Studies of melanopsin in the mudskipper Boleophthalmus pectinirostris

大弹涂鱼（Boleophthalmuspectinirostris）是生活于潮间带淤泥滩涂的一种两栖鱼类。课题组前期研究表明，雌性大弹涂鱼生殖群体在繁殖季节出现半月周期的产卵现象，血清中的性类固醇激素、褪黑素、间脑中的褪黑素受体亚型基因mtnr1a1.4和卵巢中的褪黑素受体亚型基因mtnr1a1.7也具有明显的半月周期变化节律，并且半月周期峰值出现的时间与产卵时间基本一致。为了进一步了解大弹涂鱼黑视蛋白与褪黑素以及半月周期产卵习性的关系，本文采用分子生物学等方法克隆了大弹涂鱼四种黑视蛋白基因亚型（opn4m1、opn4m3、opn4x1、opn4x2），研究了其在不同组织、日周期、半月周期和不...The burrow-dwelling species of the mudskipper Boleophthalmus pectinirostris is widely distributed in the marine intertidal regions. In our previous study, during the spawning season, the female mudskipper stock exhibits a semilunar spawning pattern, variations of plasma steroid hormones, melatonin, melatonin recepter genes of mtnr1a1.4 in the diencephalon and mtnr1a1.7 in the ovary, were synchroni...学位：理学硕士院系专业：海洋与地球学院_海洋生物学学号：2232014115136

Design and Implementation of Xinjiang Grain Comprehensive Information Management System

当前，粮食信息资源是国家重要的战略资源，充分开发利用粮食信息资源是国家信息化的重要工作。如何实现粮食信息资源建设、管理和应用的一体化，整合应用、优化配置，建立信息共享互联互通的信息管理支撑体系及核心业务管理体系，推进业务协同和一站式服务平台建设,成为全国粮食系统信息化重点关注点。目前，全国粮食系统信息化管理水平处于起步阶段，技术含量不高，系统功能单一，资源整合性差，没有实现组织结构的重组和业务流程的再造。新疆粮食综合信息管理系统的建立，实现了新疆粮食信息数据的统一整合，业务流程的规范，是实现粮食信息化战略目标的一项基础性工作。 本课题的主要任务是根据新疆某部门粮食业务信息化的发展需求设计并实...At present, grain information resources are the important strategic resources, fully developing and utilizing of grain information resources is an important work for the national informatization. How to implement the grain information resources construction, and the integration of management and application , integrate application, optimize configuration, set up information management supporting s...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201223037

Analysis on Legislation of Electronic Evidence in China

我国在电子证据方面的法律缺失以及立法性文件的混乱，一直困扰着实务界。根据现代法治理念，法官不得借口法律有缺陷就拒绝适用、拒绝裁判。因此，实务界在操作中出现了在定性和证据力等问题上判断的不统一现象是可想而知的。21世纪是信息的社会，而信息社会是全球性的，以美国等主要的信息产业大国为代表的发达国家在立法中适时、明确地规定了电子证据的合法地位。我国在电子证据立法上的空白，必将导致我国在日益频繁的国际交往中的不利处境。因此，加紧电子证据的立法是当务之急。本文考虑到现今学术界已经对电子证据的定位问题进行了热烈的讨论，所以本文更多从比较法的角度考察我国立法并提出建议，同时也对电子证据在实际操作中遇到的问题...Electronic evidence is the outcome of the digital information technology. It is necessary to establish the legal value of electronic evidence by the promotion of economic in China and the development of evidence law. However, when the electronic evidence was brought to this complicated world, vacancy of legislation on electronic evidence and chaos of other judicial and government documents in Chin...学位：法学硕士院系专业：法学院法律系_诉讼法学学号：20030808

应用ARIMA模型预测厦门市2017年乙肝月发病率

目的分析厦门市乙型肝炎发病的流行病学特征,建立ARIMA模型并预测2017年厦门市乙肝月发病率,为厦门市乙肝防治提供理论依据。方法对2005-2016年厦门市乙肝报告发病数据进行统计描述,通过ARIMA模型预测2017年的乙肝月发病情况。结果厦门市2005-2016年乙肝报告年均发病率为45.70/10万,乙肝年发病率呈逐渐下降趋势;男性乙肝发病高于女性;各年龄组乙肝发病水平存在差异;15岁以下儿童乙肝发病率显著低于其他高年龄组的发病水平。建立模型ARIMA（1,1,1）×（0,1,0）12,模型的拟合效果较好,2017年厦门市乙肝月发病率预测值区间为[0.92/10万,4.76/10万]。结论厦门市2005-2016年乙肝发病总体呈现下降趋势,经预测2017年厦门市乙肝发病率与前几年相比波动不大,有轻微下降趋势,可用ARIMA模型对乙肝的短期发病情况进行预测

闽东南沿海城市高层住区布局与室外热环境优化——以厦门市为例

城市气候恶化的客观现实与国家新型城镇化转型战略为城市住区建设提出应对城市气候问题、优化室外热环境的艰巨任务。以厦门市高层住区为例,通过数值模拟与统计分析方法,探讨住区布局与室外热环境之间的作用机理,为住区设计形态生成提供必要依据。结果表明,通过布局优化改善室外热环境的思路具可行性;多指标均值综合得分评价体系可作为室外热环境分析的有效工具;建设用地方位、组团规模、建筑排布、绿地布局等均可对室外热环境产生重要影响。国家自然科学基金资助项目(51408516);;\n厦门市科技计划项目(XJK2017-1-11);;\n厦门大学“双一流”学科群支持项目(SAS2018-01);;\n福建省自然科学基金项目(2019J01007

Development and Application of a Novel Neutralization Test for Echovirus 25

目的:建立一种新型的快速、高通量的埃可病毒25型(ECHO25)中和抗体检测方法,并初步评价其在ECHO25中和抗体筛选和血清流行病学调查中的应用价值。方法:应用免疫荧光方法筛选ECHO25高亲和性抗体并将其作为检测单抗,结合酶联免疫斑点检测技术(ELISPOT)建立ECHO25中和抗体检测方法;使用不同效价的血清评价该方法的准确性;采用所建立的中和方法对ECHO25单克隆抗体、临床血清样品进行检测。结果:建立了快速检测ECHO25中和抗体的Nt-ELISPOT方法,以ECHO25单克隆抗体5B9作为检测抗体;相比经典的中和实验方法 Nt-CPE,该方法可显著缩短检测时间(从5~7 d缩短至1 d以内),检测结果具有较好的一致性;采用所建立的Nt-ELISPOT方法首次筛选获得3株对ECHO25具有较好中和能力的单克隆抗体;临床血清样品检测结果显示厦门地区可能存在ECHO25的流行。结论:该方法可以应用于中和抗体筛选和血清学的临床辅助诊断,为ECHO25的防治研究提供支持。Objective: To establish a rapid and high-throughput neutralization test for echovirus 25(ECHO25),and evaluate its application in neutralizing antibody screening and seroepidemiological surveys. Methods: Immuno-fluorescence assay was applied to screen a high affinity antibody, which was used as the detection antibody forECHO25, and a rapid neutralization test was established based on enzyme- linked immunospot assay(Nt-ELISPOT). The accuracy of this method was evaluated by detecting serum samples with different titer. Monoclonalantibodies against ECHO25 and clinical serum samples were detected via the established neutralization test. Results: A rapid method to detect neutralizing antibody against ECHO25 was established and an anti-ECHO25 anti-body, 5B9, was used as the detection antibody. The detection period could be shortened significantly comparedwith the classical neutralization test(Nt- CPE)(from five to seven days to less than one day), and the Nt-ELISPOT had good consistency with the Nt- CPE. Meanwhile, three neutralizing antibodies for ECHO25 werescreened firstly by this method. The detection results of clinical serum samples showed that infection of ECHO25 might be popular in Xiamen. Conclusion: This method can be used in neutralizing antibody screening and seroepi-demiological surveys, and it may provide support for the control of ECHO25.国家自然科学基金(81371817,81401669

Quantification of Methylation at CMV Promoter of pEGFP-C3 Vector by Gradient Touch-Down PCR Sequencing

目的改进亚硫酸氢钠测序法,并在CMV基因启动子甲基化检测中进行验证。方法提取PEgfP-C3质粒重组人肝癌细胞株HEPg2 dnA,亚硫酸氢钠化学修饰,针对修饰后质粒基因CMV启动子序列设计特异引物并结合梯度降落PCr扩增,T-A载体克隆、测序,目标区域甲基化定量。结果 PEgfP-C3质粒基因约600bP的CMV启动子区甲基化水平可以精确定量,检测结果与标准品一致,重复测量结果稳定。结论改进后亚硫酸氢钠测序法能明显减少非特异性扩增,提高PCr效率,更适于基因甲基化状态的检测。Objective To improve the sodium bisulfite sequencing method and validate it in quantification of methylation in CMV promoter.Methods DNA was extracted from recombinant HepG2 hepatoma cell line with plasmid pEGFP-C3,and chemically modified with sodium bisulfite,the gene-specific primers were designed according to the modified CMV promoter sequence and conducted PCR amplification with gradient touch-down PCR,then the DNA methylation level in the target areas was quantitated after T-A cloning and sequencing.Results The DNA methylation level of the 600 bp CMV promoter within pEGFPC3 plasmid could be quantified accurately,and be consistent with that of the methylated DNA standards,repeated measurements indicated stable quantification result with this method.Conclusion The improved sodium bisulfite sequencing method can reduce non-specific amplification significantly and improve PCR efficiency,therefore has more potential for quantitative detection of gene methylation status.国家自然科学基金资助项目(20907047);中央级公益性科研院所基本科研业务专项(2008KYYW05

Flexible decapyrrylcorannulene hosts

球形笼状的富勒烯是上个世纪末最重要的科学发现之一，但对富勒烯的精确几何结构的认识却困难重重，原因是单晶中球形分子的取向往往是无序的，需通过笼外衍生或通过八乙基金属卟啉-富勒烯超分子主客体组装来固定富勒烯的取向，然后利用常用的单晶衍射分析技术来精确表征富勒烯的几何结构。然而许多富勒烯新结构因无法与卟啉主体形成高质量的单晶至今仍无法利用X射线衍射技术进行结构分析，直接制约了对富勒烯形成机理及结构-性能关系的深入认识。功能团簇材料创新研究群体的谢素原、张前炎课题组另辟蹊径地从曲面结构的十氯碗烯C20Cl10出发，合成了十吡咯取代的碗烯分子C20(C4H4N)10。结构分析表明该分子的结构特征是碗烯的碳框架与十个吡咯基团通过单键相连。实验还证明，用甲基去取代吡咯3, 4-位置的氢并不利于富勒烯与碗烯衍生物形成有序的超分子组装体，理论研究进一步诠释了十个吡咯‘手指’的集体贡献比单个碗烯‘手掌’更大的原因。该研究工作是功能团簇材料创新研究群体长期积累，并由校内外十多位研究人员共同努力完成。徐云彦（2014级硕士生）、田寒蕊（2014级博士生）和李姝慧（2016年进站博士后）为该论文共同第一作者。【Abstract】The assembly of spherical fullerenes, or buckyballs, into single crystals for crystallographic identification often suffers from disordered arrangement. Here we show a chiral configuration of decapyrrylcorannulene that has a concave ‘palm’ of corannulene and ten flexible electron-rich pyrryl group ‘fingers’ to mimic the smart molecular ‘hands’ for self-adaptably cradling various buckyballs in a (+)hand-ball-hand(−) mode. As exemplified by crystallographic identification of 15 buckyball structures representing pristine, exohedral, endohedral, dimeric and hetero-derivatization, the pyrryl groups twist with varying dihedral angles to adjust the interaction between decapyrrylcorannulene and fullerene. The self-adaptable electron-rich pyrryl groups, susceptible to methylation, are theoretically revealed to contribute more than the bowl-shaped palm of the corannulene in holding buckyball structures. The generality of the present decapyrrylcorannulene host with flexible pyrryl groups facilitates the visualization of numerous unknown/unsolved fullerenes by crystallography and the assembly of the otherwise close-packed spherical fullerenes into two-dimensional layered structures by intercalation.This research was supported by the National Natural Science Foundation of China (21771152, 21721001, 21827801, 51572231, 51572254, 21571151, 2170010228), the 973 Program of China (2014CB845601 and 2015CB932301), the China Postdoctoral Science Foundation (2016M602067), the National Key Research and Development Program of China (2017YFA0402800), and the Fundamental Research Funds for the Central Uni- versities (20720170028, 20720160084). Q.Y.Z. is particularly grateful to 21771152, 2015CB932301, 20720170028, 20720160084; S.F.Y. is particularly grateful to 51572254 and 2017YFA0402800; S.Y.X. is particularly grateful to 21721001 and 51572231; L.S.Z. is particularly grateful to 21827801; S.L.D. is particularly grateful to 21571151; S.H.L. is particularly grateful to 2170010228 and 2016M602067. 研究工作得到国家自然科学基金（21771152、21721001、21827801、51572231、51572254, 21571151、2170010228）、科技部973计划（2014CB845601、2015CB932301）和重点研发计划（2017YFA0402800）、国家博士后科学基金、中央高校基本科研业务费等的资助