补肾健脾方影响高脂肪饮食骨质疏松模型大鼠脂代谢及瘦素的变化

背景:绝经也可导致体内瘦组织与脂肪成分的改变,现阶段发现骨质疏松在肥胖的老年人中的发生率要大于非肥胖者,由此推断瘦素可能是骨质疏松症发病机制中的一个重要调节因素。目的:探讨补肾健脾方对高脂肪饮食骨质疏松大鼠脂代谢及瘦素的影响。方法:将6月龄SPF级雌性SD大鼠220只随机分成模型组170只和假手术组50只。模型组行双侧卵巢结扎切除术,假手术组除未行卵巢结扎切除外,其余步骤同模型组。手术后12周,模型鉴定成功后,手术组随机分成正常饮食模型组、高脂饮食模型组、高脂饮食健骨颗粒组和高脂饮食辛伐他丁组各40只。各组于术后第13周开始给药,健骨颗粒组给予健骨颗粒2 g/(kg·d),生理盐水2 mL溶解后灌胃,辛伐他丁组给予辛伐他丁100μg/(kg·d)灌胃,假手术组和正常饮食模型组以2 mL生理盐水灌胃,1次/d。于用药2,6,12,24周后测体质量,取血清、腰椎和股骨近端备用。双能X射线测定右侧胫骨骨密度,ELISA法测定血清骨钙素、血清抗酒石酸酸性磷酸酶5b(TRACT-5b)、总胆固醇、三酰甘油、瘦素、瘦素受体的水平,q PCR法测定大鼠骨组织瘦素、瘦素受体m RNA表达。结果与结论:①高脂饮食健骨颗粒组大鼠的体质量高于同期正常饮食模型组和高脂饮食模型组,但低于假手术组和高脂饮食辛伐他汀组,同时表达的瘦素、瘦素受体、骨钙素水平均高于正常饮食模型组和高脂饮食模型组,TRACT-5b、胆固醇、三酰甘油的浓度均低于正常饮食模型组和高脂饮食模型组,各组比较差异均有显著性意义(P <0.05);②结果表明,补肾健脾方可以调整大鼠脂代谢紊乱、降低血液黏滞度、改善血管壁结构的同时还能通过上调大鼠血清和骨组织中瘦素、瘦素受体的表达,改善大鼠骨代谢水平,防治原发性骨质疏松症。福建省自然科学基金(2017J01332),项目负责人:林煜;;福州市卫生系统科技项目(2017-S-wq15),项目负责人:李平~

茵蒲灌肠液联合美沙拉秦肠溶片治疗轻中度溃疡性结肠炎的临床效果

目的观察茵蒲灌肠液联合美沙拉秦肠溶片治疗轻中度溃疡性结肠炎(UC)的临床效果。方法选取2016年1月—2017年12月轻中度UC患者60例,按治疗方法不同分为对照组和观察组,每组30例。对照组给予美沙拉秦肠溶片,观察组在对照组基础上给予茵蒲灌肠液保留灌肠。比较2组治疗后的临床症状、结肠镜下黏膜改变、不良反应情况以及总体疗效。结果治疗后,观察组的临床症状缓解率、结肠镜下黏膜修复有效率以及总体疗效有效率高于对照组(P0.05)。结论茵蒲灌肠液联合美沙拉秦肠溶片对轻中度UC的临床症状缓解、结肠镜下黏膜修复程度均具有较好的效果,且不增加不良反应的发生率。福建中医药大学校管课题(XB2016066

旋转恒定磁场治疗激素性股骨头坏死的效果与病理机制

【目的】探讨旋转恒定磁场(RPMF)治疗激素性股骨头坏死的疗效和病理机理。【方法】制备新西兰兔股骨头坏死动物模型,予旋转恒定磁场(铋铁硼永磁体以8Hz频率旋转、形成其上方20cm直径60cm的半球形磁场、表面强度在0.32～0.60T之间)处理4周和8周(A1、A2组),使用组织切片和X线片以及显微CT观察股骨头内部结构变化,同时监测其血液黏稠度、胆固醇、甘油三酯以及髋关节腔压力等的变化,和相应阳性假处理4周、8周组(B1组\B2组)、空白对照(C组)进行统计学分析和比较。【结果】经旋转恒定磁场(RPMF)处理后,动物模型的股骨头骨小梁形态结构显著改善,尤以显微CT表现明显,髋关节腔压力、血液流变学及血脂检测值改善具有显著统计学意义(PEta2A*B>Eta2B)。【结论】磁场可以针对股骨头坏死主要环节高脂血症、血液黏稠度增加、关节腔压力增高等发挥调节作用,有效治疗激素性股骨头坏死,尤其是早期病变;显微CT在诊断激素性股骨头坏死方面具有灵敏性和直观的优越性

Centralized Remote Monitoring System for Bred Fish in offshore Aquaculture Cage

为克服离岸深水网箱群养殖水域环境和鱼群安全日常监测不便的问题,提出了一种集中式深水网箱群鱼群状态远程监测系统。采用水声多波束探测技术、多路信号处理技术设计了远程探测节点,用于扫描各深水网箱内部空间;采用虚拟仪器技术设计了本地监控中心,便于用户集中控制远程探测节点、查看网箱探测结果;采用gPrS无线数据传输技术构建了远程、实时的指令和数据传送通道。监测系统样机在福建省泉州市围头深水网箱养殖现场进行测试,结果表明:系统设计合理,操作方便,性能稳定、可靠,能够准确及时地反馈养殖环境变化情况和网箱鱼群状态,减轻养殖工作量。In order to monitor the aquatic environment and fish state in offshore aquaculture cage farm daily,a centralized remote monitoring system for bred fish was developed.The system was composed of remote detecting node(RDN) and local monitor and control center(LMCC).RDN based on the techniques of multi-beam detection and multi-path signal process was placed in the center of a cage to scan the inner cage and measure the aquatic environment.LMCC based on LabVIEW was placed in the office to manage all the remote detecting nodes remotely and show fish state.The communication between LMCC and RDN depended on GPRS wireless data transmission technology.The system prototype was tested in the field of offshore aquaculture cage farm in Quanzhou,Fujian province.The results show that the system is reasonable design,simple operation,easy placement,and robust under different environmental conditions.It can real-time monitor the aquatic environment and fish activity remotely,provide users with a variety of information such as fish amount,fish growth in the offshore fish cage,and give an alarm in the time when net clothing damage or fish escape.国家自然科学基金资助项目(41176032);中国博士后科学基金第五十批面上资助项目(2011M501082

体外循环中低流量肺动脉灌注对肺功能的影响

【目的】探讨体外循环中低流量肺动脉灌注对肺功能影响的作用机理。【方法】体质量18-22kg的杂种犬10只，随机分为对照组（n=5）和肺灌注组（n=5）。常规建立体外循环。肺灌注组用氧合血30mL／（kg·min）灌注肺动脉。分别于转流前，停体外循环即刻，转流后1h，转流后2h采集数据，计算氧合指数、肺动态顺应性。检测肺静脉血丙二醛（MDA）、超氧化物歧化酶（SOD）、细胞间黏附分子1（ICAM-1）和P选择素。【结果】转流后肺灌注组犬的肺动态顺应性和氧合指数明显高于对照组（P〈0．05）。两组肺静脉血MDA和SOD总体差异有统计学意义（P〈0．05），肺灌注组MDA含量低于对照组，SOD活性高于对照组。肺灌注组肺静脉血ICAM-1、P选择素含量均低于对照组（P〈0．05）。【结论】低流量持续肺动脉灌注通过减轻肺缺血再灌注损伤和粘附分子的激活来降低体外循环中的肺损伤

Iron and manganese oxides enhance electron output efficiency of Clostridium pasteurianum

[Background] Fermentative dissimilatory metal-reducing bacteria, which are capable of reducing metallic oxides, get energy from fermentation. Little is known about how metallic oxides affect electron output efficiency of fermentative dissimilatory metal-reducing microorganisms. [Objective] This study was conducted to explore the influence of iron and manganese oxides (Fe_2O_3/MNO_2) on electron output efficiency. [Methods] Different concentrations of Fe_2O_3/MNO_2 were added into fermented system containing glucose and inoculated 5% C. pasteurianum. Electrochemical activity of C. pasteurianum was detected. The concentrations of Fe(Ⅱ) and Mn(Ⅱ) were measured by ferrozine spectrophotometry and formaldoxime method. Fermentation substrate and metabolites of C. pasteurianum were detected by gas chromatography and high performance liquid chromatography. Lastly, we calculated the electron output efficiency. [Results] The current density peaked with the value of about 0.93 mA/m~2. The concentrations of Fe(Ⅱ) and Mn(Ⅱ) gradually accumulated. The consumption of glucose was increased by 9.4%/7.7%, Meanwhile, acetate production was increased by 37.5%/25.0%, and butyrate production was increased by 22.7%/6.8%. Additionally, hydrogen production was increased by 21.6%/9.8%, and the total electron output efficiency was increased by 24.27%/10.82%, respectively. The pH values between experimental group and control are no significant difference. [Conclusion] This study shows that iron and manganese oxides can improve the electron output efficiency of C. pasteurianum by increasing glucose consumption and buffering pH value. The results provide evidence for revealing the effects of multivalent metal oxides on the electron output of fermentative dissimilatory metal-reducing bacteria, and further expand our understanding of the interaction mechanism between multivalent metal oxides and fermentative dissimilatory metal-reducing bacteria.</p

基于互信息的四螺旋模型创新协同度研究

本文分析了&ldquo;政-产-研&rdquo;三螺旋模型的局限性和第四螺旋现有研究的不足之处，提出了以科技服务机构为第四螺旋构建&ldquo;政-产-研-服&rdquo;四螺旋模型的观点。随后，本文基于互信息和三螺旋算法设计了四螺旋创新协同度计量指标Tgias，并采用各主体科技论文产出数据作为其创新产出表征，利用Tgias指标量化测度了四螺旋各主体间的创新协同程度。量化分析结果表明，科技服务机构加入螺旋结构后全方位提升了政-产、政-研、产-研、政-产-研既有的协同度，反映出科技服务机构作为第四螺旋对原有三螺旋体系创新发展的催化和促进作用。对四螺旋模型的研究和应用有利于提高科技情报、技术转移等方面工作的协同创新效率。</p