Determination of DNA using Rivanol as the Fluorescent Probe in the Presence of SDS

建立了在SdS存在下， 以利凡诺（rVn） 为荧光探针测定dnA 的新方法. 在适量SdS存在下， rVn 的荧光被强烈地猝灭直至最低值. 但于上述rVn- SdS体系中加入dnA 后， 体系的荧光强度增强， 且荧光光谱发生移动. 在一定条件下， 荧光强度增强值与加入dnA 的量在一定浓度范围内呈现良好的线性关系.The quantitative method for DNA using rivanol(RVN) as the fluorescent probe in the presence of SDS was proposed.Under proper conditions,addition of DNA to a mixture of RVN and SDS resulted in enhanced fluorescence and spectral shifts of RVN-SDS system.The calibration graph was linear in the range of 0～10 μg/mL,the limit of detection was 62 ng/mL CT DNA

仙女堡水电站桩基承载力有限元分析研究

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深圳福田白骨壤红树林生物量和能量研究

本文探讨了深圳福田红树林保护区５６年生白骨壤群落的生物量、生长量及能量分布。结果表明：该群落现存生物量为１６５８３８８ｇ／ｍ２，其中地上部占６６７８％，地下部占３３２２％。年增长量（１９９３）为１１４９８ｇ／ｍ２。植物体各组分热值含量变化范围在１６１～１８２２ＫＪ／ｇ，其中以树干材热值含量居最高，而中根最低；群落能量现存量为２９１８３９ＫＪ／ｍ２，其中地上部占６８５１％，地下部占３１４９％；群落年增长能量存留量（１９９３）为２０２１３ＫＪ／ｍ２

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聚苯乙烯接枝聚甲基丙烯酸甲酯共聚物的合成新方法①郭金全邹友思戴李宗袁亿文潘容华（厦门大学化学系厦门３６１００５）阴离子聚合［１］和基团转移聚合（ＧＴＰ）［２］技术可分别使非极性单体和极性单体进行活性聚合，得到分子量分布窄、实际分子量和设计分子量相近的..

EGCG阻断HDAC6-PI3K/AKT/mTOR轴以激活自噬促进小胶质细胞清除Aβ

目的为明确表没食子儿茶素没食子酸酯（EGCG）是否参与小胶质细胞对β-淀粉样蛋白（Aβ）的清除和自噬诱导，以探讨EGCG在防治阿尔茨海默病（AD）的潜在机制。方法将6月龄APP/PS1小鼠随机分为模型组和EGCG组，另将野生型小鼠作为对照组，每组15只，EGCG组持续灌胃给药［5 mg/（kg·d）］8周后，进行旷场实验及Y迷宫检测小鼠学习记忆能力，硫磺素-S染色评价小鼠脑实质中Aβ的含量及分布，免疫荧光检测小鼠海马组织Aβ1-42、胶质纤维酸性蛋白（GFAP）、离子钙结合适配器分子1（Iba1）表达水平；同时予20 µmol/L Aβ1-42诱导N9小鼠胶质细胞模型，检测不同浓度EGCG（5 µmol/L、10 µmol/L、20 µmol/L）处理后的细胞活力，Western blot检测Aβ1-42、低密度脂蛋白受体相关蛋白1（LRP1）、晚期糖基化终末产物受体（RAGE）、淀粉样前体蛋白（APP）、胰岛素降解酶（IDE）、脑啡肽酶（NEP）、微管相关蛋白1轻链3（LC3）-Ⅱ/LC3-Ⅰ、磷脂酰肌醇-3-羟激酶（PI3K）、p-PI3K、蛋白激酶B （AKT）、p-AKT、哺乳动物雷帕霉素靶蛋白（mTOR）、p-mTOR、组蛋白去乙酰化酶6（HDAC6）水平；最后通过小胶质细胞与神经元SH-SY5Y细胞共培养，检测细胞活力及Caspase-3水平，验证EGCG介导Aβ清除对神经元的保护作用。结果EGCG增加APP/PS1小鼠在旷场中央区域活动时间及次数（P＜0.05），提高Y迷宫测试交替百分比（P＜0.01）；EGCG减少APP/PS1小鼠海马组织中Aβ沉积，增加小胶质细胞数量；体外实验显示EGCG可提高Aβ诱导N9细胞的存活率（P＜0.01），并上调RAGE活性（P＜0.05），促进Aβ的内化吞噬（P＜0.01），通过下调HDAC6水平（P＜0.05），抑制PI3K、AKT、mTOR的磷酸化（P＜0.001）而增加LC3-Ⅱ/LC3-I比值（P＜0.001）激活小胶质细胞自噬；EGCG通过小胶质细胞对Aβ1-42的清除，提高SH-SY5Y细胞存活率（P＜0.05），降低Caspase-3的活性（P＜0.01），对神经元具有保护作用。结论EGCG通过靶向阻断HDAC6-PI3K/AKT/mTOR轴，激活小胶质细胞自噬清除Aβ

ANALYSES ON LDH ISOENZYME AND PROTEIN FROM BOTH GUANGDONG STRAIN AND FUJIAN STRAIN OF ECHINOCHASMUS FUJIANENSIS

目的　探讨福建与广东两地福建棘隙吸虫生化特点。方法　用不连续聚丙烯酰胺凝胶电泳及薄层等电聚焦电泳等进行比较分析虫体乳酸脱氢酶 (LDH)同工酶和蛋白质。结果　福建与广东两地虫株都有 5种LDH同工酶 ,其中LDH1、LDH4 和LDH5的迁移率相同 ;蛋白质电泳都分离出 9条区带 ,其中有 7条带的等电点相同。但在LDH2 和LDH3 的迁移率 ,蛋白质区带的相对含量 ,同工酶的总活力与比活力 ,以及A和B亚基的含量等存在差异。结论　LDH同工酶与蛋白质的某些差异 ,可归因为基因型相似条件下所出现两地虫株生化遗传上差异Aim\ Inguired into the biochemical characteristrics of Echinochasmus fujianensis found in Fujian and Guangdong provinces. Methods\ Compared and analysed two strains by LDH isoenzyme eletrophoresis, discontinuous PAGE and thinlayer isoelectric equilibrium PAGE.Results\ Two strains both had five LDH isoenzyme, among them, LDH\-1、LDH\-4 and LDH\-5 had the same mobilities. There were nine bands in protein electrophoresis. Seven bands had the same isoelectric point.But there were differences in the mobilities of LDH\-2 and LDH\-3,the comparative amount of protein bands, total and specific activity of isoenzyme and the amount of A and B subunit. Conclusion Under the similar condition of genotypes, the differences of the biochemical inheritance can be contributed to the differences of LDH isoenzyme and protein.福建省科技计划基金资助项目!(No.98-Z-156);; 福建省“百千万人才”人选培养资金资助项

SURVEY OF MIXED INFECTIONS OF FISH-BORNE TREMATODES IN PINYUAN COUNTY AND DNA(RAPD)ANALYSIS

目的　调查广东省平远县棘隙吸虫及其他鱼源性吸虫混合感染情况。方法　对鱼类宿主取鱼鳃和鱼肉等组织 ,镜检囊蚴 ;用水洗过筛沉淀法粪检虫卵 ,部分虫卵阳性动物解剖检查。采用DNA -RAPD技术 ,比较福建棘隙吸虫广东株与福建株。结果　查出华支睾吸虫 (Clonorchissinensis)、东方次睾吸虫 (Metorchisorientalis)、福建棘隙吸虫 (Echinochasmusfujianensis)、日本棘隙吸虫 (E japonicus)、抱茎棘隙吸虫 (E perfoliatus)和钩棘单睾吸虫 (Haplorchispumilio) 6种。第二中间宿主为麦穗鱼等 11种 ,感染率 48 3% (182 / 377) ,有 92 4%的阳性鱼混合感染 2种以上吸虫囊蚴。保虫宿主狗、猫感染率 79 2 % (19/ 2 4) ,每只阳性动物均有 2种以上吸虫混合感染。 15个引物在福建棘隙吸虫广东株与福建株两者共获DNA片段 185个 ,共享度 (F) =0 984,遗传距离 (D) =0 0 16。结论　当地为人兽共患的多种鱼源性吸虫混合感染区。形态学观察和分子生物学检测结果相印证 ,福建棘隙吸虫广东株与福建棘隙吸虫福建株为同一虫种。Aim To seek the possible existence of mixed infections of Echinochasmus fujianensis and other fish-borne tremtodes in Pinyuan county,Guangdong.Methods Metacercariae of trematodes were detected from gills,muscles and some other tissues of freshwater fish and examintd under microscopy Sedimentation pocedures were used to observe parasite ova in stool Adult worms were obtained from definitive animal hosts upon dissection Experimental animal infection were performed from metacercariae of E fujianensis strains in Fujian and Guangdong,and it's DNA analysis Random amplified Polymorphic DNA(RAPD).Results Six species of trematodes were found,including Clonorchis sinensis,Metorchis orientalis,E fujanensis, E japonicus,E perfoliatus and Haplorchis pumilio Eleven species of freshwater fish comprising Pseudorasbora parve were identified as the second intermediate hosts,with an average positive rate of 48 33%(182/377) The overall mixed infection animal were contracted with 2 or more species Dogs and cats were detected to be reservoir hosts,the total infection rate being 79 2%(19/24).92 4% of infecded fish were contracted with 2 or 6 speies of the metacercariae of trematodes 185 DNA fragments were obtained by 15 primers,F=0 894 and D=0 016 between E fujianensis Guandong and Fujian strain Conclusion There are six species of fish-borne trematodes which may cause infections in human and animal in the regions E fujianensis Guangdong stain and Fujian strain are the same species福建省科技计划基金!资助项目 (No 98-Z - 1 56);; 福建省“百千万人才”人选培养资