Calcium Distribution in the Sac of Leek (A. tuberosum Rottl. ex Spreng.) during development

Ca2+与高等植物的有性生殖过程密切相关，无论是大、小孢子发生、雌、雄配子体发育，还是传份、受精、胚胎发生等过程，Ca2+都在其中发挥着重要的作用。已有研究表明，花药发育过程中普遍存在Ca2+的特异动态变化；体外培养的花粉管还具有向高浓度Ca2+生长的特性；在雌性组织中继续有关Ca2+分布特征的探索是证实花粉管向钙性的关键。但目前为止，有关这方面的研究还不多，主要集中在开花前后胚珠中的Ca2+与花粉管定向生长的关系方面，而有关胚囊发育过程中Ca2+分布变化的研究却很少。 本课题选韭菜为实验材料，主要考虑到韭菜具有助细胞二型性、孤雌生殖、无配子生殖的特点，对其胚囊中Ca2+特征变化的探索具有多...Ca2+ is closely related to sexual reproduction of higher plant. Ca2+ plays an important role whether in the process of microsporogenesis, megasporogenesis, the development of the male and female gametophyte, or in the process of pollination, double fertilization and embryogenesis. Studies have shown that, dynamic changes of Ca2+ generally exist in the anthers’ development, and the pollen tubes in ...学位：理学硕士院系专业：生命科学学院_发育生物学学号：2162012115230

The Character of Anther Development of Impatiens balsamina

凤仙花花药发育比较特殊:在造孢细胞时期,花药横切面中央是体积较大、细胞内含物较多的细胞团、包括造孢细胞和绒毡层细胞。花药药壁细胞的细胞质较稀少,与中部细胞界限明晰。花粉母细胞时期的花药药壁由约6层细胞组成,但细胞的界限不明显;绒毡层细胞显示变形流入药室中。到四分体时期,绒毡层细胞进一步退化。开花时,成熟花药的药壁细胞由一层表皮细胞、两层药室内壁细胞和一层中层细胞组成。对凤仙花花药绒毡层的特殊性质进行了讨论。The anther development of the Impatiens balsmina displayed some special characters.In sporogenous cell stage, there are some large sporogenous cells, containing density cytoplasm, locating in the central of anthers.The cells of anther wall contain less cytoplasm which makes both distinguish.In the stage of microspore mother cell, the anther wall consists of 6 layers of cells, but the limit line is not clear between the cells.The tapetal cells begin to display the degeneration at this time.In tetrad stage, the tapetal cells degenerate further as the cells become small.The mature anther wall consists of four layers of cells: one layer of epidermis, two layers of endothecium, and one layer of middle layer.The special characters of tapetal cells of Impatiens balsamina were discussed.国家自然科学基金(31170289;31100225); 公益性行业(农业)科研专项课题(200903016)资

The Characteristic of Calcium Distribution in Developing Female Gametophyte of Allium tuberosum

对韭开花前1 d到开花后2 d的发育胚囊中CA2+分布特征进行研究,结果显示:在开花前1 d游离核胚囊形成细胞,CA2+主要分布在极核周围的细胞质小液泡中,与中央细胞的大液泡形成有关;在开花当天,卵细胞大液泡体中CA2+增多,与其大液泡形成有关;开花后1 d,卵细胞大液泡中钙沉淀颗粒减少,助细胞珠孔段的CA2+增多,合点端较少,呈极性分布;开花后2 d,卵细胞大液泡和中央细胞大液泡珠孔部位CA2+再次明显增加。助细胞珠孔端丝状器中CA2+也增加,一个助细胞退化,呈现接受花粉管状态。韭发育胚囊中CA2+分布特征显示其参与调控胚囊细胞的分化,揭示雌配子体中CA2+分布的时空特征是深入研究胚囊细胞分化的基础。Characteristic of Ca2+ distribution in developing female gametophyte of Allium tuberosum was studied from 1 d before anthesis to 2 d a fter anthesis.At 1 d before anthesis, the nuclear embryo sac differentiates gametophyte cells.Calcium precipitates of young embryo sac were few and mainly located in the small vacuoles surrounding polar nuclei, suggesting Ca2+ relating with the formation of the large vacuole of central cell.At anthesis, the precipitates in large vacuole of egg increased, also suggesting Ca2+ relating with formation of the large vacuole of egg cell.At 1 d after anthesis, the precipitates in egg cell decreased and increased in synergid cells, in which the precipitates displayed polarity distribution: more in the micropyle end of the cell than in the chalazal end.At 2 d after anthesis, the precipitates in the large vacuole of egg and the micropyle part of central cell appeared again.The precipitates in filiform apparatus of both synergids also increased.One synergid cell degenerated, which prepared to accept pollen tube.The characteristic of calcium distribution in developing embryo sac of A.tuberosum suggests that Ca2+ may regulate cell differentiation of embryo sac.Exploring the spatial-temporal feature of Ca2+distribution in developing embryo sac is a base of further study of cell differentiation of embryo sac.国家自然科学基金(31170289); 公益性行业(农业)科研专项课题(200903016

The Primary Attempt of in Vitro Fertilization of Torenia fournieri

通讯作者(E-mail: [email protected]; Tel: 0592-2186486)。[中文文摘]用体内-体外方法分离了蓝猪耳精细胞。用酶解和解剖方法分离了其成熟卵细胞。分离的精、卵细胞用电融合介导尝试了体外诱导融合。在合适的渗透压(6%甘露醇)和合适的氯化钙(0.04%CaCl2·2H2O)溶液中,用交流电场为30～35V,10～25s使精、卵细胞排队;用直流电场400～600V,45～50μs的脉冲穿孔条件可诱导30%的精、卵细胞融合和70%以上的卵细胞之间的融合。尝试了人工合子的单细胞培养但未获成功。诱导蓝猪耳精、卵细胞融合的条件与玉米和水稻不同。[英文文摘]Sperm cells of Torenia fournieri were isolated from pollen tubes which were induced using an in vivo-in vitro method. Its egg cells were isolated by enzyme digesting followed by mechanical dissection. The electrical fusion method was applied to induce the fusion of the isolated sperm and egg cells. In a suitable solution containing 6% mannitol and 0.04% CaCl2 ·2H2O, 30% sperm-egg and over 70% egg-egg could be induced fusion using alternating current (30–35 V, 10–25 s) to align sperm-egg or egg-egg,following direct current (400–600 V, 45–50 μs) pulse to perforate the plasma member of both sperm and egg. The artifi cial zygotes were also tried to culture but no success. The conditions of inducing fusion of sperm cells and egg cells of Torenia fournieri are different with those of maize and rice.国家自然科学基金(30970275和31170289);; 公益性行业(农业)科研专项经费(200903016

类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家

A JIT Planning Approach for Crude Oil Procurements in Distributed Supply Chain

本文研究了分布式供应链多供应商/多炼油厂的原油采购计划问题,建立了在有限资源约束下的准时制多目标采购优化数学模型,以实现总采购费用和供应链循环时间最小。然后,本文将非线性规划模型转化为线性规划模型,并利用层次分析法(AnalyticHierarchyProcess,AHP)与多目标规划相结合的方法求解模型得到采购计划方案。数值仿真结果表明本文所提出的原油采购方法的有效性和实用性

Application Research of AVM in Mass Appraisal Systems of Foreign Countries

摘要： AVM（自动评估模型）是批量评估方法体系的关键技术。本文系统地介绍了AVM的技术原理，并论述了模型设定理论层面与模型校准技术层面以及两者相互的联系，提出了开发和应用国内AVM，建立税基批量评估方法体系的一些构想。 Automated valuation model (AVM) is the key technique of mass appraisal system. This paper reviews the development of AVM, introduces systemically the technical principle of AVM and demonstrates how to specify and calibrate an AVM and their relationship. Finally, basing on China’s reality and the other countries’ AVM experience in assessment, the authors discuss how to develop and apply AVM in assessment and build up mass appraisal system in China

国外AVM技术在批量评估中的应用

AVM(自动评估模型)是批量评估方法体系的关键技术。本文系统地介绍了AVM的技术原理,并论述了模型设定理论层面与模型校准技术层面以及两者相互的联系,提出了开发和应用国内AVM,建立税基批量评估方法体系的一些构想

A Survey of Supplier Selection Models and Approaches

供应商选择问题是学术界和工业界十分关注的优化决策问题,许多研究者进行了并且还在继续进行相关的研究工作。本文采用分类的方法归纳了供应商选择问题的研究进展,深入分析了在不同采购策略下供应商评价准则、供应商选择模型和优化方法。本文指出了各种研究方法的优缺点、适用范围、存在的问题,并且进一步指出未来研究的方向