3,828 research outputs found
Coupling between the laryngeal and supralaryngeal systems
Includes bibliographical references (p. 27-30)."A dissertation submitted in partial fulfillment of the requirements for the Bachelor of Science (Speech and Hearing Sciences), The University of Hong Kong, June 30, 2010."Thesis (B.Sc)--University of Hong Kong, 2010.The present study investigated the coupling between the laryngeal and supralaryngeal
systems in speech production. The interrelationship between the two systems was examined
by studying the possible interaction between tone production (laryngeal system) and
articulation (supralaryngeal system). Sixty (30 male and 30 female) native Cantonese
speakers participated in the study. The first and second formant frequencies (F1 and F2)
associated with the four vowels /i, u, ?, ?/ produced at six Cantonese lexical tones (highlevel,
high-rising, mid-level, low-falling, low-rising and low-level tones) were obtained.
Results revealed that, regardless of vowels, significant articulatory changes were found when
produced at different tones. However, the difference pattern across each vowel was not
systematic. Gender difference was also noted; male and female speakers showed different
patterns in articulatory changes. These findings revealed the coupling effect between the
laryngeal and supra-laryngeal systems.published_or_final_versionSpeech and Hearing SciencesBachelorBachelor of Science in Speech and Hearing Science
中国科协主管科技期刊经营状况研究
中国科协目前主管科技期刊441种。本文通过问卷调查方式,获取了中国科协主管科技期刊的基本发展现状、出版发行、广告经营、数字化发展及多种经营等方面的基础数据。调查显示,中国科协主管科技期刊以非法人出版单位为主,主要的经营模式为单刊发展,在经营管理中较为重视经营人才。本文指出了中国科协科技期刊在经营中存在的一些问题并提出了相应的建议。</span
Expression of organophosphorus-degradation gene(opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria
Genetic engineering in filamentous N2-fixing cyanobacteria usually involves Anabaena sp.PCC 7120 and several other non-aggregating species.Mass culture and harvest of such species are more energy consuming relative to aggregating species.To establish a gene transfer system for aggregating species,we tested many species of Anabaena and Nostoc,and identified Nostoc muscorum FACHB244 as a species that can be genetically manipulated using the conjugative gene transfer system.To promote biodegradation of organophosphorus pollutants in aquatic environments,we introduced a plasmid containing the organophosphorus-degradation gene(opd) into Anabaena sp.PCC 7120 and Nostoc muscorum FACHB244 by conjugation.The opd gene was driven by a strong promoter,PpsbA.From both species,we obtained transgenic strains having organophosphorus-degradation activities.At 25°C,the whole-cell activities of the transgenic Anabaena and Nostoc strains were 0.163±0.001 and 0.289±0.042 unit/μg Chl a,respectively.However,most colonies resulting from the gene transfer showed no activity.PCR and DNA sequencing revealed deletions or rearrangements in the plasmid in some of the colonies.Expression of the green fluorescent protein gene from the same promoter in Anabaena sp.PCC 7120 showed similar results.These results suggest that there is the potential to promote the degradation of organophosphorus pollutants with transgenic cyanobacteria and that selection of high-expression transgenic colonies is important for genetic engineering of Anabaena and Nostoc species.For the first time,we established a gene transfer and expression system in an aggregating filamentous N2-fixing cyanobacterium.The genetic manipulation system of Nostoc muscorum FACHB244 could be utilized in the elimination of pollutants and large-scale production of valuable proteins or metabolites
Design and Implementation of Secure File Interchange System
随着信息化的不断发展,各行业大都使用不同开发语言、基于异构软硬件平台建成了许多成熟的业务信息系统,这些系统每天产生大量的业务数据。为了充分利用这些数据资产,各系统间需要进行数据交换,实现企业数据共享。 在此背景下,本文提出了一种基于平面文件、安全、轻量级、通用且近于零成本的数据交换平台:安全文件交换系统(SFIS)。通过采用统一的数据交换标准,各应用系统与基于分布式运行的SFIS相连,来实现数据共享。应用具有自主知识产权和高安全强度的国密算法SM3和SM4,采用安全的密码协议保障文件的传输安全;通过文件传输成功完成、时间到达、作业完成等条件触发事件,通知调度引擎,实现无轮询作业调度;调度引擎...With the continuous development of information technology, most enterprises have applied different development languages, heterogeneous software and hardware platform to build many mature business information systems. These systems produce large amounts of business data. In order to make full use of these data assets for realizing enterprise data sharing, the systems need to carry on the data inte...学位:工学硕士院系专业:软件学院_软件工程学号:X201323024
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