电针“梁门”-“足三里”对急性胃黏膜损伤模型大鼠血清代谢物动态表达的影响

目的采用核磁共振氢谱技术(1H NMR)探讨电针梁门-足三里治疗急性胃黏膜损伤的可能作用机制。方法 54只SD大鼠随机分为正常组、模型组、电针组,各组又分为干预1、4、7天3个时间点,每个时间点6只。除正常组外其余各组采用75%乙醇灌胃法复制急性胃黏膜损伤大鼠模型。造模成功后电针组大鼠用鼠板捆绑固定,采用疏密波(4 Hz,50 Hz),电针"梁门"(负极)"足三里"(正极),每次30 min,每日1次。正常组和模型组大鼠用鼠板捆绑固定30 min,每日1次。分别于干预1、4、7天后光镜观察胃黏膜病理形态,检测大鼠血清1H NMR谱,并利用模式识别方法分析不同干预时间下代谢物谱的差异,用正交偏最小二乘判别分析(OPLS-DA)模型和独立样本t检验,以P <0. 05筛选潜在的生物代谢标志物。结果病理结果显示,正常组大鼠胃黏膜表面细胞完整,未见溃疡性损害。干预1、4天时模型组大鼠可见胃黏膜上皮结构破坏严重,毛细血管增生及出血;干预4天时电针组大鼠胃黏膜的黏膜结构恢复较为完整,较模型组明显改善。血清代谢组学结果显示,正常组与模型组分离出差异代谢物6个:甘油磷酸胆碱、谷氨酰胺、甜菜碱、葡萄糖、乙酸盐和胆碱。与模型组比较,干预1天时电针组胆碱、甘油磷酸胆碱、葡萄糖、乙酸盐、谷氨酰胺和甜菜碱均升高;干预4天时胆碱、葡萄糖、谷氨酰胺和甜菜碱升高,甘油磷酸胆碱和乙酸盐降低;干预7天时甘油磷酸胆碱降低,葡萄糖、乙酸盐、谷氨酰胺和甜菜碱升高,胆碱无明显变化。结论电针"梁门"-"足三里"可有效回调急性胃黏膜损伤大鼠血清相关差异代谢物,恢复相应代谢通路稳定,以电针4天效果最佳,可能是其逆转胃黏膜损伤的作用机制之一。国家自然科学基金(81473751);;深圳市科技计划(JCYJ20160406140612883

艾灸干预胃溃疡大鼠胃黏膜脑肠肽的动态表达

目的:观察艾灸干预胃溃疡大鼠胃黏膜相关脑肠肽的动态表达,探讨艾灸干预胃溃疡的时效关系。方法:SD大鼠72只随机分为3批,各批分为正常组、模型组、胃经穴位组和非经穴位组(n=6)。采用70%乙醇灌胃复制胃溃疡大鼠模型。胃(非)经穴位组的大鼠进行艾灸1、4、7d(30min/d)。干预结束后于光镜下观察胃黏膜形态,RT-PCR测定胃黏膜增殖细胞核抗原(PCNA)及P物质(SP)表达。结果:与正常组比较,模型组大鼠胃黏膜溃疡灶明显,胃黏膜PCNA表达降低(P<0.05),SP表达升高(P<0.05);与模型组比较,干预1d后胃经穴位组大鼠胃黏膜PCNA表达升高(P<0.05),干预4d后胃经穴位组PCNA表达升高(P<0.05),SP降低(P<0.05);干预7d后各组大鼠胃黏膜PCNA及SP表达无显著差异。结论:艾灸胃经穴位能有效调节相关脑肠肽指标,促进胃黏膜损伤修复,并具有一定的时效关系。深圳创新科技计划项目(No.JCYJ20160406140612883);;国家自然科学基金项目(No.81473751);;国家重点基础研究发展计划(973计划)(No.2015CB554502)~

Concentration Protile of Etchant Measured by Microelectrode Technique in the Process of Chemical Micromachining

利用微圆盘电极技术,测定了KBr、L-胱氨酸和硫酸组成的刻蚀溶液体系中Pt电极表面电化学氧化产生的刻蚀剂Br2浓度分布,为约束刻蚀剂层技术(CELT)中刻蚀体系的选择和优化提供更直观的依据.GaAs表面CELT微加工实验证明了用微圆盘电极测得的表面刻蚀剂的浓度分布趋势与微加工实验所得到的结果一致.A carbon-disk microelectrode was used to investigate the surface concentration profile of etchant Br2, which was electrogenerated on the Pt working electrode. The steady state reducing currents of Br2 at different distances away from the Pt electode was measured. The concentration profile was estimated from the current-distance variation curves as a function of different sampling times. Experimentally determined concentration profiles are in good agreement with those estimated from the microetching results. The microelectrode technique has offered a good method to choose suitable etching solution for chemical micromachining.国家高新技术发展规划(863项目)(2002AA404170);; 福建省自然科学基金计划资助项目(E0520001

Concentration protile of etchant measured by microelectrode technique in the process of chemical micromachining

A carbon-disk microelectrode was used to investigate the surface concentration profile of etchant Br-2, which was electrogenerated on the Pt working electrode. The steady state reducing currents of Br-2 at different distances away from the Pt electode was measured. The concentration profile was estimated from the current-distance variation curves as a function of different sampling times. Experimentally determined concentration profiles are in good agreement with those estimated from the microetching results. The microelectrode technique has offered a good method to choose suitable etching solution for chemical micromachining

离子液体的性质及其在催化反应中的应用

在对离子液体的国内外研究现状综合分析的基础上，对离子液体的结构—性能关系和性质变化规律进行了探讨，系统地介绍了离子液体作为溶剂或催化剂在催化反应中的应用，特别是在金属催化、生物催化、反应—分离耦合方面的进展．在含微量水或无水离子液体中酶能够保持高的活性和选择性，有望在生物催化方面带来突破性进展．超临界CO2／离子液体及离子液体／水/有机相提供了一种新的反应—分离耦合模式，将进一步推动绿色化学的发展

离子液体的性质及其在催化反应中的应用

在对离子液体的国内外研究现状综合分析的基础上，对离子液体的结构—性能关系和性质变化规律进行了探讨，系统地介绍了离子液体作为溶剂或催化剂在催化反应中的应用，特别是在金属催化、生物催化、反应—分离耦合方面的进展．在含微量水或无水离子液体中酶能够保持高的活性和选择性，有望在生物催化方面带来突破性进展．超临界CO2／离子液体及离子液体／水/有机相提供了一种新的反应—分离耦合模式，将进一步推动绿色化学的发展

赴美考察湿地保护与环境教育见闻

赴美考察湿地保护与环境教育见闻陈克林，张从密，杨世和，赵连生根据林业部与美国内政部签署的中美自然保护协议书“关于湿地／水禽管理政策和环境教育项目”要求，由林业部野生动物和森林植物保护司、林业部音像中心、吉林省林业厅、山东黄河三角洲国家级自然保护区有关..

沸石载体结构对甲烷无氧芳构化性能的影响

考察了担载ＭｏＯ３的沸石催化剂上甲烷的无氧芳构化性能，并与沸石结构相关联。结果表明，孔径与苯分子动态直径相当的ＺＳＭ－５、ＺＳＭ－８、ＺＳＭ－１１和β沸石等是甲烷无氧芳构化催化剂的良好载体，其中３％ＭｏＯ３／ＨＺＳＭ－１１具有最高的甲烷芳构化活性和稳定性，９７３Ｋ下的转化ＨＭＣＭ－４１和ＨＳＡＰＯ－３４为载体时芳构化活化很低，以ＨＭＯＲ、ＨＸ和ＨＹ为载体时仅有少量乙烯生成，而以ＨＳＡＰＯ－５和Ｈ

Preparation and Supercapacitor Properties of Yttrium Doped NiO

应用化学共沉淀法制备掺Y的纳米N iO.XRD分析表明,Y的添加不改变N iO的晶体结构,但晶粒尺寸有所减小.利用恒流充放电、循环伏安和交流阻抗等测试方法对Y-N iO电极材料在5 mo.lL-1KOH溶液中作电化学性能测试.结果表明,化学掺杂提高了电极的比容量,当N iO与Y2O3的质量比为1∶0.1时,单电极放电比容量可达到363 F.g-1,比纯氧化镍电极提高了2.02倍.交流阻抗测试显示,Y掺杂降低了电解液在电极中的扩散阻力,提高了质子扩散能力,从而表现了更好的电容性能.Yttrium doped Nano-NiO were synthesized by chemical precipitation method.Characterization and performance of the samples were studied by X-ray diffraction,the results showed that the doping Yttrium did not change the crystal structure of NiO,but the size of crystalline grain was reduced.The electrochemical properties of the electrode materials were measured in the three electrode system.Electrochemical properties of NiO were researched by cyclic voltammetry,constant current charge/discharge and AC impedance methods.It could be seen that the chemical doping improved the specific capacitance and the utilization of active materials.When the weight ratio of NiO with Y_(2)O_(3)was 1∶0.1,the composite material had the best properties.The maximum discharge specific capacitance was 363F g~(-1)in the 5 mol·L~(-1) KOH solution.The result of AC impedance showed that the doping could improve the electrochemical reversibility,reduce the resistance of cations transfered between the electrode and electrolyte which made the electrodes show the better capacitive behavior.作者联系地址：哈尔滨工程大学化工学院,哈尔滨工程大学化工学院,哈尔滨工程大学化工学院,哈尔滨工程大学化工学院,哈尔滨工程大学化工学院,吉林化工学院 黑龙江哈尔滨150001,黑龙江哈尔滨150001,黑龙江哈尔滨150001,黑龙江哈尔滨150001,黑龙江哈尔滨150001,吉林吉林132022Author's Address: 1,CHEN Ye~*1,ZHANG Mi-lin~1,SHU Chang~1,ZHANG Chun-xia~1,XU Peng-cheng~21.School of Chemical Engineering,Harbin Engineering University,Harbin 150001,Heilongjiang,China,2.Jilin Institute of Chemical Technology,Jilin 132022,Jilin,Chin