84 research outputs found

    SCANNING ELECTRON MICROSCOPICAL OBSERVATION ON ADULT NEOBENEDENIA MELLENI(MONOGENEA,CAPSALIDAE)

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    报道寄生于福建海水养殖鱼类高体的玫氏新本尼登虫 (单殖吸虫纲 ,分室科 )的扫描电镜观察。虫体体表无棘 ,前吸器和后吸器盘状。副甲片、前钩和后钩位于鞘内Neobenedenia melleni(MacCallum,1927) Yamaguti,1963,a capsalid monogenean parasitising the skin of marine cultured fish,Seriola dumerili(Carangidae) was studied by scanning electron microscope (SEM).The body surface of N.melleni lacks spines.Both the anterior attachment organs and haptor are disc shaped,the accessory sclerites,anterior hamuli and posterior hamuli are sheathed in tegument.Accessory sclerites possess sharp distal tips.Anterior hamuli and posterior hamuli possess curved tip,and marginal hooklets are arranged radially around haptor.ThisworkwassupportedbytheFujianProvincialNaturalScienceFoundation (B 991 0 0 0 5

    党的巡视、超额在职消费及其渠道效应——基于中共十八大反腐建设的研究

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    十八大以来,党中央大力推进反腐败斗争与党风廉政建设。随着巡视制度被以党内法规的形式确立为党内监督制度,巡视工作也被赋予更多的使命与职责,目前已完成对国家机关、国有企事业单位等组织的巡视,这为我们开展巡视制度的相关研究提供了重要的现实基础。本文以被巡视国有企业的下属上市公司为检验样本,采用PSM-DID的分析方法,考察党的巡视对企业腐败行为的影响。研究发现:(1)党的巡视能够显著抑制国企高管的超额在职消费行为;(2)党的巡视对超额在职消费的抑制作用在垄断程度较高的行业更为显著;(3)渠道效应研究表明,党的巡视通过加强媒体的报道、约束地方的保护主义,从而抑制国企高管的超额在职消费行为。分位数回归及安慰剂检验结果进一步验证了结果的稳健性。本文的研究为检验反腐政策的实施效果提供了微观角度的经验证据,这为今后进一步完善党的巡视制度,加强党的建设提供了有力支撑,同时也为他国学习中国经验提供了一定借鉴。国家自然科学基金面上项目“审计与腐败治理:理论、路径与效果”(71702052)广东省2017-2018年度会计科研课题(2017A09)广州市哲学社会科学发展“十三五”规划2018年度课题(2018GZYB113

    鼠腹腔中弓形虫的增殖发育及S.D.对其的影响

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    对鼠腹腔中弓形虫速殖子的增殖发育的方式、过程及 S.D.对其的影响作了详细的考察 ,结果表明弓形虫的无性生殖方式有内二裂殖、纵二裂殖、裂殖生殖等 ,虫体以鼠腹腔内巨噬细胞的胞壁作为假包囊壁在其中增殖发育 ,经过 72 h的增殖生长 ,虫体成熟后突破假包囊逸出 ,S.D.可部分抑制速殖子增殖

    A New Species of the Genus Nanhaipotamon (Decapoda:Potamid)

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    记述了采自福建省武平县的淡水蟹类南海属Nanhaipotamon一新种武平南海溪蟹N.wupingensissp.nov.本新种头胸甲显著隆起,雄性第1腹肢末部宽大,内侧呈半圆形扩张,外末角呈指状突出,末缘中部明显隆突.蟹体并殖吸虫囊蚴感染率27.3%(3/11).Nanhaipotamon wupingensis sp. nov(Fig.1~8)Holotype:♂,carapace length 22 mm,breadth 27.5 mm;allotype: ♀,length 20 mm,breadth 25 mm;paratypes: 3♂2♀,collected from Xiaba village of Wuping county,Fujian (116°2′N.,24°53′E.);October,1998;195~260 metres above sea level;breadth of the stream 0.5~1.5 metres,pH 6.0.All the type specimens are deposited in Fujian Research Institute of Parasite Disease,Fuzhou,Fujian Province.17 crabs were examined,3 of them infected with the metacercaria of Paragonimus westermani and Euparagonimus cenocopiosus.Carapace distinctly convex, surface smooth, cervical groove shallow.The end of the first pleopod of male,exceeding the suture between 4~5th sternum,Subteminal segment 3.0 times as long as the terminal segment. Terminal part widely,anteroborde mountain peaklike.On side distal end half roundshaped and strongly expanded abdominalinner.The subdistal segment of the second pleopod of male about 2.7 times as long as the distal segment. The abdominal of male triangularshaped,the sixth abdominal segment about 2.3 times as broad as long

    A New Genus Record of Phyllobothriidae from Marine Fish Dasyatis akajei in China

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    报道检获于厦门海域赤魟(Dasyatis akajei)肠道中的四叶目叶槽科蔷薇属叶枕蔷薇线虫Rhodobothriumpulvinatum Linton 1889,经鉴定比较,为我国鱼类绦虫属的新记录。本次共剖检赤魟46尾,阳性2尾,感染率为4.3%,感染强度为5~7条/尾,并对所获虫体进行了描述。Rhodobothrium pulvinatum, one species of Phyllobothriidae (Cestoda: Tetraphyllidea) from marine fish Dasyatis akajei in Xiamen, Fujian, China was reported. The genus Rhodobothrium Linton, 1889 was erected for R. pulvinatum from the intestine of Dasyatis kuhli. which was new genus record in China, Dasyatis akajei was also a new record of host. 46 of Dasyatis akajei were examined, 2 of which were infected with cestode, the infective rate was 4. 3%, the infective intensity of the cestode was 5-7, the description of the worm is provided in the present paper.厦门大学校级自选课题资助(2002

    ANALYSES ON LDH ISOENZYME AND PROTEIN FROM BOTH GUANGDONG STRAIN AND FUJIAN STRAIN OF ECHINOCHASMUS FUJIANENSIS

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    目的 探讨福建与广东两地福建棘隙吸虫生化特点。方法 用不连续聚丙烯酰胺凝胶电泳及薄层等电聚焦电泳等进行比较分析虫体乳酸脱氢酶 (LDH)同工酶和蛋白质。结果 福建与广东两地虫株都有 5种LDH同工酶 ,其中LDH1、LDH4 和LDH5的迁移率相同 ;蛋白质电泳都分离出 9条区带 ,其中有 7条带的等电点相同。但在LDH2 和LDH3 的迁移率 ,蛋白质区带的相对含量 ,同工酶的总活力与比活力 ,以及A和B亚基的含量等存在差异。结论 LDH同工酶与蛋白质的某些差异 ,可归因为基因型相似条件下所出现两地虫株生化遗传上差异Aim\ Inguired into the biochemical characteristrics of Echinochasmus fujianensis found in Fujian and Guangdong provinces. Methods\ Compared and analysed two strains by LDH isoenzyme eletrophoresis, discontinuous PAGE and thinlayer isoelectric equilibrium PAGE.Results\ Two strains both had five LDH isoenzyme, among them, LDH\-1、LDH\-4 and LDH\-5 had the same mobilities. There were nine bands in protein electrophoresis. Seven bands had the same isoelectric point.But there were differences in the mobilities of LDH\-2 and LDH\-3,the comparative amount of protein bands, total and specific activity of isoenzyme and the amount of A and B subunit. Conclusion Under the similar condition of genotypes, the differences of the biochemical inheritance can be contributed to the differences of LDH isoenzyme and protein.福建省科技计划基金资助项目!(No.98-Z-156);; 福建省“百千万人才”人选培养资金资助项

    SURVEY OF MIXED INFECTIONS OF FISH-BORNE TREMATODES IN PINYUAN COUNTY AND DNA(RAPD)ANALYSIS

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    目的 调查广东省平远县棘隙吸虫及其他鱼源性吸虫混合感染情况。方法 对鱼类宿主取鱼鳃和鱼肉等组织 ,镜检囊蚴 ;用水洗过筛沉淀法粪检虫卵 ,部分虫卵阳性动物解剖检查。采用DNA -RAPD技术 ,比较福建棘隙吸虫广东株与福建株。结果 查出华支睾吸虫 (Clonorchissinensis)、东方次睾吸虫 (Metorchisorientalis)、福建棘隙吸虫 (Echinochasmusfujianensis)、日本棘隙吸虫 (E japonicus)、抱茎棘隙吸虫 (E perfoliatus)和钩棘单睾吸虫 (Haplorchispumilio) 6种。第二中间宿主为麦穗鱼等 11种 ,感染率 48 3% (182 / 377) ,有 92 4%的阳性鱼混合感染 2种以上吸虫囊蚴。保虫宿主狗、猫感染率 79 2 % (19/ 2 4) ,每只阳性动物均有 2种以上吸虫混合感染。 15个引物在福建棘隙吸虫广东株与福建株两者共获DNA片段 185个 ,共享度 (F) =0 984,遗传距离 (D) =0 0 16。结论 当地为人兽共患的多种鱼源性吸虫混合感染区。形态学观察和分子生物学检测结果相印证 ,福建棘隙吸虫广东株与福建棘隙吸虫福建株为同一虫种。Aim To seek the possible existence of mixed infections of Echinochasmus fujianensis and other fish-borne tremtodes in Pinyuan county,Guangdong.Methods Metacercariae of trematodes were detected from gills,muscles and some other tissues of freshwater fish and examintd under microscopy Sedimentation pocedures were used to observe parasite ova in stool Adult worms were obtained from definitive animal hosts upon dissection Experimental animal infection were performed from metacercariae of E fujianensis strains in Fujian and Guangdong,and it's DNA analysis Random amplified Polymorphic DNA(RAPD).Results Six species of trematodes were found,including Clonorchis sinensis,Metorchis orientalis,E fujanensis, E japonicus,E perfoliatus and Haplorchis pumilio Eleven species of freshwater fish comprising Pseudorasbora parve were identified as the second intermediate hosts,with an average positive rate of 48 33%(182/377) The overall mixed infection animal were contracted with 2 or more species Dogs and cats were detected to be reservoir hosts,the total infection rate being 79 2%(19/24).92 4% of infecded fish were contracted with 2 or 6 speies of the metacercariae of trematodes 185 DNA fragments were obtained by 15 primers,F=0 894 and D=0 016 between E fujianensis Guandong and Fujian strain Conclusion There are six species of fish-borne trematodes which may cause infections in human and animal in the regions E fujianensis Guangdong stain and Fujian strain are the same species福建省科技计划基金!资助项目 (No 98-Z - 1 56);; 福建省“百千万人才”人选培养资

    Electric-field-induced selective catalysis of single-molecule reaction

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    随着单分子电学检测技术的迅速发展,分子电子学的研究不再局限于分子电子学器件的构筑及其电学性质的测量,而且扩展到单分子尺度化学反应过程的探索。然而目前相关的研究仍然局限于理论计算方面,在单分子尺度上实时监测和调控化学反应的活性和选择性是化学领域的长期目标和挑战。针对这一挑战,洪文晶教授课题组与程俊教授课题组合作,自主研发了精密科学仪器,将单个有机分子定向连接在两个末端尺寸为原子级的电极之间,解决了化学反应中分子取向控制的问题.理论计算结果证实了定向电场可以有效地稳定化学反应的过渡态,从而降低反应能垒。该研究工作在化学化工学院洪文晶教授、程俊教授、能源材料化学协同创新中心(iChEM)刘俊扬副研究员的共同指导下完成,由硕士研究生黄晓艳、iChEM博士研究生唐淳、博士研究生李洁琼以及兰州大学的陈力川博士作为共同第一作者,化学化工学院师佳副教授、陈招斌高级工程师、夏海平教授和田中群教授,萨本栋微纳研究院杨扬副教授、环境与生态学院白敏冬教授以及兰州大学张浩力教授参与了研究工作的讨论并给予指导,博士后乐家波、博士研究生郑珏婷、张佩(已毕业)、李瑞豪、李晓慧也参与了研究工作。Oriented external electric fields (OEEFs) offer a unique chance to tune catalytic selectivity by orienting the alignment of the electric field along the axis of the activated bond for a specific chemical reaction; however, they remain a key experimental challenge. Here, we experimentally and theoretically investigated the OEEF-induced selective catalysis in a two-step cascade reaction of the Diels-Alder addition followed by an aromatization process. Characterized by the mechanically controllable break junction (MCBJ) technique in the nanogap and confirmed by nuclear magnetic resonance (NMR) in bottles, OEEFs are found to selectively catalyze the aromatization reaction by one order of magnitude owing to the alignment of the electric field on the reaction axis. Meanwhile, the Diels-Alder reaction remained unchanged since its reaction axis is orthogonal to the electric fields. This orientation-selective catalytic effect of OEEFs reveals that chemical reactions can be selectively manipulated through the elegant alignment between the electric fields and the reaction axis.This work was supported by the National Key R&D Program of China (2017YFA0204902), the National Natural Science Foundation of China (21722305, 21703188, 21673195, 21621091, 51733004, 51525303, and 91745103), the China Postdoctoral Science Foundation (2017M622060), and the Young Thousand Talents Project of China. 该工作得到国家自然科学基金委(21722305、21703188、21673195、51733004、51525303、91745103),国家重点研发计划课题(2017YFA0204902),中国博士后面上基金(2017M622060)的资助,以及固体表面物理化学国家重点实验室、醇醚酯化工清洁生产国家工程实验室、能源材料化学协同创新中心的支持

    A list of fish cestodes reported from China

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    This paper lists 107 fish cestode species belonging to two subclasses, 10 orders, 25 families and 54 genera reported from Chinese freshwater and marine fishes. These species are tabulated together with their fish hosts, distribution in China and Chinese waters, and the publications in which they are recorded
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