The Inhibition Effects of A Monoclonal Antibody Targeting Neuropilin-1 on Adhesion of Glioma Cell U251 to Fibronectin and The Associated Mechanism

摘要 研究背景和目的 脑胶质瘤是颅内最常见的原发性肿瘤，大多数为浸润性和膨胀性生长，预后相对较差。据美国统计的资料显示，在所有胶质细胞瘤中约占半数的胶质母细胞瘤患者1年生存率约30%，5年生存率不到5%。尽管目前采取最大限度的手术切除及术后放疗与化疗结合等综合治疗，但是恶性胶质瘤(malignantgliomas，MG)的临床预后仍不乐观。目前分子靶向治疗已经成为近年来肿瘤治疗的热点，但是靶向治疗肿瘤的效果仍然十分有限，因此，发现新的肿瘤治疗靶点显得极为重要。人神经菌毛素1（Neuropilin-1，NRP-1)是一种新型的多功能受体，近年来，大量研究结果显示NRP-1可表达于新生血管的内...Abstract Background and Purpose: Glioma is the most common primary intracranial tumors, most of which were growing expansively and invasively, and the prognosis is relatively poor. U.S. data show that among half of all gliom patients with glioblastoma 1-year survival rate is about 30%, 5-year survival rate of less than 5%. Despite maximum surgical resection and postoperative radiotherapy an...学位：医学硕士院系专业：医学院_外科学学号：2452010115331

团簇离子Fe_nP_m~+结构的量子化学研究

对激光等离子体反应生成的原子团簇 Ｆｅｎ Ｐｍ ＋ （ｎ＝ １～３， ｍ ＝ ２～１２）进行了量子化学从头算研究， 对可能的几何结构进行了试探性计算， 并对其中较稳定构型进行结构优化． 结果表明： Ｆｅ Ｐｍ ＋ 中， Ｆｅ 倾向与 Ｐ形成多配位磷化物． 其中 Ｆｅ Ｐ６＋ 较稳定． 同时， 铁与磷易形成多铁磷化物．讨论了 Ｆｅ２ Ｐ５＋ ～ Ｆｅ２ Ｐ８＋ ， Ｆｅ３ Ｐ２＋ ～ Ｆｅ３ Ｐ１２＋ 不同构型对其稳定性的影响

Effects of the Xitongxiao prescription on apoptosis and proliferation of chondrocytes in knee

目的：探讨膝痛消方对促进软骨细胞增殖和抑制IL-1β诱导的软骨细胞凋亡的作用。方法：体外培养膝关节软骨细胞，用阿利新蓝染色方法检测软骨细胞蛋白多糖，用Ⅱ型胶原免疫荧光染色检测软骨细胞Ⅱ型胶原的表达，以鉴定软骨细胞；用MTS检测软骨细胞的增殖；用IL-1β诱导软骨细胞凋亡，AnnexinV/PI双染检测细胞凋亡。结果：膝关节剥离得到的细胞形态为多角形或梭形，阿利新蓝与Ⅱ型胶原免疫荧光染色均呈阳性，证明分离培养的细胞为软骨细胞。MTS实验结果发现膝痛消方提取物处理软骨细胞后检测到的OD值随浓度增加而增加且显著高于对照组。此外，IL-1β处理诱导软骨细胞凋亡，而膝痛消方提取物能够有效地抑制IL-1β引起的细胞凋亡。结论：膝痛消方能促进软骨细胞的增殖，抑制IL-1β诱导的软骨细胞的凋亡。以上发现为膝痛消方临床上治疗膝关节骨性关节炎提供药理作用依据。Objective:To investigate the effects of the Xitongxiao prescription on the cell proliferation and the inhibition of IL-1β-induced apoptosis of chondrocytes.Methods:To identify chondrocytes,the articular chondrocytes were cultured in vitro;staining with alcian blue was used to detect the protein polysaccharide,and staining with collagen immunofluorescence was used to examine the expression of type II collagen in chondrocytes.The proliferation of chondrocytes was determined by MTS.Apoptosis of chondrocytes was induced by IL-1β,and the effect of apoptosis was tested by Annexin V/PI double staining.Results:The morphology of the cells obtained from the knee joint was polygonal or fusiform.The tested results of Alcian blue and collagen immunofluorescence staining were positive.These together certified that the isolated cells were knee chondrocytes.MTS assay results showed that the detected OD values were dosedependently increased and they were significantly higher than those of the control group.In addition,the apoptosis of chondrocytes was induced by IL-1βtreatment.While the extract of the Xitongxiao prescription could effectively inhibit the IL-1β-induced apoptosis of chondrocytes.Conclusion:The Xitongxiao prescription could promote the proliferation of chondrocytes and inhibit the apoptosis of chondrocytes induced by IL-1β.The above findings may provide one of pharmacological mechanisms of the Xitongxiao for clinical treatment of knee osteoarthritis.厦门市科技计划项目(3502z20144031);福建省自然科学基金计划资助项目(2015J01065)

Effects of mixed-culture densities of Spartina alterniflora on eco-physiological characteristics of Kandelia candel seedling

按照不同密度将互花米草与秋茄进行混种,对比研究胁迫条件下秋茄幼苗生长、光合特性及其渗透调节物质变化规律。结果表明,低密度互花米草促进秋茄的茎长以及各部分生物量,高密度则起抑制作用。随着互花米草密度的增大,秋茄幼苗叶片光合速率、气孔导度、蒸腾速率、水分利用率和蛋白含量均下降;相反,胞间CO2浓度、可溶性糖、淀粉、脯氨酸含量却上升,这些变化有利于对抗互花米草带来的不利影响。 【英文摘要】 Kandelia candel seedlings were mix-cultured with Spartina alterniflora of different densities for 120 days,and their growth,photosynthetic characteristics as well as osmotic adjustment law were studied.The results showed that the stem height and biomass of K.candel seedlings were enhanced with low density S.alterniflora and inhabited with high density ones.With the density of S.alterniflora going up,Pn、Gs、Tr、WUE and protein content in K.candel seedlings decreased respectively.On the contrary,the content of ...国家自然科学基金资助项目(30670317,30271065);; 福建省自然科学基金资助项目(D0210001

Food source and feeding habit of Helice tientsinensis from the common reed vegetation in high marsh of Yellow River Delta,China

Investigating the composition of food sources with stable isotope method can provide direct evidence for the top-down control in the coastal wetland.In this study,we examined food source and feeding habit of Helice tientsinensis of common reed (Phragmites australis) vegetation in high marsh of Yellow River Delta.The results showed that the density of crab was (5.5 1.5) ind&middot;m~(-2),with the behavior of climbing P.australis to feed on the leaves at night.Under the same indoor experimental condition,H.tientsinensis showed feeding preference on fresh leaves of P.australis.The stable isotope food source analysis showed that the leaves of P.australis were one of the important food sources of H.tientsinensis in the field.There were temporal variations in the proportion of fresh leaves [May:(6.44.9) %,July:(5.84.9) %,September:(12.58.8) %] and dead leaves [May:(12.47.8) %,July:(15.59.9) %,September:(15.19.4) %].Therefore,H.tientsinensis could inhibit P.australis&#39;s growth and affect litter decomposition through feeding disturbance behavior.</p

用流式细胞仪研究秋茄叶柄薄壁细胞大小及叶绿素含量与海水盐度的关系

采用快速离析方法和流式细胞仪研究了红树植物秋茄成熟叶柄薄壁细胞的大小和叶绿素含量与海水盐度之间的关系。结果表明 ,海水盐度为 0 .8%～ 2 .0 %时 ,随着盐度的升高 ,叶柄薄壁细胞大小和单位体积叶绿素含量的变化趋势与叶肉细胞一致。海水盐度为 0 .8%～ 1.0 %时 ,随着盐度升高 ,向有利于提高光合作用强度的方向发展 ;海水盐度在 1.0 %～ 2 .0 %之间时 ,随着盐度的升高 ,向加强生理干旱适应性方向发展。海水盐度高于2 .0 %时 ,叶柄薄壁细胞的大小和单位体积薄壁组织叶绿素含量随盐度的变化趋势与叶肉细胞不同 ,原因尚待进一步研究。实验结果表明 ,借助快速离析方法 ,使用流式细胞仪研究器官细胞相关特性是可行的 ,为从形态学角度研究红树植物耐盐机理提供了可能 ,扩大了流式细胞仪的应用领

Advance in Red-emitting Mn⁴⁺-activated Oxyfluoride Phosphors

稳定可靠的高光子能量发光(620~650 nm)红光荧光粉, 对于构建低色温、高显指荧光粉转换型白光发光二极管(WLED)至关重要。Mn⁴⁺激活红光荧光粉是当前 WLED 用荧光粉研究热点之一。本文介绍了 Mn⁴⁺离子的能级跃迁与光致发光特性, 详细叙述了目前所报道的七种 Mn⁴⁺激活含 d⁰/d¹⁰/s⁰离子氧氟化物系列红色荧光粉(如Na₂WO₂F₄:Mn⁴⁺等)的制备方法、晶体结构及其发光特性。目前⁴⁺在氧氟化物结构中得到强 R 线(零声子线)发光的情况少, 微观配位体仍是[MnF₆]或[MnO₆], 其化学稳定性和量子效率研究也很缺乏。最后对 Mn⁴⁺激活氧氟化物红光荧光粉的研究进行了展望。The stable and reliable red phosphor with high-photon energy emission (620–650 nm) is critical for the fabrication of the phosphor-converted white light-emitting diode (WLED) with low correlated color temperature and high color rendering index. Mn⁴⁺-activated phosphor is an emerging kind of red-emitting phosphor for WLED. Herein, the energy levels transition and photoluminescence characteristics of the Mn⁴⁺ ion were introduced; then, the preparation, crystal structure and luminescent properties of as-far reported seven kinds of Mn⁴⁺-doped oxyfluoride red phosphors (such as Na₂WO₂F₄:Mn⁴⁺) containing d⁰/d¹⁰ or s⁰ cations were reviewed. Currently, only in quite rare case of oxyfluoride, Mn⁴⁺ was found to exhibit strong R-line emission, with local coordination remaining as either [MnF₆] or [MnO₆]. The studies on the chemical stability and quantum efficiency of Mn⁴⁺ -doped oxyfluoride phosphors are still insufficient. Finally, we prospected the future development of Mn⁴⁺-doped oxyfluoride phosphor

连续陶瓷纤维的制备、结构、性能和应用:研究现状及发展方向

连续陶瓷纤维是纤维增强陶瓷基复合材料的增强体,对提高陶瓷基复合材料的强度和韧性起关键作用,高损伤容限和高强度陶瓷纤维是阻止裂纹扩展实现陶瓷基复合材料强韧化的保障。本文对碳化硅、氮化硅、氮化硼、氧化铝和氧化锆等几种陶瓷纤维的制备方法、结构、性能和应用等方面进行了全面的综述,指出了今后的发展方向,期望为未来陶瓷纤维的研究、开发及应用提供参考。国家高技术研究发展计划(863)(2003AA305630);;国家自然科学基金(51472144);;山东省科技重大专项(2015ZDZX11003);;山东省青年学者未来计划(2016WLJH27

抗人NRP1单克隆抗体的活性鉴定及初步应用

目的鉴定抗人神经菌毛素1(neuropilin1,NRP1)单克隆抗体的生物活性并利用该抗体检测常见肿瘤细胞株NRP1蛋白的表达情况。方法腹水法制备4株抗NRP1单克隆抗体并用rProtein A亲和柱纯化抗体,间接ELISA检测4株抗体的滴度水平,Western blot分析4株抗体结合NRP1蛋白的特异性,流式细胞术分析4株抗体结合NRP1蛋白的亲和力,共聚焦免疫荧光实验进一步验证抗体结合NRP1蛋白的能力;细胞免疫组织化学染色检测14株肿瘤细胞表达NRP1蛋白的情况。结果 4株抗体均能特异结合NRP1蛋白,其中A6结合NRP1能力最强,能结合在表达NRP1蛋白的细胞膜表面。细胞免疫组织化学染色结果显示NRP1在HepG2、C6、HEK293、BEL-7402、MDA-MB-453呈高水平表达,在U87、MGC803、MCF7、MDA-MB-231中表达水平相对较弱,而在U251、BGC823、H6、HT-29的表达介于两者之间,在SMMC-7721细胞中未检测到表达。结论 4株抗NRP1单克隆抗体均能特异结合NRP1蛋白,多种肿瘤细胞株均不同程度表达NRP1蛋白,为进一步探讨NRP1蛋白与肿瘤发生发展的关系奠定了基础

Separation and purification of catalpol from Radix Rehmanniae

An ultrasonic extraction technology of preparing catalpol from Radix Rehmanniae was optimized by a uniform experimental design.The optimum conditions for ultrasonic extraction were thus established: 68% (vol) ethanol aqueous solution as extraction solvent,the volume(ml) to mass(g) ratio of extraction solvent to feed of 5∶1,the ultrasonic treatment time of 36 min.The crude catalpol obtained from the optimized ultrasonic extraction technology was further purified by selective adsorption with macroporous resin.The adsorption capacities and desorption yields of ten kinds of macroporous resins for catalpol were investigated.The results showed that H103 resin possessed the best performance.The properties of dynamic adsorption and desorption of H103 resin for catalpol were subsequently investigated on adsorption column.The optimum conditions for adsorption and desorption were determined.The concentration of catalpol in the loaded sample solution was 6.15 mg·ml-1,the flow rate was 1.0 ml·min-1,the volumetric percentage of ethanol in the eluting solvent was 75%,the elution flow rate was 0.5 ml·min-1 and the elution volume was 3.33 BV(bed volume).Under the above conditions,the purity of catalpol product eluted from the column packed with H103 resin reached 62.39% while that of the crude catalpol was only 10.01%