丙型肝炎病毒包膜蛋白E2的原核表达及鉴定

【目的】构建丙型肝炎病毒（HCV）包膜蛋白E2基因的重组原核表达质粒，并获得E2蛋白的高效表达。【方法】通过RT-PCR法从HCV RNA阳性的血清标本中扩增出HCVE2区基因595bp，PCR产物分别经BamHⅠ和Hind Ⅲ双酶切后连接到经同样酶切的原核表达载体PET22b（＋）上，转化大肠杆菌DH5α菌株，获得阳性重组质粒PETB，阳性质粒转化BL21（DE3），IPTG诱导表达，表达产物经SDS-PAGE和Westemblot鉴定。【结果】成功地克隆了HCVE2区基因，构建了其原核表达质粒，并在BL21（DE3）菌中表达HCVE2重组蛋白，Western blot显示表达蛋白具有抗原性。【结论】HCVE2重组蛋白的表达和鉴定为进一步了解HCV糖蛋白E2的功能及其与CD81二者的相互关系打下基础

干扰素治疗后病毒性肝炎病人#br# 干扰素中和抗体的检测及意义

摘　要　使用4 种α-干扰素的98 例病毒性肝炎病人, 在治疗前、治疗期间及治疗后定期检测抗干扰素中和抗 体, 结果发现阳性率为34.7%, 该抗体的产生与病人的性别、年龄、治疗前ALT 水平、肝炎病毒感染类型、以及肝炎的 临床类型均无显著相关;低剂量干扰素较易产生中和抗体;使用不同干扰素制剂产生中和抗体存在差异, 其中以 rIFN-α2a 最高, IFN-αN1 最低;但中和抗体的出现对干扰素的抗病毒疗效影响不大, 由于病例数较少,干扰素治疗期间 出现的肝炎再活动现象与抗干扰素中和抗体产生的关系, 未能下结论

慢性肝炎肝纤维化血清学指标与肝组织病理的相关性分析

【目的】探讨肝纤维化血清学指标与病理的相关性。【方法】对191 例各种类型慢性肝病患者联合检测透明质酸 (HA)、血清Ⅲ 型前胶原(PC Ⅲ)、Ⅳ型胶原(Ⅳ-C), 同时行肝组织学检查, 进行对照研究。【结果】血清HA 水平随肝组织病理 纤维化分期而升高, 在S4 期最高, 与各期比较差异有显著性(P <0.01), 与肝组织病理纤维化分期有相关性(r =0.377, P =0.001);血清PC Ⅲ 、Ⅳ-C 水平在肝组织病理纤维化分期各期中无差异(P >0.05);血清HA 、Ⅳ-C 水平随组织病理炎症活 动度分级而升高, 在G4 时最高, 与各级比较差异有显著性(P 0.05)。【结论】血 清HA 水平与肝组织病理学的分级和分期的相关性较好, 可作为一种无创伤的肝纤维化诊断指标, 而血清PC Ⅲ 、Ⅳ-C 水平与 肝组织病理学的分级和分期的相关性不明显, 对肝纤维化诊断的意义还需进一步研究

Prediction of Energy Resolution in the JUNO Experiment

International audienceThis paper presents the energy resolution study in the JUNO experiment, incorporating the latest knowledge acquired during the detector construction phase. The determination of neutrino mass ordering in JUNO requires an exceptional energy resolution better than 3% at 1 MeV. To achieve this ambitious goal, significant efforts have been undertaken in the design and production of the key components of the JUNO detector. Various factors affecting the detection of inverse beta decay signals have an impact on the energy resolution, extending beyond the statistical fluctuations of the detected number of photons, such as the properties of liquid scintillator, performance of photomultiplier tubes, and the energy reconstruction algorithm. To account for these effects, a full JUNO simulation and reconstruction approach is employed. This enables the modeling of all relevant effects and the evaluation of associated inputs to accurately estimate the energy resolution. The study reveals an energy resolution of 2.95% at 1 MeV. Furthermore, the study assesses the contribution of major effects to the overall energy resolution budget. This analysis serves as a reference for interpreting future measurements of energy resolution during JUNO data taking. Moreover, it provides a guideline in comprehending the energy resolution characteristics of liquid scintillator-based detectors