Heterosis analysis on crossbreeding between different populations of small abalone Haliotis diversicolor

杂色鲍(HAlIOTIS dIVErSICOlOr)是中国南方主要的鲍养殖种类,近年来“东优1号“杂色鲍因较高的成活率优势已在产业上得到越来越广泛的推广养殖。该研究采用3x3完全双列杂交的方式,对杂色鲍“东优1号“群体、杂色鲍古雷群体和杂色鲍越南群体3个群体自繁与杂交后代的养殖性能进行跟踪,并对其杂种优势进行评估。结果表明,3个群体自繁后代的生长速度和成活率上存在显著差异(P<0.05),同时3个组合的生长速度与成活率的双亲杂种优势率值均不高,且2个单亲杂种优势率值之间有显著差异(P<0.05)。Small abalone Haliotis diversicolor is an important cultivable species in Southern China.A new variety,Dongyou No.1,is now widely cultured because of its high survival rate.We conducted a 3×3 complete diallel cross between three populations of small abalone H.diversicolor,which were Dongyou No.1,Gulei and Vietnam.During the crossbreeding,we compared the six reciprocal cross lines and three parental lines in terms of growth rate and survival rate at grow-out stage,and analyzed their heterosis.Significant difference in shell length and survival rate had been found,which indicated a potential genetic difference between the three populations(P<0.05).The magnitudes of heterosis was not high,and differed within the cross lines,for both positive and negative values could be observed.Moreover,the two single-parent heterosis were significantly different from each other(P<0.05).国家高技术研究发展计划(863计划)项目(2012AA10A412); 国家自然科学基金项目(31101896); 科技部星火计划项目(2011GA720001); 福建省科技计划项目(2011N0033;2011J05095

Effects of temperature on the growth rates and survival rates of Haliotis diversicolor among different populations

在16、20、24和28℃这4个设定的水温下,以杂色鲍(HAlIOTIS dIVErSICOlOr)日本群体、台湾群体、越南群体和“东优1号“为材料,进行为期40 d的养殖试验,对4个杂色鲍群体在不同温度下的壳长日增长率(lgr)和体重日增长率(Wgr)进行比较.结果表明,“东优1号“杂色鲍群体在28℃时的lgr和Wgr值分别达到162.23±15.79μM/d和46.52±6.89 Mg/d,在4个温度下的lgr和Wgr值都优于其他3个群体,台湾群体在较高温度(24和28℃)条件下的lgr和Wgr值要高于日本群体,而日本群体在较低温度(16和20℃)时的值则高于台湾群体.双因素方差分析的结果进一步表明,温度对杂色鲍不同群体的生长率指标具有显著影响.The small abalone,Haliotis diversicolor,is naturally distributed along the coastal waters of East Asia and is a commercially important species cultured along the coast of southern China.The current study genetically characterized four different small abalone populations(Japan,Taiwan,Vietnam,and "Dong You No.1") that were reared at 16,20,24,and 28℃ for 40 days,and the values of length growth rate(LGR) and weight growth rate(WGR) were investigated.The study revealed that the Dong You No.1 and the Vietnam populations had the highest and lowest values of LGR and WGR,respectively.Dong You No.1 reached 162.23±15.79 μm/d for LGR and 46.52±6.89 mg/d for WGR at 28℃.In terms of LGR and WGR,the Taiwan population performed better than the Japan population at higher temperatures(24 and 28℃),while the Japan population performed better than the Taiwan population at lower temperatures(16 and 20℃).The impact of population and temperature on LGR and WGR was investigated by two-way analysis of variance.The current study indicated that the impact of population and temperature on growth were both highly significant(p<0.01),and a significant association(p<0.05) between population and temperature was also found.The results provide useful information for optimal growth conditions for the small abalone industry.国家863计划资助项目(2006AA10A407;2010AA10A401);广东省教育部产学研结合资助项目(2009B090300159);科技部农业科技成果转化资金资助项目(2010GB23600665