腺病毒介导血管内皮细胞生长因子基因体外转染骨髓间充质干细胞

【目的】探讨腺病毒介导血管内皮细胞生长因子(VEGF165)基因转染大鼠骨髓间充质干细胞(MSCs)后目的基因表达情况及对MSCs增殖分化的影响。【方法】构建含VEGF基因的腺病毒表达载体,利用贴壁法分离培养MSCs后,用荧光显微镜和流式细胞仪检测转染效果和转染率,用免疫组化、Western-Blot和ELISA方法分别检测VEGF基因转染MSCs后VEGF的表达情况。【结果】腺病毒介导的VEGF基因对于MSCs具有很高的转染效率,转染效率与病毒感染复制数(multiplicyties of infection,MOI)具有量效关系。MOI为150倍时,转染效率>95%,转染VEGF后,MSCs可有效表达VEGF,9d时达到表达高峰(1125pg/mL),13d后仍可检测到VEGF的表达。转染VEGF对MSCs的增殖分化没有明显影响。【结论】腺病毒介导的VEGF基因可以有效的转染MSCs,MSCs是一种理想的基因载体细胞,其携带的VEGF基因可获得较高的表达水平

基于栖息地风险评价模型的海岸带滩涂湿地风险评价——以闽三角为例

海岸带湿地是生物多样性最丰富、生产力最高、最具价值的生态系统之一。然而近年来,随着城市化和工业化进程的加快,我国海岸带地区的土地利用正发生深刻变化,生态风险凸显。利用2000、2005、2010和2015年的Landsat TM/ETM+和Landsat 8 OLI遥感影像,融合行政区划图与地形图,并引入一个评价多元海洋、海岸带利用对栖息地造成风险的模型,即Integrated Valuation of Ecosystem Services and Trade-offs(In VEST)模型中的栖息地风险评价(Habitat Risk Assessment,HRA),模型评价闽三角海岸带滩涂湿地的生态风险。结果表明:(1)闽三角海岸带滩涂湿地风险以低风险为主;(2)泉州地区的风险面积最大(约4389.91 hm~2),漳州地区最小(约4630.73 hm~2);(3)修正面积影响的情况下,其他建设用地造成的暴露和影响较大,耕地造成的暴露和影响最小。不同区域滩涂湿地风险程度的可视化表达,可以揭示滩涂湿地高风险地区以及高风险的产生原因,便于管理者对滩涂湿地采取精准的保育措施。国家重点研发计划(2016YFC0502900);;福建省科学技术厅民生科技专项(引导性项目)(2017Y6002);;福建省自然科学基金资助项目(2015J01122

The nutrient elements distribution in Casuarina equisetifolia seedlings under salt stress

利用盆栽试验,研究了盐胁迫下木麻黄幼树营养元素含量和分布规律。结果表明,不同施盐量处理的木麻黄幼树生长、营养元素含量及分布发生明显的变化。低浓度(5g/kg)NaCl处理促进了木麻黄的生长,而高浓度(20、25g/kg)则抑制木麻黄的生长。随着盐浓度的增加,木麻黄根中N、P、K,茎中N、P,小枝中N含量随之增加,并与盐浓度之间呈显著正相关,而茎和小枝中的K以及小枝中的Ca含量则与盐浓度之间呈显著负相关,说明盐胁迫下木麻黄营养元素的比例失调是导致盐害的主要原因之一。盐浓度对Mg元素影响较小。Based on pot experiments, the distribution of nutrient elements in Casuarina equisetifolia seedlings under salt stress were studied. The results showed that there were obviously effects on growth and distribution of nutrient elements in C. equisetifolia seedlings under salt stress. Low salt content (5g/kg) promoted the growth, while high salt content (20, 25g/kg) inhibited the growth. With the increase of salt content in soil, the contents of nutrient elements such as N, P, K in roots, N, P in stems and N in twigs increased, and the contents of K in stems and twigs and Ca in twigs decreased. It is suggested that the proportion maladjustment of nutrient elements is one of the causes resulted in salt damage. Salt content affected Mg content on a low level.福建省自然科学基金(D0120001)资

Survey and Evaluation of Heavy Metals, Nitrate and Nitrite Contamination in Vegetables in Xiamen's Market

为了解厦门市蔬菜中有害重金属、硝酸盐和亚硝酸盐的污染情况,于2004年8月至2005年12月从厦门市各超市、农贸市场、蔬菜批发市场和蔬菜产地上采集46个品种532份蔬菜样品,用国标法(GB/T5009.11-17-1996、GB/T5009.33-2003)分别分析蔬菜中的重金属、硝酸盐和亚硝酸盐的含量。结果表明,检测样品中Pb、Cd、As、Hg、硝酸盐和亚硝酸盐的平均值分别为0.0099、0.083、0.056、0.003、1090.3、0.59mg/kg;根据国家标准1-2,仅部分品种如菠菜、甘蓝、花菜、萝卜的铅超标,有潜在污染风险;大部分蔬菜中砷、汞、镉三种重金属的含量都较低,潜在的污染风险不大。硝酸盐污染程度严重的占36.5%;中、重度污染的占20.2%;轻度的占43.3%,硝酸盐含量依次为嫩茎叶菜类>根茎类>花菜类>瓜菜类>鲜豆菜>茄果类>水生蔬菜类,各样品间含量差别较大;而蔬菜中亚硝酸盐含量相对较低。The contents of heavy metal (Pd, Cd, As, Hg), nitrate and nitrite in vegetables from the markets and plant fields were investigated in Xiamen. The vegetable samples supplied seasonally were collected during August 2004 and December 2005. 532 samples in 46 groups of vegetables were analyzed according to GB/T5009.11-17-1996 and GB/T 5009.33-2003 to evaluate the status of the contamination. The results showed that the average contents are 0.0099, 0.083, 0.056, 0.003, 1090.3, 0.59 mg/kg for Pb, Cd, As, Hg, NO3-, NO2-, respectively. According to national standards[1-2], the samples of standard exceeding lead contents were found only in some limited vegetables. The potential trend of lead pollution was little. The contents of arsenic, mercury and cadmium in the most samples were very low, the pollution hazards of these three heavy metals were not serious. The prevalence of severe nitrate contamination was 36.5% in samples analyzed, the prevalence of heavy and medium nitrate contamination was 20.2%, the prevalence of mild contamination was 43.3%. The content of nitrate in vegetables was ranked in the following descent order, tender stem and leaf vegetables>root and stem vegetables>inflorescent vegetables>melon vegetables>beans>eggplant and fruit vegetables>water plant vegetables. However the contents in diferent species differed greatly. The nitrite in vegetables was comparably low

Study on the risk factors for intrauterine infection of hepatitis B virus among pregnant women

目的探讨厦门市孕妇乙型肝炎病毒(hepatitis B virus,HBV)感染情况和HBV宫内感染的影响因素。方法在1; 064名孕妇第一次到医院建立孕产妇保健卡时进行问卷调查并检测其血清乙肝标志物,根据检测结果选择外周血乙型肝炎表面抗原(hepatitis B; surface antigen,HBsAg)阳性的产妇179例随访至其分娩,并采集其分娩时的脐血和胎盘组织。对脐血进行HBsAg和HBV; DNA的检测,对胎盘组织进行HBsAg的检测。采用chi~2检验和非条件Logistic回归分析相关因素与HBV宫内感染的关系。结果被调查的1; 064名孕妇中,HBsAg阳性的孕妇共179名,感染率为16.8%。对179名HBsAg阳性的孕妇的脐血进行检测发现,发生HBV宫内感染的孕妇共; 34名,感染率为19.0%。孕妇HBV宫内感染的危险因素为胎盘HBsAg阳性、孕妇血清乙型肝炎E抗原(hepatitis B e; antigen,HBeAg)阳性、孕前超重,OR分别为5.123(95% CI:1.422 ~ 18.413)、4.619(95%; CI:1.225 ~ 17.534)、 3.343(95% CI:1.233 ~; 9.092)。结论对于HBsAg和HBeAg双阳性的超重/肥胖孕妇,其新生儿可能发生HBV宫内感染,应加强自身防护措施,合理规避HBV宫内感染风; 险。Objective To investigate the prevalence of hepatitis B virus (HBV); infection in pregnant women and the risk factors for Intrauterine; infection of HBV among pregnant women in Xiamen. Methods Total 1 064; pregnant women were included in this study. A questionnaire was; performed to collect the general information of the subjects and HBV; infection status was tested on the first day when they came to the; hospital. Then HBsAg positive pregnant women were selected and followed; until delivery. We collected their cord blood and placenta tissue when; they delivery. HBsAg and HBV DNA in cord blood and HBsAg in placental; tissue were detected. Chi-square test and the non-conditional Logistic; regression analysis were conducted to investigate the correlation; between HBV intrauterine infection and risk factors. Results 179 out of; the 1 064 pregnant women were HBsAg positive. The infection rate was; 16.8%. 34 out of 179 HBsAg positive pregnant women were found; intrauterine transmission with a rate of 19.0%. The risk factors for the; occurrence of HBV intrauterine transmission were placenta HBsAg positive; (OR = 5.123, 95% CI:1.422-18.413),mothers with hepatitis B e; antigen(HBeAg) positive (OR = 4.619, 95% CI:1.225-17.534) and; overweight(OR = 3.343, 95% CI:1.233-9.092), respectively. Conclusions; Placenta HBsAg positive,mothers with HBeAg positive and overweight are; independent risk factors for the occurrence of HBV intrauterine; transmission.国家自然科学基金; 厦门市科技计划项

Effects of Cadmium and Its Subcellular Distribution on the Photosynthesis of Cauliflower

研究Cd在花椰菜的残留分布及其对光合作用的影响,设置0、5、15、30mg·L-14个Cd浓度的水培试验,测定生长期间叶片的叶绿素含量,光合作用参数,Cd的亚细胞分布,化学形态分布和器官(根、茎、叶、花球)分布。结果表明,Cd处理两周后,叶片叶绿素a和b含量明显比对照降低,气孔导度、净光合速率和蒸腾速率亦显著降低,胞间CO2浓度变化不大。不同浓度Cd处理的叶片中Cd在亚细胞中的分布主要以细胞质组分为主(70.19%~74.40%),其次为细胞壁组分(22.56%~27.12%),叶绿体和线粒体组分仅占0.90%~2.49%。叶片中Cd的化学形态分布比率大小顺序为氯化钠提取态>去离子水提取态>醋酸提取态>乙醇提取态>盐酸提取态>残渣态。在不同器官分布上,Cd5mg·L-1处理下,花球Cd累积最少(14.50mg·kg-1),其次是茎叶(78.30~87.30mg·kg-1),而根的累积达857.90mg.kg-1。The effects of cadmium(Cd)(5,15,30 mg·L-1)on chlorophyll levels and photosynthesis of cauliflower(Brassica oleracea L.var.botrytis)were studied under different concentrations of Cd using hydroponics system.Meanwhile,the differential centrifugation techniques and sequential extraction methods were used to determine the subcellular distribution and chemical form of Cd in leaves.Cd contents in different parts of plant were also measured after harvest.The results showed that,after different contents of Cd were supplied for two weeks,except intercellular CO2 concentration(Ci),the levels of chlorophyll a and b,net photosynthesis rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)all decreased significantly compared with the control.For the leaves,Cd was mainly distributed in the fractions of cytoplasm(70.19%-74.40%)and cell wall(22.56%-27.12%),and less distributed in the fractions of mitochondrion and chloroplast(0.90%-2.49%).The sequential extraction results of Cd in leaves indicated that NaCl extractable Cd was predominated(33%-39%),and the distribution of six Cd forms in leaves was as following:F NaCl > FH2O> FHAC> FAlcohol> FHCl> FResidue.Most of Cd absorbed by plant was accumulated in roots,little of them was transported to the aboveground portions.The order of Cd accumulation in different plant parts was:roots>stems,leaves>flowers.厦门市科技计划项目(3502Z20071098

Green Synthesis of Potassium Diformate

以甲酸和氢氧化钾为原料,不外加任何溶剂一步合成二甲酸钾。通过单因素实验,考察了原料配比、反应温度和反应时间对产品质量与收率的影响。采用正交实验进一步优化了合成工艺,确定了最佳工艺条件:n(HCOOH)/n(KOH)=2.2、反应温度70℃、反应时间30 m in,在该条件下,二甲酸钾产品收率达98%以上。所得产品经红外光谱分析及熔点测定,其结果与文献报道一致。反应过程绿色化,有很好的原子经济性。Potassium diformate was synthesized without solvent,using formic acid and potassium hydroxide as raw materials.The optimal conditions for the reaction were:molar ratio of HCOOH to KOH 2.2∶1,reaction temperature 70 ℃ and reaction time 30 min.Yield of the process was over 98% under the optimal conditions.The infrared spectrum and melting range of the product were in good agreement with those reported in literature.The technology developed in this study could be an environmentally benign synthesis route with efficient atom economy

贵金属纳米颗粒及其催化剂的生物还原制备技术

利用自行筛选的具有强还原能力的菌株制备得到银纳米颗粒,所得颗粒的粒径基本在10 nm以下,主要集中在2~8nm。将生物还原过程进一步引入催化剂制备过程,得到负载型银催化剂和负载型钯催化剂,并将催化剂分别应用于1,2-丙二醇空气氧化合成丙酮醛、CO氧化生产CO2以及蒽醌加氢反应中

诱骗受体DcR3的基因构建、表达及特异性鉴定

目的:构建适于原核表达的人诱骗受体DcR3基因,并进行重组蛋白的表达纯化及特异性鉴定。方法:查得人DcR3cDNA全序列,将其分段设计引物,通过重叠PCR获得DcR3基因。构建pET-22b(+)/DcR3表达载体,转化大肠杆菌Rosseta-gami,IPTG诱导表达,Ni柱纯化。采用ELISA进行特异性鉴定。结果:通过重叠PCR获得了编码正确氨基酸序列的目的基因。目的蛋白以包涵体的形式表达,表达量占菌体总蛋白的30%以上。纯化后,蛋白纯度达95%以上。ELISA结果表明所纯化的蛋白可与抗DcR3抗体发生特异结合。结论:诱骗受体DcR3基因的成功构建、表达及纯化,为进一步的功能研究奠定了基础

Measurement of the Static Optoelectronic Characteristics of InGaAs/InP Avalanche Photodiode

建立了雪崩二极管的静态光电特性的自动测试系统。利用该系统对光敏面的直径为500μm的台面型InGaAs/InP雪崩光电二极管(APDs)进行测试。测试结果表明,该APD器件在90%击穿电压下的暗电流为151nA,在直径500μm的光敏面上其光响应均匀性良好。提出一种测量雪崩二极管倍增因子的方法,只需利用普通的测量电流-电压的测试仪器,就可以获得开始倍增时的光电流,从而得到APD的倍增因子。通过该方法得到的InGaAs/InPAPD器件最大倍增因子的典型值在10～100量级。A measurement system is set up which could measure static optoelectronic characteristics of avalanche photodiodes (APDs). By using this system, the mesa-structure InP/InGaAs APDs is measured. The results show that the APDs have a relatively low dark current (～150 nA at 90% of breakdown) and a uniform photoresponse profile of about 500 μm diameter. A method of getting APDs's multiplication gain is also proposed. Through getting the photocurrent at the point where multiplication is beginning, the multiplication gain can be obtained by the simple current-voltage equipment. For InP/InGaAs APDs, the typical maximum multiplication gain measured by this method is about 10～100