Organci-Inorganic Hybrid Silica Monolith Based Covalently Bonded Aptamer Affinity Chromatography Column for Thrombin Detection

Aptamers, generally selected by the SELEX(systematic evolution of ligands by exponential) approach, are short(100nt )DNA-or RNA-based oligonucleotides.Aptamers, generally selected by the SELEX(systematic evolution of ligands by exponential) approach, are short(100nt )DNA-or RNA-based oligonucleotides

Organci-Inorganic Hybrid Silica Monolith Based Covalently Bonded Aptamer Affinity Chromatography Column for Thrombin Detection

Organci-Inorganic Hybrid Silica Monolith Based Covalently Bonded Aptamer Affinity Chromatography Column for Thrombin Detectio

多种蛋白质肽段同时印迹聚合物微球材料及其制备和应用

本发明涉及一种可用于多种目标蛋白质选择性识别的分子印迹微球材料及其制备过程。所述的分子印迹微球材料是利用多种目标蛋白质各自所对应的特异性肽段为模板分子，以高分子聚合物为基质，采用聚合物相反转的方法制备得到。并且将该微球材料用于标准目标蛋白的选择性识别中

磁性氧化石墨烯纳米银复合材料及制备和应用

本发明涉及一种糖肽特异性富集的磁性氧化石墨烯纳米银复合材料制备。根据氧化石墨烯和氨基四氧化三铁之间共价反应，制备磁性氧化石墨烯。磁性氧化石墨烯作为载体，聚乙烯亚胺作为还原剂和稳定剂，在其表面原位生成和固定纳米银颗粒。磁性氧化石墨烯纳米银复合材料作为亲水固定相用于糖肽特异性富集。在该方法中，磁性氧化石墨烯纳米银复合材料成功用于糖肽亲水富集。该发明在蛋白质组学有较好的应用前景和实用价值

Preparing a metal-ion chelated immobilized enzyme reactor based on the polyacrylamide monolith grafted with polyethylenimine for a facile regeneration and high throughput tryptic digestion in proteomics

Initially, a poly (glycidyl methacrylate-co-acrylamide-co-methylenebisacrylamide) monolith was prepared in the 100 mu m i.d. capillary, and then was grafted with polyethylenimine (Mw, similar to 25,000) for adsorbing Cu(2+), followed by chelating trypsin. As a result, efficient digestion for BSA (100 ng/mu L) was completed within 50 s via such immobilized enzyme reactor (IMER); yielding 47% sequence coverage by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Compared with the conventional method for preparing the metal-ion chelated IMER, the regeneration of such IMER can be achieved facilely by the respective 30 min desorption and re-adsorption of trypsin, and 51% sequence coverage was obtained for 50 s BSA digestion after regeneration. BSA down to femtomole was also efficiently digested by the prepared regenerable IMER. Meanwhile, after the consecutive digestion of myoglobin and BSA, there was not any mutual interference for both during MALDI-TOF MS identification, indicating the low nonspecific adsorption of such regenerable IMER. To test the applicability of regenerable IMER for complex sample profiling, proteins (150 ng) extracted from Escherichia coli were digested within 80 s by the regenerable IMER and further analyzed by nanoreversed phase liquid chromatography-electrospray ionization-mass spectrometry successfully, showing its practicability for the high throughput analysis of complex samples

一种失忆性贝毒分子印迹整体柱及其应用

本发明涉及一种失忆性贝毒分子印迹整体柱的制备方法和应用。将适当配比的模板分子、功能单体、交联剂、致孔剂（十二醇、1,4-丁二醇）溶解于二甲基亚砜中，超声混合5分钟、静置20分钟，加入引发剂（偶氮二异丁腈）占单体总量的0.5～1%，充氮除氧5分钟，将此溶液充满石英毛细管，封端，60～70℃恒温聚合12～14小时，将毛细管连接到高效液相色谱输液泵上，清洗除去模板分子和致孔剂，最后得到具有良好分离效果的毛细管整体柱。本发明制备的分子印迹整体柱具有良好的选择识别性和小的传质阻力，作为高效液相色谱微柱，可实现对贝类样品中软骨藻酸的分离、富集与检测