它两组.研究提示,强化绣球菌进食组合能够提高健康小鼠免疫力,并可能有利于控制体质量.福建省自然科学基金资助项目(2013J01394);;吴阶平医学基金会临床科研项目(320.6750.15231);;空军军医大学国重开放课题(CBSKL201729);;福建省中医药大学校管课题(XB2015077

结核分枝杆菌相关γ-干扰素体外释放试验在结核病诊断中的应用价值

目的探讨结核分枝杆菌相关γ-干扰素体外释放试验(Tb-IgrA)在结核病诊断中的应用价值。方法用国产Tb-IgrA试剂定量检测268例结核病患者和104例体检健康者外周血结核特异性γ-干扰素的含量,同时与澳大利亚QfT-gIT试剂和结核菌素纯化蛋白质衍生物(PPd)皮试进行平行比较分析。结果国产Tb-IgrA试剂、QfT-gIT试剂和PPd皮试3种方法的敏感性分别为90.7%、88.1%和75.4%,特异性分别为76.9%、80.8%和54.8%,阳性预测值分别为91.0%、92.2%和81.1%,阴性预测值分别为76.2%、72.4%和46.3%,准确性分别为86.8%、86.0%和69.6%。三种方法对肺结核与肺外结核的检测效果无显著差异。结论 Tb-IgrA法对诊断结核病有较高的敏感性和准确性,在结核病防控中有较好的应用价值。国家十一五“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项资助项目(2008ZX10003-004

省域水上光伏发电潜力分析评估方法及应用

分布式光伏是土地资源紧张条件下推进“双碳”目标实现的重要途径。水上光伏作为近年来一种创新的分布式光伏开发方式受到了广泛关注，现行政策也对水上光伏开发做出了细致管理规定。提出一种基于遥感信息的水上光伏发电潜力评估方法，旨在为水上光伏发电资源与项目开发的科学论证和精准评估提供一种手段。方法基于开放的遥感影像处理平台获取遥感影像并计算水体指数，通过大津法确定阈值、提取全部水体面积，并利用制图工具ArcMap按照各项影响因素对水面面积逐级折减；然后通过将水体划分成若干格网，计算各格网内可安装光伏水体面积和可配置光伏装机容量；结合典型气象年太阳辐射数据得到水上光伏发电潜力；最后，计算不同开发率下水上光伏发电潜力。以江苏省为例进行了水上光伏发电潜力评估分析并进行了验证，提供了一种在省域范围进行水上光伏发电潜力评估的技术手段

RE-X二元合金相图的热力学数据库

利用CALPHAD方法,采用亚正规溶体模型、亚点阵模型以及理想气体模型来描述RE-X(Ag,Bi,Cr,Mn,Mo,V,Zn)中二元系各相的Gibbs自由能,并结合相平衡及热力学性质的实验结果,对Ag-RE(RE:Sc,Y,Nd,Sm,Gd,Tb,Ho,Er)、Bi-RE(RE:Nd,Tm,Er,Ho,Pr,Gd)、Cr-RE(RE:Ce,Nd,Sm,Lu)、Mn-RE(RE:Pr,Nd,Sm,Eu,Tb,Dy,Ho,Er,Tm,Yb,Lu)、Mo-RE(RE:Sc,Y,La,Ce,Pr,Nd,Sm,Eu,Tb,Dy,Ho,Er,Tm,Yb,Lu)、V-RE(RE:La,Ce,Pr,Nd,Ho,Lu)和Zn-RE(RE:Y,Ce,Pr,Nd,Sm)各二元系相图进行热力学优化与计算。计算结果与实验数据取得很好的一致性,并结合其他相关稀土二元系相图热力学计算,初步建立部分稀土二元合金相图的热力学数据库。该热力学数据库可以提供相平衡及热力学性质等多种信息,为外推计算稀土多组元体系的相平衡提供理论基础,并为高性能稀土合金材料的设计及制备提供重要的理论指导

Preparation and biological safety of basic fibroblast growth factor/double-layered collagen composite

背景:胶原特殊的分子结构和生物活性有利于多种细胞黏附、增殖和分化,并可降解为新生组织提供足够空间。目的:制备一种复合负载碱性成纤维细胞生长因子的壳聚糖-肝素纳米粒子双层胶原基复合材料,并评价其生物安全性。方法:制备交联风干胶原膜和交联冻干胶原膜。将壳聚糖-肝素纳米粒滴于交联冻干胶原膜上,再将湿态交联风干胶原膜置于复合纳米粒子的交联冻干胶原膜上风干,即碱性成纤维细胞生长因子/双层胶原基复合材料。采用急性全身毒性试验、溶血试验、热原试验和细胞毒性试验评价其生物安全性。结果与结论:碱性成纤维细胞生长因子/双层胶原基复合材料为双层结构,一侧表面致密,另一侧疏松多孔。在其中间负载碱性成纤维细胞生长因子的壳聚糖-肝素纳米粒子呈不规则球形分布于胶原膜内侧面;急性全身毒性试验、热原试验、溶血试验均为阴性,细胞毒性为0级。说明碱性成纤维细胞生长因子/双层胶原基复合材料具有良好的生物安全性,对机体无毒,符合ISO10993-1评价标准。BACKGROUND: Collagen is a common used scaffold in tissue engineering, its specific molecular structure and biological activity are conducive to a variety of cell adhesion, proliferation and differentiation, and can speed up wound healing and degradation to provide sufficient space for new tissues.OBJECTIVE: To prepare a kind of inhomogeneous double layered collagen composite incorporated with basic fibroblast growth factor (bFGF) loaded chitosan-heparin (CS-Hep) nanoparticles, and to evaluate its biological safety.METHODS: The air-dried dense layer collagen membranes and freeze-dried loose layer collagen membrane were prepared respectively and then cross-linked by D-ribose (termed as CAM and CFM respectively).CS-Hep nanoparticles were prepared and then dropped on the surface of CFM.The wet CAM was laid on the surface of CFM with nanoparticles, and then air dried completely at 4 ℃.The obtained material was termed as bDM.Its physical and chemical properties were investigated.In addition, its bio-safety was also examined through acute systemic toxicity, pyrogen, hemolysis and cellular toxicity tests.RESULTS AND CONCLUSION: The bFGF/double-layered collagen composite was made up of double layers with one dense layer (CAM) and another loose layer (CFM).As part of bDM, CS-Hep nanopartiles with irregular global shape were aligned in the scope of D-period structure of the inner surface of CFM; the bFGF/double-layered collagen composite had no systemic acute toxicity, no cytotoxicity and pyrogen reactions as well as no hemolytic effect.The bFGF/double-layered collagen composite is prepared and found to have good biocompatibility and safety.Moreover, this material conforms to the ISO 10993-1, and can be used as a basic scaffold material in tissue engineering天津市科技支撑计划重点项目(08ZCKFSF02100);博士点基金项目(20101106110042)---

第十八届美国理论与应用力学大会总结

1会议概况2018年6月5—9日,第18届美国理论与应用力学大会(18th U.S. National Congress of Theoretical and Applied Mechanics, USNCTAM2018)在美国芝加哥召开.本次大会由美国力学国家委员会和中国力学学会联合主办,旨在探讨和交流近四年世界范围内在理论和应用力学领域的基础研究、创新技术的最新进展,吸引了来自世界各地的近千名专家学