Design and Implementation of Jiang Xi Ping xiang Pubic Security Intelligence Information Management System

伴随着信息技术和计算机技术的快速更新，社会经济逐步提升，人们生活质量得到较大提高，可是目前社会大众的精神需求和社会文化发展具有较大差距，于是随之而来的是不同层面的犯罪案件的出现，与此同时，江西萍乡市对情报信息的管理依然采取以纸质管理为主。针对目前存在的问题，江西萍乡市公安局为解决自身存在的问题的同时，力求改变这种局面，设计并实现了一套江西萍乡市公安情报信息管理系统，为江西萍乡市公安部门带来一个基于网络的协同管理平台，能够实现情报管理、情报分析、情报查询、警情预警等多功能一体的案件管理平台。 本论文首先介绍了目前国内外情报信息管理系统，并结合本人学习研究的先进软件技术和情报信息管理的主要业务流...With the rapid development of information technology and computer technology, the social economy has gradually improved, people's quality of life has been greatly improved, but the spiritual needs of the current social and cultural development of the community with a large gap, then followed by different levels of criminal cases arise, at the same time, the management of intelligence information, ...学位：工程硕士院系专业：软件学院_软件工程学号：X201223080

Credit Risk Measuring Models of the Listed Companies ——An Empirical Research on Default Probability

在全球信用不断膨胀的背景下，信用风险暴露越来越严重，已成为各国金融系统所面临的主要风险之一。如何有效地控制和管理信用风险已经成为各国金融监管当局、金融机构和投资者关注的焦点。在这样的国际金融背景下，学习和借鉴国际先进的信用风险计量技术，建立在中国行之有效的信用风险计量和管理模型，提高我国风险管理水平，是金融界面临的最大挑战之一。由此，本文对我国上市公司的信用风险计量及其相关问题做出研究。 本文分析了信用风险的概念和特征，着重研究了信用风险的计量方法，力求通过研究找到适合我国实际情况的上市公司信用风险计量模型，从而增强我国信用风险测定与管理的能力。首先简单介绍了传统的“5C”专家法、信用评分和...With the rapid growth of credit in today＇s global economy， problems related to credit risk have attracted much attention. Credit risk has become one of the important risks which financial institutions have to face. For that，How to control and get an accurate estimation of credit risk plays a key role in the decision making of financial intermediaries、 investors and government supervisor .So It bec...学位：经济学硕士院系专业：经济学院金融系_金融学(含保险学)学号：X200614400

A Study on the Regional Selection of Multinational Corporations’ R & D Investment in China

随着经济全球化的深入发展，跨国公司研发组织的模式发生了重大变化，突破了将研发机构保留于本国的传统格局，从自身的全球经营战略出发，不断在海外建立科研机构。我国自改革开放以来，经济保持着高速增长，跨国公司也高度重视我国市场的战略地位，不仅提高了在我国市场的投资，也加大了在我国的研发投资。但是我国不同的省份之间存在着很大的差异性，在经济水平、科技水平、人才素质、基础设施、产业配套等方面发展不平衡，跨国公司在我国进行研发投资时，应充分考虑这些因素，从而做出正确的区位选择决定。 本文首先从跨国公司海外研发投资区位选择的基本理论出发，在分析跨国公司在我国研发投资的现状的基础上，对跨国公司在我国研发投资区...With the deepening of economic globalization, multinational R&D organization model has undergone major changes. Breaking the traditional pattern which remained in the national R&D institutions, from its global business strategy, Multinational Corporation constantly establishes research institutions overseas. Since the reform and opening up, China's economy has maintained a rapid growth. Multinatio...学位：国际商务硕士院系专业：经济学院_国际商务学号：1572011115183

基于隐因子模型的P2P借贷推荐算法

P2P网络借贷的兴起使得网络借贷平台上投资用户数目以及贷款项目数目迅速激增,同时带来海量的借款人信息以及贷款信息,投资用户在众多贷款项目中找到自己感兴趣的贷款项目变得愈加困难。本文在研究推荐系统相关算法的基础上,详尽的实验了基于隐因子模型的推荐算法,在挖掘用户感兴趣的贷款的准确率。为了说明算法的有效性,本文做了相关推荐算法的多组对比实验。对比实验结果表明,基于隐因子模型的推荐算法能够更有效地预测投资用户的投资偏好,并且对推荐系统应用到P2P借贷平台具有借鉴意义

Expression and purification of mucin 16 and preparation and characterization of anti-mucin 16 monoclonal antibody

目的:在原核生物中表达带有HIS标签的MuCIn 16n端重组蛋白(简称为HIS-MuCIn 16n),制备抗MuCIn 16的单克隆抗体(MAb)。方法:将MuCIn 16基因片段插入原核表达载体PET-32,在大肠杆菌中表达重组蛋白,用亲和纯化方法纯化后免疫bAlb/C小鼠,并进行细胞融合。筛选可稳定分泌抗MuCIn 16抗体的阳性单克隆杂交瘤细胞株,用WESTErnblOT、ElISA、免疫荧光和免疫组化等方法分析和鉴定抗MuCIn16的MAb。结果:表达并纯化了HIS-MuCIn 16n蛋白;筛选出几株可稳定分泌特异性抗人MuCIn 16 MAb的细胞株;挑选出效价高、特异性好的1株进行纯化。获得的抗MuCIn 16 MAb,可用于WESTErn blOT、ElISA、免疫组化、免疫荧光等检测,并鉴定该抗体亚型为Igg1。通过上述免疫学实验,分析了在不同肿瘤细胞中MuCIn 16的表达情况。结论:在原核生物中成功表达和纯化带HIS标签的MuCIn 16n重组蛋白,制备出具有高特异性的抗MuCIn16的MAb。AIM:To generate monoclonal antibodies(mAbs) against mucin 16 using purified recombinant protein of human mucin 16 N terminus with His tag(His-mucin 16N) as the antigen.METHODS: Mucin 16 N terminus was cloned into a prokaryotic expression vector pET-32.His-mucin 16N was then expressed in E.coli and purified by the affinity chromotography.Cell fusion was performed after the BALB/c mice were immunized with the purified His-mucin 16N protein.We screened hybridoma cell strains producing mAbs against mucin 16.The specificity and titer of the antibodies were characterized with ELISA,Western blotting,immunofluorescent and immunohistochemical staining.RESULTS: The recombinant protein of His-mucin 16N was expressed and purified.A few hybridoma cell strains which could secrete specific mAbs against mucin 16 were obtained,and one anti-mucin 16 mAb with good specificity and high titer was selected and purified.The isotype of this anti-mucin 16 mAb was determined as IgG1,which indicated that this anti-mucin 16 mAb could be used for ELISA,Western blotting,immunofluorescent and immunohistochemical staining.The endogenous expression of mucin 16 in various cancer cell lines or tissues was also examined with this anti-mucin 16 antibody by Western blotting and other immunoassays.CONCLUSION: The recombinant protein of His-mucin 16N was expressed and purified successfully,with which we prepared anti-mucin 16 mAb with good specificity and high titer.福建省科技重点项目(2011Y0050);厦门市科技计划创新项目(3502Z20123009

Characterization of NSE monoclonal antibodies and establishment of a double-antibody sandwich ELISA assay

通讯作者，E-mail: xtli@ xmu.edu.cn 作者简介: 丁焕弟( 1985 年－ ) ，女，在读硕士，主要从事抗肿瘤单克 隆抗体及诊断试剂盒的研究，E-mail: dinghuandi1125 @163.com。[中文文摘]目的:制备并鉴定NSE(Neuron-specific enolase)单克隆抗体,建立可检测NSE蛋白的双抗夹心ELISA方法。方法:用本实验室已表达纯化的NSE融合蛋白免疫BALB/c小鼠,采用杂交瘤技术制备单克隆抗体。采用WB、IP、IF、IHC等方法对获得的NSE单抗进行鉴定及亚型检测。利用辣根过氧化物酶标记纯化后的NSE单抗,建立一个可检测NSE蛋白的双抗夹心ELISA法。结果:通过分析和鉴定,选定2株可稳定分泌抗NSE抗体的杂交瘤细胞株,效价达4.2×107～6.5×107,亚型为IgG2b。免疫印迹结果显示,该抗体不仅能识别细胞内源NSE蛋白,还能识别分泌到细胞培养上清液中的NSE蛋白,此外还可用于免疫荧光及免疫组化检测。文中所建立的双抗夹心ELISA法,最低检测极限为8.85 ng/ml。结论:成功获得了效价高、灵敏度好及特异性强的NSE单抗,建立了一个双抗体夹心ELISA检测系统,具有良好的临床应用前景。[英文文摘]Objective: Preparation and characterization of monoclonal antibodies against NSE protein，and establishment of a double-antibody sandwich ELISA assay． Methods: BALB/c mice were immunized by using purified recombinant NSE，and monoclonal antibodies were generated by hydridoma technique． These antibodies were characterized with ELISA，Western blot，Immunofluorescent and Immunohistochemical staining． The isotypes of these antibodies were determined with an antibody isotyping kit． With Horseradish Peroxidase labelled NSE monoclonal antibody，we were able to establish a double-antibody sandwich ELISA to detect NSE protein． Results: Two positive hybridoma cell lines were selected for test，the titers of these two monoclonal antibodies could reach 4． 2 × 107 －6. 5 × 107，and their isotypes were IgG2b． Our NSE antibodies could detect not only endogernous NSE protein from cells，but also secreted NSE protein from cells in culture medium by Western blot，in addition，they could be used for immunofluorescent and immunohistochemical staining． The minimum amount of NSE protein could be detected by this double-antibody sandwich ELISA was 8． 85 ng /ml． Conclusion: Our NSE monoclonal antibodies achieved good sensitivity and specificity with high titers，and we established a doubleantibody sandwich ELISA assay which could be used for clinical test in future．福建省科技重点项目(编号项目No.2011Y0050); 厦门市科技计划项目(No.3502Z20123009

掺Yb~(3+)双包层光纤激光器的理论及实验研究

文中报告了掺Yb3+双包层光纤激光器的理论和实验研究工作。在稳态条件下,基于速率方程推导了掺Yb3+双包层光纤激光器在强泵浦条件下的简化型解析解。实验上,利用975 nm大功率半导体激光作为泵浦源,采用单端泵浦技术,获得了88 W波长为1 082.4 nm的连续激光输出,斜效率高达84.4%。实验结果与理论的简化型解析解相比较,两者基本一致

中国心理学会会议论文集

以牺牲自身利益为代价遵循社会规范,是人类独特的特征。然而,在特定情境下,人们这种遵循社会规范的行为会发生偏离。比如,在最后通牒游戏中,面对高的分配金额,对不公平分配方案的拒绝率会降低。但这种现象背后的神经机制仍不清楚。在本研究中,我们采用了一种修订的最后通牒游戏范式分离了公平水平和分配金额的效应,以考察分配金额影响互惠公平的神经基础。在行为学水平上,我们验证了分配金额对分配方案拒绝率的调节作用,即&nbsp;</p

公平的神经基础

公平问题是人类长久以来的话题,研究已经证实人们存在着公平偏好。关于公平的神经基础,研究者采用脑功能成像技术进行了深入探索,并提示了其在精神疾病研究中的应用价值。本文介绍研究公平行为的博弈实验范式&mdash;&mdash;最后通牒博弈,概述基于最后通牒博弈的神经影像学研究发现。从分配额度、得失情境、框架效应、群体意见、社会地位及情绪几个方面,综述影响公平行为的因素及其神经基础。在临床研究方面,列举了有关抑郁症、精神病态患者及反社会青少年的公平行为及其神经基础的相关研究。分析表明,未来研究应注意从脑网络的角度对公平行为的神经基础进行探讨,考虑到具体的社会情境对公平行为的影响,加强博弈实验范式在神经精神疾病中的应用,并深入探索公平感知的神经计算模型