Studies on the Preparations, Characterizations of DicarboxylateCoordination Complexes and Reaction Properties

为了理解金属－有机配位聚合物形成过程、掌握配位聚合物目标分子形成的规律,本文主要研究了温度对邻苯二甲酸、顺丁烯二酸以及丁二酸配位模式和配合物最终结构的影响以及二胺配体和丁二酸根·四水合镍、钴、锌（II）配位聚合物的取代反应，以及这两个体系中的物种的分离、表征和反应性能的研究。主要结果总结如下：1、以刚性的邻苯二甲酸和顺丁烯二酸为桥联配体时：低温不利于其与金属的配位，羧酸处于游离状态或与金属形成单核、双核或多核配合物；高温较易与金属配位，桥联金属形成配位聚合物，但邻苯二甲酸因空间位阻的关系，有时亦可能仍形成单核配合物。当羧酸两端的羧基的夹角接近于90º时，配合物比较稳定，这与RHF6/...In order to understand the forming process of coodination polymers and get the general and precise principle for the solid structures of the target products, in this paper, we have studied temperature effect on the final structures of phthalate, maleate and succinate coordination complexes, and diamine substitution reactions of tetrahydrate succinate coordination polymers. The results are summari...学位：理学博士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：B20032504

巴西橡胶树若干品系叶切片的超微结构观察

本文对巴西橡胶的若干品系叶片进行了显微和超微结构的观察。实验结果表明：巴西橡胶不同品系间的叶片解剖结构存在明显差异：古农９６－２８，ＲＲＩＭ６００和ＩＡＮ８７３叶片维管束鞘细胞含有丰富的叶绿体，基粒片层较发达，且韧皮部薄壁细胞，木质部薄壁细胞和射线细胞也含有叶绿体，但没有典型的“花环型”结构；ＩＡＮ８７３叶片维管束鞘细胞里富含两种类型的光合膜、线粒体和发达的内质网等多种细胞器。而天任 ３１－４５等的叶片鞘细胞仅含少量叶绿体，其片层不发达，且无基粒

推荐一个基础有机化学新实验——微波辐射合成肉桂酸酯

推荐一个基础有机化学实验———微波辐射合成肉桂酸酯,即在常压条件下以肉桂酸和醇为原料,浓硫酸为催化剂,利用微波辐射技术快速合成肉桂酸甲酯、乙酯及丙酯,收率可达91%～95.2%

非诺贝特对小鼠急性局灶性脑缺血再灌注损伤的保护作用

目的研究非诺贝特(fenofibrate,Fen)对小鼠急性局灶性脑缺血再灌注损伤的保护作用及机制。方法线栓法制备小鼠大脑中动脉栓塞模型,缺血90 min后再灌注。非诺贝特(10,80 mg.kg-1)再灌注同时及再灌后2 h各灌胃给药1次。再灌注后24 h,测定小鼠神经功能缺失评分、脑梗死体积及脑水肿程度,实时逆转录多聚酶链反应(RT-PCR)法检测过氧化物酶体增殖物激活受体α(PPARα)mRNA的表达水平,生化法测定脑组织丙二醛(maiondialdehyde,MDA)含量及超氧化物歧化酶(superoxide dismutase,SOD)的活性,伊文思蓝(Evans blue,EB)法观察血脑屏障破坏程度;应用过氧化物酶体增殖物激活受体α拮抗剂MK886(10 mg.kg-1),观察过氧化物酶体增殖物激活受体α是否参与非诺贝特的脑保护作用。结果非诺贝特(80 mg.kg-1)可改善小鼠神经功能缺失,减小脑梗死体积,减轻脑水肿程度,减少脑缺血后脑内伊文思蓝的渗漏,上调脑损伤后脑内过氧化物酶体增殖物激活受体αmRNA的表达,减轻脑组织的脂质过氧化。MK886可拮抗非诺贝特的保护作用。结论非诺贝特可通过上调过氧化物酶体增殖物激活受体αmRNA表达、减轻脂质过氧化损伤而对小鼠急性局灶性脑缺血再灌注损伤发挥保护作用

Application of immunofluorescence to C_3/C_4 attribute identification of a tropical crop, Coffea arabica

用从烟草提纯的 1,5-二磷酸核酮糖 ( Ru BP)羧化酶制备兔抗 Ru BP羧化酶抗体 ,并以异硫氰酸盐荧光素 ( FITC)标记抗体 .采用直接免疫荧光法对典型 C3植物水稻、C4 植物甘蔗和小粒种咖啡等进行了 Ru BP羧化酶的组织化学定位 .结果表明 :C3和 C4 植物叶切片中 Ru BP羧化酶的分布明显不同 ,C3植物的特异荧光位于叶肉细胞 ,C4 植物的特异荧光绝大部分位于维管束鞘细胞 ;小粒种咖啡的特异荧光仅分布在叶肉细胞 .因此认为 ,小粒种咖啡应属 C3植物Rabbit antiserum raised purified ribulose 1,5 bisphosphate carboxylase (RuBPCase) from tobacco was used to locate RuBPCase in leaf blade transection of classical C 3, C 4 plants and Coffea arabica by direct immunofluorescence method. The antibody was labelled by fluoresecin isothiocyanate (FITC). It was discovered that in classical C 4 plant (sugarcane), the specific fluorescence was located almost exclusively in bundle sheath cell chloroplasts, while in C 3 plant (rice), the specific fluorescence was in mesophyllous cell chloroplasts, which proved the difference in RuBPCase location between classical C 4 and C 3 plants. The specific fluorescence of C.arabica was located only in mesophyllous cell chloroplasts, so it was concluded that C.arabica belongs to C 3 plant

Function of a methyl-accepting chemotaxis protein Tlp1 in Azorhizobium caulinodans ORS571

【目的】考察茎瘤固氮根瘤菌中趋化基因簇上游的受体蛋白Tlp1编码基因的突变表型,初步探究其功能机理。【方法】利用同源重组和三亲本接合转移的方法构建突变株,在TY培养基中测定生长情况,半固体平板法观察趋化圈,刚果红固体培养基观察胞外多糖和次生代谢产物的分泌,乙炔还原法测定菌株的固氮酶活性。【结果】与野生型菌株相比,tlp1突变株的生长速率没有影响。在以甘油为碳源的L3半固体平板上突变株的趋化圈变小,其回补菌株能部分回补趋化能力。突变株的胞外多糖分泌与野生型没有区别,但其次生代谢产物黑色素出现的时间比野生型稍早。在固氮酶活性测定中,发现突变株酶活性明显比野生型降低,回补菌株能够部分回补。【结论】茎..

PHYSIOLOGICAL RESPONSES of HEVEA BRASILIENSIS DURING THE CHILLING INJURY

在人工零上低温下,巴西橡胶树(HEVEAbrASIlIEnSIS)叶质膜透性随低温处理时间的延长而持续上升,抗冷品系的上升速率比不抗冷品系慢。呼吸强度和ATP含量均随处理时间的延长而持续下降,抗冷品系下降速率比不抗冷品系慢。叶绿体Mg-(++)—ATPASE活性也表现出明显抗性差异。可见,低温下叶组织的质膜透性、呼吸强度、ATP含量以及Mg-(++)—ATPASE活性的变化与品系抗冷性有关。低温下呼吸强度、ATP含量与质膜透性变化呈负相关,质膜透性的变化与供能有关。Under the reFrigerating conditions ( low temperatures of above oC ) , the permeability of plasma membrane in Hevea brasiliensis was rising steadily with the prolongation of low temperature treatment, and the rising rates of chilling resistant clones were slower than those of chilling-sensitive clones, The respiratory intensity and ATP content were dropping with the prolongation of treatment time, and the dropping rates of chilling resistant clones were slower than those of chilling sensitive clones.The resistant clones alones also displayed a noticeable change in the activities of chloroplasts Mg ++-ATPase, obviously diFFerent From those of the sensitive clones.It is thus seen that the changes of the permeability of plasma membrane, respiratory intensity, ATP content and Mg++-ATPase activity are related to the chilling resistance of clones.The respiratory intensity and ATP content under low temperature negatively related to .the change of plasma membrane permeability which has a bearing on the supply of energy

考虑结构柔性的行星轮系齿轮啮合特性分析与优化

以某AT变速器行星轮系为研究对象，利用有限元法分析了行星架、齿圈和壳体的动态特性。并结合模态实验，验证了壳体有限元模型的准确性。建立了AT行星轮系动力学分析模型并进行了接触斑点实验，通过仿真与实验接触斑点结果对比分析得出，小太阳轮-短行星轮、长行星轮-齿圈齿轮副的啮合斑点基本一致且存在明显的偏载现象。通过导入柔性行星架、齿圈和壳体有限元模型得到了完整的AT变速器动力学模型，重点分析了行星架、齿圈、壳体柔性对行星齿轮传递误差、啮合错位量等啮合特性参数的影响，结果表明，壳体柔性和齿圈柔性变形对齿轮啮合错位以及传递误差的影响最为显著，行星架柔性影响次之。通过提高支撑轴承刚度、轴承预紧力和齿轮参数优化的方法，改善了行星轮系齿轮啮合特性，优化了行星轮系齿轮强度以及AT变速器NVH性能

Synthesis of Acetanilide under Microwave Irradiation

报道了采用微波技术制备乙酰苯胺的方法,同时对影响收率诸因素进行考察,确定了最佳反应条件:微波辐射功率600W,苯胺与冰醋酸的摩尔比为1∶2.5,反应时间20min,乙酰苯胺的收率可达80.6%.与常规加热反应对比,发现采用微波辐射法缩短了反应时间,提高了反应速率和收率.The use of microwave irradiation has attracted much attention in organic synthesis in recent decades.We have employed this method for the synthesis of acetanilide,giving 80.6% yields in 20 minutes.Compared with traditional methods,this new method has the advantages of less agent consumption and higher yields in a short time