Study on Purification of Chinese Narcissus Viruses

研究建立了一个差速离心和蔗糖阶梯式密度离心的分离法 ,对感染水仙叶片的有关病毒进行分离、纯化 .发现有 3种不同的病毒颗粒 ,大小分别为 4 80～ 5 5 0× 12～ 13nm ;70 0～ 96 0× 12～13nm ;180～ 2 5 0× 12～ 13nm .SDS电泳发现 3种与病毒相关的蛋白质 .它们可能属于水仙黄条病毒、水仙花叶病毒和烟草脆裂病毒The present study establishes purification method of related viruses in infected leaves of narcissus by using disparity gradient centrifuge. The results show that there are three kinds of different particles of the viruses which are 480～550×12～13nm,800～960×12～13nm;and 180～250×12～13nm respectively.SDS eletropho esis analysis indicates that there are three kinds of proteins related to Narcissus viruses, which probably belong to narcissus yellow stripe virus, narcissus mosaic virus and tobravirus.福建省自然科学基金资助课题 (C970 0 1号 );; 漳州市科委资助项