Review of sea anemones compound and bioactivity

海葵(sea anemone)又名海菊花,多指腔肠动物门(Coelenterata)珊瑚虫纲(Anthozoa)海葵目(actiniaria)动物。海葵一般分为爱氏海葵科、链索海葵科、细指海葵科、投海葵科、固边海葵科和绿海葵科等6科37种[1]。海葵体型大多呈圆筒状,触手以辐射对称状在口周围形成数轮,广泛分布在热带和温热带海域,主要固着在海中岩石上或泥沙中。我国海域均有分布

Progress on Fucoxanthin and Fucoxanthin-chlorophyll Protein （ FCP） Complexes

岩藻黄素又称岩藻黄质,是广泛存在于海洋藻类中的一种光合色素,为类胡萝卜素的含氧衍生物。岩藻黄素-叶绿素蛋白复合体(FCP)是由岩藻黄素和叶绿素共同组成的高效捕光蛋白复合物。主要综述了岩藻黄素的理化性质、生物活性、和提取方法以及FCP的功能、结构和提取方法

PURIFICATION of P49 ANTIGEN GENE PRODUCT of TRICHINELLA SPIRALLS EXPRESSED IN ESCHERICHIA COLI

目的建立旋毛虫P49抗原基因原校表达产物的纯化方法。方法菌体经冻融、超声破菌及提取包涵体后，用离子交换和凝胶过滤层析纯化蛋白质。结果纯化后的融合蛋白P49/gST经SdS-PAgE电冰鉴定达电泳纯。双抗体夹心ElISA法检测结果表明纯化的P49/gST融合蛋白能与旋毛虫感染的鼠血清和纯化融合蛋白免疫的兔血清反应，而不与正常民和兔血清反应。结论纯化后的融合蛋白P49/gST具有较高的纯度及较强的免疫活性，可望作为旅毛虫病的免疫诊断抗原。Aim To establish a purification procedure for p49 antigen gene product of Trichinella spiralis expressed inEschedchia coli.Methods After centrifugation of the bacterial culture, the bacterial pellet was resuspended and lysed byfreezing-thawing treatment and ultrasonication.Ion exchanged and gel filtration chromatography were used to purify the fu-sion protein p49/GST from the inclusion bodies- Re8ultS The purity of the fusion protein p49/GST was verified using SDS-PAGE.The purified p49/GST protein could react with mouse antisera against Tricinella sPiralis and rabbit antisera againstpurified p49/GST protein, but not with normal mouse and rabbit sera by sandwich EL1SA.Concluslon The purified fusionprotein p49/GST with high purity and good immune activity could be used for the immune aasay of trichinellosis.福建省重点（农医）项目!95－Z－15

基于带权词格的循环神经网络句子语义表示建模

目前,循环神经网络(recurrent neural network, RNN)已经被广泛应用于自然语言处理的文本序列语义表示建模.对于没有词语分隔符的语言,例如中文,该网络以经过分词预处理的词序列作为标准输入.然而,非最优的分词粒度和分词错误会对句子语义表示建模产生负面作用,影响后续自然语言处理任务的进行.针对这些问题,提出基于带权词格的循环神经网络模型.该模型以带权词格作为输入,在每个时刻融合多个输入向量和对应的隐状态,融合生成新的隐状态.带权词格是一种包含指数级别分词结果的压缩数据结构,词格中的边权重在一定程度上体现了不同分词结果的一致性.特别地,利用词格权重作为融合函数中权重建模的监督信息,进一步提升了模型句子语义表示的学习效果.相比于传统循环神经网络,该模型不仅能够缓解分词错误对句子语义建模产生的负面影响,同时使得语义建模具有更强的灵活性.在情感分类和问句分类2个任务上的实验结果证明了该模型的有效性.国家自然科学基金项目(61672440);;北京语言大学语言资源高精尖创新中心资助;;国家语言文字工作委员会一般项目(YB135-49);;中央高校基本科研业务费专项资金项目(ZK1024);;苏州大学江苏省计算机信息处理技术重点实验室开放课题(KJS1520)~

Diatom diet selectivity by early post-larval abalone Haliotis diversicolor supertexta under hatchery conditions

Benthic diatoms constitute the primary diet of abalone during their early stages of development.To evaluate the dietary preferences of early post-larval abalone,Haliotis diversicolor supertexta,we analyzed the gut contents of post-larvae that settled on diatom films.We compared the abundance and species diversity of diatom assemblages in the gut to those of the epiphytic diatom assemblages on the attachment films,and identified 40 benthic diatom species in the gut contents of post-larvae 12 to 24 d after settlement.The most abundant taxa in the gut contents were Navicula spp.,Amphora copulate,and Amphora coffeaeformis.Navicula spp.accounted for 64.0% of the cell density.In the attachment films,we identified 110 diatom species belonging to 38 genera.Pennate diatoms were the dominant members including the species Amphiprora alata,Cocconeis placentula var.euglypta,Cylindrotheca closterium,Navicula sp.2,and A.coffeaeformis.Nano-diatoms(<20 μm in length) accounted for a considerable proportion of the total species..

In vitro study of cholesterol succinyl chitosan anchored liposomes as a carrier for epirubicin

背景:多糖"锚定"脂质体在抗肿瘤药物、蛋白以及基因的传输领域有着极其重要的理论及应用价值,国外已有较多机构在进行相关的研究。目的:通过"锚定"的方式制备胆甾醇琥珀酰基壳聚糖锚定脂质体,并以表阿霉素作为模型药物,考察其对包载药物体外释放性质的影响。设计、时间及地点:体外实验,于2006-09/2008-05在天津市生物医学材料重点实验室完成。材料:以壳聚糖为原料,合成胆甾醇琥珀酰基壳聚糖,并采用胶体滴定法测定其胆甾醇基取代度。方法:采用pH梯度法制备表阿霉素脂质体,然后通过共孵育的方法合成了取代度为2.80%,5.58%和8.00%的载药胆甾醇琥珀酰基壳聚糖锚定脂质体。主要观察指标:荧光分光光度计检测药物浓度;透射电镜观察脂质体形态;亚微米粒度及电位分析仪检测脂质体的粒径大小、分布和电位;动态透析法考察包载药物表阿霉素在胆甾醇琥珀酰基壳聚糖锚定脂质体中的体外释放特征。结果:胆甾醇琥珀酰基壳聚糖锚定脂质体为规则球状形态,呈现典型的核壳结构,粒径为245.4~279.7nm,zeta电位为+21.09~+25.48mV;和载药脂质体及壳聚糖包衣脂质体相比,CHCS锚定脂质体能明显延缓表阿霉素的体外释放,在胆甾醇基取... 【英文摘要】 BACKGROUND: Polysaccharides anchored liposomes play an extremely important role in the fields of antitumor drug, protein and gene transmission. Related research is also present abroad. OBJECTIVE: To prepare cholesterol succinyl chitosan (CHCS) anchored liposomes, and to investigate the effect of CHCS anchored liposomes on the release property in vitro of loading drugs taking epirubicin as a model drug. DESIGN, TIME AND SETTING: A study in vitro was performed in the Key Laboratory of Biomedical Materials o

A TWO-PARTICLE TURBIDIMETRIC LATEX IMMUNOASSAY FOR THE DETECTION OF SPECIFIC ANTIBODIES OF TRICHINELLA SPIRALIS

目的　建立旋毛虫病的快速检测技术。方法　基因工程抗原包被有色乳胶颗粒，抗抗体包被磁性颗粒，在抗体存在下形成抗原－ 乳胶－ 抗体－ 抗抗体－ 磁性颗粒的复合场，在磁场作用下沉淀下来，从而达到快速检测旋毛虫相关抗体的目的。结果　１９ 份人工感染鼠血清、５ 份人工感染猪血清、３ 份旋毛虫病猪血清、４ 份病人血清均呈阳性反应，而对照血清均为阴性反应；该方法在鼠感染旋毛虫后第五天可测出ＩｇＭ 抗体，第九天可测出ＩｇＧ抗体。结论　本检测技术简便易行，不需专用设备，可望成为一种快速诊断旋毛虫病的有效方法。Aim To establish a rapidly detecting method for the specific antibodies of Trichinella spiralis Method Latex was coated with p49/GST and polystyrene beads(Dynabeads)were coated with anti-antibodies SAM-IgG、SAH-IgG、RAP-IgG or SAM-IgM Then the test serum was incubated with two particles for 30 min at room temperature with slowly shaking After sedimentation of the polystyrene beads with a magnet,the turbidity of the resultant latex suspension was estimated with eyes or measured spectrophotometrically at a wavelength of 400nm Results Positive result of IgG antibody were found in 19 sera of experimental infected mice,3 sera of experimental infected swine and 4 sera of patients The IgM and IgG can be check up in mice after 5 and 9 days after experimental infection The result agreed with those obtained by ELISA Conclusion A two-particle turbidimetric latex immunoassy established by this study is a rapid,easy and precise method for the diagnosis of the trichinosis福建省科委(农医)项目!(95-Z150

DIAGNOSIS OF TRICHINOSIS BY ELISA WITH P49/GST ANTIGEN

目的以纯化的融合蛋白 p49/GST为抗原建立ELISA检测方法。方法对一批试验血清进行间接ELISA检测。结果 19份人工感染鼠血清、5份人工感染猪血清、4份旋毛虫病猪血清、4份病人血清呈IgG抗体阳性 ,2 1份人工感染鼠血清呈IgM抗体阳性 ,而正常对照血清及 30 0份屠宰场待检猪血清均呈阴性反应 ,其结果与常规压片法结果相符。结论融合蛋白p49/GST对于研制旋毛虫病的诊断抗原具有的潜在应用价值Aim To establish ELISA detecting method for the specific antibodies of Trichinella spiralis.Method A series of confirmed trichinosis sera were detected with ELISA with fusion protein p49/GST.Results Positive results of IgG antibody were found in 19sera of experimental infected mouse and 3sera of infected swine and 4 sera of patients;positive results of IgM antibody were found in 21 sera of experimental infected mouse .All normal sera were negative. Conclusion The ELISA with p49/GST can be regarded as a sensitive, specific immunological method for the diagnosis of trichinosis.福建省重点(农医 )项目资助!(项目号 :95 -Z -15 0

弹道靶上的跨音速实验

本文介绍用弹道靶作跨音速试验的新方法,在二级轻气炮上采用了二个特殊的新技术:充气放炮能发射模型达到亚音速,低充填条件能发射模型达到跨、超音速。由于初始加速度较小,发射的模型外形完整,姿态稳定;加上没有支撑干扰和小洞壁效应的优点,使弹道靶设备在跨音速实验方面有特殊的优越性,用上述技术得到了圆球跨音速流场的清晰照片,其中击波脱体距离、分离点位置、颈部宽度、尾迹形状与超音速、高超音速流动比较有明显变化。文中M≈1.010和M≈0.99的二张全流场照片是十分有意义的。最后提出一个想法,用靶场加压、降温的方法可以提高雷诺数直到10~7以上

类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家