我国淡水鱼类柱形病病原菌柱状黄杆菌的遗传多样性

为认识我国淡水鱼类烂鳃病的病原以及柱形病在我国的发生情况,实验从发生烂鳃病的病鱼中分离细菌性病原,经过生理生化特性分析以及是否在含托普霉素的Shieh培养基中生长并形成黄色假根状菌落,是否产生降解明胶和硫酸软骨素的酶类等特性的鉴定,并结合16SrDNA序列分析,证实柱状黄杆菌(Flavobacterium columnare)是所分离的烂鳃病的病原。同时,研究也证实20世纪曾经命名为烂鳃(Gill-rot)病病原的鱼害黏球菌(Myxococcus piscicola Lu,Nie & Ko,1975)是

Advances in the Study of Arbuscular Mycorrhizal Fungi in High Altitude and Cold Habitats on Tibetan Plateau

极端生境往往蕴藏着具有特殊生理生态功能的生物类群.海拔高、温度低、生长季节短的青藏高原高寒草原生态系统是典型的极端生境之一,目前有关该生境中分布的丛枝菌根真菌（Arbuscular mycorrhizal fungi,AMF）研究已引起广泛关注.本文从青藏高原高寒生境中AM真菌的研究概况入手,论述青藏高原高寒生境中AM真菌的群落组成、物种多样性与分布特征、菌根侵染发育状况及高寒生境对AM真菌的影响.指出高寒生境中AM真菌群落结构研究方面存在的问题,探讨解决这些问题的可能途径及其研究发展前景,以期为青藏高原高寒草原生态系统稳定性的维持与受损生态系统的恢复提供理论依据.There are many special organisms in extreme habitats. Tibetan Plateau alpine meadow e- cosystem is one of the most important extreme habitats in terrestrial ecosystem, it has the character- istics with high altitude, low temperature, and short growing season. Nowadays, the studies of ar- buscular mycorrhizal （AM） on Tibetan Plateau has been widely concerned. This review mainly dis- cussed on AM fungi communities composition, AM fungi diversity and their distribution character- istics, AM colonization feature, and abiotic factors on the effect of AM fungi. At the same time, the shortcomings about the heterogeneity of adverse habitats on AM fungi communities were pointed out for further study. Based on this, these research achievements were summarized in order to pro- vide a theoretical basis for the maintenance of the stability in Tibetan Plateau alpine steppe ecosys- tem.国家自然科学基金项目（31270558）,上海科技馆引进高层次人才科研启动经费（2015）

线性自抗扰控制参数整定鲁棒性的根轨迹分析

从极点配置和根轨迹的角度研究了二阶线性定常对象的线性自抗扰控制.提出一个新传递函数框图.基于该框图,将线性自抗扰控制的参数整定解释为闭环极点配置问题,控制器带宽、观测器带宽等参数的整定被看作开环极、零点位置的选择.建议用根轨迹法研究线性自抗扰控制的鲁棒性.通过分析根轨迹定性说明了观测器带宽可以等于控制器带宽,以及线性自抗扰控制对被控对象参数变化的鲁棒性.国家自然科学基金项目(61733017);;福建省自然科学基金项目(2016J01317);;国家留学基金项目(201606315084)资助~

内蒙古西部农牧区被动式住宅围护结构优化

为提升内蒙古西部农牧区被动式住宅室内热环境、降低采暖能耗,对具当地被动式住宅进行调研,选取了影响冬季采暖能耗的因素并进行了量化。采用能耗模拟分析软件De ST-h和正交实验的方法研究了住宅朝向、南向窗墙比、北向窗墙比、透明围护结构材质、保温层厚度、非透明围护结构材质、附加阳光间进深等7个主要因素。通过对18种组合方案的分析,以采暖能耗为评价指标得出优选组合方式。最后建立了内蒙古西部农牧区被动式住宅采暖能耗预测模型,为农牧区被动式住宅设计提供参考。研究结果能提升农牧区被动式住宅的节能设计水平。国家自然科学基金(51668051、51768053);;内蒙古自然科学基金(2016MS0516、2017MS(LH)0532);;西部绿色建筑国家重点实验培育基地开放研究基金(LSKF201803)资

Residential Thermal Comfort in Western Agricultural and Pastoral Areas of Inner Mongolia in Shoulder Season

对内蒙古西部农牧区的81户住宅的过渡季和采暖期初的室内外环境参数进行了现场测试,并以主观问卷调查的方式对居民的着装情况和热感觉等做了统计。经过对; 调查测试的结果进行了统计分析,得出内蒙古西部农牧区的的过渡季和采暖期初的热中性温度为15.6 ℃和15.3 ℃,热期望温度为17.9; ℃和18.6 ℃, 80%居民可接受温度下限为13.1 ℃和14.3 ℃, 90%的热可接受温度范围为15.9 ~ 23.3 ℃和17.5 ~; 21.9 ℃。结果表明处于当地气候条件下和穿衣、适应措施的综合影响下,农牧区居民对偏冷环境的适应性较好。The indoor and outdoor environmental parameters of 81 houses in the; western agricultural and pastoral areas of Inner Mongolia in shoulder; season and heating periods were tested.Questionnaire about the clothing; and thermal sensation was sent to residents in the houses.By analyzing; the survey results,it was found that the acceptable neutral temperature; in shoulder season and heating periods is 15.6 ℃ and 15.5; ℃,respectively; the preferred temperature in shoulder season and heating; periods is 17.9 ℃ and 18.6 ℃,respectively; the lowest temperature that; 80% residents can accept in shoulder season and heating periods is 13.1; ℃ and 14.3 ℃, respectively; and the thermal comfort temperature at which; 90% residents feel satisfied in shoulder season and heating periods; ranges from 15.9 ℃ to 23.3 ℃ and from 17.5 ℃ to 21.9 ℃,respectively.The; results show that the residents in agricultural and pastoral areas are; better adaptable to the cold environment because of the combined; influence of local climate,dressing and adaptation measures.国家自然科学基金资助项目; 内蒙古自然科学基金资助项目; 内蒙古自然科学基金资助项目; 内蒙古研究生教育创新计划资助项

Retinoic acid receptor β is required for anti-activator protein-1 activity by retinoic acid in gastric cancer cells

Objective To investigate the role of retinoic acid receptor β (RARβ) in mediating inhibitory effect of all-trans retinoic acid (ATRA) on activator protein-1 (AP-1) activity in gastric cancer cells. Methods Transient transfection and chloramphenicol acetyltransferase (CAT) assay, Northern blot, gene transfection, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, and anchorage-independent growth assay were used.Results Transient transfection of RARβ expression vector into MKN-45...theNationalOutstandingYouthScienceFoundationofChina (No 3982 5 5 0 2 )andtheNationalNaturalScienceFoundationofChina (No 39880 0 15

载体硅烷化改性对W/MCM-22催化剂上甲烷脱氢芳构化反应性能的影响

用正硅酸乙脂(TEOS)和三甲基氯硅烷(TMCS)为硅烷化试剂,分别对MCM-22分子筛进行表面改性处理,考察其对所担载W/MCM-22基催化剂上甲烷脱氢芳构化(DHAM)反应性能的影响,结果表明,TEOS或TMCS对MCM-22分子筛载体的硅烷化处理主要导致分子筛外表面最强和最弱的表面酸性位被消去,分子筛孔道内的B酸位(芳构化中心)几不受影响;在DHAM反应化学上表现为:CH4转化率大体持平,但芳烃选择性明显上升,催化剂的结焦/积碳速率有所减缓,单程操作寿命明显延长.在经硅烷化处理的8%W-0.6%Zn/MCM-22催化剂上,苯选择性最高达73.1 C%(相应甲烷转化率为15.3%),是未经硅烷化处理相应参比样(61.3 C%)的~1.2倍,单程操作寿命从300 min延长至470 min(就同等芳烃产率水平而言);类似的结果在Mo促进的W/MCM-22体系上也观察到.经硅烷化改性催化剂上可溶性积炭主要是分子量较低(在200~400范围)的饱和脂肪烃聚合物,而未经硅烷化处理参比样的可溶性积炭分子量多在500以上