Mirk/Dyrk1b在上皮性卵巢癌组织中的表达及意义

目的:探讨Mirk/Dryk1b(Minibrain-related kinase/Dual specificity tyrosine-phosphorylation-regulated kinase1B)在卵巢组织中的表达及其临床意义。方法:利用免疫组化检测Mirk/Dyrk1b在30例上皮性卵巢癌、20例上皮性卵巢囊腺瘤、10例正常卵巢组织中的表达。结果:Dyrk1b在上皮性卵巢癌中的表达明显高于上皮性卵巢囊腺瘤及正常卵巢组织(P0.05)。结论:Dyrk1b在上皮性卵巢癌中高表达,提示其可能参与了肿瘤的发生和发展,并有望成为临床早期诊断的肿瘤标志物和新的卵巢癌治疗的靶基因

聚乳酸基热固性聚氨酯及其形状记忆性能

通过聚乳酸二元醇和聚乳酸-聚己内酯共聚物二元醇与六亚甲基二异氰酸酯(HDI)三聚体交联反应合成了一系列生物基热固性聚氨酯(Bio-PUs)。利用傅里叶红外(FTIR)、差示扫描量热分析(DSC)、热失重分析(TGA)、万能拉伸机和细胞毒性等测试方法对获得的聚乳酸基聚氨酯进行了表征。结果表明,与聚乳酸二元醇相比,聚乳酸-聚己内酯共聚物二元醇降低了生物基热固性聚氨酯的玻璃化温度(Tg),提高了热固性聚氨酯的热稳定性;且聚乳酸-聚己内酯型聚氨酯的力学性能和形状记忆性能更为优异。其中,聚乳酸-聚己内酯共聚物二元醇分子量为3 000时得到的热固性聚氨酯(Bio-PU2-3000)的杨氏模量为277.7 MPa,伸长率为230%;聚乳酸-聚己内酯共聚物二元醇分子量为1 000得到的热固性聚氨酯(Bio-PU2-1000)在人体体温下的形变回复时间仅为93 s。另外,通过显微镜观察到细胞在含聚乳酸基热固性聚氨酯的培养液中生长状态良好,表明制备得到的生物基聚氨酯无细胞毒性

一种螺旋片导流式相分离装置

本发明提供了一种螺旋片导流式相分离装置，包括分离管，所述分离管根据作用区域依次分：掺混段，内部设置有静态混合器，所述静态混合器用于将输入的液体进行充分混合；旋流段，内部安装有旋流器；分离段，经过所述旋流段的液体在此段能够在离心力的作用下充分分离成内外两个旋流场；排出段，包括位于所述分离管端头上的重相排管，和一端沿所述分离段的轴心线插入所述旋流段内另一端穿过所述分离管侧壁的轻相排管。本发明具有装置尺寸小、重量轻、分离效率高等优点。本发明适用于工业生产过程中需要对密度不同的两相混合流体进行分离的多种场合，从

The involvement of FoxO in cell survival and chemosensitivity mediated by Mirk/Dyrk1B in ovarian cancer

Minibrain-related kinase (Mirk) is a serine/threonine kinase which is also known as the dual specificity tyrosine-phosphorylation-regulated kinase 1B (Dyrk1B). It is known that Dyrk1A, the closest family member to Mirk/Dyrk1B can mediate cellular localization of mammalian forkhead subclass O (FoxO1), a transcription factor, although the effect of Mirk/Dyrk1B on Foxes factors remains to be defined. In this study, we showed that Mirk/Dyrk1B protein was overexpressed in 5 of 8 ovarian cancer cell lines and negatively correlated with the protein level of FoxO factors (FoxO1+FoxO3A). Knockdown of Mirk by small interfering RNA (siRNA) resulted in cell apoptosis and sensitized cells to cisplatin accompanied by nuclear translocation of FoxO1 and/or FoxO3A as well as increased Bim, TRADD, cleaved caspase-3 and PARP. Furthermore, combined siRNAs of Mirk with FoxO1 and/or FoxO3A led to fewer apoptotic cells and cisplatin sensitivity compared to Mirk siRNA alone, suggesting that FoxO is involved in Mirk-mediated cell survival and chemosensitivity of ovarian cancer. Taken together, Mirk/Dyrk1B plays an important role in ovarian cancer cell survival through modulating FoxO translocation and may be a novel therapeutic target for ovarian cancer.National Natural Science Foundation of China [81172457]; Natural Science Foundation of Fujian Province, P.R. China [2010J01236

面向电子封装装备制造的若干关键技术研究及应用

隨著電子信息產業的高速發展,高性能電子封裝裝備成為眾多半導體器件制造企業的重大需求。我國自主精密電子封裝裝備的研發有待于在設計理論與方法上取得突破。針對高性能封裝裝備在大行程、高速、高加速度運動與高精度定位的綜合性能指標方面的苛刻要求,深入開展其核心關鍵技術的創新設計與理論研究,提出一種解耦式高速XY并聯運動平臺,分析平臺的工作空間及黎曼度量評價方法;針對高速輕載執行機構在其運動及定位過程中振動慣性能快速衰減難題,提出基于慣性能時空分布最優的結構優化和運動規劃新方法,可有效減少執行機構末端和運動末段的能量聚積,實現高速運動條件下的快速精密定位;面向高性能封裝裝備對控制系統的高響應速度和精密定位精度需求,提出一種多核多任務控制器設計與驅控一體化的控制系統方案,可有效提高控制系統的實時性、可靠性和協調性;同時,開發高速精密封裝裝備閉環控制所需的宏微復合絕對光柵檢測裝置,實現高速運動過程執行機構位置信息的采集與反饋,保證高速運動過程的精密定位;同時研究鍵合工藝過程的劈刀運動軌跡及鍵合界面的沖擊力影響因素。綜合各項技術研究成果,成功開發出高性能引線鍵合機裝備。As the rapid development of electronic and communication industry, high performance electronic packaging equipment has been the great demand for most semiconductor manufacturing enterprises. The research and development of precision electronic packaging equipment needs obtaining the breakthrough on the design theory and methods. Considering the restrict requests on the motion of large stroke, high speed, high acceleration with high positioning accuracy, the innovation design and theory on the kennel technologies are intensively studied, and a decoupled parallel XY motion stage with high speed is proposed. Based on the workspace analysis and riemannian metric evaluation, the stage parameter optimization is performed. Due to the motion with a high speed and high acceleration, it is difficult to reduce rapidly the inertial energy and the vibration to the high-speed low-load mechanism. To tackle this problem, a novel structural optimization and motion planning method is proposed for precision positioning of high speed mechanism based on optimal spatial and temporal distribution of inertial energy. Considering the demands of high responding speed and positioning accuracy of the control system in packaging equipment, a control system scheme with a multi-kennel and multi-task controller and an integrated driving-controlling system are adopted, which can improve the performance of real time, reliability and coordination. In order to realize a close-loop position control for the high-speed movement, an novel linear optical encoder with absolute imaging position system is invented, which can provide the position information and feed back to the control system for the precision positioning. The capillary motion path of the bonding process and the impact force to the bonding interface are also studied. Based on these key technologies, the wire bonding machines with high performance are developed with success. © 2017 Journal of Mechanical Engineering

复合式油水分离系统

本发明公开了一种复合式油水分离系统，其包括依次连接的第一旋流管本体(10)、梯型管本体(30)和第二旋流管本体(20)，所述梯型管本体(30)包括平行设置的下水平管(31)、上水平管(33)以及与所述下水平管(31)、上水平管(33)垂直连通的若干等间隔的垂直管(32)。利用本发明的分离系统进行分离的方法综合运用离心、重力、膨胀复合等多种原理，克服了采用单一原理的分离方法对于不同工况下处理效率偏低的缺点，提高了分离效率，改进了分离技术，减轻了分离器重量。本发明的分离系统适合于陆上油田和海上油田使用，有很好的工业应用前景