Study on Vibration Characteristics of Wheeled Tractor Power Output Assembly

通过对大功率轮式拖拉机动力输出总成系统的激励分析、振动模态分析以及对系统固有特性的研究,分析了轴不平行度几何因素对总成系统的振动影响,研究了轴承游隙、轴承预紧力等物理因素对总成系统的固有特性影响。通过将轴承游隙、轴承预紧力控制在一个合理范围内,降低了系统的固有频率,减小了动力输出总成系统的振动。Based on the excitation analysis and vibration modal analysis of a large power wheeled tractor power output assembly system and study of the system intrinsic characteristics,the influence of shaft nonparallelism on assembly system vibration is analyzed. In addition,the influence of physical factors such as bearing clearance and bearing preload on inherent characteristics of the assembly system is studied. By controlling the bearing clearance and bearing preload within a reasonable range,the natural frequency of the system is reduced,and the vibration of the assembly system is also reduced.工信部智能制造专项“新型轮式拖拉机智能制造新模式应用”（豫洛工业制造[2016]07744

Application of Accelerated Solvent Extraction Technique for Analysis of Active Components in Traditional Chinese Medicinal Herbs

以两种药材为研究实例,对加速溶剂萃取法(ASE)在中药材有效成分提取研究中的应用进行了简要介绍。采用正交试验法考察了提取丹参中丹酚酸B的提取条件(萃取温度、静态萃取时间、萃取溶剂以及料液比),确定了较好的实验条件。比较了ASE、水蒸气蒸馏法、超声波提取法及索氏提取法对木香挥发油的提取效果,结果表明ASE对木香挥发油的提取效果最好。The application of accelerated solvent extraction(ASE) technique for the Analysis of active components in traditional Chinese medicinal herbs was introduced by using two kinds of herbs as examples.The conditions including extraction temperature,static extraction time,the ratio of material to solvent and solvent of ASE for extraction of salvianolic acid B in Salvia miltiorrhiza were optimized by orthogonal experiments,and the optimal conditions were obtained.Different extraction methods(ASE,steam distillation,ultrasonic wave and Soxhlet extraction) were used to extract volatile oil in Aucklandia lappa Decne.Results of the comparative experiments indicated that ASE was the most effective method in this case.All the results from these studies demonstrate that ASE is indeed a powerful tool in the preparation of herbal extracts for downstream chromatographic analysis.青岛“2004将才计划”(04-3-JJ-11);; 共建生物医药研发测试中心(LS-05-KJZX-76)资

仙女堡水电站桩基承载力有限元分析研究

为了论证桩基作为仙女堡水电站地基加固手段的理论的可行性,有必要计算桩基础承载力.以3#控河闸为例采用ANSYS建立了有限元模型,将三种水位与三种桩长组合成3种工况.计算结果表明三种水位工况下的变位及应力变化规律一致,且校核工况下变位及应力水平最高,其次为正常蓄水位,最次之为设计洪水位.天然工况下的地基沉降均超出了规范允许值,闸底板下游右侧铅直应力超过地基承载力,从理论上得出地基加固必要性.根据工程实际,对深厚覆盖层引起闸室的沉降、应力进行深入的研究,分析资料详细,可为类似工程提供借鉴经验

Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints

To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints.The samples were extracted using ASE for 10 min under 100 ℃ and 9.65 MPa,and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively.Based on this method,the HPLC fingerprints of Radix gentianae were established.Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value,three compounds in water phase were identified as gentiopicroside,asafetida acid and loganic acid.There is no report of the compounds in chloroform phase.The content of gentiopicroside and loganic acid in samples of different groups were determined,separately.The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint.The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90,however,the chloroform phase fingerprint congruence coefficients were within 0.62-0.99.This method can be used for determination of potent component in Radix gentianae and its quality control.Radix gentianae from different producing areas have the largest diversities,and the diversities embodied in the content of chloroform phase compounds.青岛“2004将才计划”(04-3-JJ-11);; 国家海洋局青年基金资助项目(2005602);; 崂山区政府区校共建生物医药研发测试中心资助(LS-05-KJZX-76)

Determination of Gentiopicroside and Loganic Acid in Radix Gentianae by MEKC and MEEKC Mode

目的建立胶束电动毛细管色谱(MEKC)和微乳液毛细管电动色谱(MEEKC)分析龙胆药材中龙胆苦苷和马钱子苷酸含量的方法。方法采用加速溶剂萃取法(ASE)对龙胆药材进行提取,萃取温度:100℃,压力:9.65MPa,萃取时间:10min。采用未涂层熔融石英毛细管(内径75μm,有效长度50cm)。分别考察了两种分离模式下电泳介质的构成和电泳过程中的各操作参数对样品分离过程的影响,优化了MEKC和MEEKC的分析条件,在各自对应的缓冲液体系下,MEKC和MEEKC分离电压分别为30和22kV,柱温均为25℃,检测波长均为238nm。结果在选定的工作条件下,龙胆苦苷和马钱子苷酸与其他组分达到了基线分离,两种成分的浓度与其响应信号值之间具有较好的线性相关性,加标回收率在96.3%～105.1%之间,检测限均低于10mg·L-1,对6处不同产地的龙胆药材进行了分析,并对测定结果进行了t检验,结果表明,两种模式下,测定结果之间不存在显著性差异,而不同产地的龙胆药材的龙胆苦苷和马钱子苷酸含量之间存在较大差异。结论本方法简便,准确,快速,重现性较好,可用于龙胆药材有效成分的含量测定和质量控制。OBJECTIVE To develop MEKC and MEEKC modes for the determination of gentiopicroside and loganic acid in extracts of Radix Gentianae. METHODS The analyte was extracted from Radix Gentianae samples by accelerated solvent extraction, and the extraction conditions were optimized. Separation and determination were carried out on a bared fused silica capillary(50 cm×75 μm) with corresponding buffer. The run voltage of MEKC and MEEKC were 30kV and 22 kV respectively. Detection wavelength of DAD was at 238 nm and column temperature was 25 ℃. RESULTS The developed ASE-HPCE method was simple and reliable for the determination of gentiopicroside and loganic acid in Radix Gentianae samples with a broad linear dynamic range, a recovery range of 96.3%～105.16%,and the detection limit was below 10 mg·L-1. The contents of gentiopicroside and loganic acid in six samples from different regions were determined by the developed method in two modes. T-test value indicated that the determined contents of gentiopicroside and loganic acid by MEKC and MEEKC were consistent. CONCLUSION The method is simple,accurate,rapid and with good reproducibility.It can be used to determine active components in Radix Gentianae.国家自然科学基金重点项目(20235020);; 青岛“2004将才计划”(04-3-JJ-11);; 崂山区校区共建生物医药研发测试中心资助(LS-05-KJZX-76

Effects of Moxibustion on Gastric Stress Ulcer Rats' Apoptosis Protein Phosphorylation

目的:研究艾灸对应激性胃溃疡大鼠胃黏膜细胞凋亡蛋白质磷酸化的影响,探讨艾灸促进胃黏膜损伤修复的信号转导机制。方法:将大鼠随机分为正常组、模型组、胃经穴组和对照点组,采用束缚冷应激法制作应激性胃溃疡大鼠模型,肉眼观察大鼠胃黏膜损伤程度,APOPTOSIS MICrOArrAy SlIdES芯片检测胃黏膜细胞凋亡蛋白质磷酸化水平。结果:与模型组比较,胃经穴组和对照点组大鼠胃黏膜损伤指数值均显著降低(P<0.05);与对照点组比较,胃经穴组大鼠胃黏膜损伤指数显著降低(P<0.05);APOPTOSIS MICrOArrAy SlIdES芯片检测结果显示:与模型组比较,胃经穴组大鼠胃黏膜细胞10种蛋白质磷酸化水平上调,其中bCl-Xl、MCl-1、bCl-2、IAPS 4种蛋白磷酸化水平差异有统计学意义(P<0.01,P<0.05);18种蛋白质磷酸化水平下调,其中Tnf、fAS、APAf-1、CASPASE-3、CASPASE-9、bAX 6种蛋白质磷酸化水平差异有统计学意义(P<0.01,P<0.05)。结论:艾灸可促进胃黏膜的损伤修复,调节多种凋亡相关信号蛋白质的磷酸化水平,并且存在一定的经脉脏腑相关性。Objective: To study the effects of moxibustion on apoptosis protein phosphorylation in rats with gastric stress ulcer and to explore the signal transduction mechanisms promoting gastric mucosal injury and repairing moxibustion.Methods: The rats were randomly divided into normal group,model group,stomach meridian group and the control point groups and each group had 10 rats.The stress ulcer rat model was established by using restraint cold stress method.We observed the rat gastric mucosa injury degree and Apoptosis Microarray Slides microarray was used to observe the gastric apoptosis protein phosphorylation levels.Results: Compared with the model group,the gastric mucosa injury index values of control point and meridian groups were significantly lower( P < 0.05).Compared with the control point group,a significant reduction in rat gastric mucosa injury index was in meridian group( P < 0.05).Apoptosis Microarray Slides microarray results showed that 10 kinds of protein phosphorylation levels increased,among which Bcl- XL,Mcl- 1,Bcl-2 and IAPs protein phosphorylation levels were statistically significant( P < 0.01,P < 0.05) and 18 kinds of protein phosphorylation levels decreased,among which TNF,Fas,Apaf- 1,Caspase- 3,Caspase- 9 and Bax protein phosphorylation levels were statistically significant( P < 0.01,P < 0.05).Conclusion: Moxibustion can promote gastric mucosal injury and repair and regulate phosphorylation of several proteins related to apoptotic signals and there is a certain correlation between the meridians organs.国家自然科学基金项目(30960484;81260556

大孔吸附树脂分离纯化龙胆药材中龙胆苦苷和马钱子苷酸的研究

目的:建立利用大孔吸附树脂对龙胆药材中龙胆苦苷和马钱子苷酸进行富集和分离纯化的方法。方法:采用加速溶剂萃取法对龙胆药材中两种有效成分进行高效提取,比较了D301,AB-8,D101,XDA-1四种大孔树脂对龙胆苦苷和马钱子苷酸的吸附性能,最终确定采用D301型大孔对脂对二者进行富集吸附,对其工艺参数进行优化,全程采用高效液相色谱进行目标化合物浓度检测。结果:化优后的工艺参数为:上样浓度:0.2 g/mL,最大上样量:0.25 g龙胆药材/g树脂,最佳静态吸附时间:8 h,采用8%和55%的乙醇溶液对龙胆苦苷和马钱子苷酸分别进行洗脱;龙胆苦苷和马钱子苷酸分别富集在8%和55%的乙醇洗脱液中,洗脱液浓缩后冷冻干燥,可得到纯度分别为74.3%和80.9%的粗产物,龙胆苦苷和马钱子苷酸的回收率分别为70.11%和67.82%。结论:此法效率较高,操作简便,即可用于实验室制备少量的难以购置的标准品,也可进行放大研究,用于工业生产。国家自然科学基金重点项目(20235020);; 青岛“2004将才计划”(04-3-JJ-11);; 共建生物医药研发测试中心(LS-05-KJZX-76)资

RNA 干扰 mPGES-1 基因对 K562/A 细胞增殖及凋亡的影响

【目的】探讨RNA干扰膜结合型前列腺素E2合酶1（mPGES-1）对阿霉素耐药的人红白血病细胞株K562/A细胞增殖、凋亡及耐药性的影响及其可能的机制。【方法】通过RNA干扰技术抑制K562/A细胞中mPGES-1表达。分组：①未处理组（K562/A），②干扰后的阴性对照组（K562/A-NC），③干扰组（K562/A-KD），④干扰后加入外源性PGE2组（K562/A-KD+PGE2）；CCK-8法检测细胞活力；流式细胞术检测细胞凋亡；ELISA法检测PGE2浓度；Western blot检测蛋白水平。【结果】RNA干扰可明显下调K562/A细胞mPGES-1表达，抑制PGE2合成（<0.0001）。RNA干扰后，K562/A细胞增殖受抑，凋亡增加，对各化疗药物敏感性均有不同程度的增强（<0.05），同时β-catenin、MDR1的表达量减少（<0.01）。外源性PGE2可逆转RNA干扰对K562/A增殖、凋亡水平、药物敏感性的影响（<0.05），同时β-catenin、MDR1的表达上调（<0.01）；β-catenin抑制剂XAV939可浓度依赖性抑制β-catenin、MDR1蛋白的表达（<0.05）。【结论】RNA干扰mPGES-1基因可抑制K562/A细胞增殖、诱导细胞凋亡、增强细胞对化疗的敏感性，其机制与减少PGE2的合成进而下调β-catenin、MDR1的表达有关。Wnt/β-catenin通路可能参与了mPGES-1/PGE2对MDR1的调控

Anti-resonance features of destructive quantum interference in single-molecule thiophene junctions achieved by electrochemical gating

基于单个有机分子来构筑电子器件为电子器件微型化提供潜在技术方案。本研究发展了可集成电化学门控的单分子电子器件测试芯片技术和科学仪器方法，在实验和理论两个层面对具有相消量子干涉效应的噻吩衍生物分子器件的电输运过程进行了电化学调控研究，从而首次在室温下实现了对单分子电子器件中量子干涉效应的反共振现象的直接观测和调控，为制备基于量子干涉效应的新型分子材料和器件提供了全新的设计思路和策略。该研究充分展示了电化学调控技术在信息材料和器件领域的重要应用潜力，也体现了我校固体表面物理化学国家重点实验室在电化学研究和科学仪器研发领域的技术积累，以及面向科学前沿开展交叉学科探索的研究特色。 该研究工作是在洪文晶教授、上海电力大学陈文博教授、英国兰卡斯特大学Colin Lambert教授指导下完成的。化学化工学院博士生白杰和李晓慧为论文的共同第一作者，刘俊扬副研究员、师佳副教授、研究生唐永翔、刘帅、黄晓娟、谭志冰和萨本栋微纳研究院的杨杨副教授等也参与了研究工作。田中群教授和毛秉伟教授为该工作提供了重要指导。【Abstract】Controlling the electrical conductance and in particular the occurrence of quantum interference in single-molecule junctions through gating effects, has potential for the realization of high-performance functional molecular devices. In this work, we used an electrochemically-gated, mechanically-controllable break junction technique to tune the electronic behaviour of thiophene-based molecular junctions that show destructive quantum interference (DQI) features. By varying the voltage applied to the electrochemical gate at room temperature, we reached a conductance minimum that provides direct evidence of charge transport controlled by an anti-resonance arising from DQI. Our molecular system enables conductance tuning close to two orders of magnitude within the non-faradaic potential region, which is significantly higher than that achieved with molecules not showing DQI. Our experimental results, interpreted using quantum transport theory, demonstrate that electrochemical gating is a promising strategy for obtaining improved in-situ control over the electrical performance of interference-based molecular devices.This research was supported by the National Key R&D Program of China (2017YFA0204902), National Natural Science Foundation of China (21722305, 21673195, 21503179, 21703188), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Natural Science Foundation of Shanghai (17ZR1447100), Science and Technology Commission of Shanghai Municipality (14DZ2261000), China Postdoctoral Science Foundation (2017M622060) for funding work in Xiamen. It was also supported by EU Horizon 2020 project QuIET under grant agreement no. 767187EC FP7 ITN ‘MOLESCO’ project no. 606728 and UK EPSRC grants EP/N017188/1 and EP/M014452/1 and Leverhulme Trust (Leverhulme Early Career Fellowships no. ECF-2017-186 and ECF-2018-375) for funding instrumentation used in Lancaster. It was also supported by Hungarian and Czech Academies of Sciences (P2015-107) and Hungarian Research Foundation (OTKA 112034) for funding instrumentation used in Hungary. The authors thank Z.-Q. Tian and B.-W. Mao, Xiamen University, for useful discussions. 该工作获得科技部国家重点研发计划课题（2017YFA0204902），国家自然科学基金委优秀青年科学基金等项目（21722305、21673195、21703188、21503179）以及中国博士后科学基金（2017M622060）等项目的资助，也得到了固体表面物理化学国家重点实验室、能源材料化学协同创新中心的支持

厌恶性条件反射与脑中c-fos的表达

Ｆｏｓ免疫组织化学法作为一种神经功能活动形态定位法，已在大鼠厌恶性条件反射的神经机制的研究中得到应用。无论是在味觉厌恶性条件反射的研究中，还是在听觉刺激与电击相结合的厌恶性条件反射的研究中，都证明了条件反射建立后，条件刺激能诱导出与非条件刺激相似的ｃ－ｆｏｓ表达的分布，这提示条件反射学习沟通了条件刺激和非条件刺激这两种不同刺激在大脑中的传导通路