Study on the Synthesis and Application of Novel Biomaterials

新型生物材料的制备和应用研究 摘要 本论文共分五章，包括仿生智能生物材料、溶胶凝胶生物材料和纳米荧光探针的研究进展；新型环境敏感高分子的合成与应用；溶胶凝胶生物材料的合成与应用和荧光团杂化纳米二氧化硅微球的合成与应用；研究工作的结论与展望。 第一章为绪论，评述了生物材料（biomaterials）对现代生物工程和医学工程发展的重要意义及该领域中已取得的一些令人激动的研究成果，本章分为三部分。第一部分介绍了仿生智能材料—环境敏感性高分子的作用机理、合成方法、性质研究和生物学应用研究；第二部分对溶胶凝胶技术作了简介，并对近年来溶胶凝胶技术固定生物活性物质的研究进行了综述；第三部分评述了纳...Study on the Synthesis and Application of Novel Biomaterials Abstract This dissertation consists of five chapters, including the overview of intelligent biomaterials, sol-gel biomaterials and nanometer fluorescence probes; synthesis and application of novel environment sensitive polymers; synthesis and application of novel sol-gel biomaterial; synthesis and application of nanometer fluorescence ...学位：理学博士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：B19992500

双能谷效应对N型掺杂Si基Ge材料载流子晶格散射的影响

性能优越的Si基高效发光材料与器件的制备一直是Si基光电集成电路中最具挑战性的课题之一.Si基Ge材料不仅与成熟的硅工艺相兼容,而且具有准直接带特性,被认为是实现Si基激光器最有希望的材料.对Si基Ge材料N型掺杂的研究有利于提示出其直接带发光增强机理.本文研究了N型掺杂Si基Ge材料导带电子的晶格散射过程.N型掺杂Si基Ge材料具有独特的双能谷（Γ能谷与L能谷）结构,它将通过以下两方面的竞争关系提高直接带导带底电子的占有率:一方面,处于Γ能谷的导带电子通过谷间光学声子的散射方式散射到L能谷;另一方面,处于L能谷的导带电子通过谷内光学声子散射以及二次谷间光学声子散射或者直接通过谷间光学声子散射的方式跃迁到Γ能谷.当掺杂浓度界于1017cm-3到1019cm-3时,适当提高N型掺杂浓度有利于提高直接带Γ能谷导带底电子占有率,进而提高Si基Ge材料直接带发光效率.国家自然科学基金青年基金(批准号:61604041);;福建省自然科学基金青年基金(基金号:2016J05147);;福建省教育厅2017年高校杰出青年科研人才培育计划项目;;福建工程学院校科研启动基金(批准号:GY-Z14073)资助的课题~

海洋溢油生态损害的简易评估和综合评估方法

通过对海洋生态系统服务的讨论,结合生态学及经济学的评估方法,提出对不同规模溢油事故的区别性评估方法体系:简易评估和综合评估.中小型溢油选用公式化的简易评估法,大型溢油污染事故则建议从生物资源、海洋生境、环境容量、景观文化开展综合评估,并分别提出相应的评估方法.简易评估在美国佛罗里达公式的基础上,进行了适于我国国情的修改,该方法对信息量的要求小,简单易行,其推算的赔偿范围符合以往的索赔案例,可在今后的中小型溢油中推广实践并不断完善.综合评估中,对生物资源的评估,实现了非经济物种的价值化;生境价值评估采用在我国鲜有应用的生境等价分析;环境容量评估是我国海洋生态损害评估研究相对较多的内容,在价值化时结合已有的环境规划项目,可使其在相关索赔中更为合理.景观文化是在以往案例中很少被考虑的非使用价值,随着生态损害评估方法体系的不断完善,应在今后的实践中得以有效合理的赔偿

Analysis of Diterpene Quinones in Danshen(Salvia Miltiorrhiza Bunge) by HPLC-ESI/TOFMS

采用高效液相色谱-电喷雾飞行时间质谱(HPLC-ESI/TOFMS)联用技术,研究4种丹参酮的分子结构与裂解规律间的关系,并对丹参酮中的脂溶性成分进行鉴定。采用反相C18色谱柱,以甲醇-0.1%乙酸+5mmol.L-1乙酸铵溶液为流动相,二元等度洗脱。通过与电喷雾飞行时间质谱联用,获得丹参中脂溶性成分的精确相对分子质量和分子式,采用质谱碰撞诱导解离技术获得各化合物碎片的裂解信息,并对丹参中的16种丹参酮类化合物进行了初步鉴定。研究结果表明,高效液相色谱-电喷雾飞行时间质谱联用技术能够简便、快速、有效地鉴别丹参中的丹参酮类化合物。The diterpene quinone components in lipid soluble fractions of Danshen were studied by HPLC-ESI/TOFMS.The lipid solubles were extracted with a mixture of chloroform and methanol(1∶4,V/V).The extract was dried by a gentle stream of nitrogen,and the residue was diluted in methanol for analysis.HPLC was performed on a reversed-phase C18 column using isocratic elution with a mixture of methanol and water with the addition of 0.1% acetic acid and 5 mmol·L-1 ammonium acetate.Accurate molecular weights of individual tanshinones were determined through ESI-TOF/MS on-line detection.The molecular formula and TOFMS fragmentation behavior of each compound were obtained through off-line analysis under different collision induced dissociation(CID) energies.Combining literature information with the observed data,16 tanshinones in lipid-soluble fractions of Danshen were tentatively identified in a run.The method demonstrates high potential as a rapid and effective method for the preliminary structural screening and speciation of bioactive tanshinone components in Danshen or other herbal extracts.国家自然科学基金“面上项目”(20675021);; 青岛共建生物医药研发测试中心项目(LS-05-KJZX-76)资

合成气制低碳混合醇耐硫钼基催化剂的制备研究

本文考察不同制备方法和制备程序制得之MoS_x-K~+/SiO_2催化剂对合成气转化为低碳混合醇的催化性能,探讨不同助剂(包括K_2CO_3,KF,KCl等)对催化剂活性和选择性的影响,首次报道以K_2MoS_4作为前驱物料制得的MoS_x-K~+/SiO_2催化剂具有与(NH_4)_2MoS_4-K_2CO_3/SiO_2衍生得到的催化剂几乎一样高的催化活性和选择性,其结果为具有实用意义的催化剂的研制提供依据

Application of Accelerated Solvent Extraction Technique for Analysis of Active Components in Traditional Chinese Medicinal Herbs

以两种药材为研究实例,对加速溶剂萃取法(ASE)在中药材有效成分提取研究中的应用进行了简要介绍。采用正交试验法考察了提取丹参中丹酚酸B的提取条件(萃取温度、静态萃取时间、萃取溶剂以及料液比),确定了较好的实验条件。比较了ASE、水蒸气蒸馏法、超声波提取法及索氏提取法对木香挥发油的提取效果,结果表明ASE对木香挥发油的提取效果最好。The application of accelerated solvent extraction(ASE) technique for the Analysis of active components in traditional Chinese medicinal herbs was introduced by using two kinds of herbs as examples.The conditions including extraction temperature,static extraction time,the ratio of material to solvent and solvent of ASE for extraction of salvianolic acid B in Salvia miltiorrhiza were optimized by orthogonal experiments,and the optimal conditions were obtained.Different extraction methods(ASE,steam distillation,ultrasonic wave and Soxhlet extraction) were used to extract volatile oil in Aucklandia lappa Decne.Results of the comparative experiments indicated that ASE was the most effective method in this case.All the results from these studies demonstrate that ASE is indeed a powerful tool in the preparation of herbal extracts for downstream chromatographic analysis.青岛“2004将才计划”(04-3-JJ-11);; 共建生物医药研发测试中心(LS-05-KJZX-76)资

合成气制低碳混合醇耐硫钼基催化剂的制备研究

本文考察不同制备方法和制备程序制得之MoS_x-K~+/SiO_2催化剂对合成气转化为低碳混合醇的催化性能,探讨不同助剂(包括K_2CO_3,KF,KCl等)对催化剂活性和选择性的影响,首次报道以K_2MoS_4作为前驱物料制得的MoS_x-K~+/SiO_2催化剂具有与(NH_4)_2MoS_4-K_2CO_3/SiO_2衍生得到的催化剂几乎一样高的催化活性和选择性,其结果为具有实用意义的催化剂的研制提供依据

合成气制混合醇硫化钼基催化剂的谱学表征

利用XRD、XPS和TPD等谱学方法对合成气制混合醇硫化钼基催化剂活性相作了表征研究。实验结果表明,碱助的MoS_x-K~+/SiO_2催化剂工作表面生成了Mo-S-K新相,很可能是催化活性相;表面钼物种主要是Mo~(4+),硫除了以S~(2-)作为主要硫物种之外,还观测到低价硫阴离子物种S~-或[s—s]~(2-)。随着碱性钾盐助剂添加量的增加,[s—s]~(2-)物种的浓度下降,H_2吸附量相应减少,这同催化剂的选择性由烃向醇的转变有着密切的联系

Mimetic-Enzyme Fluoroimmunoassay for HBsAg Based on Thermal Phase Separating Technique

以聚n- 异丙基丙烯酰胺（PnIP） 作为免疫反应载体， 以铁酞菁作为HrP的新型模拟酶来标记抗人HbSAg 抗体， 建立了基于热相分离技术的夹心型荧光免疫分析HbSAg 的方法. HbSAg浓度在20 ~5000ng/Ml范围与体系的相对荧光强度呈良好线性关系. 检测限8ng/Ml.An anti-HBsAg antibody was conjugated to Poly-N-Isopropylacrylamide,and a novel polymer-mimetic enzyme fluoroimmunoassay method for the determination of HBsAg with sandwich format was proposed by using iron-tetrasulfonatophthalocyanine (FeTSPc) as a labelling reagent.The calibration graph for HBsAg was linear over the range of 20～5 000 ng/mL with a detection limit of 8 ng/mL.福建省自然科学基金!（9810004）;国家教育部博士点基

Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints

To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints.The samples were extracted using ASE for 10 min under 100 ℃ and 9.65 MPa,and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively.Based on this method,the HPLC fingerprints of Radix gentianae were established.Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value,three compounds in water phase were identified as gentiopicroside,asafetida acid and loganic acid.There is no report of the compounds in chloroform phase.The content of gentiopicroside and loganic acid in samples of different groups were determined,separately.The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint.The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90,however,the chloroform phase fingerprint congruence coefficients were within 0.62-0.99.This method can be used for determination of potent component in Radix gentianae and its quality control.Radix gentianae from different producing areas have the largest diversities,and the diversities embodied in the content of chloroform phase compounds.青岛“2004将才计划”(04-3-JJ-11);; 国家海洋局青年基金资助项目(2005602);; 崂山区政府区校共建生物医药研发测试中心资助(LS-05-KJZX-76)