Intelligent Vibration Signal Diagnostic System Using Artificial Neural Network

[[abstract]]In this paper artificial neural network (ANN) technologies and analytical models have been investigated and incorporated to increase the effectiveness and efficiency of machinery self diagnostic system. Several advanced vibration trending methods have been studied and used to quantify machine operating conditions. An on-line, multi-channel condition monitoring procedure has been developed and coded. The major technique used for self diagnostic is a modified ARTMAP neural network. The objective is to provide a rigid solution for condition-based intelligent self diagnostic system.[[conferencetype]]國際[[conferencedate]]20091010~20091011[[iscallforpapers]]Y[[conferencelocation]]Changsha, Huna

Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode

Objectives: Non-viral mediated plasmid DNA transfection by electroporation (EP) is an established method for gene transfection. In this study, the usefulness of direct EP at an intradermal (i.d.) site (DEP) with implanted electrodes to achieve a high protein expression level was investigated. In addition, DEP application with various intervals with a low application voltage was also evaluated to confirm its effect on protein expression. Methods: Green fluorescent protein (GFP)- and luciferase-encoding DNA were administrated, and GFP and luciferase were evaluated. Results: A higher protein expression level was observed after green fluorescent protein (GFP)- and luciferase-encoding DNA were delivered by i.d. injection followed by DEP application. When luciferase expression was observed with an in vivo imaging system, continuous expression was confirmed over 21 days after i.d. injection followed by DEP at 100 V. This approach provided increased gene expression levels compared with conventional EP methods via the stratum corneum layer. In addition, the effect of application voltage on luciferase expression was investigated; two-time applications (repeated DEP) at 20 V with 5 min intervals showed similar luciferase expression level to single DEP application with 100 V. Histological observations showed the skin became thicker after a single DEP at 100 V, whereas no apparent thickness changes were confirmed after repeated DEP at 20 V with 5 min intervals. Conclusions: This study revealed that direct i.d. voltage application achieved high protein expression levels even at low voltages. Skin is a promising administration site for DNA vaccines, so this approach may be effective for DNA vaccine delivery into skin tissue.Article number: 1219, This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)

繰り返す嘔吐と体重増加不良を認めた先天性食道裂孔ヘルニアの2か月男児例

othe

1 超速効型インスリン治療への転換 : 第1報「当院におけるペン型インスリン治療の現状と問題点」(第31回新潟糖尿病談話会)

departmental bulletin pape

おやじの会という 連携のかたち －兵庫教育大学三校園『おやじの会』の事例をもとに－

departmental bulletin pape