Study on the Polysaccharides from Phellinus Linteus on Sperm Abnormal- ity and Chromosome Aberration in Testicle Cell of Mice

目的通过小鼠精子畸变和睾丸细胞染色体畸变试验，探讨桑黄多糖对雄性小鼠的生殖毒性。方法试验设三个剂量组为2．5g．kg^-1、5．Og·kg^-1、10．Og·kg^-1Bw，经口灌胃进行试验，取小鼠睾丸制备标本，分别观察并计算各剂量组小鼠的精予畸变和睾丸细胞染色体畸变率。结果桑黄多糖各剂量组小鼠的精子和睾丸细胞染色体畸变率与空白对照均无显著性差异（P〉0．05），而环磷酰胺与空白对照组比较有显著性差异（P〈O．01）。结论桑黄多糖各剂量组对雄性小鼠无生殖毒性作用。OBJECTIVE To explore the reproductive toxic effect of the Polysaccharides from Phellinus Linteus on sperm abnormality and chromosome aberration in testicle cell test in male mice. METHODS The Polysaccha- rides from Phellinus Linteus were intragastrical administration at does of 2. 5g · kg^-1,5.0g · kg^-1 and 10. 0g · kg^-1 ,then the rates of sperm abnormality and chromosome aberration in testMe cell were calculated. RESULTS There was no significant difference between the solvent conrail group and the treated group of the rates of sperm abnormality and chromosome aberration in testicle cell （P 〉 0.05 ） , while significant difference was observed between the positive control group and the solvent control group（P 〈 0.01 ）. CONCLUSION The Polysaccharides from Phellinus Linte- us does not induce reproductive toxicity in male mice

Observation on the Clinical Effect of Shiduqing Combined with Ebastine in the Treatment of Atopic Dermatitis

目的观察湿毒清和依巴斯汀联合应用治疗特应性皮炎的临床疗效以及治疗前后外周血EOS、血清Ig E水平的变化情况。方法将我科94例特应性皮炎患者随机分为治疗组和对照组,每组47例,对照组患者使用依巴斯汀口服,治疗组患者加用湿毒清胶囊口服,观察治疗后对照组和治疗组的临床疗效及外周血EOS、血清Ig E的水平变化。结果对照组患者治疗总有效率为63.83%,治疗组总有效率为93.62%,具有显著性差异(P<0.05),治疗后治疗组血清Ig E水平与治疗前及对照组相比均呈明显下降趋势(P<0.05),具有统计学意义。结论湿毒清联合依巴斯汀口服治疗特应性皮炎比单纯口服依巴斯汀的治疗效果更加显著

探析他汀类联合心血管药物治疗冠心病的疗效

目的分析他汀类联合心血管药物治疗冠心病的临床疗效及对不良反应发生率的影响。方法将到我院诊治的166例冠心病患者选入本次研究,设立研究组、常规组,入院号为单数的患者归入研究组,双数的患者归入常规组,每组有83例,两组均应用常规心血管药物治疗,研究组在常规治疗的基础上结合瑞舒伐他汀治疗,观察两组患者的治疗效果、不良反应发生情况,对两组治疗前后的三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平进行统计。结果统计分析两组治疗有效率发现,研究组为98.80%,高于常规组的86.75%;对比不良反应发生情况,研究组不良反应率低于常规组(3.61%<12.05%);研究组治疗后的TG、TC、LDL-C、HDL-C各项水平改善更显著,P <0.05。结论他汀类联合心血管药物治疗冠心病的临床疗效优秀,不良反应发生情况较少

Effectivity and safety of three common antisyphilic drugs in treatment of early syphilis

目的观察3种驱梅药物治疗早期梅毒的临床治疗效果。方法选取2007年1月~2013年3月厦门大学附属第一医院门诊收治的早期梅毒患者共90例。随机将入选患者分为3组,分别是苄星青霉素组,克拉霉素组以及阿奇霉素组。观察比较3组患者的治疗效果,包括皮损的改善起效时间、皮损的痊愈时间及TruST转阴情况。结果 3组患者驱梅药物在一期梅毒治疗的平均皮损起效时间显示,苄星青霉素组与克拉霉素组、克拉霉素组与阿奇霉素组间比较差异均有统计学意义(T=14.603,P<0.05;T=12.682,P<0.05);而无论在一期梅毒治疗或二期梅毒治疗,3组患者的平均皮损痊愈的时间对比均没有显著性差异,二期梅毒患者平均皮损起效时间比较差异无统计学意义。苄星青霉素和克拉霉素的TruST转阴率为100%,阿奇霉素的转阴率为96.7%,但差异无统计学意义。结论苄星青霉素、克拉霉素和阿奇霉素的治疗效果相当,对于青霉素过敏的患者可以考虑采用克拉霉素和阿奇霉素进行早期梅毒治疗,能够取得满意的治疗效果。Objective To observe the clinical effect of three antisyphilic drugs in the treatment of early syphilis.Methods From January 2007 to March 2013,90 cases early syphilis in department of dermatology of our hospital were selected and randomly devided into three groups: benzathine penicillin group,clarithromycin group and azithromycin group.The efficacy including the onset time of improvement,recovery time of skin lesions and TRUST negative conversion rate were compared in three groups.Results The onset time of skin lesions improvement in patients with primary syphilis result showed that there was significant difference between benzathine penicillin and clarithromycin(t = 14.603,P < 0.05),clarithromycin and azithromycin(t = 12.682,P < 0.05).While in the treatment of primary syphilis and secondary syphilis,there were no significant differences among recovery time of skin lesions.And there were no significant differences the onset time of skin lesions improvement in treatment of secondary syphilis among three groups.The TRUST negative conversion rates of benzathine penicillin and clarithromycin both were 100%,and azithromycin was 96.7%,with no significant difference.Conclusion The antisyphilic efficacy of three drugs are similar.The patients allergic to penicillin may be treated with clarithromycin or azithromycin,who would aquaire a satisfied result.中华医学会2014年度中青年医师扶持计划(20140519

Study on dissolution method of Vitamin B4 tablets

目的建立维生素b4片溶出度检测方法。方法采用桨法,以900 M l P H6.8磷酸盐缓冲溶液为溶出介质,温度37.0℃,转速为50 r/MIn,选取时间为45 MIn,经0.45μM微孔滤膜过滤,取续滤液,采用紫外分光光度法测定维生素b4片的溶出量,检测波长为262 nM,溶出限度为标示量的80%。结果维生素b4浓度在4~16μg/M l范围内与吸光度呈良好的线性关系,r=0.9999(n=5),平均回收率为100.1%,rSd=1.0%(n=9)。结论该方法简便、灵敏、准确,对不同生产企业的处方、工艺有一定考察作用,可用于维生素b4片的溶出度测定。Objective To establish a test method for determining the vitamin B4 tablets dissolution.Methods Paddle method was used to 900 m L p H6.8 phosphate buffer solution as the dissolution medium,the temperature of 37 ℃,the speed of 50 r/min,take time for 45 min.Taking the filtrate through the 0.45 μm microporous membrane filtration,vitamin B4 tablets dissolution quantity determined by ultraviolet spectrophotometry,determined the wavelength of 262 nm,dissolution limit for 80% of the labeled amount.Results Vitamin B4 concentration had a good linear relationship with the absorbance in 4 ~ 16 μg/m L,r = 0.9999( n = 5),the average recovery was 100.1%,RSD = 1.0%( n = 9).Conclusion The method is simple,sensitive and accurate,has certain effect on prescription and study of the process of different production enterprises,can be used for the dissolution determination of vitamin B4 tablets

血液透析室护士身心健康状况与应对方式的关系研究

目的通过对血液透析室护士身心健康状况与应对方式的调查研究,促进护士心理行为良性发展,提高其身心健康水平。方法采用一般资料调查表、康奈尔健康问卷、简易应对方式量表对269名血液透析室护士进行问卷调查。结果血液透析室护士最常见的躯体健康问题是眼和耳(24.67%),其次是消化系统(18.26%),最常见的心理健康问题是不适应(19.42%),身心健康阳性率为15.61%,心理健康阳性率为7.43%。年龄越大、工作年限越长,护士的心理健康水平越高,消极应对得分明显高于常模,消极应对得分与身心健康状况得分呈正相关(P<0.01)。结论血液透析室护士身心健康状况不容乐观,既要提高工作能力也要注重心理调节能力的培养,促进护士形成积极的心理反应模式,尤其是年轻护士,应学会和运用积极的应对策略,保持良好的身心健康状况。厦门市科技计划项目(3502Z20144041

鳗鲡出血性开口病的病理学研究

本文报告了我国发生的一种鳗鲡病毒病──鳗鲡出血性开口病的组织病理变化：肝、肾、脾脏组织出血、细胞变性，骨质内有大县白细胞浸润，肝、肾、脾脏细胞超微结构病理变化明显，肾、脾脏造血组织和外周血细胞出现核染色质边集、奇异形核，大量髓鞘样结构、自噬体和自噬溶酶体等，并可见类似凋亡细胞及调亡小体结构和邻近细胞内吞噬体增多现象。根据骨组织中白细胞浸润及器官和血液中部分细胞结构已出现异型性特征，作者认为，鳗鲡出血性开口病可能有癌变的趋势

鳗鲡出血性开口病的病理学研究

本文报告了我国发生的一种鳗鲡病毒病－－鳗鲡出血性开口病的组织病理变化：肝、肾、脾脏组织出血、细胞变性、骨质内有大量白细胞浸润、肝、肾、脾脏细胞超微结构病理变化明显，肾、脾脏造血组织和外周血细胞出现核染色质边集、奇异形核、大量髓鞘样结构，自噬体和自噬溶酶体等，并可见类似凋亡细胞及凋亡小体结构和邻近细胞内吞噬体增多现象。根据骨组织中白细胞浸润及器官和血液中部分细胞结构已出现异型性特征，作者认为，鳗鲡

养殖乌鳢类立克次体感染的组织病理学研究

本文系统报道了养殖乌鳢类立克次体(Rickettsia-like organism,RLO)感染的各器官(脑、眼、鳃、心脏、头肾、肝、胰腺组织、脾、肾、肠和卵巢)的组织病理变化,探讨了炎症发展的基本规律。感染乌鳢病理解剖学特征和最具病理诊断意义的是体内各器官普遍出现的白色结节。这些结节的显微结构为肉芽肿炎症即一种慢性增生性炎症。在严重病变的肾脏,由于组织坏死区域较大和周围明显的细胞增生形成了境界较为清楚的巨大&ldquo;肉瘤&rdquo;状肿物。内脏器官的血管(特别是造血器官的血管)出现明显纤维素性血栓、混合血栓、弥散性血管

Variation in Biochemical Components of Ovary During the Ovarian Maturation of Marsupenaeus japonicus Under Different Conditions

在水温(23.5±1.5)℃,PH 7.0~8.0,盐度30~32,光强约为1 000lX的条件下,对来自台湾海峡的日本囊对虾(MArSuPEnAEuS JAPOnICuS)(体质量为(83.26±1.47)g/Ind)进行饥饿和切除眼柄的处理,研究亲虾性腺发育及卵巢发育过程中葡萄糖、胆固醇和甘油三酯的质量浓度变化情况.采用形态解剖称量法和酶学法测定亲虾卵巢发育过程葡萄糖、胆固醇和甘油三酯的质量浓度变化情况.结果表明:葡萄糖的质量浓度在对照组和切除眼柄组中呈现上升趋势,而在饥饿组中处于很低水平,交并组则是前2次取样有上升趋势,后2次取样时质量浓度下降;胆固醇的质量浓度在对照组和切除眼柄组积累呈显著递增趋势,饥饿组和交并组胆固醇质量浓度先上升后下降;甘油三酯的质量浓度将切除眼柄组与其余3组相比,整个实验期间都维持较高水平,但与对照组差异不太显著(P>0.05),饥饿组和交并组先上升后极显著降低(P0.05),and starvation group′s and starvation-eyestalk ablation group′s increased at first,then significantly decreased(p<0.01).The contents of glucose,cholesterol and triglyceride in eyestalk ablation group increased to some extent during the reproduction period.These results suggest that the variation in biochemical components and starvation and eyestalk ablation are deeply involved in ovarian maturation in Marsupenaeus japonicus broodstock.国家高技术研究发展计划(863)项目(2006AA10A406); 福建省科技计划重点项目(2012N0030