Development of a quantitative ELISA detection method for Coxsackievirus A group 16 strain(CA16) antigen

目的:建立柯萨奇病毒A组16型(CA16)抗原的双抗体夹心ElISA定量检测方法,用于CA16灭活疫苗的研发和生产过程的抗原定量检测。方法:以CA16中和单抗T26H12为包被抗体、nA14b9为标酶抗体,构建定量检测CA16抗原的双抗体夹心ElISA方法,并对方法的特异性、灵敏度、精密度、准确性、线性和稳定性进行分析。结果:建立了双抗体夹心定量检测CA16抗原的ElISA方法。方法的线性相关系数r2=0.998,线性范围为8~128 ng/Ml,定量限度为8 ng/Ml;变异系数CV80%;与CA16以外的其他样本没有交叉反应。结论:构建的CA16抗原ElISA定量检测方法的各项性能符合定量检测需要,可用于CA16疫苗的研发和生产过程的抗原活性的定量检测。Objective:To develop an a quantitative enzyme linked immunosorbent assay(Q-ELISA) to determine the concentration of Coxsackievirus A Group 16 Strain(CA16) antigen.This method was used to determine CA16 antigen content at each stage of CA16 vaccine developing and manufacturing process.Methods:A double antibody sandwich Q-ELISA was developed to determine concentration of CA16 antigen,which was based on the high-affinity neutralizing monoclonal antibodies T26H12 as capture antibodies,and NA14B9 as HRP-labeled antibody.The performance of reagent were evaluated.Results:The Q-ELISA for CA16 antigen content was successfully developed.The reagent had good performance.The quantitation scope was 8-128 ng/ml,the coefficient correlation was 0.998,the limit of detection was 8 ng/ml,the recovery was between 87% and 113.8%.The stability was up to 80% after reagent was heated for 6 days at 37℃.The variation coefficient was lower than 15%,and thereagent was no reaction with other sample except CA16 antigen.Conclusion:The Q-ELISA for CA16 antigen was developed with good specificity,accuracy,precision and stability.The method can be used to determine CA16 antigen content during development and production of CA16 vaccine

废弃地向绿地的转变--封场垃圾填埋场生态更新研究[C]

封场垃圾填埋场被视为城市区域典型的废弃地。随着发展中国家固体废弃物的日益增加，封场垃圾填埋场得到越来越多重视并引发颇多隐患。本文以封场垃圾填埋场为例，对欧洲和美国进行的废弃地更新为绿地的案例进行研究。在此基础上，为中国废弃地生态更新措施提出了建议如下：对于陈旧的封闭垃圾填埋场而言，场地更新的最为可靠的方式为把填埋垃圾彻底清除、以更为可靠的方式运送到其他场地重新处置，再对场地修复。如果条件所限，上述工程目前无法开展，则可对封场垃圾填埋场区域作为绿地用途建设，加以防护并进行生态更新，从而尽可能消除其不良影响。对于发展中国家而言，固体废弃物的减量化、稳定化和再利用应当是垃圾相关问题的根本的应对之道

Prediction of Energy Resolution in the JUNO Experiment

International audienceThis paper presents the energy resolution study in the JUNO experiment, incorporating the latest knowledge acquired during the detector construction phase. The determination of neutrino mass ordering in JUNO requires an exceptional energy resolution better than 3% at 1 MeV. To achieve this ambitious goal, significant efforts have been undertaken in the design and production of the key components of the JUNO detector. Various factors affecting the detection of inverse beta decay signals have an impact on the energy resolution, extending beyond the statistical fluctuations of the detected number of photons, such as the properties of liquid scintillator, performance of photomultiplier tubes, and the energy reconstruction algorithm. To account for these effects, a full JUNO simulation and reconstruction approach is employed. This enables the modeling of all relevant effects and the evaluation of associated inputs to accurately estimate the energy resolution. The study reveals an energy resolution of 2.95% at 1 MeV. Furthermore, the study assesses the contribution of major effects to the overall energy resolution budget. This analysis serves as a reference for interpreting future measurements of energy resolution during JUNO data taking. Moreover, it provides a guideline in comprehending the energy resolution characteristics of liquid scintillator-based detectors