Experimental study on reperfusion of intraocular lens

作者简介: 祁明信, 男, 1945 年7 月 出生, 教授、主任医师、博士研究生 导师, 主要从事白内障的基础与临 床研究。联系电话: 0591-83570887; E-mail:qihuang@netease. com 通讯作者: 黄秀榕,E-mail:[email protected][中文文摘]目的开展晶状体再灌注的离体和动物实验研究,并对再灌注人工晶状体技术进行评价。方法采用新鲜离体幼兔眼、离体猪眼、新西兰白兔眼,应用自行研制的人工晶状体材料,进行以下实验:(1)体外固化实验;(2)晶状体前囊膜微型撕囊及其稳固性实验;(3)经微型前囊膜开口超声乳化吸出晶状体内容物实验;(4)活的新西兰白兔眼内人工晶状体再灌注实验。结果(1)按硅酮聚合物与固化剂50:1的比例可获得柔软、弹性好、固化时间短(完全固化时间为60min)的注入材料;(2)晶状体前囊膜1.8～2.0mm的连续环形撕囊口具有较好的稳定性,可经该微型开口吸出晶状体内容物并灌注材料;(3)超声能量18%、流量25mL·min-1、负压120mmHg(1kPa=7.5mmHg)为晶状体内容物经微型前囊膜开口吸出的最佳条件;(4)注入灌注材料后可形成由晶状体囊膜包裹的、置换原晶状体皮质和核的、新的再灌注人工晶状体。结论采用再灌注人工晶状体的方法可进行新型人工晶状体再灌注,可为治疗白内障和老视提供参考。[英文文摘]Objective To carry out the experimental study on reperfusion of intraocular lens(IOLs) in vitro or in animal,and to assess the technique of IOLs reperfusion.Methods The following experiments were performed by using self-developed materials in fresh rabbit eyes and pig eyes in vitro,as well as in eyes of alive New-Zea-land rabbits:(1)Solidification study of self-developed material in vitro;(2)Continuous circular capsulorhexis(CCC) in anterior capsule of lens and its stability;(3)Draw of lens contents via phaco through mini-CCC;(4)IOLs ref illing in the eyes of alive New-Zea land rabbits. Results(1) Thematerialwhich was soft, springy and short-term solidification(full solidification time was 60 minutes) were obtained in certain proportion of geland solidified agent(50:1) in vitro; ( 2)The CCC in anterior capsule of lens with 1.8-2.0 mm diameter had very good stability. The lens contents were drawn and the materialwere refilled through themini-CCC; (3) The best conditions of drawing out lens contents through m ini-CCC were phaco energy 18% , flow 25 mL·min- 1, and negative pressure 120 mmH g (1kPa=7.5 mmHg);(4) The new refilled IOLs, which were wrapped by capsule of lens and were replaced original cortex and nucleus of lens, were obtained after thematerial refillied. Conc lusion. New IOLs are refilled through this method, which can prov ide reference for the treatment of cataract and presbyopia.福建省科技三项费用;教育厅重点资助项目基金资助(编号:K98041

Yang Changji and the Establishment of China's Ethics Disciplinary System

徐朝旭,厦门大学哲学系教授、博士生导师,哲学博士;杨海秀,厦门大学哲学系博士研究生,广西医科大学人文社会科学学院讲师。【中文摘要】新中国成立前我国现代伦理学学科体系的建构已经走过约半个世纪的历程。以民国建立为分期的标志,这一历史进程大体上分为两个阶段:第一阶段(1895—1912年)是现代伦理学学科体系建构的萌芽时期;第二阶段(1912—1949年)是现代伦理学学科体系初步形成的时期。杨昌济作为民国早期学者,为我国伦理思想的转型与现代伦理学学科的创建做出了多方面的贡献。在理论体系的建构、学科教育的开拓、研究范式和方法的转换等方面,杨昌济都进行了卓有成效的工作;尤其在西方伦理学史学科的建设方面,他的贡献具有奠基性的意义。 【Abstract】Before the founding of the People's Republic of China, the modern ethics disciplinary system had been evolving for about half a century. With the establishment of the Republic of China as the demarcation line, the historical process of the system falls roughly into two phases – the first phase (1895-1912) being the brewing period for the system and the second phase (1912-1949) being its formative period. As a scholar in the early period of the Republic of China,Yang Changji is credited with significant contributions to the transformation of Chinese ethics thought and the creation of a modern disciplinary system for ethics. He was highly accomplished in the development of the theoretical system for ethics,in the expansion of education in ethics as an academic subject, and in the research of the transformation of paradigms and methodology. He also made groundbreaking contributions to the development of the disciplinary system of Western ethics in China.国家社科基金重点项目“儒学、儒教与儒家宗法集体主义”(13AZJ001

Application of DNA functionalized gold nanoparticles

金纳米颗粒拥有独特的光学和电学性质,被广泛地应用于分析检测、催化和生物医学等领域.通过改变尺寸和形状能够可控地调节金纳米颗粒的等离子体共振吸收峰,进而改变金纳米颗粒的光学和电学特性.而在金纳米表面修饰dnA,使金纳米颗粒具有dnA的特异性识别能力和可寻址能力,则能够有效地扩展金纳米颗粒在纳米组装、环境监测、疾病诊断、药物运输和纳米颗粒合成方面的应用.本文系统总结了不同大小金纳米颗粒的合成方法,探讨如何在金纳米颗粒上快速修饰dnA,研究了影响金纳米颗粒表面dnA的修饰密度、功能及稳定性的因素,并描述了dnA功能化的金纳米颗粒在检测、组装、载药和纳米颗粒合成中的应用.本文将进一步加深人们对dnA功能化金纳米颗粒的制备、性质及应用的了解,为dnA功能化金纳米颗粒的研究提供指导.Gold nanoparticles have been widely applied to different natural sciences due to its unique optical and electrical properties.It is easy to control the size and shape of gold nanoparticles to adjust the absorption peak of gold nanoparticles and change the optical and electrical properties.When gold nanoparticles are functionalized with DNA, they are endowed with the recognition ability and addressability of DNA, and it further widens the applications of gold nanoparticles in nanoparticle assembly, environmental monitoring, disease diagnosis, drug delivery, and nanoparticle synthesis.First, this review systematically summarized the synthesis of gold nanoparticles with different sizes.Second, it discussed how to rapidly functionalize gold nanoparticles with DNA.Moreover, it studied the factors that affect the density, function, and stability of DNA on gold nanoparticle surface.Finally, it described the applications of gold nanoparticles in detection, nanoparticle assembly, drug delivery, and nanoparticle synthesis.This review would promote understanding of the preparations, properties, and applications of DNA functionalized gold nanoparticles, and would provide guidance for the research of DNA functionalized gold nanoparticles.国家重点基础研究发展计划(2010CB732402;2013CB933703); 国家自然科学基金(91313302;21205100;21275122); 国家杰出青年科学基金(21325522)的资

幼丹顶鹤感染血孢子虫的PCR鉴定

2006～2008年,北京动物园繁殖的17只1.5～3.5月龄丹顶鹤幼鹤出现厌食、精神倦怠、呼吸困难等症状。采用PCR方法鉴定病原,使用特异性引物对线粒体细胞色素b、细胞色素氧化酶Ⅰ、18S rRNA及线粒体部分非编码区基因进行扩增、克隆、测序,结合GenBank中已知疟原虫序列,经DNAMAN软件处理分析,并对Cytb序列用软件MEGA 4.0建NJ树。序列分析表明丹顶鹤血孢子虫靶序列与鸟疟原虫序列相似性达到98%以上,进化关系分析表明与残疟原虫各株属同一个种群,证明该病病原为残疟原虫。但该血孢子虫与残疟原虫已报道各株形态有一定差异,可能为寄生于丹顶鹤的一个新基因型